5-HT7 Antibody

Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.

Simple Western: 5-HT7 Antibody [NB100-56352] - Panel (B) indicates the pseudo-gel images using capillary immunoblotting. * p < 0.05, ** p < 0.01 vs. the control by Student's t-test. Image collected and cropped by CiteAb from the following publication (h//pubmed.ncbi.nlm.nih.gov/33572981/) licensed under a CC-BY license.

Western Blot: 5-HT7 Antibody [NB100-56352] - Effects of subchronic administration (7 and 14 days) of therapeutic relevant concentration of Brex (Brex: 300 nM) and interaction between Brex and 5-HT1A receptor (5-HT1AR) antagonist WAY100635 (WAY: 10 uM) on protein expression of 5-HT7 receptor in the plasma membrane fraction of cortical primary cultured astrocytes. In left side histograms, ordinate: mean +/- SD (n = 6) of the relative protein level of 5-HT7R per GAPDH. * p < 0.05, ** p < 0.01: relative to control (Brex-free) by one-way analysis of variance (ANOVA) with Tukey’s post-hoc test, and @ p < 0.05: relative to Brex for 14 days by Student’s T-test. Right side panels indicate their pseudo-gel images using capillary immunoblotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35743014/) licensed under a CC-BY license.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Simple Western, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

5-HT7 Antibody Summary

• Immunogen: This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 13-28 of the rat 5-HT7R (AAA42134.1).
• Specificity: The 5HT7 antibody recognizes all described 5HT7 receptor splice variants.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTR7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature (PMID 30602786)
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000. Use reported in scientific literature (PMID 17940054)
  • Simple Western
  • Western Blot 1-2 ug/ml
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This sequence is identical for 5-HT7R splice variants in the rat (5-HT7Ra/b/c), human (5-HT7Ra/b/d) and human 5-HT7, the mouse 5-HT7R. It is 93% conserved with canine 5-HT7Ra/b, and 81% conserved with porcine 5-HT7R.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for 5-HT7 Antibody

• 5HT7
• 5-HT7
• 5-HT-7
• 5-HT75-hydroxytryptamine receptor 7
• 5-HT-X
• 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
• HTR7
• serotonin 5-HT-7 receptor
• Serotonin receptor 7

Background

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT7 Antibody (NB100-56352)(22)

Publications using NB100-56352

• Yang JS, Gao FF, Yang XX et al. The 5-HT7 receptors in the VLO contribute to the development of morphine-induced behavioral sensitization in rats Neurochemistry international 2023-06-18 [PMID: 37339717] (WB, Rat)
  
  Details:
  1:100 dilution
• Okada M, Fukuyama K, Motomura E Dose-Dependent Biphasic Action of Quetiapine on AMPK Signalling via 5-HT7 Receptor: Exploring Pathophysiology of Clinical and Adverse Effects of Quetiapine International journal of molecular sciences 2022-08-14 [PMID: 36012369] (Simple Western, Rat)
  
  Details:
  Dilution used 1:300
• Fukuyama K, Motomura E, Okada M Brexpiprazole Reduces 5-HT7 Receptor Function on Astroglial Transmission Systems International journal of molecular sciences 2022-06-12 [PMID: 35743014] (Simple Western, WB, Rat)
• Fukuyama K, Motomura E, Shiroyama T, Okada M Impact of 5-HT7 receptor inverse agonism of lurasidone on monoaminergic tripartite synaptic transmission and pathophysiology of lower risk of weight gain Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022-02-24 [PMID: 35219120] (WB, Rat)
• Chaudhary P, Guragain D, Chang J, Kim J TPH1 and 5-HT7 Receptor Overexpression Leading to Gemcitabine-Resistance Requires Non-Canonical Permissive Action of EZH2 in Pancreatic Ductal Adenocarcinoma Cancers 2021-10-22 [PMID: 34771469] (WB, Human)
• Okada M, Matsumoto R, Yamamoto Y, Fukuyama K Effects of Subchronic Administrations of Vortioxetine, Lurasidone, and Escitalopram on Thalamocortical Glutamatergic Transmission Associated with Serotonin 5-HT7 Receptor International journal of molecular sciences 2021-01-29 [PMID: 33572981] (WB, Rat)
• Tempio A, Niso M, Laera L et al. Mitochondrial Membranes of Human SH-SY5Y Neuroblastoma Cells Express Serotonin 5-HT7 Receptor International journal of molecular sciences 2020-12-17 [PMID: 33348850] (WB, Human)
• Volpicelli F, Speranza L, Pulcrano S et al. The microRNA-29a Modulates Serotonin 5-HT7 Receptor Expression and Its Effects on Hippocampal Neuronal Morphology Mol. Neurobiol. 2019-07-10 [PMID: 31292861] (WB, Mouse)
• Ito M, Komai K, Mise-Omata S Brain regulatory T cells suppress astrogliosis and potentiate neurological recovery Nature 2019-01-02 [PMID: 30602786] (FLOW, Mouse)
• Wixey JA, Reinebrant HE, Chand KK, Buller KM. Disruption to the 5-HT7 Receptor Following Hypoxia-Ischemia in the Immature Rodent Brain Neurochem. Res. 2018-01-22 [PMID: 29357019] (WB, Rat)
• Gautam J, Banskota S, Regmi SC et al. Tryptophan hydroxylase 1 and 5-HT7 receptor preferentially expressed in triple-negative breast cancer promote cancer progression through autocrine serotonin signaling. Mol. Cancer. 2016-11-21 [PMID: 27871326] (WB, Human)
• Holst K, Guseva D, Schindler S et al. Serotonin receptor 5-HT7 regulates morphology and migratory properties of dendritic cells J. Cell. Sci. 2015-06-19 [PMID: 26092936] (ICC/IF, Mouse)
• Cho SY, Ki HG, Kim JM et al. Expression of the spinal 5-HT7 receptor and p-ERK pathway in the carrageenan inflammatory pain of rats Korean J Anesthesiol 2015-04-01 [PMID: 25844136] (WB, Rat)
  
