ABCA1 Antibody (3A1.891.3)

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Biological Strategies: Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Western blot analysis of lipid transporters in macrophages treated with or without oxLDL using antibodies against ABCA1 (NB400-164), ...read more
Immunohistochemistry-Paraffin: ABCA1 Antibody (3A1.891.3) [NB400-164] - Analysis of FFPE mouse liver using ABCA1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat ...read more
Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Detection of ABCA1 using NB400-164 at various dilutions in ABCA1 transfected HeLa lysates.
Biological Strategies: Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Analysis of ABCA1 in (A) T09 treated RAW 264.7 cells and (B) untreated RAW 264.7 cells.

Product Details

Summary
Reactivity Mu, Hu(-)Species Glossary
Applications WB, IHC, IHC-P
Clone
3A1.891.3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

ABCA1 Antibody (3A1.891.3) Summary

Immunogen
A1-N3 peptide (within residues 200-250) of mouse ABCA1 Antibody (3A1.891.3) covalently linked to chicken egg albumin by means of glutaraldehyde. [Uniprot: P41233]
Localization
Cell Membrane
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Gene
ABCA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Western Blot 1:1000-1:3000
Application Notes
In Western blot, a band is observed at ~250 kDa. ABCA1 is known to aggregate. Do not boil samples and use a reducing buffer. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 19718435).
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Raw 264.7 Whole Cell - T0901317 treated Assay Kit
THP-1 TO901317 Treated / Untreated Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB400-164 in the following applications:

Publications
Read Publications using
NB400-164 in the following applications:

  • 1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for ABCA1 Antibody (3A1.891.3)

  • ABC1
  • ABC-1
  • ABC1ATP-binding cassette transporter A1
  • ABCA1
  • ATP-binding cassette 1
  • ATP-binding cassette transporter 1
  • ATP-binding cassette, sub-family A (ABC1), member 1
  • CERP
  • Cholesterol efflux regulatory protein
  • EC 2.7.7.8
  • EC 2.8.1.8
  • EC 3.6.3
  • EC 3.6.3.41
  • FLJ14958
  • HDLDT1
  • membrane-bound
  • MGC164864
  • MGC165011
  • TGD
  • TGDATP-binding cassette sub-family A member 1

Background

ATP-binding cassette transporter A1 (ABCA1) is a cAMP-dependent and sulfonylurea-sensitive anion transporter belonging to the ATP-binding cassette family. ABCA1 is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux and high-density lipoproteins (HDL) metabolism (1). In the brain, ABCA1 transports cholesterol to apoE, the major CNS apolipoprotein, where it influences motor function and synaptic morphology.

ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).

