Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4)

Western Blot: Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4) [NBP3-33573] - Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-CoA Carboxylase Rabbit mAb at 1:7000 dilution. NIH/3T3 cells were treated by oligomycin (0. 5 uM) at 37C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

• Reactivity: Mu, Rt
• Applications: WB, ELISA
• Clone: 3P2E4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4) Summary

• Description: Novus Biologicals Rabbit Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4) (NBP3-33573) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around S79 of human Acetyl-CoA Carboxylase
• Modification: p Ser79
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACACA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:2000 - 1:8000
• Theoretical MW: 265 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4)

• ACAC
• Acac1
• ACACAD
• ACC
• ACC1
• ACCA
• ACC-alpha
• ACCalphaACACalpha
• acetyl-CoA carboxylase 1
• acetyl-Coenzyme A carboxylase alpha
• hACC1

Background

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACACA