5-HT4 Antibody

Images

 
Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunocytochemistry/ Immunofluorescence: 5-HT4 Antibody [NBP1-78403] - Staining of 5HT4 receptor in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.09 mg/ml

5-HT4 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human 5HT4 Receptor protein (between residues 350-388) [UniProt Q13639, isoform 1]
Localization
Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HTR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This 5HT4 Receptor antibody is useful for Immunocytochemistry/Immunofluorescence where cytoplasmic staining is observed in HeLa cells.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.09 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 5-HT4 Antibody

  • 5HT4
  • 5-HT4
  • 5-HT-4
  • 5-HT4R
  • 5-hydroxytryptamine (serotonin) receptor 4,5-hydroxytryptamine receptor 4,5-HT45-hydroxytryptamine4 receptor
  • cardiac 5-HT4 receptor
  • HTR4
  • Serotonin receptor 4

Background

The 5HT4 receptor (5-HT4R) is a member of the serotonin receptors. In response to serotonin (5HT) these G protein coupled receptors cause an increase in cAMP production. It is a glycosylated, transmembrane protein found in the central and peripheral nervous system. Multiple transcripts with variant C-terminal sequnces have been described. The significance of SNP variants of 5HT4 receptors in mental diseases is a controversial topic (PMID:19328558, 19892407), while other SNP variants have been associated with asthma.( PMID:21382128). Additional research show that the early presence of these receptors in the dorsomedial nuclei of prenatal hypothalamus may indicate a crucial role in fetal development. (PMID:20538346)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for 5-HT4 Antibody (NBP1-78403): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 5-HT4 Antibody (NBP1-78403) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-78403AF350
Alexa Fluor 405 NBP1-78403AF405
Alexa Fluor 488 NBP1-78403AF488
Alexa Fluor 532 NBP1-78403AF532
Alexa Fluor 594 NBP1-78403AF594
Alexa Fluor 647 NBP1-78403AF647
Alexa Fluor 700 NBP1-78403AF700
Alexa Fluor 750 NBP1-78403AF750
Biotin NBP1-78403B
DyLight 350 NBP1-78403UV
DyLight 405 NBP1-78403V
DyLight 488 NBP1-78403G
DyLight 550 NBP1-78403R
DyLight 594 NBP1-78403DL594
DyLight 650 NBP1-78403C
DyLight 680 NBP1-78403FR
DyLight 755 NBP1-78403IR
Janelia Fluor 549 NBP1-78403JF549
Janelia Fluor 646 NBP1-78403JF646

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Bioinformatics

Gene Symbol HTR4
Entrez
Uniprot