AG-2/AGR2 Antibody

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Simple Western: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Simple Western lane view shows a specific band for AGR2 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed ...read more
Genetic Strategies: Knockdown Validated: AG-2/AGR2 Antibody [NBP2-27393] - Alterations of tumor-associated phenotypes in AG-2-silenced SNU-478 cells. AG-2 protein expression in SNU-478:VEC and SNU-478:KDs was ...read more
Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Staining of human adenocarcinoma of the colon stained with AGR2 antibody (2-5 ug/ml), ...read more
Orthogonal Strategies: Western Blot: AG-2/AGR2 Antibody [NBP2-27393] - AGR2 expression in cancer cell lines of the biliary tract. AGR2 mRNA levels in six biliary tract cancer cell lines were measured by real-time ...read more
Western Blot: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Detection of AGR2 using AGR2 antibody. MCF7 (A), Caco-2 (B) and human stomach lysate (C) probed with AGR2 antibody at ...read more
Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Staining of human adenocarcinoma of the breast stained with AGR2 antibody (2 ug/ml), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Formulation Size Price

AG-2/AGR2 Antibody Summary

Immunogen
This antibody was developed against synthetic peptides corresponding to amino acids 55-72 of human AGR2.
Specificity
Researchers are encouraged to use the sequence and accession number (NP_006399.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity of the antibody to their species of interest.The AGR2 antibody is specific to AGR2 and does not recognize AGR3 (Verma et al, 2012)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AGR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:100~
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 22945649). Use in Immunohistochemistry reported in scientific literature (PMID: 22945649).
Publications
Read Publications using
NBP2-27393 in the following applications:

  • IF
    1 publication
  • IHC
    1 publication
  • 1 publication
  • 9 publications
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Rat,Chimpanzee,Dog,Bovine,Xenopus,Chicken,Orangutan

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 ml PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for AG-2/AGR2 Antibody

  • AG2
  • AG-2
  • AG2member 17
  • AGR2
  • anterior gradient 2 homolog (Xenopus laevis)
  • anterior gradient homolog 2 (Xenopus laevis)
  • GOB-4
  • hAG-2
  • HPC8
  • PDIA17
  • secreted cement gland homolog

Background

hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR1 and AGR2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development (1). Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for AG-2/AGR2 Antibody (NBP2-27393)(15)

We have publications tested in 3 confirmed species: Human, Mouse, M.

We have publications tested in 6 applications: IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
IF
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(9)
IP
(1)
WB
(5)
All Applications
Filter By Species
Human
(7)
Mouse
(5)
M
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP2-27393 Applications Species
Pajdzik K, Wilamowski M, Zurawek D et al. Anterior gradient 2 promotes tumorigenesis through upregulation of CCAAT-enhancer binding protein beta and hypoxia-inducible factor-2 alpha and subsequent secretion of interleukin-6, interleukin-8, and vascular endothelial growth factor in the Caki-1 clea IUBMB Life Aug 1 2020 [PMID: 32593213]
Kim N, Kim HK, Lee K et al. Single-cell RNA sequencing demonstrates the molecular and cellular reprogramming of metastatic lung adenocarcinoma Nat Commun May 8 2020 [PMID: 32385277] (IHC-P, Human) IHC-P Human
Kim Jihye, Chung Joon-Yong, Kim Tae-Joong et al. Genomic Network-Based Analysis Reveals Pancreatic Adenocarcinoma Up-Regulating Factor-Related Prognostic Markers in Cervical Carcinoma. Frontiers in Oncology 2018 Oct 23 [PMID: 30406031] (IHC-P, Human) IHC-P Human
Mito K, Saito M, Morita K et al. Clinicopathological and prognostic significance of MUC13 and AGR2 expression in intraductal papillary mucinous neoplasms of the pancreas. Pancreatology Apr 3 2018 [PMID: 29650332] (Human) Human
Dumartin L, Alrawashdeh W, Trabulo SM et al. ER stress protein AGR2 precedes and is involved in the regulation of pancreatic cancer initiation. Oncogene. Dec 12 2016 [PMID: 27941872] (WB, Human) WB Human
Mizuuchi Y, Aishima S, Ohuchida K et al. Anterior gradient 2 downregulation in a subset of pancreatic ductal adenocarcinoma is a prognostic factor indicative of epithelial-mesenchymal transition Lab. Invest. 2014 Nov 24 [PMID: 25418581] (WB, IHC-P, Human)

Details:
AG-2 / AGR2 antibody used for IHC at 1:100 dilution on formalin-fixed paraffin-embedded human pancreatic ductal adenocarcinoma (PDAC) tissues - endogenous peroxidase blocked with methanol and 0.3% H2O2 for 30 min, antigen retrieval by boiling slides in a microwave in 10 mM citrate buffer pH 6.0 for 20 min, primary incubated at 4C On followed by labelling with biotin-free horseradish peroxidase enzyme-labeled polymer - DAB. Antibody also used for WB on Aspc-1 and Hs766T cells treated with AGR2-specific siRnA (FIGURE 5b). AGR2 WB also performed on pancreatic ductal adenocarcinoma / PDAC cells with pancreatic stellate cell /PSCs and compared with monocultures (FIGURE 6D).
WB, IHC-P Human
Kim Sj, Jun S, Cho Hy et al. Knockdown of anterior gradient 2 expression extenuates tumor-associated phenotypes of SnU-478 ampulla of Vater cancer cells BMC Cancer et al. 2014 Nov 08 [PMID: 25367337] (WB, Human)

Details:
AG-2/alpha-AGR2 antibody used for WB on lysates of human biliary tract cancer cell lines - SnU-478:VEC and AGR2-silenced SnU-478 cells/ SnU-478:KDs (Figure 2B), and in SnU-869 stable transfectants/AGR2 overexpression SnU-869 cells (Figure 4B)
WB Human
Gupta A, Wodziak D, Tun M et al. Loss of anterior gradient 2 (Agr2) expression results in hyperplasia and defective lineage maturation in the murine stomach. J Biol Chem. 2013 Feb 8 [PMID: 23209296] (IHC-P, M)

Details:
Product Cited: AGR2 (IMG-5279A). IHC (paraffin) in stomach from WT mice and a mouse model consisting of both constitutive and inducible conditional AGR2 null mice: Figs 2C, 3, 4, 6C&D, 7D. Note: The specificity of the AGR2 was validated by IHC (paraffin)
IHC-P M
Norris AM, Gore A, Balboni A et al. AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia. Oncogene. 2013 Aug 15 [PMID: 22945649] (WB, IP, IF, IHC, Mouse)

Details:
The IMG-5279A AGR2 was used for multiple techniques in a mouse model of pancreatic ductal adenocarcinoma where AGR2 was evaluaed as a putative oncogene. Techniques in the publication include WB, IP, IF and IHC. See publication and supplimentary data for a
WB, IP, IF, IHC Mouse
Verma S, Salmans ML, Geyfman M et al. The estrogen-responsive Agr2 gene regulates mammary epithelial proliferation and facilitates lobuloalveolar development. Dev Biol. 2012 Sep 15 [PMID: 22819674] (WB, Mouse)

Details:
Product Cited: AGR2 (IMG-5279A). 1. WB: Recombinant AGR2 and AGR2, Fig S1. The AGR2 antibody recognized recombinant AGR2 but not recombinant AGR3. 2. IHC (paraffin): Wildtype (WT), transgenic AGR2 and knockout AGR2 Mouse mammary glands, Figs 1B, 2B, 4D. N
WB Mouse
Show All 15 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol AGR2