Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (RM270)

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (RM270) [NBP2-61473] - Western Blot of A431 cell lysates, nontreated (-) or treated (+) with Lambda Protein Phosphatase (lPP), using NBP2-61473 at a 1:1000 dilution.

Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (RM270) [NBP2-61473] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61473 at a 1:5000 dilution

• Reactivity: Hu
• Applications: WB, IHC
• Clone: RM270
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (RM270) Summary

• Description: Novus Biologicals Rabbit Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (RM270) (NBP2-61473) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: A phospho-peptide corresponding to Human Acetyl-CoA Carboxylase alpha/ACACA [p Ser79]
• Modification: p Ser79
• Specificity: This antibody reacts to human Acetyl-CoA Carboxylase alpha/ACACA only when phosphorylated at Ser79. There is no cross-reactivity to Acetyl-CoA Carboxylase alpha/ACACA without phosphorylation at Ser79.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACACA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000-1:2000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000 - 1:5000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (RM270)

• ACACA
• ACACacetyl-CoA carboxylase 1
• ACC1
• ACC1ACC
• ACCA
• ACC-alpha
• AcetylCoA Carboxylase alpha
• Acetyl-CoA Carboxylase alpha
• acetyl-Coenzyme A carboxylase alpha
• EC 6.4.1.2

Background

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACACA