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MUC5AC Antibody (45M1)

Images

 
Immunohistochemistry: MUC5AC Antibody (45M1) [NBP2-15196] - Representative micrographs and high magnification insets illustrating the airway cell population of terminal bronchioles from conditional Nedd4-2-/- mice and ...read more
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Detection Human stomach.
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded Rat stomach stained with MUC5AC Monoclonal Antibody (45M1).
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).
Conditional deletion of Nedd4-2 in lung epithelial cells causes peripheral airway remodeling with increased Muc5b production.a-d Representative micrographs and high magnification insets illustrating the airway cell ...read more
UroA/UAS03 enhance tight junction proteins in AhR-dependent manner. a HT29 cells were treated with vehicle (DMSO-0.01%)/UroA/UAS03 (50 μM) for 24 h. mRNA levels of Cytochrome P450 1A1 (Cyp1A1) was measured by RT ...read more
Ddx19 binds directly to MKL1 RPEL domain(a) Recruitment of Ipo beta and Ddx19 from Ipo beta -depleted NIH 3T3 cytoplasmic lysates by GST-RPEL. (−), NIH 3T3 cytoplasmic lysate transfected with control siRNAs; (+), Ipo ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Fe, Ma, Pm, Rb, Bv(-)Species Glossary
Applications WB, DB, Flow, ICC/IF, IHC
Clone
45M1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

MUC5AC Antibody (45M1) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-32732)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Localization
Cytoplasmic
Specificity
This monoclonal antibody recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC5AC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • Flow (Intracellular)
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in IHC-P reported in scientific literature (PMID:34260916).
Reviewed Applications
Read 5 Reviews rated 4.2
using
NBP2-15196 in the following applications:

Publications
Read Publications using
NBP2-15196 in the following applications:

Reactivity Notes

Hedgehog. Does not react with Bovine.

Use in Mouse reported in scientific literature (PMID:34260916)

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC5AC Antibody (45M1)

  • gastric mucin
  • leB
  • lewis B blood group antigen
  • major airway glycoprotein
  • MUC5
  • mucin 5, subtypes A and C, tracheobronchial/gastric
  • mucin 5AC, oligomeric mucus/gel-forming pseudogene
  • mucin 5AC, oligomeric mucus/gel-forming
  • mucin-5 subtype AC, tracheobronchial
  • mucin-5AC
  • TBM
  • tracheobronchial mucin

Background

Mucin 5, subtype AC (MUC5AC) is a gel-forming secreted mucin that functions as a protective barrier on surface of epithelial tissues (1-2). MUC5AC is expressed primarily by goblet cells of the surface epithelium, including in the stomach, respiratory tract, gall bladder, endocervix, and endometrium (1-3). Human MUC5AC is located on chromosome 11p15.5, consisting of 5654 amino acids and has a N-terminus, tandem-repeat region (TRR), and a C-terminus, with a theoretical molecular weight of ~585 kDa (1-4). More specifically, the N-terminus contains four von Willebrand factor type D domains, where D1 also has a leucine zipper pattern (1). The TRR is further divided into nine CysD domains and four PTS-rich tandem repeats (1). Finally, the C-terminus has a D4-, B-, C-, and CK-domain (1). The TRR is a site of heavy O-glycosylation that functions in gel and polypeptide formation (1,3).

Given that MUC5AC is the primary mucin produced and secreted by cells lining airway, it is understandable that its expression is dysregulated in a number of respiratory diseases (1,3,5,6). For instance, MUC5AC is overexpressed in asthma and the protein is also more highly glycosylated, specifically fucosylated, in the disease state (1,3,7). Additionally, its overproduction is a key feature in chronic obstructive pulmonary disease (COPD) contributing to mucosal blockage (6). Multiple pathways are associated with overproduction of MUC5AC including NFkappaB, the primary pathway for production and secretion, and also the MAPK and STAT6 pathways (3). In addition to conventional, synthetic therapeutics agents, researchers are exploring natural MUC5AC inhibitors such as flavonoids, glycosides, and steroids to treat associated disorders (3).

References

1. Krishn, S. R., Ganguly, K., Kaur, S., & Batra, S. K. (2018). Ramifications of secreted mucin MUC5AC in malignant journey: a holistic view. Carcinogenesis. https://doi.org/10.1093/carcin/bgy019

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of clinical medicine. https://doi.org/10.3390/jcm8091447

3. Samsuzzaman, M., Uddin, M. S., Shah, M. A., & Mathew, B. (2019). Natural inhibitors on airway mucin: Molecular insight into the therapeutic potential targeting MUC5AC expression and production. Life sciences. https://doi.org/10.1016/j.lfs.2019.05.041

4. Uniprot (P98088)

5. Li, J., & Ye, Z. (2020). The Potential Role and Regulatory Mechanisms of MUC5AC in Chronic Obstructive Pulmonary Disease. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules25194437

6. Bonser, L. R., & Erle, D. J. (2017). Airway Mucus and Asthma: The Role of MUC5AC and MUC5B. Journal of clinical medicine. https://doi.org/10.3390/jcm6120112

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, DB, Flow, ICC/IF, IHC

Publications for MUC5AC Antibody (NBP2-15196)(24)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 8 applications: Dot Blot, FLOW, ICC, ICC/IF, IF/IHC, IHC, IHC-F, IHC-P.


