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ALCAM/CD166 Antibody

 (4 Publications)
 (1 Review)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Biotin
BAF656
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Carrier Free
AF656-SP
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ALCAM/CD166 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # AAB59499
Specificity
Detects human ALCAM/CD166 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse ALCAM/CD166 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALCAM
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Adhesion Blockade
Reviewed Applications
Read 1 Review rated 5
using
AF656 in the following applications:
Publications
Read Publications using
AF656 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALCAM/CD166 Antibody

  • activated leucocyte cell adhesion molecule
  • activated leukocyte cell adhesion moleculeFLJ38514
  • ALCAM
  • CD166 antigen
  • CD166
  • MEMDMGC71733

Background

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALCAM/CD166 Antibody (AF656)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, Western Blot.


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ICC
(1)
IHC
(1)
IHC-Fr
(1)
Western Blot
(1)
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Human
(2)
Mouse
(1)
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Review for ALCAM/CD166 Antibody (AF656) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF656:
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IHC-Fr
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen ALCAM/CD166 AF656
Enlarge
reviewed by:
Anonymous
 IHC-Fr Mouse 05/08/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedEmbryonic spinal cord
SpeciesMouse
LotCZC0618111

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Immunohistochemistry-Frozen ALCAM/CD166 AF656
Enlarge
 Anonymous
05/08/2019
Application: IHC-Fr
Species: Mouse
read this review

Bioinformatics

Gene Symbol ALCAM
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