Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [ac Ser79] Antibody [NBP3-05407] - Western blot analysis of extracts of 293T cells, using Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-05407) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free [Acetyl-CoA Carboxylase alpha/ACACA] - Western blot analysis of lysates from RAW264.7 cells, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit pAb at 1:700 dilution. RAW264. 7 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free [Acetyl-CoA Carboxylase alpha/ACACA] - Western blot analysis of lysates from A-431 cells, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit pAb at 1:700 dilution. A-431 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free (NBP3-05407) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around S79 of human Acetyl-CoA Carboxylase alpha/ACACA (NP_942133.1). SMSGL
• Modification: p Ser79
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACACA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free

• ACACA
• ACACacetyl-CoA carboxylase 1
• ACC1
• ACC1ACC
• ACCA
• ACC-alpha
• AcetylCoA Carboxylase alpha
• Acetyl-CoA Carboxylase alpha
• acetyl-Coenzyme A carboxylase alpha
• EC 6.4.1.2

Background

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ACACA