ABCA1 Antibody (3A1.891.3)

Images

 
Immunohistochemistry-Paraffin: ABCA1 Antibody (3A1.891.3) [NB400-164] - IHC analysis of formalin fixed paraffin-embedded (FFPE) mouse liver using ABCA1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The ...read more
Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - WB analysis of ABCA1 in A. T09 treated RAW 264.7 cells and B. untreated RAW 264.7 cells.
Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Detection of ABCA1 using NB 400-164 at various dilutions in ABCA1 transfected HeLa lysates.

Product Details

Summary
Reactivity Mu, Hu(-)Species Glossary
Applications WB, IHC, IHC-P
Clone
3A1.891.3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
   

Biological Strategies

     

Order Details

ABCA1 Antibody (3A1.891.3) Summary

Immunogen
A1-N3 peptide (within residues 200-250) of mouse ABCA1 covalently linked to chicken egg albumin by means of glutaraldehyde. [UniProt# P41233]
Localization
Cell Membrane
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Gene
ABCA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
Application Notes
This ABCA1 antibody is useful for Western blot, where a band is observed at ~250 kDa. ABCA1 is known to aggregate. Do not boil samples and use a reducing buffer. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 19718435) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Raw 264.7 Whole Cell - T0901317 treated Kit (NBP1-30159)
Reviewed Applications
Read 1 Review rated 5
using
NB400-164 in the following applications:

Publications
Read Publications using
NB400-164 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Mouse. Does not react with human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for ABCA1 Antibody (3A1.891.3)

  • ABC1
  • ABC-1
  • ABC1ATP-binding cassette transporter A1
  • ABCA1
  • ATP-binding cassette 1
  • ATP-binding cassette transporter 1
  • ATP-binding cassette, sub-family A (ABC1), member 1
  • CERP
  • Cholesterol efflux regulatory protein
  • EC 2.7.7.8
  • EC 2.8.1.8
  • EC 3.6.3
  • EC 3.6.3.41
  • FLJ14958
  • HDLDT1
  • membrane-bound
  • MGC164864
  • MGC165011
  • TGD
  • TGDATP-binding cassette sub-family A member 1

Background

ABCA1 (ATP-binding cassette transporter A1), ATP-binding cassette transporter family member, is a cAMP-dependent and sulfonylurea-sensitive anion transporter which is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux from macrophages and act as a key gatekeeper influencing intracellular cholesterol transport. ABCA1 is widely expressed with highest expression levels found in macrophages and is induced by bacterial LPS which regulates its expression through LXR (liver X receptor) -independent mechanism. ABCA1 interacts with MEGF10 and its expression is repressed by ZNF202. ABCA1 phosphorylation at Ser-2054 site regulates the phospholipid efflux and DHHC8 mediated palmitoylation of ABCA1 is essential for its membrane localization. ABCA1 expressed in brain neurons/glia mediates cholesterol transport to apoE which is the major CNS apolipoprotein, and it influences the motor function and synaptic morphology also. Defects in ABCA1 have been linked to HDLD1 (high density lipoprotein deficiency type 1; also known as analphalipoproteinemia, Tangier disease or TGD) and HDLD2 (high density lipoprotein deficiency type 2; also known as familial hypoalphalipoproteinemia or FHA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-164
Species: Mu, Hu(-)
Applications: WB, IHC, IHC-P

Publications for ABCA1 Antibody (NB400-164)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(3)
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(4)
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Review for ABCA1 Antibody (NB400-164) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB400-164:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
DO Tuan Minh
WB Mouse 10/25/2011
View

Summary

ApplicationWestern Blot
Sample TestedBrain microvessels total proteins, Sample Amount: 20 micrograms
SpeciesMouse
CommentsWe have obtained a specific band of Abca1 at ~ 250 kDa when using this antibody for studying Abca1 expression at the mouse blood-brain barrier. Quantity of protein loading was 20 micrograms.

Blocking

Blocking DetailsBlocking Buffer: 10 % Milk in TTBS, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1500, Primary Ab Incubation Dilution Buffer: TTBS, Incubation Time: 2hrs, Incubation Temp: RT

Secondary Antibody

Secondary DescriptionSecondary Ab: Rabbit anti-rat secondary antibody, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesSpecific Bands: 250kDa

Comments

CommentsWe have obtained a specific band of Abca1 at ~ 250 kDa when using this antibody for studying Abca1 expression at the mouse blood-brain barrier. Quantity of protein loading was 20 micrograms.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ABCA1 Antibody (NB400-164). (Showing 1 - of FAQs).

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ABCA1 Antibody (NB400-164)

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Blogs on ABCA1. Showing 1-10 of 12 blog posts - Show all blog posts.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

ABCG8: Cholesterol's Fate
The ATP-binding cassette (ABC) transporter genes are key gatekeeper molecules that regulate the amount of dietary cholesterol retained by the body. They are a multifamily comprised of cAMP-dependent anion transporter cell membrane proteins that monito...  Read full blog post.

ABCA1
ABCA1 is a key gatekeeper influencing intracellular cholesterol transport, and is an important member of a multifamily of cAMP-dependent anion transporter cell membrane proteins that regulate reverse cholesterol efflux from cells in peripheral tissues...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

SREBP2: From Cholesterol Homeostasis to Cancer Invasion
Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP...  Read full blog post.

LXR Alpha, ABCA1 and Cholesterol Homeostasis
LXR Alpha, also known as Liver X receptor Alpha is a 50KDa protein that belongs to the nuclear hormone receptor family located in the nucleus. It is specifically expressed in the liver, kidney and intestine; however it has also been found in the splee...  Read full blog post.

The ABCA1 Antibody and ABC Transporter Research
The ABC (ATP-binding cassette) transporters are a superfamily of transmembrane proteins which play an essential role in membrane transport in both prokaryotes and eukaryotes. In humans, ABC transporters have been implicated in many conditions. For exa...  Read full blog post.

Exploring how ABCG8 Affects Heart Disease
The high prevalence of atherosclerosis in developed nations is not breaking news; it has been well established that heart disease is the leading cause of death in the United States, and it presents a major socioeconomic burden. Investigators across th...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

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Recent Reviews

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1
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DO Tuan Minh
10/25/2011
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ABCA1
Entrez