MUC5AC Antibody (45M1)

Images

 
Immunohistochemistry: MUC5AC Antibody (45M1) [NBP2-15196] - Representative micrographs and high magnification insets illustrating the airway cell population of terminal bronchioles from conditional Nedd4-2-/- mice and ...read more
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Detection Human stomach.
Flow Cytometry: MUC5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196PE (blue) and a matched isotype control NBP2-27287PE (orange). To block ...read more
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded Rat stomach stained with MUC5AC Monoclonal Antibody (45M1).
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).
Flow Cytometry: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Staining of fixed (70% ethanol or 80% methanol) and permeabilized A549 cells
Flow (Intracellular): MUC5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196 (blue) and a matched isotype control NBP2-27287 (orange). To block ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Fe, Ma, Pm, Rb, Bv(-)Species Glossary
Applications WB, DB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
45M1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

MUC5AC Antibody (45M1) Summary

Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Localization
Cytoplasmic
Specificity
This MAb recognizes the peptide core of gastric mucin M1 (>1,000 kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC5AC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • Flow (Intracellular)
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Western Blot
Application Notes
Use in IHC-P reported in scientific literature (PMID:34260916).
Reviewed Applications
Read 5 Reviews rated 4.2
using
NBP2-15196 in the following applications:

Publications
Read Publications using
NBP2-15196 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34260916)

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml (NBP2-32732).

Alternate Names for MUC5AC Antibody (45M1)

  • gastric mucin
  • leB
  • lewis B blood group antigen
  • major airway glycoprotein
  • MUC5
  • mucin 5, subtypes A and C, tracheobronchial/gastric
  • mucin 5AC, oligomeric mucus/gel-forming pseudogene
  • mucin 5AC, oligomeric mucus/gel-forming
  • mucin-5 subtype AC, tracheobronchial
  • mucin-5AC
  • TBM
  • tracheobronchial mucin

Background

Mucin 5, subtype AC (MUC5AC) is a gel-forming secreted mucin that functions as a protective barrier on surface of epithelial tissues (1-2). MUC5AC is expressed primarily by goblet cells of the surface epithelium, including in the stomach, respiratory tract, gall bladder, endocervix, and endometrium (1-3). Human MUC5AC is located on chromosome 11p15.5, consisting of 5654 amino acids and has a N-terminus, tandem-repeat region (TRR), and a C-terminus, with a theoretical molecular weight of ~585 kDa (1-4). More specifically, the N-terminus contains four von Willebrand factor type D domains, where D1 also has a leucine zipper pattern (1). The TRR is further divided into nine CysD domains and four PTS-rich tandem repeats (1). Finally, the C-terminus has a D4-, B-, C-, and CK-domain (1). The TRR is a site of heavy O-glycosylation that functions in gel and polypeptide formation (1,3).

Given that MUC5AC is the primary mucin produced and secreted by cells lining airway, it is understandable that its expression is dysregulated in a number of respiratory diseases (1,3,5,6). For instance, MUC5AC is overexpressed in asthma and the protein is also more highly glycosylated, specifically fucosylated, in the disease state (1,3,7). Additionally, its overproduction is a key feature in chronic obstructive pulmonary disease (COPD) contributing to mucosal blockage (6). Multiple pathways are associated with overproduction of MUC5AC including NFkappaB, the primary pathway for production and secretion, and also the MAPK and STAT6 pathways (3). In addition to conventional, synthetic therapeutics agents, researchers are exploring natural MUC5AC inhibitors such as flavonoids, glycosides, and steroids to treat associated disorders (3).

References

1. Krishn, S. R., Ganguly, K., Kaur, S., & Batra, S. K. (2018). Ramifications of secreted mucin MUC5AC in malignant journey: a holistic view. Carcinogenesis. https://doi.org/10.1093/carcin/bgy019

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of clinical medicine. https://doi.org/10.3390/jcm8091447

3. Samsuzzaman, M., Uddin, M. S., Shah, M. A., & Mathew, B. (2019). Natural inhibitors on airway mucin: Molecular insight into the therapeutic potential targeting MUC5AC expression and production. Life sciences. https://doi.org/10.1016/j.lfs.2019.05.041

4. Uniprot (P98088)

5. Li, J., & Ye, Z. (2020). The Potential Role and Regulatory Mechanisms of MUC5AC in Chronic Obstructive Pulmonary Disease. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules25194437

6. Bonser, L. R., & Erle, D. J. (2017). Airway Mucus and Asthma: The Role of MUC5AC and MUC5B. Journal of clinical medicine. https://doi.org/10.3390/jcm6120112

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Po, Ch, Fe, Ma, Pm, Rb, Bv(-)
Applications: WB, DB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for MUC5AC Antibody (NBP2-15196)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 6 applications: Dot blot, ICC, ICC/IF, IHC, IHC-F, IHC-P.