References

1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617

2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522

3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ABCA1 Antibody (NB400-164)(9)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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(1)
WB
(5)
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(6)
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Showing Publications 1 - 9 of 9.
Publications using NB400-164 Applications Species
Ishimaru K, Yoshioka K, Kano K, et al. Sphingosine kinase-2 prevents macrophage cholesterol accumulation and atherosclerosis by stimulating autophagic lipid degradation Sci Rep Dec 4 2019 [PMID: 31797978] (WB, Mouse) WB Mouse
Quiroz A, Molina P, Santander N et al. Ovarian Cholesterol Efflux: ABC Transporters and Follicular Fluid HDL Regulate Cholesterol Content in Mouse Oocytes Biol. Reprod. Aug 19 2019 [PMID: 31423535]
Xian X, Ding Y, Dieckmann M et al. LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis Elife. 2017 Nov 16 [PMID: 29144234] (WB, Mouse) WB Mouse
Sultana A, Cochran BJ, Tabet F et al. Inhibition of inflammatory signaling pathways in 3T3-L1 adipocytes by apolipoprotein A-I. FASEB J. 2016 Mar 10 [PMID: 26965683] (WB, Mouse) WB Mouse
Do TM, Dodacki A, Alata W et al. Age-Dependent Regulation of the Blood-Brain Barrier Influx/Efflux Equilibrium of Amyloid-beta Peptide in a Mouse Model of Alzheimer's Disease (3xTg-AD). J Alzheimers Dis 2015 [PMID: 26484906] (Mouse) Mouse
Do TM, Noel-Hudson MS, Ribes S et al. ABCG2- and ABCG4-mediated efflux of amyloid-beta peptide 1-40 at the mouse blood-brain barrier J Alzheimers Dis 2012 [PMID: 22391220]
Do TM, Ouellet M, Calon F et al. Direct evidence of abca1-mediated efflux of cholesterol at the mouse blood-brain barrier. Mol Cell Biochem. 2011 Jun 10. [PMID: 21660464] (WB, Mouse) WB Mouse
Wang F, Okamoto Y, Inoki I et al. Sphingosine-1-phosphate receptor-2 deficiency leads to inhibition of macrophage proinflammatory activities and atherosclerosis in apoE-deficient mice. J Clin Invest. 120(11):3979-95. doi: 10.1172/JCI42315. 2010 Nov 1. [PMID: 20978351]
Zhou L, Choi HY, Li WP et al. LRP1 controls cPLA2 phosphorylation, ABCA1 expression and cellular cholesterol export. PLoS One. 2009 Aug 31 [PMID: 19718435] (WB, IHC-P, Mouse) WB, IHC-P Mouse

Review for ABCA1 Antibody (NB400-164) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB400-164:
Filter by Applications
Western Blot
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
Western Blot Mouse 10/25/2011
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Summary

ApplicationWestern Blot
Sample TestedBrain microvessels total proteins, Sample Amount: 20 micrograms
SpeciesMouse

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Video Protocols

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FAQs for ABCA1 Antibody (NB400-164). (Showing 1 - 1 of 1 FAQs).

  1. For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?
    • For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.

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Secondary Antibodies

 

Isotype Controls

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Blogs on ABCA1. Showing 1-10 of 14 blog posts - Show all blog posts.

HIV-associated neurocognitive disorders involve extracellular Nef-induced modification of lipid rafts and redistribution of Alzheimer’s disease-related proteins
Jamshed Arslan, Pharm D, PhD Cholesterol is an essential part of animal cell membranes. Cholesterol-rich lipid rafts maintain the fluidity and protein trafficking of plasma membranes. Cellular ABCA1 protein moves cho...  Read full blog post.

Phagocytes in Multiple Sclerosis: Myelin uptake leads to oxysterol-induced activation of liver X receptors, LXRs
By Jamshed Arslan Pharm.D., PhD.Myelin is a cholesterol-rich layer around nerves. Damage to this protective layer and the consequent slowing down of nerve impulses are hallmarks of multiple sclerosis (MS) and other ...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

ABCG8: Cholesterol's Fate
The ATP-binding cassette (ABC) transporter genes are key gatekeeper molecules that regulate the amount of dietary cholesterol retained by the body. They are a multifamily comprised of cAMP-dependent anion transporter cell membrane proteins that monito...  Read full blog post.

ABCA1
ABCA1 is a key gatekeeper influencing intracellular cholesterol transport, and is an important member of a multifamily of cAMP-dependent anion transporter cell membrane proteins that regulate reverse cholesterol efflux from cells in peripheral tissues...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. ...  Read full blog post.

SREBP2: From Cholesterol Homeostasis to Cancer Invasion
Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP...  Read full blog post.

LXR Alpha, ABCA1 and Cholesterol Homeostasis
LXR Alpha, also known as Liver X receptor Alpha is a 50KDa protein that belongs to the nuclear hormone receptor family located in the nucleus. It is specifically expressed in the liver, kidney and intestine; however it has also been found in the splee...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.
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Verified Customer
10/25/2011
Application: Western Blot
Species: Mouse

Bioinformatics

Gene Symbol ABCA1
Entrez