5-HT7 Antibody

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Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

5-HT7 Antibody Summary

Immunogen
This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 13-28 of the rat 5-HT7R (AAA42134.1).
Specificity
This sequence is identical for 5-HT7R splice variants in the rat (5-HT7Ra/b/c), human (5-HT7Ra/b/d) and human 5-HT7, the mouse 5-HT7R. It is 93% conserved with dog 5-HT7Ra/b, and 81% conserved with pig 5-HT7R. The 5HT7 antibody recognizes all described 5HT7 receptor splice variants.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HTR7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Western Blot 1-2 ug/ml
Application Notes
In human brain, a 50 kDa band is observed. Both 45 kDa and 50 kDa bands have been observed in various human glioblastoma cell lines and in the human microglial MC-3 cell lines. Bands in the 45-50 kDa range correspond to the predicted molecular weight for the 5HT7 receptor. The 5HT7 receptor also has putative sites for N-linked glycosylation and phosphorylation which may lead to variations in observed molecular weights. Use in Immunocytochemistry/Immunoflourescence was reported in the literature (PMID: 17940054). Use in FLOW reported in scientific literature (PMID: 30602786).The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-56352 in the following applications:

  • 1 publication
  • 3 publications
  • WB
    14 publications

Reactivity Notes

Predicted to react with Porcine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for 5-HT7 Antibody

  • 5HT7
  • 5-HT7
  • 5-HT-7
  • 5-HT75-hydroxytryptamine receptor 7
  • 5-HT-X
  • 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
  • HTR7
  • serotonin 5-HT-7 receptor
  • Serotonin receptor 7

Background

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5-HT7 Antibody (NB100-56352)(17)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Mouse/Human.

We have publications tested in 3 applications: Flow, ICC/IF, WB.


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Flow
(1)
ICC/IF
(3)
WB
(14)
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Human
(6)
Mouse
(5)
Rat
(4)
Mouse/Human
(1)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-56352 Applications Species
Cho SY, Ki HG, Kim JM et al. Expression of the spinal 5-HT7 receptor and p-ERK pathway in the carrageenan inflammatory pain of rats Korean J Anesthesiol 2015 Apr 01 [PMID: 25844136] (WB, Rat)

Details:
5-HT7 antibody used for WB on 30 ug lysate of spinal cord samples /the ipsilateral dorsal spinal cord at L4-L6 obtained from rats that were subjected or not to induce the inflammatory pain via subcutaneous injection of carrageenan (degraded gamma-Carrageenan) at the center of their hind paw (Fig 1).
WB Rat
Strekalova E, Profirovic J. Serotonin 5-HT7 Receptor Regulates Endothelial Cell Migration via Protein Kinase A Austin J Pharmacol Ther et al. 2014 Sep 25 (WB, Human)

Details:
Rabbit polyclonal anti-5-HT7R antibody used for WB on lysate of HUVEC cells (Figure 1B)
WB Human
Okada M, Matsumoto R, Yamamoto Y, Fukuyama K Effects of Subchronic Administrations of Vortioxetine, Lurasidone, and Escitalopram on Thalamocortical Glutamatergic Transmission Associated with Serotonin 5-HT7 Receptor International journal of molecular sciences Jan 29 2021 [PMID: 33572981] (WB, Rat) WB Rat
Tempio A, Niso M, Laera L et al. Mitochondrial Membranes of Human SH-SY5Y Neuroblastoma Cells Express Serotonin 5-HT7 Receptor International journal of molecular sciences Dec 17 2020 [PMID: 33348850] (WB, Human) WB Human
Volpicelli F, Speranza L, Pulcrano S et al. The microRNA-29a Modulates Serotonin 5-HT7 Receptor Expression and Its Effects on Hippocampal Neuronal Morphology Mol. Neurobiol. Jul 10 2019 [PMID: 31292861] (WB, Mouse) WB Mouse
Ito M, Komai K, Mise-Omata S et al. Brain regulatory T cells suppress astrogliosis and potentiate neurological recovery. Nature Jan 1 2019 [PMID: 30602786] (Flow, Mouse) Flow Mouse
Wixey JA, Reinebrant HE, Chand KK, Buller KM. Disruption to the 5-HT7 Receptor Following Hypoxia-Ischemia in the Immature Rodent Brain Neurochem. Res. 2018 Jan 22 [PMID: 29357019] (WB, Rat) WB Rat
Gautam J, Banskota S, Regmi SC et al. Tryptophan hydroxylase 1 and 5-HT7 receptor preferentially expressed in triple-negative breast cancer promote cancer progression through autocrine serotonin signaling. Mol. Cancer. Nov 21 2016 [PMID: 27871326] (WB, Human) WB Human
Holst K, Guseva D, Schindler S et al. Serotonin receptor 5-HT7 regulates morphology and migratory properties of dendritic cells J. Cell. Sci. 2015 Jun 19 [PMID: 26092936] (ICC/IF, Mouse) ICC/IF Mouse
Speranza L, Giuliano T, Volpicelli F et al. Activation of 5-HT7 receptor stimulates neurite elongation through mTOR, Cdc42 and actin filaments dynamics Front Behav Neurosci 2015 Mar 27 [PMID: 25814944] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Show All 17 Publications.

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Bioinformatics

Gene Symbol HTR7
Entrez
Uniprot