Acidic Calponin Antibody - BSA Free

Western Blot: Acidic Calponin Antibody [NBP2-15242] - Sample (30 ug of whole cell lysate) A: 293T B: HeLa C: HepG2 10% SDS PAGE; antibody diluted at 1:5000.

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-15242] - Paraffin-embedded rat ovary. calponin 3 antibody diluted at 1:500.

Western Blot: Acidic Calponin Antibody [NBP2-15242] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE; antibody diluted at 1:1000.

Western Blot: Acidic Calponin Antibody [NBP2-15242] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Acidic Calponin antibody (NBP2-15242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Pm, RM, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Acidic Calponin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Acidic Calponin Antibody - BSA Free (NBP2-15242) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Acidic Calponin. The exact sequence is proprietary.
• Predicted Species: Bovine (100%), Rhesus Macaque (100%), Xenopus (90%), Primate (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CNN3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (99%), Xenopus laevis (90%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Acidic Calponin Antibody - BSA Free

• calponin 3, acidic
• Calponin, acidic isoform
• calponin-3
• dJ639P13.2.2 (acidic calponin 3)

Background

This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CNN3
• Uniprot:
  • Q15417(Human)