Filter By Application
Dot blot
(1)
ICC
(1)
ICC/IF
(2)
IHC
(2)
IHC-F
(1)
IHC-P
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Porcine
(4)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP2-15196 Applications Species
Hicks-Berthet J, Ning B, Federico A Et Al. Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis Cell reports Jul 13 2021 [PMID: 34260916] (IHC-P) IHC-P
Ostedgaard LS, Price MP, Whitworth KM et al. Lack of airway submucosal glands impairs respiratory host defenses Elife Oct 7 2020 [PMID: 33026343] (ICC, Porcine) ICC Porcine
Ancel J, Belgacemi R, Perotin JM et al. Sonic hedgehog signalling as a potential endobronchial biomarker in COPD Respir. Res. Aug 7 2020 [PMID: 32767976] (ICC/IF, Human) ICC/IF Human
Zarei K, Stroik MR, Gansemer ND et al. Early pathogenesis of cystic fibrosis gallbladder disease in a porcine model Lab. Invest. Jul 27 2020 [PMID: 32719544] (IHC-P, Porcine) IHC-P Porcine
Duerr J, Leitz DHW, Szczygiel M et al. Conditional deletion of Nedd4-2 in lung epithelial cells causes progressive pulmonary fibrosis in adult mice Nat Commun Apr 24 2020 [PMID: 32332792] (IHC, Mouse) IHC Mouse
Belgacemi R, Luczka E, Ancel J Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD EBioMedicine Dec 23 2019 [PMID: 31877414] (IHC-P, IHC, Human) IHC-P, IHC Human
Ladel S, Schlossbauer P et al. Improved In Vitro Model for Intranasal Mucosal Drug Delivery: Primary Olfactory and Respiratory Epithelial Cells Compared with the Permanent Nasal Cell Line RPMI 2650. Pharmaceutics Jan 8 2019 [PMID: 31374872] (Dot blot, Porcine) Dot blot Porcine
Rutledge EA, Parvez RK, Short KM et al. Morphogenesis of the kidney and lung requires branch-tip directed activity of the Adamts18 metalloprotease Dev. Biol. Jun 23 2019 [PMID: 31242448] (IHC-F, Mouse) IHC-F Mouse
Kobayashi H, Yoshida S, Shirasawa N et al. Expression and localization of aromatase in human gastric mucosa : Immunohistochemical study using biopsy materials. Histochem. Cell Biol. Aug 28 2018 [PMID: 30159783] (Human) Human
Ostedgaard LS, Moninger TO, McMenimen JD et al. Gel-forming mucins form distinct morphologic structures in airways Proc. Natl. Acad. Sci. U.S.A. 2017 Jun 27 [PMID: 28607090] (ICC/IF, Porcine) ICC/IF Porcine
Show All 12 Publications.

Reviews for MUC5AC Antibody (NBP2-15196) (5) 4.25

Average Rating: 4.2
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NBP2-15196:
Filter by Applications
IHC-P
(2)
WB
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MUC5AC NBP2-15196
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5
reviewed by:
Verified Customer
IHC-P Rat 10/20/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedStomach tissue
SpeciesRat
Lot45M1

Comments

Comments4% formaldehyde fixed paraffin sections
Immunohistochemistry-Paraffin MUC5AC NBP2-15196
Enlarge
4
reviewed by:
Verified Customer
IHC-P Human 04/04/2020
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested breast cancer tissue
SpeciesHuman
Lot4586-1P1170303
Western Blot MUC5AC NBP2-15196
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4
reviewed by:
Verified Customer
WB Mouse 12/27/2019
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Summary

ApplicationWestern Blot
Sample TestedEMT6 cells
SpeciesMouse
Lot4586-1p1170303
Western Blot MUC5AC NBP2-15196
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 02/18/2019
View

Summary

ApplicationWestern Blot
Sample TestedCT26,EMT6 cells
SpeciesMouse
Lot4586-1P1170303
Western Blot MUC5AC NBP2-15196
Enlarge
4
reviewed by:
Verified Customer
WB Human 10/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedAGS human gastric adenocarcinoma cell line,KATO-III human gastric carcinoma cell line
SpeciesHuman
Lot4586-1P1170303

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC5AC Antibody (NBP2-15196). (Showing 1 - 1 of 1 FAQs).

  1. I would be interested in buying the anti-Mucin 5AC (clone 45M1). However, the antibodies from Abcam (your competitor), also clone "45M1" does not work in the rat. On your datasheet, you announce your antibody "cross React" with different species, including rats. Can you please confirm me?
    • Our Mucin 5AC antibody NBP2-15196 has rat and other species listed as possible cross reactive species based on homology. The Abcam antibody of the same clone also lists rat as a potential species. Unlike Abcam, we will apply our guarantee to species that have 90% homology or higher.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-32732AF350
Alexa Fluor 405 NBP2-32732AF405
Alexa Fluor 488 NBP2-32732AF488
Alexa Fluor 532 NBP2-32732AF532
Alexa Fluor 594 NBP2-32732AF594
Alexa Fluor 647 NBP2-32732AF647
Alexa Fluor 700 NBP2-32732AF700
Alexa Fluor 750 NBP2-32732AF750
Allophycocyanin NBP2-32732APC
Allophycocyanin/Cy7 NBP2-32732APCCY7
Biotin NBP2-32732B
DyLight 350 NBP2-32732UV
DyLight 405 NBP2-32732V
DyLight 488 NBP2-32732G
DyLight 550 NBP2-32732R
DyLight 594 NBP2-32732DL594
DyLight 650 NBP2-32732C
DyLight 680 NBP2-32732FR
DyLight 755 NBP2-32732IR
FITC NBP2-32732F
HRP NBP2-32732H
Janelia Fluor 549 NBP2-32732JF549
Janelia Fluor 646 NBP2-32732JF646
PE NBP2-32732PE
PE/Atto594 NBP2-32732PEATT594
PE/Cy5.5 NBP2-32732PECY55
PE/Cy7 NBP2-32732PECY7
PerCP NBP2-32732PCP

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Recent Reviews

4.2
5
1
4
4
3
0
2
0
1
0

Verified Customer
10/20/2021
Application: IHC-P
Species: Rat

Verified Customer
04/04/2020
Application: IHC-P
Species: Human

Verified Customer
12/27/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MUC5AC
Uniprot