AG-2/AGR2 Antibody - Azide Free

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Western Blot: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - AGR2 expression in cancer cell lines of the biliary tract. AGR2 mRNA levels in six biliary tract cancer cell lines were measured by real-time RT-PCR. The AGR2 ...read more
Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Formalin-fixed, paraffin-embedded human adenocarcinoma of the breast stained with AGR2 antibody (2 ug/ml), peroxidase-conjugate and DAB ...read more
Western Blot: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Analysis of AGR2 using AGR2 antibody. MCF7 (A), Caco-2 (B) and human stomach lysate (C) probed with AGR2 antibody at 1:100. were used for this test. The ...read more
Western Blot: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Alterations of tumor-associated phenotypes in AG-2-silenced SNU-478 cells. AG-2 protein expression in SNU-478:VEC and SNU-478:KDs was detected by western blot ...read more
Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Staining of AGR2 using AGR2 antibody. Formalin-fixed, paraffin-embedded normal colon tissue probed with AGR2 antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Formalin-fixed, paraffin-embedded human adenocarcinoma of the colon stained with AGR2 antibody (2-5 ug/ml), peroxidase-conjugate and DAB ...read more
Simple Western: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Simple Western lane view shows a specific band for AGR2 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, IHC-Fr, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide Free

Order Details

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Catalog# & Formulation Size Price

AG-2/AGR2 Antibody - Azide Free Summary

Immunogen
This antibody was developed against synthetic peptides corresponding to amino acids 55-72 of human AGR2.
Specificity
Researchers are encouraged to use the sequence and accession number (NP_006399.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity of the antibody to their species of interest.The AGR2 antibody is specific to AGR2 and does not recognize AGR3 (Verma et al, 2012)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AGR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500~
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Simple Western 1:100
  • Western Blot 1:100
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Publications
Read Publications using NBP2-27392.

Reactivity Notes

Rat,Chimpanzee,Dog,Bovine,Xenopus,Chicken,Orangutan

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for AG-2/AGR2 Antibody - Azide Free

  • AG2
  • AG-2
  • AG2member 17
  • AGR2
  • anterior gradient 2 homolog (Xenopus laevis)
  • anterior gradient homolog 2 (Xenopus laevis)
  • GOB-4
  • hAG-2
  • HPC8
  • PDIA17
  • secreted cement gland homolog

Background

hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR1 and AGR2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development(1). Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AG-2/AGR2 Antibody (NBP2-27392)(8)


Showing Publications 1 - 8 of 8.
Publications using NBP2-27392 Applications Species
Gupta A, Wodziak D, Tun M et al. Loss of anterior gradient 2 (Agr2) expression results in hyperplasia and defective lineage maturation in the murine stomach. J Biol Chem. 2013 Feb 8 [PMID: 23209296]
Norris AM, Gore A, Balboni A et al. AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia. Oncogene. 2013 Aug 15 [PMID: 22945649]
Verma S, Salmans ML, Geyfman M et al. The estrogen-responsive Agr2 gene regulates mammary epithelial proliferation and facilitates lobuloalveolar development. Dev Biol. 2012 Sep 15 [PMID: 22819674]
Chen G, Korfhagen TR, Xu Y et al. SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production. J Clin Invest. 2009 Oct [PMID: 19759516]
Sitek B, Sipos B, Alkatout I et al. Analysis of the pancreatic tumor progression by a quantitative proteomic approach and immunhistochemical validation. J Proteome Res. 2009 Apr [PMID: 19714807]
Park SW, Zhen G, Verhaeghe C et al. The protein disulfide isomerase AGR2 is essential for production of intestinal mucus. Proc Natl Acad Sci U S A. 2009 Apr 28 [PMID: 19359471]
Chang J, Chance MR, Nicholas C et al. Proteomic changes during intestinal cell maturation in vivo. J Proteomics. 2008 Dec 2 [PMID: 18824147]
Wang Z, Hao Y, Lowe AW. The adenocarcinoma-associated antigen, AGR2, promotes tumor growth, cell migration, and cellular transformation. Cancer Res. 2008 Jan 15 [PMID: 18199544]

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Bioinformatics

Gene Symbol AGR2