5-HT7 Antibody


Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.

Product Details

Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB
1.0 mg/ml

Order Details

5-HT7 Antibody Summary

This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 13-28 of the rat 5-HT7R (AAA42134.1).
This sequence is identical for 5-HT7R splice variants in the rat (5-HT7Ra/b/c), human (5-HT7Ra/b/d) and human 5-HT7, the mouse 5-HT7R. It is 93% conserved with dog 5-HT7Ra/b, and 81% conserved with pig 5-HT7R. The 5HT7 antibody recognizes all described 5HT7 receptor splice variants.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
In human brain, a 50 kDa band is observed. Both 45 kDa and 50 kDa bands have been observed in various human glioblastoma cell lines and in the human microglial MC-3 cell lines. Bands in the 45-50 kDa range correspond to the predicted molecular weight for the 5HT7 receptor. The 5HT7 receptor also has putative sites for N-linked glycosylation and phosphorylation which may lead to variations in observed molecular weights. Use in Immunocytochemistry/Immunoflourescence was reported in the literature (PMID: 17940054) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB100-56352 in the following applications:

  • 3 publications
  • WB
    11 publications

Reactivity Notes

Predicted to react with Porcine.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for 5-HT7 Antibody

  • 5HT7
  • 5-HT7
  • 5-HT-7
  • 5-HT75-hydroxytryptamine receptor 7
  • 5-HT-X
  • 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
  • HTR7
  • serotonin 5-HT-7 receptor
  • Serotonin receptor 7


Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Mu, Rt
Applications: WB, IHC
Species: Hu
Applications: WB, ICC/IF
Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: Flow, IHC, CyTOF-ready
Species: Hu, Mu
Applications: WB, ICC/IF
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western
Species: Hu, Mu, Po, Ch, RM
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Bv, Pm, Sh, Xp
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Supplier Logo

Publications for 5-HT7 Antibody (NB100-56352)(13)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Mouse/Human.

We have publications tested in 2 applications: ICC/IF, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-56352 Applications Species
Wixey JA, Reinebrant HE, Chand KK, Buller KM. Disruption to the 5-HT7 Receptor Following Hypoxia-Ischemia in the Immature Rodent Brain Neurochem. Res. 2018 Jan 22 [PMID: 29357019] (WB, Rat) WB Rat
Gautam J, Banskota S, Regmi SC et al. Tryptophan hydroxylase 1 and 5-HT7 receptor preferentially expressed in triple-negative breast cancer promote cancer progression through autocrine serotonin signaling. Mol. Cancer. Nov 21 2016 [PMID: 27871326] (WB, Human) WB Human
Holst K, Guseva D, Schindler S et al. Serotonin receptor 5-HT7 regulates morphology and migratory properties of dendritic cells J. Cell. Sci. 2015 Jun 19 [PMID: 26092936] (ICC/IF, Mouse) ICC/IF Mouse
Cho SY, Ki HG, Kim JM et al. Expression of the spinal 5-HT7 receptor and p-ERK pathway in the carrageenan inflammatory pain of rats Korean J Anesthesiol 2015 Apr 01 [PMID: 25844136] (WB, Rat)

5-HT7 antibody used for WB on 30 ug lysate of spinal cord samples /the ipsilateral dorsal spinal cord at L4-L6 obtained from rats that were subjected or not to induce the inflammatory pain via subcutaneous injection of carrageenan (degraded gamma-Carrageenan) at the center of their hind paw (Fig 1).
WB Rat
Garcia-Iglesias BB, Mendoza-Garrido ME, Gutierrez-Ospina G et al. Sensitization of restraint-induced corticosterone secretion after chronic restraint in rats: Involvement of 5-HT7 receptors Neuropharmacology 2013 Mar 28 [PMID: 23542440] (WB, Rat) WB Rat
Amaya-Castellanos E, Pineda-Farias JB, Castaneda-Corral G et al. Blockade of 5-HT(7) receptors reduces tactile allodynia in the rat. Pharmacol Biochem Behav. 2011 Jun 15. [PMID: 21693130]
Pai VP, Horseman ND. Biphasic regulation of mammary epithelial resistance by serotonin through activation of multiple pathways. J Biol Chem. 2008 Nov 7 [PMID: 18782769] (WB, Human)

WB (human mammary MCF10A cells), Fig. 1C.
WB Human
Stull MA, Pai V, Vomachka AJ et al. Mammary gland homeostasis employs serotonergic regulation of epithelial tight junctions. Proc Natl Acad Sci U S A. 2007 Oct 16 [PMID: 17940054] (WB, ICC/IF, Mouse/Human)

Product cited: Serotonin 5-HT7 Receptor (IMG-368), Mouse Brain Tissue Lysate (40101), & Human Brain Tissue Lysate (40141). 1. WB: Human MCF10A mammary epithelial cells, mouse primary mammary epithelial cells (mPMEC), human brain (40141), & mouse brain (40
WB, ICC/IF Mouse/Human
Leon-Ponte M, Ahern GP, O'Connell PJ. Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor. Blood. 2007 Apr 15 [PMID: 17158224] (WB, Mouse)

WB (mouse brain and hypothalamus tissue, mouse T cells purified from spleen, Fig 2): IMG-366 (5-HT1BR) and IMG-368 (5-HT7R). Note: Levels of 5-HT1BR and 5-HT7R increased following T-cell activation by Con A.
WB Mouse
Mahe C, Loetscher E, Dev KK et al. Serotonin 5-HT7 receptors coupled to induction of interleukin-6 in human microglial MC-3 cells. Neuropharmacology. 2005 Jul [PMID: 15992579] (WB, Human)

WB: Fig 3a (untransfected CHO cells/CHO cells transfected with the human 5-HT7aR), Fig 3b (untransfected CHO cells, human microglial MC-3 cells). Notes: Two bands of ~45 and 50 kDa were seen in the 5-HT7aR tably transfected cells and the MC-3 cells. These
WB Human
Show All 13 Publications.

Reviews for 5-HT7 Antibody (NB100-56352) (0)

There are no reviews for 5-HT7 Antibody (NB100-56352). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 5-HT7 Antibody (NB100-56352) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional 5-HT7 Products

Bioinformatics Tool for 5-HT7 Antibody (NB100-56352)

Discover related pathways, diseases and genes to 5-HT7 Antibody (NB100-56352). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 5-HT7 Antibody (NB100-56352)

Discover more about diseases related to 5-HT7 Antibody (NB100-56352).

Pathways for 5-HT7 Antibody (NB100-56352)

View related products by pathway.

PTMs for 5-HT7 Antibody (NB100-56352)

Learn more about PTMs related to 5-HT7 Antibody (NB100-56352).

Research Areas for 5-HT7 Antibody (NB100-56352)

Find related products by research area.

Blogs on 5-HT7

There are no specific blogs for 5-HT7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our 5-HT7 Antibody and receive a gift card or discount.


Gene Symbol HTR7