| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27392 | |||
| BSA Free | NBP2-27393SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-17780SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05936SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27175SS | |||
| Azide and BSA Free | NBP2-80564 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6068-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05933SS | |||
![Simple Western: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Simple Western lane view shows a specific band for AGR2 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-Simple-Western-NBP2-27393-img0008.jpg "Simple Western: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Simple Western lane view shows a specific band for AGR2 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Genetic Strategies Validation. </b>Western Blot: AG-2/AGR2 Antibody [NBP2-27393] - Alterations of tumor-associated phenotypes in AG-2-silenced SNU-478 cells. AG-2 protein expression in SNU-478:VEC and SNU-478:KDs was detected by western blot analysis with beta-actin as a loading control. The result shown is a representative of three experiments. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-804), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-Knockdown-Validated-NBP2-27393-img0013.jpg)
![Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Staining of human adenocarcinoma of the colon stained with AGR2 antibody (2-5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-Immunohistochemistry-Paraffin-NBP2-27393-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: AG-2/AGR2 Antibody [NBP2-27393] - AGR2 expression in cancer cell lines of the biliary tract. AGR2 mRNA levels in six biliary tract cancer cell lines were measured by real-time RT-PCR. The AGR2 transcript levels were normalized against those of the GAPDH. Results shown are means +/- standard deviations of (1/2)CT of target - CT of GAPDH of three experiments. B. AGR2 protein expression was detected by western blot analysis with beta-actin as a loading control. The result shown is a representative of three experiments. Abbreviations: CBD, common bile duct; GB, gall bladder; AoV, ampulla of Vater; IHD, intrahepatic duct; HDB, hepatic duct bifurcation; WD, well differentiated; MD, moderately differentiated; PD, poorly differentiated [24]. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-804), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-Western-Blot-NBP2-27393-img0014.jpg)
![Western Blot: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Detection of AGR2 using AGR2 antibody. MCF7 (A), Caco-2 (B) and human stomach lysate (C) probed with AGR2 antibody at 1:100. were used for this test. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-Western-Blot-NBP2-27393-img0009.jpg)
![Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Staining of human adenocarcinoma of the breast stained with AGR2 antibody (2 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-Immunohistochemistry-Paraffin-NBP2-27393-img0003.jpg)
![Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody [NBP2-27393] - Staining of AGR2 using AGR2 antibody. Formalin-fixed, paraffin-embedded normal colon tissue probed with AGR2 antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/nbp2-27393_rabbit-polyclonal-ag-2-agr2-antibody-17720241044636.jpg)
![Western Blot: AG-2/AGR2 Antibody [NBP2-27393] - AGR2 expression in cancer cell lines of the biliary tract. A. AGR2 mRNA levels in six biliary tract cancer cell lines were measured by real-time RT-PCR. The AGR2 transcript levels were normalized against those of the GAPDH. Results shown are means ± standard deviations of (1/2)CT of target - CT of GAPDH of three experiments. B. AGR2 protein expression was detected by western blot analysis with beta -actin as a loading control. The result shown is a representative of three experiments. Abbreviations: CBD, common bile duct; GB, gall bladder; AoV, ampulla of Vater; IHD, intrahepatic duct; HDB, hepatic duct bifurcation; WD, well differentiated; MD, moderately differentiated; PD, poorly differentiated [24]. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27393_rabbit-polyclonal-ag-2-agr2-antibody-310202415384191.jpg)