Filter By Application
Dot Blot
(1)
FLOW
(2)
ICC
(1)
ICC/IF
(2)
IF/IHC
(3)
IHC
(1)
IHC-F
(1)
IHC-P
(5)
All Applications
Filter By Species
Human
(4)
Mouse
(5)
Porcine
(4)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NBP2-15196 Applications Species
Tao X, Li J, He J et al. Pinellia ternata (Thunb.) Breit. Attenuates the allergic airway inflammation of cold asthma via inhibiting the activation of TLR4-medicated NF-kB and NLRP3 signaling pathway Journal of ethnopharmacology 2023-05-31 [PMID: 37268256]
Turner M, Cox K, Liu S et al. Specific Targeting and Labeling of Colonic Polyps in CPC-APC Mice with Mucin 5AC Fluorescent Antibodies: A Model for Detection of Early Colon Cancer Current Issues in Molecular Biology 2023-04-11 [PMID: 37185743] (IHC) IHC
Jung MA, Song HK, Jo K et al. Gleditsia sinensis Lam. aqueous extract attenuates nasal inflammation in allergic rhinitis by inhibiting MUC5AC production through suppression of the STAT3/STAT6 pathway Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-05-01 [PMID: 36921533] (IHC-P, Mouse)

Details:
Dilution used in IHC-P 1:1000
IHC-P Mouse
Bu ML, Li MH, Feng M et al. Poly(I:C) Exacerbates Airway Goblet Cell Hyperplasia and Lung Inflammation in HDM-Exposed Balb/C Mice by YAP/FOXM1 Pathway International archives of allergy and immunology 2023-02-23 [PMID: 36822170]
Koh K, Bonser L, Eckalbar W et al. Genomic characterization and therapeutic utilization of IL-13-responsive sequences in asthma Cell Genomics 2022-12-01 [PMID: 36777184] (FLOW, Human) FLOW Human
Pyun BJ, Jo K, Lee JY et al. Caesalpinia sappan Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells Antioxidants (Basel, Switzerland) 2022-11-15 [PMID: 36421442] (IHC-P, Mouse)

Details:
Female BALB/c mouse nasal tissue. Dilution of 1:1000 was used
IHC-P Mouse
Xu K, Liu XN, Zhang HB et al. Tear film instability is associated with weakened colocalization between occludin and MUC5AC in scopolamine-induced dry eye disease (DED) rats International ophthalmology 2023-02-01 [PMID: 35908134]
Tao X, Liu H, Xia J et al. Processed product (Pinelliae Rhizoma Praeparatum) of Pinellia ternata (Thunb.) Breit. Alleviates the allergic airway inflammation of cold phlegm via regulation of PKC/EGFR/MAPK/PI3K-AKT signaling pathway Journal of ethnopharmacology 2022-09-15 [PMID: 35688257] (IF/IHC, Mouse) IF/IHC Mouse
Wohnhaas CT, Gindele JA, Kiechle T et al. Cigarette Smoke Specifically Affects Small Airway Epithelial Cell Populations and Triggers the Expansion of Inflammatory and Squamous Differentiation Associated Basal Cells Int J Mol Sci 2021-07-24 [PMID: 34299265] (FLOW)

Details:
This citation used the Alexa Fluor 405 format of this antibody.
FLOW
Hicks-Berthet J, Ning B, Federico A Et Al. Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis Cell reports 2021-07-13 [PMID: 34260916] (IHC-P) IHC-P
Show All 24 Publications.

Reviews for MUC5AC Antibody (NBP2-15196) (5) 4.25

Average Rating: 4.2
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NBP2-15196:
Filter by Applications
IHC-P
(2)
WB
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MUC5AC NBP2-15196
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5
reviewed by:
Verified Customer
IHC-P Rat 10/20/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedStomach tissue
SpeciesRat
Lot45M1

Comments

Comments4% formaldehyde fixed paraffin sections
Immunohistochemistry-Paraffin MUC5AC NBP2-15196
Enlarge
4
reviewed by:
Verified Customer
IHC-P Human 04/04/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast cancer tissue
SpeciesHuman
Lot4586-1P1170303
Western Blot MUC5AC NBP2-15196
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 12/27/2019
View

Summary

ApplicationWestern Blot
Sample TestedEMT6 cells
SpeciesMouse
Lot4586-1p1170303
Western Blot MUC5AC NBP2-15196
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 02/18/2019
View

Summary

ApplicationWestern Blot
Sample TestedCT26,EMT6 cells
SpeciesMouse
Lot4586-1P1170303
Western Blot MUC5AC NBP2-15196
Enlarge
4
reviewed by:
Verified Customer
WB Human 10/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedAGS human gastric adenocarcinoma cell line,KATO-III human gastric carcinoma cell line
SpeciesHuman
Lot4586-1P1170303

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC5AC Antibody (NBP2-15196). (Showing 1 - 1 of 1 FAQs).

  1. I would be interested in buying the anti-Mucin 5AC (clone 45M1). However, the antibodies from Abcam (your competitor), also clone "45M1" does not work in the rat. On your datasheet, you announce your antibody "cross React" with different species, including rats. Can you please confirm me?
    • Our Mucin 5AC antibody NBP2-15196 has rat and other species listed as possible cross reactive species based on homology. The Abcam antibody of the same clone also lists rat as a potential species. Unlike Abcam, we will apply our guarantee to species that have 90% homology or higher.

Secondary Antibodies

 

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Recent Reviews

4.2
5
1
4
4
3
0
2
0
1
0

Verified Customer
10/20/2021
Application: IHC-P
Species: Rat

Verified Customer
04/04/2020
Application: IHC-P
Species: Human

Verified Customer
12/27/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MUC5AC
Uniprot