ALCAM/CD166 Antibody

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ALCAM/CD166 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
  Trp28-Ala526
  Accession # AAB59499
• Specificity: Detects human ALCAM/CD166 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse ALCAM/CD166 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALCAM
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Adhesion Blockade
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALCAM/CD166 Antibody

• activated leucocyte cell adhesion molecule
• activated leukocyte cell adhesion moleculeFLJ38514
• ALCAM
• CD166 antigen
• CD166
• MEMDMGC71733

Background

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALCAM/CD166 Antibody (AF656)(5)

Publications using AF656

• S Gc, K Tuy, L Rickenback, R Jones, A Chakrabort, CR Miller, EA Beierle, VS Hanumanthu, AN Tran, JA Mobley, SL Bellis, AB Hjelmeland alpha2,6 Sialylation mediated by ST6GAL1 promotes glioblastoma growth JCI Insight, 2022-11-08;7(21):. 2022-11-08 [PMID: 36345944] (Western Blot, Human)
• C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal Floor-plate-derived netrin-1 is dispensable for commissural axon guidance Nature, 2017-04-26;0(0):. 2017-04-26 [PMID: 28445456] (IHC, Mouse)
• Gu , Zirong, Imai , Fumiyasu, Kim , In Jung, Fujita , Hiroko, Katayama , Kei ichi, Mori , Kensaku, Yoshihara , Yoshihir, Yoshida , Yutaka Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion. PLoS ONE, 2015-03-31;10(3):e0121550. 2015-03-31 [PMID: 25826454] (ICC)
• Jannie KM, Stipp CS, Weiner JA ALCAM regulates motility, invasiveness, and adherens junction formation in uveal melanoma cells. PLoS ONE, 2012-06-26;7(6):e39330. 2012-06-26 [PMID: 22745734] (Western Blot, Human)
• van den Brand M, Takes RP, Blokpoel-deRuyter M, Slootweg PJ, van Kempen LC Activated leukocyte cell adhesion molecule expression predicts lymph node metastasis in oral squamous cell carcinoma. Oral Oncol., 2010-04-03;46(5):393-8. 2010-04-03 [PMID: 20371201] (IHC-Fr, Human)

Review for ALCAM/CD166 Antibody (AF656) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 05/08/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Embryonic spinal cord
• Species: Mouse
• Lot: CZC0618111

Bioinformatics

• Gene Symbol: ALCAM
• Uniprot:
  • AAB59499()