ACE/CD143 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, Flow, IHC, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ACE/CD143 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse ACE/CD143
  Leu35-Gln1264
  Accession # P09470
• Specificity: Detects mouse ACE/CD143 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant mouse ACE-2 and recombinant human ACE is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACE
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse ACE/CD143 Antibody (Catalog # MAB15132)
  Standard: Recombinant Mouse ACE/CD143 Somatic Form (Catalog # 1513-ZN)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE/CD143 Antibody [Biotin]

• ACE
• ACE1angiotensin converting enzyme, somatic isoform
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
• carboxycathepsin
• CD143 antigen
• CD143
• DCP
• DCP1
• DCP1angiotensin-converting enzyme
• dipeptidyl carboxypeptidase 1
• Dipeptidyl carboxypeptidase I
• EC 3.2.1.-
• EC 3.4.15.1
• Kininase II
• MGC26566
• MVCD3
• peptidase P
• testicular ECA

Background

ACE (also known as peptidyl-dipetidase A) is a zinc metallopeptidase important for blood pressure control and water and salt metabolism (1). It cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II and inactivates bradykinin by the sequential removal of two C-terminal dipeptides. In addition to the two physiological substrates, ACE cleaves C-terminal dipeptides from various oligopeptides with a free C-terminus. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. For example, ACE degrades Alzheimer amyloid beta -peptide (A beta ), retards A beta aggregation, deposition, fibril formation, and inhibits cytotoxicity (2).

ACE is a type I membrane protein and exists in two isoforms (1). Somatic ACE, found in endothelial, epithelial and neuronal cells, comprises two highly similar catalytic domains called N- and C-domains. Germinal ACE, found exclusively in the testes, comprises a single catalytic domain identical to the C-domain of somatic ACE except for an N-terminal 67 residue germinal ACE-specific sequence. Physiological functions of the two tissue-specific isozymes are not interchangeable (3). For example, sperm-specific expression of the germinal ACE, not the somatic ACE, in ACE knockout male mice restored fertility.

Soluble ACE is present in many biological fluids, such as serum, seminal fluid, amniotic fluid and cerebrospinal fluid (1). The soluble ACE is derived from the membrane forms by actions of secretases or sheddases. The identities of the secretases have not been revealed, although they belong to the family of zinc metallopeptidases (4, 5).

1. Corvol, P. et al. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.) p. 332, Academic Press, San Diego.
2. Hu, J. et al. (2001) J. Biol. Chem. 276:47863.
3. Kessler, S.P. et al. (2000) J. Biol. Chem. 275:26259.
4. Eyries, M. et al. (2001) J. Biol. Chem. 276:5525.
5. Alfalah, M. et al. (2001) J. Biol. Chem. 276:21105.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE/CD143 Antibody (BAF1513)(3)

Publications using BAF1513

• Rodolfo Mattar Rosa, Juliana Almada Colucci, Rodrigo Yokota, Roseli Peres Moreira, Danielle Sanches Aragão, Amanda Aparecida Ribeiro et al. Alternative pathways for angiotensin II production as an important determinant of kidney damage in endotoxemia American Journal of Physiology-Renal Physiology 2016-09-01 [PMID: 27252489] (Western Blot, Rat)
• Rodolfo Mattar Rosa, Juliana Almada Colucci, Rodrigo Yokota, Roseli Peres Moreira, Danielle Sanches Aragão, Amanda Aparecida Ribeiro et al. Alternative pathways for angiotensin II production as an important determinant of kidney damage in endotoxemia American Journal of Physiology-Renal Physiology 9/1/2016 [PMID: 27252489]
• Spencer AU, Yang H, Haxhija EQ, Wildhaber BE, Greenson JK, Teitelbaum DH Reduced severity of a mouse colitis model with angiotensin converting enzyme inhibition. Dig. Dis. Sci., 2007-03-07;52(4):1060-70. 2007-03-07 [PMID: 17342403] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ACE
• Uniprot:
  • P09470()