MUC5AC Antibody (45M1)

Immunohistochemistry: MUC5AC Antibody (45M1) [NBP2-15196] - Representative micrographs and high magnification insets illustrating the airway cell population of terminal bronchioles from conditional Nedd4-2-/- mice and littermate controls after 3 months of doxycycline induction with anti-Muc5ac and anti-CCSP antibodies (n = 4/group). Scale bars, 60 and 15 um (insets). Image collected and cropped by CiteAb from the following publication //www.nature.com/articles/s41467-020-15743-6) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Detection Human stomach.

Flow Cytometry: MUC5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196PE (blue) and a matched isotype control NBP2-27287PE (orange). To block the secretion of Mucin 5AC, cells were treated with 3uM Monensin for 3 hours. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both Antibodies were conjugated to Phycoerythrin.

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded Rat stomach stained with MUC5AC Monoclonal Antibody (45M1).

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).

Flow Cytometry: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Staining of fixed (70% ethanol or 80% methanol) and permeabilized A549 cells

Flow (Intracellular): MUC5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196 (blue) and a matched isotype control NBP2-27287 (orange). To block the secretion of Mucin 5AC, cells were treated with 3uM Monensin for 3 hours. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).

• Reactivity: Hu, Mu, Rt, Po, Ch, Fe, Ma, Pm, Rb, Bv(-)
• Applications: WB, DB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 45M1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MUC5AC Antibody (45M1) Summary

• Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
• Localization: Cytoplasmic
• Specificity: This MAb recognizes the peptide core of gastric mucin M1 (>1,000 kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC5AC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Flow (Intracellular)
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  Use in IHC-P reported in scientific literature (PMID:34260916).

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34260916)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-32732)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for MUC5AC Antibody (45M1)

• gastric mucin
• leB
• lewis B blood group antigen
• major airway glycoprotein
• MUC5
• mucin 5, subtypes A and C, tracheobronchial/gastric
• mucin 5AC, oligomeric mucus/gel-forming pseudogene
• mucin 5AC, oligomeric mucus/gel-forming
• mucin-5 subtype AC, tracheobronchial
• mucin-5AC
• TBM
• tracheobronchial mucin

Background

Mucin 5, subtype AC (MUC5AC) is a gel-forming secreted mucin that functions as a protective barrier on surface of epithelial tissues (1-2). MUC5AC is expressed primarily by goblet cells of the surface epithelium, including in the stomach, respiratory tract, gall bladder, endocervix, and endometrium (1-3). Human MUC5AC is located on chromosome 11p15.5, consisting of 5654 amino acids and has a N-terminus, tandem-repeat region (TRR), and a C-terminus, with a theoretical molecular weight of ~585 kDa (1-4). More specifically, the N-terminus contains four von Willebrand factor type D domains, where D1 also has a leucine zipper pattern (1). The TRR is further divided into nine CysD domains and four PTS-rich tandem repeats (1). Finally, the C-terminus has a D4-, B-, C-, and CK-domain (1). The TRR is a site of heavy O-glycosylation that functions in gel and polypeptide formation (1,3).

Given that MUC5AC is the primary mucin produced and secreted by cells lining airway, it is understandable that its expression is dysregulated in a number of respiratory diseases (1,3,5,6). For instance, MUC5AC is overexpressed in asthma and the protein is also more highly glycosylated, specifically fucosylated, in the disease state (1,3,7). Additionally, its overproduction is a key feature in chronic obstructive pulmonary disease (COPD) contributing to mucosal blockage (6). Multiple pathways are associated with overproduction of MUC5AC including NFkappaB, the primary pathway for production and secretion, and also the MAPK and STAT6 pathways (3). In addition to conventional, synthetic therapeutics agents, researchers are exploring natural MUC5AC inhibitors such as flavonoids, glycosides, and steroids to treat associated disorders (3).

References

1. Krishn, S. R., Ganguly, K., Kaur, S., & Batra, S. K. (2018). Ramifications of secreted mucin MUC5AC in malignant journey: a holistic view. Carcinogenesis. https://doi.org/10.1093/carcin/bgy019

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of clinical medicine. https://doi.org/10.3390/jcm8091447

3. Samsuzzaman, M., Uddin, M. S., Shah, M. A., & Mathew, B. (2019). Natural inhibitors on airway mucin: Molecular insight into the therapeutic potential targeting MUC5AC expression and production. Life sciences. https://doi.org/10.1016/j.lfs.2019.05.041

4. Uniprot (P98088)

5. Li, J., & Ye, Z. (2020). The Potential Role and Regulatory Mechanisms of MUC5AC in Chronic Obstructive Pulmonary Disease. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules25194437

6. Bonser, L. R., & Erle, D. J. (2017). Airway Mucus and Asthma: The Role of MUC5AC and MUC5B. Journal of clinical medicine. https://doi.org/10.3390/jcm6120112

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MUC5AC Antibody (NBP2-15196)(13)

