MUC5AC Antibody (45M1)

Images

 
Western Blot: MUC5AC Antibody (45M1) [NBP2-15196] - AGS human gastric adenocarcinoma and KATO-III human gastric carcinoma cell line lysates. Image from verified customer review.
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).
Flow Cytometry: MUC5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196PE (blue) and a matched isotype control NBP2-27287PE (orange). To block ...read more
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Detection Human stomach.
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded Rat stomach stained with MUC5AC Monoclonal Antibody (45M1).
Flow Cytometry: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Staining of fixed (70% ethanol or 80% methanol) and permeabilized A549 cells
Flow (Intracellular): MUC5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196 (blue) and a matched isotype control NBP2-27287 (orange). To block ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Fe, Pm, Rb, Bv(-)Species Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
45M1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

MUC5AC Antibody (45M1) Summary

Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Localization
Cytoplasmic
Specificity
This MAb recognizes the peptide core of gastric mucin M1 (>1,000 kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC5AC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5 ug/mL
  • Immunohistochemistry-Paraffin 0.5 - 1 ug/mL
  • Flow (Intracellular)
Application Notes
Human Chromosome Location: 11p15.5
Immunohistochemistry-Frozen and Western Blot applications cited in literature. This MUC5AC antibody (45M1) is validated for WB from a verified customer review. The 7-mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Reviewed Applications
Read 4 Reviews rated 4
using
NBP2-15196 in the following applications:

Publications
Read Publications using
NBP2-15196 in the following applications:

Reactivity Notes

Hedgehog

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS, 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for MUC5AC Antibody (45M1)

  • gastric mucin
  • leB
  • lewis B blood group antigen
  • major airway glycoprotein
  • MUC5
  • mucin 5, subtypes A and C, tracheobronchial/gastric
  • mucin 5AC, oligomeric mucus/gel-forming pseudogene
  • mucin 5AC, oligomeric mucus/gel-forming
  • mucin-5 subtype AC, tracheobronchial
  • mucin-5AC
  • TBM
  • tracheobronchial mucin

Background

MUC5AC (gastric mucin) belongs to the gel-forming mucin family of glycoproteins, the major components of the protective mucus layer on the mucosal surfaces of epithelial tissues. Mucus protects the tissue surface from mechanical damage, stabilizes the luminal microenvironment, and traps pathogens including bacteria and viruses for mucociliarly clearance. MUC5AC, like other family members, is differentially expressed. MUC5AC is expressed in airway and gastric epithelial cells and highly expressed in colorectal carcinomas. Although not normally detected by antibody in adult colon, MUC5AC is detectable in fetal colon. It is thought that positive MUC5AC antibody staining in colorectal cancer may be due to the resurgence of MUC5AC expression or the unmasking of an embryonic MUC5AC antibody reactive epitope during tumorigenesis. There are qualitative and quantitative alterations of MUC5AC expression in association with various pathological conditions. For example, S dysentariae infection induced MUC5AC expression in HT29 cells which was inhibited by polymyxin B pretreatment (Raja et al, 2011). Antibody studies identified elevated MUC5AC levels in benign and malignant gallbladder lesions (Xiong et al, 2011). On the other hand, MUC5AC expression is decreased or has abnormal glycosylation in the goblet cells of conjunctiva in a number of eye inflammatory conditions including Sjogren, Steven-Johnson and dry eye syndromes (Contreras-Ruiz, 2012). The MUC5AC clone 45M1 antibody is widely used. For example, it has been used as part of an antibody panel to help distinguish esophageal adenocarcinoma (MUC5AC/+) from squamous cell carcinoma (MUC5AC/-) (DiMaio et al, 2012)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Po, Ch, Fe, Pm, Rb, Bv(-)
Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for MUC5AC Antibody (NBP2-15196)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-F, IHC-P.