![Western Blot: AG-2/AGR2 Antibody [NBP2-27393] - Alterations of tumor-associated phenotypes by overexpression of AGR2 in SNU-869 cells. A. AGR2 expression in the SNU-869 stable transfectants was verified by real-time RT-PCR. The AGR2 transcript level was normalized against that of GAPDH. Results shown are means ± standard deviations of (1/2)CT of target - CT of GAPDH of three experiments. B. AGR2 protein expression in the SNU-869 stable transfectants was detected by western blot analysis with beta -actin as a loading control. The result shown is a representative of three experiments. C. Viability of the SNU-869 stable transfectants was measured by the MTT assay. Results shown are means ± standard deviations of three experiments (ANOVA P = 0.008; post hoc analysis by Bonferroni testing: AGR2-2 vs. AGR2-N, P = 0.013). D. The effect of AGR2 overexpression on cell proliferation. Pulsed BrdU incorporation into the SNU-869 stable transfectants was measured by flow cytometry. Percentage of BrdU-positive cells was shown in mean ± standard deviation of four experiments (ANOVA P = 0.053; post hoc analysis by Bonferroni testing: AGR2-2 vs. AGR2-N, P = 0.027). Results shown are representatives of four experiments. E. The effect of AGR2 overexpression on tumor cell invasion of SNU-869 cells. The numbers of invading cells in the transwell invasion assay were determined for SNU-869 stable transfectants. Results shown are means ± standard deviations of three experiments (ANOVA P = 0.003; post hoc analysis by Bonferroni testing: AGR2-2 vs. AGR2-N, P = 0.026). Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27393_rabbit-polyclonal-ag-2-agr2-antibody-31020241613646.jpg)
![Western Blot: AG-2 Antibody (1C3) [H00010551-M03] - Analysis of AGR2 expression in MCF-7 (Cat # L046V1).](http://images.novusbio.com/fullsize/AG-2-Antibody-1C3-Western-Blot-H00010551-M03-img0004.jpg "Western Blot: AG-2 Antibody (1C3) [H00010551-M03] - Analysis of AGR2 expression in MCF-7 (Cat # L046V1).")
![Western Blot: AG-2 Antibody (1C3) [H00010551-M03] - Analysis of AGR2 expression in transfected 293T cell line by AGR2 monoclonal antibody (M03), clone 1C3. Lane 1: AGR2 transfected lysatE (20 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AG-2-Antibody-1C3-Western-Blot-H00010551-M03-img0005.jpg)
![ELISA: AG-2 Antibody (1C3) [H00010551-M03] - Detection limit for recombinant GST tagged AGR2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AG-2-Antibody-1C3-ELISA-H00010551-M03-img0003.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody [NB110-17780] - Immunohistochemical staining with NB110-17780, anti- AG-2 antibody, in formalin-fixed, paraffin-embedded normal colon tissue at 1:100 dilution.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-Paraffin-NB110-17780-img0005.jpg "Immunohistochemistry-Paraffin: AG-2 Antibody [NB110-17780] - Immunohistochemical staining with NB110-17780, anti- AG-2 antibody, in formalin-fixed, paraffin-embedded normal colon tissue at 1:100 dilution.")
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NB110-17780] - SATB1 immunohistochemistry staining. A, benign prostate hyperplasia. B, prostatic carcinoma with metastasis. C, prostatic carcinoma without metastasis. D, negative control. Magnification: 400 × . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23642278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-17780_rabbit-polyclonal-ag-2-agr2-antibody-31020241534351.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NB110-17780] - SATB1 immunohistochemistry staining. A, benign prostate hyperplasia. B, prostatic carcinoma with metastasis. C, prostatic carcinoma without metastasis. D, negative control. Magnification: 400 × . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23642278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-17780_rabbit-polyclonal-ag-2-agr2-antibody-310202415392510.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NB110-17780] - SATB1 immunohistochemistry staining. A, benign prostate hyperplasia. B, prostatic carcinoma with metastasis. C, prostatic carcinoma without metastasis. D, negative control. Magnification: 400 × . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23642278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-17780_rabbit-polyclonal-ag-2-agr2-antibody-310202415384145.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NB110-17780] - SATB1 immunohistochemistry staining. A, benign prostate hyperplasia. B, prostatic carcinoma with metastasis. C, prostatic carcinoma without metastasis. D, negative control. Magnification: 400 × . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23642278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-17780_rabbit-polyclonal-ag-2-agr2-antibody-31020241517528.jpg)
![Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Human stomach lysate.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Western-Blot-NBP1-05936-img0011.jpg "Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Human stomach lysate.")
