Home » 5-HT4 » 5HT4 Receptor Research Products » 5-HT4 Antibody

5-HT4 Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LYVE1 in 1% milk/TBST. Reactivity was detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunocytochemistry/ Immunofluorescence: 5-HT4 Antibody [NBP1-78403] - Staining of 5HT4 receptor in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.09 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-78403AF350
Alexa Fluor 405
NBP1-78403AF405
Alexa Fluor 488
NBP1-78403AF488
Alexa Fluor 532
NBP1-78403AF532
Alexa Fluor 594
NBP1-78403AF594
Alexa Fluor 647
NBP1-78403AF647
Alexa Fluor 700
NBP1-78403AF700
Alexa Fluor 750
NBP1-78403AF750
Biotin
NBP1-78403B
DyLight 350
NBP1-78403UV
DyLight 405
NBP1-78403V
DyLight 488
NBP1-78403G
DyLight 550
NBP1-78403R
DyLight 594
NBP1-78403DL594
DyLight 650
NBP1-78403C
DyLight 680
NBP1-78403FR
DyLight 755
NBP1-78403IR
Janelia Fluor 549
NBP1-78403JF549
Janelia Fluor 646
NBP1-78403JF646
100% Guarantee on every product

5-HT4 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human 5HT4 Receptor protein (between residues 350-388) [UniProt Q13639, isoform 1]
Localization
Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HTR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This 5HT4 Receptor antibody is useful for Immunocytochemistry/Immunofluorescence where cytoplasmic staining is observed in HeLa cells.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.09 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 5-HT4 Antibody

  • 5HT4
  • 5-HT4
  • 5-HT-4
  • 5-HT4R
  • 5-hydroxytryptamine (serotonin) receptor 4,5-hydroxytryptamine receptor 4,5-HT45-hydroxytryptamine4 receptor
  • cardiac 5-HT4 receptor
  • HTR4
  • Serotonin receptor 4

Background

The 5HT4 receptor (5-HT4R) is a member of the serotonin receptors. In response to serotonin (5HT) these G protein coupled receptors cause an increase in cAMP production. It is a glycosylated, transmembrane protein found in the central and peripheral nervous system. Multiple transcripts with variant C-terminal sequnces have been described. The significance of SNP variants of 5HT4 receptors in mental diseases is a controversial topic (PMID:19328558, 19892407), while other SNP variants have been associated with asthma.( PMID:21382128). Additional research show that the early presence of these receptors in the dorsomedial nuclei of prenatal hypothalamus may indicate a crucial role in fetal development. (PMID:20538346)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

5-HT2A Antibody
NBP2-26091
Immunocytochemistry/Immunofluorescence: 5-HT2A Antibody [NBP2-26091] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: 5-HT2A Antibody [NBP2-26091] - Staining of paraffin embedded Human Cerebellum. Antibody at 2 ug/mL. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: 5-HT2A Antibody [NBP2-26091] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
5-HT1A Antibody
NBP2-21590
Western Blot: 5-HT1A Antibody [NBP2-21590] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: 5-HT1A Antibody [NBP2-21590] - DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: 5-HT1A Antibody [NBP2-21590] - Frozen Sectioned adult mouse hippocampus. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:250.Blue: Fluoroshield with DAPI.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
GR/NR3C1 Antibody
(BuGR2)
NB300-731
Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
(3 Reviews)
(11 Publications)
Species: Hu, Mu, Rt, Gp, Rb, Sh, Ye
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, B/N
Glutathione
Reductase Antibody
NBP2-16684
Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Glutathione Reductase Antibody [NBP2-16684] - Paraffin-embedded rat brain. Glutathione reductase antibody [N2C2], Internal diluted at 1:500.
Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
5-HT7 Antibody
NB100-56352
Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.
(15 Publications)
Species: Hu, Mu, Rt, Ca
Applications: WB, Flow, ICC/IF
5-HT1B Antibody
NB100-56350
Western Blot: 5-HT1B Antibody [NB100-56350] - Analysis of 5-HT1B in human brain lysate with anti-5-HT1BR pcAb (NB100-56350). A protein band of approximate molecular weight of 40-41kDa was detected.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB
5-HT2C Antibody
(496214)
MAB5764
A172 human glioblastoma cell line was stained with Mouse Anti-Human 5HT2C Monoclonal Antibody (Catalog # MAB5764, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
5HT2C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human 5HT2C Monoclonal Antibody (Catalog # MAB5764) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: Flow, IHC, CyTOF-ready
5-HT2B Antibody
NBP1-90322
Immunocytochemistry/Immunofluorescence: 5-HT2B Antibody [NBP1-90322] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining in human endometrium and skeletal muscle tissues. Corresponding HTR2B RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human endometrium shows moderate membranous positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Carbonic Anhydrase
I/CA1 Antibody
NBP1-88191
Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Comparative proteome analysis of 3xTg-AD and control samples at different stages of AD. Immunoblot analysis of most regulated hits. Soluble fractions of brain proteins were analyzed from four 2-month-old control and 3xTg-AD mice animals, respectively. Hebp1 and Glo1 levels were consistently elevated in the transgenic animals as compared to wild type controls. Ca1 levels were reduced in transgenic animals. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/47498), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.
Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human cell line HELA.
(3 Publications)
Species: Hu, Rt
Applications: WB, IHC, IHC-P
Substance P
Antibody
NB300-187
Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Staining of trigeminal ganglia from P3-P5 rat pups. BMC Neuroscience 2010, 11:32doi:10.1186/1471-2202-11-32
Immunohistochemistry: Substance P Antibody [NB300-187] - Spinal Cord Dorsal Horn (Human).
Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Detection of Substance P in rat spinal cord dorsal horn (red fluorescence). DAPI (blue) was used as counter stain.
(7 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC
5-HT1D Antibody
NLS590
Immunohistochemistry-Paraffin: 5-HT1D Antibody [NLS590] - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry-Paraffin: 5-HT1D Antibody [NLS590] - Analysis of anti-5HT1D Receptor antibody with human spinal cord, dorsal root ganglion.
Immunohistochemistry-Paraffin: 5-HT1D Antibody [NLS590] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Hu, Pm
Applications: IHC, IHC-P
G protein alpha
inhibitor 1 Antibody ...
H00002770-M01
Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Analysis of GNAI1 expression in transfected 293T cell line by GNAI1 monoclonal antibody (M01), clone 2B8-2A5.Lane 1: GNAI1 transfected lysate(40.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Analysis of monoclonal antibody to GNAI1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - GNAI1 monoclonal antibody (M01), clone 2B8-2A5. Analysis of GNAI1 expression in human liver.
(1 Publication)
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
POMC Antibody
NB100-1533
Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(10 Publications)
Species: Hu, Mu, Rt, Po, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA

SLC6A4/5-HTTLPR/Seroto ...
NB100-1892
Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Human Colon lysate, 35 ug protein in RIPA buffer. Antibody at 2 ug/mL. Detection by chemiluminescence.
Immunocytochemistry/Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, PEP-ELISA
DIO2 Antibody
NBP1-00178
Immunocytochemistry/Immunofluorescence: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Frozen: DIO2 Antibody [NBP1-00178] - IHC analysis of DIO2 in cryo-sectioned Mouse Hippocampus using NBP1-00178 at 2ug/ml. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.
Flow Cytometry: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody
(6 Publications)
Species: Hu, Mu, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA
ERMAP Antibody
AF4928
K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human ERMAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4928, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Species: Hu
Applications: WB, Flow, CyTOF-ready

Publications for 5-HT4 Antibody (NBP1-78403) (0)

There are no publications for 5-HT4 Antibody (NBP1-78403).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 5-HT4 Antibody (NBP1-78403) (0)

There are no reviews for 5-HT4 Antibody (NBP1-78403). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for 5-HT4 Antibody (NBP1-78403): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 5-HT4 Antibody (NBP1-78403) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-78403AF350
Alexa Fluor 405 NBP1-78403AF405
Alexa Fluor 488 NBP1-78403AF488
Alexa Fluor 532 NBP1-78403AF532
Alexa Fluor 594 NBP1-78403AF594
Alexa Fluor 647 NBP1-78403AF647
Alexa Fluor 700 NBP1-78403AF700
Alexa Fluor 750 NBP1-78403AF750
Biotin NBP1-78403B
DyLight 350 NBP1-78403UV
DyLight 405 NBP1-78403V
DyLight 488 NBP1-78403G
DyLight 550 NBP1-78403R
DyLight 594 NBP1-78403DL594
DyLight 650 NBP1-78403C
DyLight 680 NBP1-78403FR
DyLight 755 NBP1-78403IR
Janelia Fluor 549 NBP1-78403JF549
Janelia Fluor 646 NBP1-78403JF646
Learn about Custom Antibody Labeling

Additional 5-HT4 Products

Bioinformatics Tool for 5-HT4 Antibody (NBP1-78403)

Discover related pathways, diseases and genes to 5-HT4 Antibody (NBP1-78403). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 5-HT4 Antibody (NBP1-78403)

Discover more about diseases related to 5-HT4 Antibody (NBP1-78403).
 

Pathways for 5-HT4 Antibody (NBP1-78403)

View related products by pathway.

PTMs for 5-HT4 Antibody (NBP1-78403)

Learn more about PTMs related to 5-HT4 Antibody (NBP1-78403).
 

Research Areas for 5-HT4 Antibody (NBP1-78403)

Find related products by research area.

Blogs on 5-HT4

There are no specific blogs for 5-HT4, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  NBP1-78403 Specific Protocol
  Western Blot Protocol
  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our 5-HT4 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol HTR4
Entrez
Uniprot