ACE-2 Antibody [Unconjugated]

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE‑2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ACE‑2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 141 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

ACE‑2 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Simple Western, Flow, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ACE-2 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse ACE-2
  Gln18-Thr740
  Accession # Q8R0I0
• Specificity: Detects mouse ACE-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat ACE‑2 and 10% cross-reactivity with recombinant human ACE-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACE2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Unconjugated]

• ACE2
• ACE-2
• ACEH
• ACEHangiotensin I converting enzyme 2
• ACE-related carboxypeptidase
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• angiotensin-converting enzyme 2
• Angiotensin-converting enzyme homolog
• DKFZp434A014
• EC 3.4.17
• EC 3.4.17.23
• Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).

1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
2. Crackower, M.A. et al. (2002) Nature 417:822.
3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
4. Kuba, K. et al. (2005) Nature Med. 11:875.
5. Ima, Y. et al. (2005) Nature 436:112.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE-2 Antibody (AF3437)(4)

Publications using AF3437

• Bilinska K, Jakubowska P, Von Bartheld C, Butowt R Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in Cells of the Olfactory Epithelium: Identification of Cell Types and Trends with Age. ACS Chem Neurosci, 0;11(11):1555-1562. 0 [PMID: 32379417] (Western Blot, Mouse)
• J Jando, SMR Camargo, B Herzog, F Verrey Expression and regulation of the neutral amino acid transporter B0AT1 in rat small intestine PLoS ONE, 2017;12(9):e0184845. 2017 [PMID: 28915252] (Western Blot, Rat)
• Hashimoto T, Perlot T, Rehman A ACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation. Nature, 2012;487(7408):477-81. 2012 [PMID: 22837003] (Western Blot, Mouse)
• Singer D, Camargo SM, Huggel K, Romeo E, Danilczyk U, Kuba K, Chesnov S, Caron MG, Penninger JM, Verrey F Orphan transporter SLC6A18 is renal neutral amino acid transporter B0AT3. J. Biol. Chem., 2009;284(30):19953-60. 2009 [PMID: 19478081] (Western Blot, Mouse)

Review for ACE-2 Antibody (AF3437) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22777933
• Species: Mouse

Blocking

• Blocking Details: See PMID 22777933

Details

• Detection Notes: See PMID 22777933

Comments

• Comments: Published: See PMID 22777933Independent review from antibodyresource.comIn Western blot gives a clean signal; does not cross-react with ACE;

Bioinformatics

• Gene Symbol: ACE2
• Uniprot:
  • Q8R0I0()