![Immunocytochemistry/Immunofluorescence: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - AGR2 antibody was tested in MCF7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05936-img0012.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Representative immunohistological analysis of CD163 (scavenger receptor present in macrophages), AGR2, and TMED2 in non-inflamed colonic biopsies from healthy controls and patients with active or quiescent CD. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201810120) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0014.jpg)
![Western Blot: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Western blot analysis (left panel) and quantification (right panel) of AGR2 intracellular expression upon TMED2 overexpression. Data are representative of four independent experiments. Tubulin (TUB) was used as a loading control. The graph represents average signal normalized on reporter protein expression +/- SD. The Mann-Whitney statistical test was used. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31040128/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2 Antibody - BSA Free-Western Blot-NBP1-05936-img0015.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Detection of AGR2 in mouse prostate.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0009.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - Detection of AGR2 in mouse kidney.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0010.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NBP1-05936] - B. Analysis of AGR2 expression in the proximal colon of WT and TMED2 hypomorph mice. C.Semi-quantitative analysis of AGR2 expression in the proximal colon of WT (blank bars) and TMED2 hypomorph mice (black bars) (n = 6). The graph represents average IHC signal +/- SD. For statistical analyses, the Mann-Whitney test was used. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201810120) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody---BSA-Free-Immunohistochemistry-NBP1-05936-img0013.jpg)
![Western Blot: AG-2 Antibody (10E2) [NBP2-27175] - Analysis of human stomach in the A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using AGR2 antibody at 5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Western-Blot-NBP2-27175-img0019.jpg "Western Blot: AG-2 Antibody (10E2) [NBP2-27175] - Analysis of human stomach in the A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using AGR2 antibody at 5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - IHC-P detection of AGR2 protein in a human ovarian cancer section using 5 ug/ml concentration of AGR2 antibody. The representative image shows AGR2 immuno-reactivity in the developing ovarian cancer and the follicle cells of Graafian follicles. The cells of medulla region in the ovary did not show AGR2 positivity. [10X magnification]](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Immunohistochemistry-Paraffin-NBP2-27175-img0018.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Formalin-fixed, paraffin-embedded human breast stained with AGR2 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Immunohistochemistry-Paraffin-NBP2-27175-img0014.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Paraffin-embedded human small intestine tissue stained with AGR2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Immunohistochemistry-Paraffin-NBP2-27175-img0015.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Formalin-fixed, paraffin-embedded human spleen tissue stained with 5 ug/ml concentration of AGR2 antibody .](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Immunohistochemistry-Paraffin-NBP2-27175-img0016.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - IHC-P detection of AGR2 protein in a section of human bladded cancer using 5 ug/ml concentration of AGR2 antibody. The AGR2 immunoreactivity was observed in cellular cytoplasm and the inter-cellular spaces in cancerous areas of the sections. [40X magnification]](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Immunohistochemistry-Paraffin-NBP2-27175-img0017.jpg)
![Western Blot: AG-2 Antibody [NBP1-89212] - Analysis in human cell lines A-549 and HEK293 using anti-AGR2 antibody. Corresponding AGR2 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/AG-2-Antibody-Western-Blot-NBP1-89212-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: AG-2 Antibody [NBP1-89212] - Analysis in human cell lines A-549 and HEK293 using anti-AGR2 antibody. Corresponding AGR2 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AG-2 Antibody [NBP1-89212] - Staining of human cell line RT4 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89212-img0006.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89212-img0020.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Analysis in human stomach and skeletal muscle tissues. Corresponding AGR2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89212-img0016.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human stomach shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89212-img0017.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89212-img0018.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89212-img0019.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-05933] - Detection of AGR2 in mouse prostate.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-Paraffin-NBP1-05933-img0006.jpg "Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-05933] - Detection of AGR2 in mouse prostate.")