Publications using NBP2-15196

• Wohnhaas CT, Gindele JA, Kiechle T et al. Cigarette Smoke Specifically Affects Small Airway Epithelial Cell Populations and Triggers the Expansion of Inflammatory and Squamous Differentiation Associated Basal Cells Int J Mol Sci Jul 24 2021 [PMID: 34299265] (Flow)
  
  Details:
  This citation used the Alexa Fluor 405 format of this antibody.
• Hicks-Berthet J, Ning B, Federico A Et Al. Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis Cell reports Jul 13 2021 [PMID: 34260916] (IHC-P)
• Ostedgaard LS, Price MP, Whitworth KM et al. Lack of airway submucosal glands impairs respiratory host defenses Elife Oct 7 2020 [PMID: 33026343] (ICC, Porcine)
• Ancel J, Belgacemi R, Perotin JM et al. Sonic hedgehog signalling as a potential endobronchial biomarker in COPD Respir. Res. Aug 7 2020 [PMID: 32767976] (ICC/IF, Human)
• Zarei K, Stroik MR, Gansemer ND et al. Early pathogenesis of cystic fibrosis gallbladder disease in a porcine model Lab. Invest. Jul 27 2020 [PMID: 32719544] (IHC-P, Porcine)
• Duerr J, Leitz DHW, Szczygiel M et al. Conditional deletion of Nedd4-2 in lung epithelial cells causes progressive pulmonary fibrosis in adult mice Nat Commun Apr 24 2020 [PMID: 32332792] (IHC, Mouse)
• Belgacemi R, Luczka E, Ancel J Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD EBioMedicine Dec 23 2019 [PMID: 31877414] (IHC-P, IHC, Human)
• Ladel S, Schlossbauer P et al. Improved In Vitro Model for Intranasal Mucosal Drug Delivery: Primary Olfactory and Respiratory Epithelial Cells Compared with the Permanent Nasal Cell Line RPMI 2650. Pharmaceutics Jan 8 2019 [PMID: 31374872] (Dot blot, Porcine)
• Rutledge EA, Parvez RK, Short KM et al. Morphogenesis of the kidney and lung requires branch-tip directed activity of the Adamts18 metalloprotease Dev. Biol. Jun 23 2019 [PMID: 31242448] (IHC-F, Mouse)
• Kobayashi H, Yoshida S, Shirasawa N et al. Expression and localization of aromatase in human gastric mucosa : Immunohistochemical study using biopsy materials. Histochem. Cell Biol. Aug 28 2018 [PMID: 30159783] (Human)
• Ostedgaard LS, Moninger TO, McMenimen JD et al. Gel-forming mucins form distinct morphologic structures in airways Proc. Natl. Acad. Sci. U.S.A. 2017 Jun 27 [PMID: 28607090] (ICC/IF, Porcine)
• Seidel V, Hoffmann R, Braun A et al. Distribution and morphology of Clara cells in common marmosets (Callithrix jacchus). J Med Primatol. 2013 Apr [PMID: 23473106]
• Meyerholz DK, Stoltz DA, Namati E et al. Loss of cystic fibrosis transmembrane conductance regulator function produces abnormalities in tracheal development in neonatal pigs and young children. Am J Respir Crit Care Med;182(10):1251-61. 2010 Nov 15. [PMID: 20622026]

Reviews for MUC5AC Antibody (NBP2-15196) (5)

Average Rating: 4.2

(Based on 5 reviews)

We have 5 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Verified Customer IHC-P Rat 10/20/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Stomach tissue
• Species: Rat
• Lot: 45M1

Comments

• Comments: 4% formaldehyde fixed paraffin sections

reviewed by: Verified Customer IHC-P Human 04/04/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: breast cancer tissue
• Species: Human
• Lot: 4586-1P1170303

reviewed by: Verified Customer WB Mouse 12/27/2019

Summary

• Application: Western Blot
• Sample Tested: EMT6 cells
• Species: Mouse
• Lot: 4586-1p1170303

reviewed by: Verified Customer WB Mouse 02/18/2019

Summary

• Application: Western Blot
• Sample Tested: CT26,EMT6 cells
• Species: Mouse
• Lot: 4586-1P1170303

reviewed by: Verified Customer WB Human 10/17/2018

Summary

• Application: Western Blot
• Sample Tested: AGS human gastric adenocarcinoma cell line,KATO-III human gastric carcinoma cell line
• Species: Human
• Lot: 4586-1P1170303

FAQs for MUC5AC Antibody (NBP2-15196). (Showing 1 - 1 of 1 FAQs).

1. I would be interested in buying the anti-Mucin 5AC (clone 45M1). However, the antibodies from Abcam (your competitor), also clone "45M1" does not work in the rat. On your datasheet, you announce your antibody "cross React" with different species, including rats. Can you please confirm me?
   • Our Mucin 5AC antibody NBP2-15196 has rat and other species listed as possible cross reactive species based on homology. The Abcam antibody of the same clone also lists rat as a potential species. Unlike Abcam, we will apply our guarantee to species that have 90% homology or higher.

Bioinformatics

• Gene Symbol: MUC5AC
• Uniprot:
  • P98088(Human)