  Details:
  5-HT7 antibody used for WB on 30 ug lysate of spinal cord samples /the ipsilateral dorsal spinal cord at L4-L6 obtained from rats that were subjected or not to induce the inflammatory pain via subcutaneous injection of carrageenan (degraded gamma-Carrageenan) at the center of their hind paw (Fig 1).
• Strekalova E, Profirovic J. Serotonin 5-HT7 Receptor Regulates Endothelial Cell Migration via Protein Kinase A Austin J Pharmacol Ther et al. 2014-09-25 (WB, Human)
  
  Details:
  Rabbit polyclonal anti-5-HT7R antibody used for WB on lysate of HUVEC cells (Figure 1B)
• Speranza L, Giuliano T, Volpicelli F et al. Activation of 5-HT7 receptor stimulates neurite elongation through mTOR, Cdc42 and actin filaments dynamics Front Behav Neurosci 2015-03-27 [PMID: 25814944] (ICC/IF, WB, Mouse)
• Garcia-Iglesias BB, Mendoza-Garrido ME, Gutierrez-Ospina G et al. Sensitization of restraint-induced corticosterone secretion after chronic restraint in rats: Involvement of 5-HT7 receptors Neuropharmacology 2013-03-28 [PMID: 23542440] (WB, Rat)
• Amaya-Castellanos E, Pineda-Farias JB, Castaneda-Corral G et al. Blockade of 5-HT(7) receptors reduces tactile allodynia in the rat. Pharmacol Biochem Behav. 2011-06-15 [PMID: 21693130]
• Pai VP, Horseman ND. Biphasic regulation of mammary epithelial resistance by serotonin through activation of multiple pathways. J Biol Chem. 2008-11-07 [PMID: 18782769] (WB, Human)
  
  Details:
  WB (human mammary MCF10A cells), Fig. 1C.
• Stull MA, Pai V, Vomachka AJ et al. Mammary gland homeostasis employs serotonergic regulation of epithelial tight junctions. Proc Natl Acad Sci U S A. 2007-10-16 [PMID: 17940054] (ICC/IF, WB, Mouse Serum)
  
  Details:
  Serotonin 5-HT7 Receptor (IMG-368), Mouse Brain Tissue Lysate (40101), & Human Brain Tissue Lysate (40141). 1. WB: Human MCF10A mammary epithelial cells, mouse primary mammary epithelial cells (mPMEC), human brain (40141), & mouse brain (40101) were probed with 5-HT7 (IMG-368) antibody, Fig 1Bii.
• Ponte M, Ahern G, O'Connell P Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor Blood. [PMID: 17158224] (WB, Mouse)
• Mahe C, Loetscher E, Dev KK et al. Serotonin 5-HT7 receptors coupled to induction of interleukin-6 in human microglial MC-3 cells. Neuropharmacology. 2005-07-01 [PMID: 15992579] (WB, Human)
  
  Details:
  WB: Fig 3a (untransfected CHO cells/CHO cells transfected with the human 5-HT7aR), Fig 3b (untransfected CHO cells, human microglial MC-3 cells). Notes: Two bands of ~45 and 50 kDa were seen in the 5-HT7aR tably transfected cells and the MC-3 cells. These
• Mahe C, Bernhard M, Bobirnac I et al. Functional expression of the serotonin 5-HT7 receptor in human glioblastoma cell lines. Br J Pharmacol. 2004-10-01 [PMID: 15339860] (WB, Human)
  
  Details:
  WB (Fig 3), 5-HT7aR stably transfected/untransfected CHO cells, human glioblastoma cell lines (T98G, H4 cells; U-373 MG; U-138 MG, DBTRG-05MG, U-87 MG, Hs 683, CCF-STTG1). Notes: Two bands of ~45 and 50 kDa were seen in the 5-HT7a R stably transfected cel

Bioinformatics

• Gene Symbol: HTR7
• Entrez:
  • 3363(Human)
  • 15566(Mouse)
  • 65032(Rat)
• Uniprot:
  • P32305(Rat)