Reactivity | Hu, Mu, Rt, Po, Ch, Fe, Ma, Pm, Rb, Bv(-)Species Glossary |
Applications | WB, DB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P |
Clone | 45M1 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Immunogen | M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient |
Localization | Cytoplasmic |
Specificity | This monoclonal antibody recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | MUC5AC |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. Use in IHC-P reported in scientific literature (PMID:34260916). |
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Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein A or G purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Lindsey Johnson |
IHC-P | Rat | 10/20/2021 |
Summary
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reviewed by:
Lei Nie |
IHC-P | Human | 04/04/2020 |
Summary
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reviewed by:
Xianghou Xia |
WB | Mouse | 12/27/2019 |
Summary
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reviewed by:
Junwei HOU |
WB | Mouse | 02/18/2019 |
Summary
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reviewed by:
Junwei HOU |
WB | Human | 10/17/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
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Spheroids vs. Organoids: Which 3D Cell Culture Model is Best for You? By Jennifer Jones, M.S.Spheroids and organoids are two words that, like “butter” and “margarine”, are often referred to interchangeably but have distinct meanings. The progression and adopt... Read full blog post. |
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Lindsey Johnson 10/20/2021 |
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Application: | IHC-P | |
Species: | Rat |
Lei Nie 04/04/2020 |
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Application: | IHC-P | |
Species: | Human |
Xianghou Xia 12/27/2019 |
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Application: | WB | |
Species: | Mouse |