Nebulin Antibody - BSA Free

Immunohistochemistry-Paraffin: Nebulin Antibody [NBP1-87752] - Analysis in human skeletal muscle and cerebral cortex tissues using NBP1-87752 antibody. Corresponding NEB RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Nebulin Antibody [NBP1-87752] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Immunohistochemistry-Paraffin: Nebulin Antibody [NBP1-87752] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: Nebulin Antibody [NBP1-87752] - Staining of human duodenum shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Nebulin Antibody [NBP1-87752] - Staining of human cerebral cortex shows no positivity in neurons as expected.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Nebulin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Nebulin Antibody - BSA Free (NBP1-87752) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DTPDMLRAKNATQILNEKEYKRDLELEVKGRGLNAMANETPDFMRARNATDIASQIKYKQSAEMEKANFTSVVDTPEIIHAQQVKNLSSQKKYKEDAEKSMSYYETVLDTPEIQRVRENQKNFSLLQYQCDL
• Predicted Species: Mouse (99%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Nebulin Protein (NBP1-87752PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Nebulin Antibody - BSA Free

• DKFZp686C1456
• FLJ11505
• FLJ36536
• FLJ39568
• FLJ39584
• NEB177D
• nebulin
• NEM2

Background

Nebulin is a high molecular weight protein (500-800 kD), localized at the N2-line of skeletal muscle myofibrils. It exhibits an elastic stretch dependence, as it maintains the same proportional distance between the z-line and the m-line as the sarcomere is stretched.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEB