AgRP/ART Antibody [Unconjugated]

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AgRP/ART was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Goat Anti-Mouse AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF634) at 1.7 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

AgRP/ART Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse AgRP/ART
Val25-Thr131
Accession # P56473
Specificity
Detects mouse AgRP in direct ELISAs. In direct ELISAs, less than 25% cross-reactivity with recombinant human AgRP is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AGRP
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1.7-5 ug/mL
Publications
Read Publications using
AF634 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AgRP/ART Antibody [Unconjugated]

  • agouti related protein homolog (mouse)
  • agouti-related protein
  • AgRP
  • Agrt
  • AGRTMGC118963
  • ART
  • ARTagouti (mouse) related protein
  • ASIP2

Background

Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuroprotein that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) action on MC-3 and MC-4 receptors (1-4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature mouse AgRP is a 111 amino acid (aa) polypeptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Within the C-terminal region, mouse AgRP shares 80% and 90% aa sequence identity with human and rat AgRP, respectively. It also shares 44% aa sequence identity with Agouti. As with Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP, however, is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones via a mechanism that does not involve melanocortin receptors (14).

  1. Martin, N.M. et al. (2006) Peptides 27:333.
  2. Fan, W. et al. (1997) Nature 385:165.
  3. Ollmann, M.M. et al. (1997) Science 278:135.
  4. Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
  5. Shutter, J.R. et al. (1997) Genes Dev. 11:593.
  6. Kiefer, L.L. et al. (1998) Biochemistry 37:991.
  7. Jackson, P.J. et al. (2002) Biochemistry 41:7565.
  8. Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
  9. Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
  10. Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
  11. Small, C.K. et al. (2001) Diabetes 50:248.
  12. Vink, T. et al. (2001) Mol. Psychiatry 6:325.
  13. Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
  14. Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265-266:108.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AgRP/ART Antibody (AF634)(5)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, Western Blot.


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(1)
IHC
(2)
IHC-Fr
(1)
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(1)
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(4)
Rat
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Bioinformatics

Gene Symbol AGRP
Uniprot