MUC5AC Antibody (45M1)

Western Blot: MUC5AC Antibody (45M1) [NBP2-15196] - AGS human gastric adenocarcinoma and KATO-III human gastric carcinoma cell line lysates. Image from verified customer review.

Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).

Flow Cytometry: Mucin 5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196PE (blue) and a matched isotype control NBP2-27287PE (orange). To block the secretion of Mucin 5AC, cells were treated with 3uM Monensin for 3 hours. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both Antibodies were conjugated to Phycoerythrin.

Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Detection Human stomach.

Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded Rat stomach stained with MUC5AC Monoclonal Antibody (45M1).

Flow Cytometry: Mucin 5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Staining of fixed (70% ethanol or 80% methanol) and permeabilized A549 cells

Flow (Intracellular): Mucin 5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196 (blue) and a matched isotype control NBP2-27287 (orange). To block the secretion of Mucin 5AC, cells were treated with 3uM Monensin for 3 hours. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).

• Reactivity: Hu, Mu, Rt, Po, Ch, Fe, Pm, Rb, Bv(-)
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC-Fr, IHC-P
• Clone: 45M1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MUC5AC Antibody (45M1) Summary

• Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
• Localization: Cytoplasmic
• Specificity: This MAb recognizes the peptide core of gastric mucin M1 (>1,000 kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC5AC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/mL
  • Immunohistochemistry-Frozen 0.5 ug/mL
  • Immunohistochemistry-Paraffin 0.5 - 1 ug/mL
  • Flow (Intracellular)
• Application Notes: Human Chromosome Location: 11p15.5
  Immunohistochemistry-Frozen and Western Blot applications cited in literature.

Reactivity Notes

Hedgehog

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS with 0.05% BSA.
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for MUC5AC Antibody (45M1)

• gastric mucin
• leB
• lewis B blood group antigen
• major airway glycoprotein
• MUC5
• mucin 5, subtypes A and C, tracheobronchial/gastric
• mucin 5AC, oligomeric mucus/gel-forming pseudogene
• mucin 5AC, oligomeric mucus/gel-forming
• mucin-5 subtype AC, tracheobronchial
• mucin-5AC
• TBM
• tracheobronchial mucin

Background

MUC5AC (gastric mucin) belongs to the gel-forming mucin family of glycoproteins, the major components of the protective mucus layer on the mucosal surfaces of epithelial tissues. Mucus protects the tissue surface from mechanical damage, stabilizes the luminal microenvironment, and traps pathogens including bacteria and viruses for mucociliarly clearance. MUC5AC, like other family members, is differentially expressed. MUC5AC is expressed in airway and gastric epithelial cells and highly expressed in colorectal carcinomas. Although not normally detected by antibody in adult colon, MUC5AC is detectable in fetal colon. It is thought that positive MUC5AC antibody staining in colorectal cancer may be due to the resurgence of MUC5AC expression or the unmasking of an embryonic MUC5AC antibody reactive epitope during tumorigenesis. There are qualitative and quantitative alterations of MUC5AC expression in association with various pathological conditions. For example, S dysentariae infection induced MUC5AC expression in HT29 cells which was inhibited by polymyxin B pretreatment (Raja et al, 2011). Antibody studies identified elevated MUC5AC levels in benign and malignant gallbladder lesions (Xiong et al, 2011). On the other hand, MUC5AC expression is decreased or has abnormal glycosylation in the goblet cells of conjunctiva in a number of eye inflammatory conditions including Sjogren, Steven-Johnson and dry eye syndromes (Contreras-Ruiz, 2012). The MUC5AC clone 45M1 antibody is widely used. For example, it has been used as part of an antibody panel to help distinguish esophageal adenocarcinoma (MUC5AC/+) from squamous cell carcinoma (MUC5AC/-) (DiMaio et al, 2012)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MUC5AC Antibody (NBP2-15196)(4)

Publications using NBP2-15196

• Kobayashi H, Yoshida S, Shirasawa N et al. Expression and localization of aromatase in human gastric mucosa : Immunohistochemical study using biopsy materials. Histochem. Cell Biol. Aug 28 2018 [PMID: 30159783] (Human)
• Ostedgaard LS, Moninger TO, McMenimen JD et al. Gel-forming mucins form distinct morphologic structures in airways Proc. Natl. Acad. Sci. U.S.A. 2017 Jun 27 [PMID: 28607090] (ICC/IF, Porcine)
• Seidel V, Hoffmann R, Braun A et al. Distribution and morphology of Clara cells in common marmosets (Callithrix jacchus). J Med Primatol. 2013 Apr [PMID: 23473106]
• Meyerholz DK, Stoltz DA, Namati E et al. Loss of cystic fibrosis transmembrane conductance regulator function produces abnormalities in tracheal development in neonatal pigs and young children. Am J Respir Crit Care Med;182(10):1251-61. 2010 Nov 15. [PMID: 20622026]

Review for MUC5AC Antibody (NBP2-15196) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 10/17/2018

Summary

• Application: Western Blot
• Sample Tested: AGS human gastric adenocarcinoma cell line,KATO-III human gastric carcinoma cell line
• Species: Human
• Lot: 4586-1P1170303

FAQs for MUC5AC Antibody (NBP2-15196). (Showing 1 - 1 of 1 FAQs).

1. I would be interested in buying the anti-Mucin 5AC (clone 45M1). However, the antibodies from Abcam (your competitor), also clone "45M1" does not work in the rat. On your datasheet, you announce your antibody "cross React" with different species, including rats. Can you please confirm me?
   • Our Mucin 5AC antibody NBP2-15196 has rat and other species listed as possible cross reactive species based on homology. The Abcam antibody of the same clone also lists rat as a potential species. Unlike Abcam, we will apply our guarantee to species that have 90% homology or higher.

Bioinformatics

• Gene Symbol: MUC5AC
• Uniprot:
  • P98088(Human)