![Immunohistochemistry: AG-2 Antibody [NBP1-05933] - Detection of AGR2 in mouse kidney.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-NBP1-05933-img0005.jpg)
![Western Blot: AG-2/AGR2 Antibody (GT5812) [NBP3-13593] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AG-2/AGR2 antibody [GT5812] (NBP3-13593) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-GT5812-Western-Blot-NBP3-13593-img0001.jpg "Western Blot: AG-2/AGR2 Antibody (GT5812) [NBP3-13593] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AG-2/AGR2 antibody [GT5812] (NBP3-13593) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: AG-2/AGR2 Antibody (GT5812) [NBP3-13593] - AG-2/AGR2 antibody [GT5812] detects AG-2/AGR2 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AG-2/AGR2 stained by AG-2/AGR2 antibody [GT5812] (NBP3-13593) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-GT5812-Immunocytochemistry-Immunofluorescence-NBP3-13593-img0004.jpg)
![Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody (GT5812) [NBP3-13593] - AG-2/AGR2 antibody [GT5812] detects secreted AG-2/AGR2 protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. AG-2/AGR2 stained by AG-2/AGR2 antibody [GT5812] (NBP3-13593) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-GT5812-Immunohistochemistry-Paraffin-NBP3-13593-img0003.jpg)
![Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody (GT5812) [NBP3-13593] - AG-2/AGR2 antibody [GT5812] detects secreted AG-2/AGR2 protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. AG-2/AGR2 stained by AG-2/AGR2 antibody [GT5812] (NBP3-13593) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-GT5812-Immunohistochemistry-Paraffin-NBP3-13593-img0002.jpg)
![Anterior Gradient 2 antibody [GT5812] detects Anterior Gradient 2 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (NBP3-13593) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13593_mouse-ag-2-agr2-mab-gt5812-immunocytochemistry-immunofluorescence-2611202518413924.jpg)
![Western Blot: AG-2/AGR2 Antibody (HL1024) [NBP3-13684] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AG-2/AGR2 antibody [HL1024] (NBP3-13684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-HL1024-Western-Blot-NBP3-13684-img0003.jpg "Western Blot: AG-2/AGR2 Antibody (HL1024) [NBP3-13684] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AG-2/AGR2 antibody [HL1024] (NBP3-13684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody (HL1024) [NBP3-13684] - AG-2/AGR2 antibody [HL1024] detects AG-2/AGR2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.AG-2/AGR2 stained by AG-2/AGR2 antibody [HL1024] (NBP3-13684) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13684_rabbit-ag-2-agr2-mab-hl1024-9102024195723.jpg)
![Immunocytochemistry/ Immunofluorescence: AG-2/AGR2 Antibody (HL1024) [NBP3-13684] - AG-2/AGR2 antibody [HL1024] detects AG-2/AGR2 protein at cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: AG-2/AGR2 stained by AG-2/AGR2 antibody [HL1024] (NBP3-13684) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13684_rabbit-ag-2-agr2-mab-hl1024-910202419593580.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: AG-2/AGR2 Antibody (HL1024) [NBP3-13684] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Anterior Gradient 2 antibody [HL1024] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13684_rabbit-ag-2-agr2-mab-hl1024-1610202419405753.jpg)
![Western Blot: AG-2/AGR2 Antibody (S04-1H6) [NBP3-19534] - Western blot detection of AG-2/AGR2 in K562, C6 cell lysates using NBP3-19534 (1:1000 diluted). Predicted band size: 20kDa. Observed band size: 18kDa.](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-S04-1H6-Western-Blot-NBP3-19534-img0003.jpg "Western Blot: AG-2/AGR2 Antibody (S04-1H6) [NBP3-19534] - Western blot detection of AG-2/AGR2 in K562, C6 cell lysates using NBP3-19534 (1:1000 diluted). Predicted band size: 20kDa. Observed band size: 18kDa.")
![Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody (S04-1H6) [NBP3-19534] - Immunohistochemistry of AG-2/AGR2 in paraffin-embedded colon using NBP3-19534 at dilution 1/100](http://images.novusbio.com/fullsize/AG-2-AGR2-Antibody-S04-1H6-Immunohistochemistry-Paraffin-NBP3-19534-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AG-2/AGR2 Antibody (S04-1H6) [NBP3-19534] - Immunofluorescence of AG-2/AGR2 (green) in MCF-7 using NBP3-19534 at dilution 1/2, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19534_rabbit-ag-2-agr2-mab-s04-1h6-113202515145438.jpg)