Filter By Application
ICC/IF
(1)
IHC
(2)
IHC-F
(1)
IHC-P
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Porcine
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP2-15196 Applications Species
Zarei K, Stroik MR, Gansemer ND et al. Early pathogenesis of cystic fibrosis gallbladder disease in a porcine model Lab. Invest. Jul 27 2020 [PMID: 32719544] (IHC-P, Porcine) IHC-P Porcine
Duerr J, Leitz DHW, Szczygiel M et al. Conditional deletion of Nedd4-2 in lung epithelial cells causes progressive pulmonary fibrosis in adult mice Nat Commun Apr 24 2020 [PMID: 32332792] (IHC, Mouse) IHC Mouse
Belgacemi R, Luczka E, Ancel J Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD EBioMedicine Dec 23 2019 [PMID: 31877414] (IHC-P, IHC, Human) IHC-P, IHC Human
Rutledge EA, Parvez RK, Short KM et al. Morphogenesis of the kidney and lung requires branch-tip directed activity of the Adamts18 metalloprotease Dev. Biol. Jun 23 2019 [PMID: 31242448] (IHC-F, Mouse) IHC-F Mouse
Kobayashi H, Yoshida S, Shirasawa N et al. Expression and localization of aromatase in human gastric mucosa : Immunohistochemical study using biopsy materials. Histochem. Cell Biol. Aug 28 2018 [PMID: 30159783] (Human) Human
Ostedgaard LS, Moninger TO, McMenimen JD et al. Gel-forming mucins form distinct morphologic structures in airways Proc. Natl. Acad. Sci. U.S.A. 2017 Jun 27 [PMID: 28607090] (ICC/IF, Porcine) ICC/IF Porcine
Seidel V, Hoffmann R, Braun A et al. Distribution and morphology of Clara cells in common marmosets (Callithrix jacchus). J Med Primatol. 2013 Apr [PMID: 23473106]
Meyerholz DK, Stoltz DA, Namati E et al. Loss of cystic fibrosis transmembrane conductance regulator function produces abnormalities in tracheal development in neonatal pigs and young children. Am J Respir Crit Care Med;182(10):1251-61. 2010 Nov 15. [PMID: 20622026]

Reviews for MUC5AC Antibody (NBP2-15196) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-15196:
Filter by Applications
IHC-P
(1)
WB
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MUC5AC NBP2-15196
Enlarge
reviewed by:
Anonymous
IHC-P Human 04/04/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested breast cancer tissue
SpeciesHuman
Lot4586-1P1170303
Western Blot MUC5AC NBP2-15196
Enlarge
reviewed by:
Anonymous
WB Mouse 12/27/2019
View

Summary

ApplicationWestern Blot
Sample TestedEMT6 cells
SpeciesMouse
Lot4586-1p1170303
Western Blot MUC5AC NBP2-15196
Enlarge
reviewed by:
Anonymous
WB Mouse 02/18/2019
View

Summary

ApplicationWestern Blot
Sample TestedEMT6 cells,CT26
SpeciesMouse
Lot4586-1P1170303
Western Blot MUC5AC NBP2-15196
Enlarge
reviewed by:
Anonymous
WB Human 10/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedAGS human gastric adenocarcinoma cell line,KATO-III human gastric carcinoma cell line
SpeciesHuman
Lot4586-1P1170303

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC5AC Antibody (NBP2-15196). (Showing 1 - 1 of 1 FAQs).

  1. I would be interested in buying the anti-Mucin 5AC (clone 45M1). However, the antibodies from Abcam (your competitor), also clone "45M1" does not work in the rat. On your datasheet, you announce your antibody "cross React" with different species, including rats. Can you please confirm me?
    • Our Mucin 5AC antibody NBP2-15196 has rat and other species listed as possible cross reactive species based on homology. The Abcam antibody of the same clone also lists rat as a potential species. Unlike Abcam, we will apply our guarantee to species that have 90% homology or higher.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-32732AF350
Alexa Fluor 405 NBP2-32732AF405
Alexa Fluor 488 NBP2-32732AF488
Alexa Fluor 532 NBP2-32732AF532
Alexa Fluor 594 NBP2-32732AF594
Alexa Fluor 647 NBP2-32732AF647
Alexa Fluor 700 NBP2-32732AF700
Alexa Fluor 750 NBP2-32732AF750
Allophycocyanin NBP2-32732APC
Allophycocyanin/Cy7 NBP2-32732APCCY7
Biotin NBP2-32732B
DyLight 350 NBP2-32732UV
DyLight 405 NBP2-32732V
DyLight 488 NBP2-32732G
DyLight 550 NBP2-32732R
DyLight 594 NBP2-32732DL594
DyLight 650 NBP2-32732C
DyLight 680 NBP2-32732FR
DyLight 755 NBP2-32732IR
FITC NBP2-32732F
HRP NBP2-32732H
Janelia Fluor 549 NBP2-32732JF549
Janelia Fluor 646 NBP2-32732JF646
PE NBP2-32732PE
PE/Atto594 NBP2-32732PEATT594
PE/Cy5.5 NBP2-32732PECY55
PE/Cy7 NBP2-32732PECY7
PerCP NBP2-32732PCP

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Recent Reviews

4
5
0
4
4
3
0
2
0
1
0

Anonymous
04/04/2020
Application: IHC-P
Species: Human

Anonymous
12/27/2019
Application: WB
Species: Mouse

Anonymous
02/18/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MUC5AC
Uniprot