AgRP/ART Antibody [Unconjugated]

AgRP/ART was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Goat Anti-Mouse AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF634) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

AgRP/ART Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse AgRP/ART
  Val25-Thr131
  Accession # P56473
• Specificity: Detects mouse AgRP in direct ELISAs. In direct ELISAs, less than 25% cross-reactivity with recombinant human AgRP is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AGRP

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1.7-5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AgRP/ART Antibody [Unconjugated]

• agouti related protein homolog (mouse)
• agouti-related protein
• AgRP
• Agrt
• AGRTMGC118963
• ART
• ARTagouti (mouse) related protein
• ASIP2

Background

Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuroprotein that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) action on MC-3 and MC-4 receptors (1-4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature mouse AgRP is a 111 amino acid (aa) polypeptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Within the C-terminal region, mouse AgRP shares 80% and 90% aa sequence identity with human and rat AgRP, respectively. It also shares 44% aa sequence identity with Agouti. As with Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP, however, is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones via a mechanism that does not involve melanocortin receptors (14).

1. Martin, N.M. et al. (2006) Peptides 27:333.
2. Fan, W. et al. (1997) Nature 385:165.
3. Ollmann, M.M. et al. (1997) Science 278:135.
4. Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
5. Shutter, J.R. et al. (1997) Genes Dev. 11:593.
6. Kiefer, L.L. et al. (1998) Biochemistry 37:991.
7. Jackson, P.J. et al. (2002) Biochemistry 41:7565.
8. Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
9. Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
10. Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
11. Small, C.K. et al. (2001) Diabetes 50:248.
12. Vink, T. et al. (2001) Mol. Psychiatry 6:325.
13. Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
14. Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265-266:108.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AgRP/ART Antibody (AF634)(5)

Publications using AF634

• S Kim, N Kim, S Park, Y Jeon, J Lee, SJ Yoo, JW Lee, C Moon, SW Yu, EK Kim Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balance Autophagy, 2019;0(0):1-21. 2019 [PMID: 31469345] (IHC-Fr, Mouse)
• Z Ou, Y Ma, Y Sun, G Zheng, S Wang, R Xing, X Chen, Y Han, J Wang, QR Lu, TJ Zhao, Y Chen A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body Metabolism Cell Rep, 2019;26(11):2984-2997.e4. 2019 [PMID: 30865888] (IHC, Mouse)
• S Fukuhara, H Nakajima, S Sugimoto, K Kodo, K Shigehara, H Morimoto, Y Tsuma, M Moroto, J Mori, K Kosaka, M Morimoto, H Hosoi High-fat diet accelerates extreme obesity with hyperphagia in female heterozygous Mecp2-null mice PLoS ONE, 2019;14(1):e0210184. 2019 [PMID: 30608967] (Western Blot, Mouse)
• QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like Behavior PLoS ONE, 2016;11(11):e0164716. 2016 [PMID: 27835635] (IHC, Mouse)
• Cortes-Campos C, Elizondo R, Carril C, Martinez F, Boric K, Nualart F, Garcia-Robles M MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus. PLoS ONE, 2013;8(4):e62532. 2013 [PMID: 23638108] (ICC, Rat)

Bioinformatics

• Gene Symbol: AGRP
• Uniprot:
  • P56473()