Troponin I Type 3 (cardiac) Antibody (19C7cc) - BSA Free

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (19C7cc) [NB110-2546] - Mouse cardiac tissue stained with Troponin I Type 3 antibody. IHC-P image submitted by a verifed customer review.

Flow Cytometry: Troponin I Type 3 (cardiac) Antibody (19C7) [NB110-2546] - FACS analysis of Troponin I Type 3 in differentiated ESC cultures using anti-Troponin I Type 3 (cardiac) antibody. Image from verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IHC, IP, AP
• Clone: 19C7cc
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Troponin I Type 3 (cardiac) Antibody (19C7cc) - BSA Free Summary

• Description: Novus Biologicals Mouse Troponin I Type 3 (cardiac) Antibody (19C7cc) - BSA Free (NB110-2546) is a monoclonal antibody validated for use in IHC, WB, Flow and IP. Anti-Troponin I Type 3 (cardiac) Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnI.
• Epitope: a.a.r. 41-49
• Specificity: Troponin I Type 3 (cardiac). No cross-reacticity with skeletal muscle TnI.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNI3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoaffinity Purification
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Recommended pairs for sandwich immunoassay (capture-detection): NB110-2546 - NB100-73082, NB110-2546 - NB100-73087, NB100-73085 - NB110-2546, or NB110-8453 - NB110-2546.
• Control:

  Troponin T type 1 (slow skeletal) Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Troponin I Type 3 (cardiac) Antibody (19C7cc) - BSA Free

• Cardiac troponin I
• CMD1FF
• CMD2A
• CMH7MGC116817
• CTnI
• familial hypertrophic cardiomyopathy 7
• RCM1
• TNNC1
• TNNC1troponin I, cardiac
• TNNI3,
• troponin I type 3 (cardiac)
• troponin I, cardiac muscle
• Troponin I/TNNI3

Background

Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Troponin I Type 3 (cardiac) Antibody (NB110-2546)(6)

Publications using NB110-2546

• Munasinghe P, Saw E, Reily-Bell M et al. Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes Heliyon 2023-06-01 [PMID: 37426799] (IHC-Fr, Mouse)
• Bahnan W, Happonen L, Khakzad H et al. A human monoclonal antibody bivalently binding two different epitopes in streptococcal M protein mediates immune function EMBO molecular medicine 2022-12-12 [PMID: 36507602] (IHC-P, Human)
• Ghazizadeh Z, Zhu J, Fattahi F, Tang A et Al. A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells iScience 2022-04-18 [PMID: 35434558]
• Pacheco-Leyva I, Matias AC, Oliveira DV et al. CITED2 Cooperates with ISL1 and Promotes Cardiac Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2016-12-13 [PMID: 27818139] (FLOW, Mouse)
• Selimoglu SM, Kasap M, Akpinar G, Karadenizli A Overcoming difficulties on synthesis of cardiac troponin-I. Prep Biochem Biotechnol 2016-04-12 [PMID: 27070210]
• Munasinghe PE Activation of the cardiac non-neuronal cholinergic system prevents type-1 diabetes-induced cardiovascular dysfunction Thesis 2020-01-01 (IF/IHC)

Reviews for Troponin I Type 3 (cardiac) Antibody (NB110-2546) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 08/30/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Cardiac tissue
• Species: Mouse
• Lot: 19C7cc

reviewed by: Ivette Leyva Flow Mouse 10/09/2014

Summary

• Application: Flow Cytometry
• Sample Tested: differentiated ESC cultures
• Species: Mouse
• Lot: 13/05-T21-C7

FAQs for Troponin I Type 3 (cardiac) Antibody (NB110-2546). (Showing 1 - 2 of 2 FAQs).

1. Would you please help suggest antibody pairs for detecting Human Creatine Kinase MB,.Myoglobin,.C-reactive protein, and Troponin I, as well as their positive controls? So far, I can only find NB120-8345 and NB600-1291 for Myoglobin. There seems no Creatine Kinase MB antibody available from Novus. I also found two pairs suggested for Troponin I: NB110-2546(Coating)- NB100-73082(Conjugate); NB100-73087(Coating) - NB110-2546(Conjugate). Would you please help confirm? How about CRP? May we also ask if NB810-73233 can be used as the antigen in ELISA of CRP?
   • For Myoglobin The recommended antibodies are NB120-8345 and NB600-1291 as you mentioned. For Troponin I: As you mentioned: NB110-2546(Coating)- NB100-73082(Conjugate) and NB100-73087(Coating) - NB110-2546(Conjugate). We do not have any matched pairs for Creatine KinasE MB. We do not have any match pairs for CRP as we have just not tested these in house in ELISA. The customer could use our CRP antibodies in a direct ELISA. We have not had any one use this as an antigen as of yet however if your customer is willing to test this as innovators they are more than welcome. See full details of our Innovators Reward Program here or contact us at innovators@novusbio.com for more information.
2. Is the antibody cardiac specific? If no, what is the percentage that it is cardiac specific?
   • It is expected to be cardiac specific and to not cross-react with skeletal troponin I.

Bioinformatics

• Gene Symbol: TNNI3
• Entrez:
  • 7137(Human)
  • 21955(Mouse)
  • 171409(Rat)
• Uniprot:
  • P19429()