5-HT7 Antibody


Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.

Product Details

Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF
1.0 mg/ml

Order Details

5-HT7 Antibody Summary

This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 13-28 of the rat 5-HT7R (AAA42134.1).
This sequence is identical for 5-HT7R splice variants in the rat (5-HT7Ra/b/c), human (5-HT7Ra/b/d) and human 5-HT7, the mouse 5-HT7R. It is 93% conserved with dog 5-HT7Ra/b, and 81% conserved with pig 5-HT7R. The 5HT7 antibody recognizes all described 5HT7 receptor splice variants.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified


  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
In human brain, a 50 kDa band is observed. Both 45 kDa and 50 kDa bands have been observed in various human glioblastoma cell lines and in the human microglial MC-3 cell lines. Bands in the 45-50 kDa range correspond to the predicted molecular weight for the 5HT7 receptor. The 5HT7 receptor also has putative sites for N-linked glycosylation and phosphorylation which may lead to variations in observed molecular weights. Use in Immunocytochemistry/Immunoflourescence was reported in the literature (PMID: 17940054) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
54 kDa
Read Publications using
NB100-56352 in the following applications:

  • 3 publications
  • WB
    9 publications

Reactivity Notes

Predicted to react with Porcine.

Alternate Names for 5-HT7 Antibody

  • 5HT7
  • 5-HT-7
  • 5-HT75-hydroxytryptamine receptor 7
  • 5-HT-X
  • 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
  • HTR7
  • serotonin 5-HT-7 receptor
  • Serotonin receptor 7


Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Mu, Rt
Applications: WB, IHC
Species: Hu, Bt, Bv, Ca, Eq, GP, Ha, Rb
Applications: IHC-P
Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: Flow, IHC
Species: Hu, Mu
Applications: WB, ICC/IF
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western
Species: Hu, Ch, RM
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye, Am(-), Av(-), Pm(-)
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
Species: Hu, Rt
Applications: ELISA, IP, S-ELISA
Species: Hu, Ye
Applications: WB
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P, PEP-ELISA
Supplier Logo

Publications for 5-HT7 Antibody (NB100-56352)(11)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Mouse/Human.

We have publications tested in 2 applications: ICC/IF, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-56352 Applications Species
Holst K, Guseva D, Schindler S et al. Serotonin receptor 5-HT7 regulates morphology and migratory properties of dendritic cells J. Cell. Sci. 2015 Jun 19 [PMID:26092936] (ICC/IF, Mouse) ICC/IF Mouse
Cho SY, Ki HG, Kim JM et al. Expression of the spinal 5-HT7 receptor and p-ERK pathway in the carrageenan inflammatory pain of rats Korean J Anesthesiol 2015 Apr 01 [PMID:25844136] (WB, Rat) WB Rat
Speranza L, Giuliano T, Volpicelli F et al. Activation of 5-HT7 receptor stimulates neurite elongation through mTOR, Cdc42 and actin filaments dynamics Front Behav Neurosci 2015 Mar 27 [PMID:25814944] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Garcia-Iglesias BB, Mendoza-Garrido ME, Gutierrez-Ospina G et al. Sensitization of restraint-induced corticosterone secretion after chronic restraint in rats: Involvement of 5-HT7 receptors Neuropharmacology 2013 Mar 28 [PMID:23542440] (WB, Rat) WB Rat
Amaya-Castellanos E, Pineda-Farias JB, Castaneda-Corral G et al. Blockade of 5-HT(7) receptors reduces tactile allodynia in the rat. Pharmacol Biochem Behav. 2011 Jun 15. [PMID:21693130]
Pai VP, Horseman ND. Biphasic regulation of mammary epithelial resistance by serotonin through activation of multiple pathways. J Biol Chem. 2008 Nov 7 [PMID:18782769] (WB, Human) WB Human
Stull MA, Pai V, Vomachka AJ et al. Mammary gland homeostasis employs serotonergic regulation of epithelial tight junctions. Proc Natl Acad Sci U S A. 2007 Oct 16 [PMID:17940054] (WB, ICC/IF, Mouse/Human) WB, ICC/IF Mouse/Human
Leon-Ponte M, Ahern GP, O'Connell PJ. Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor. Blood. 2007 Apr 15 [PMID:17158224] (WB, Mouse) WB Mouse
Mahe C, Loetscher E, Dev KK et al. Serotonin 5-HT7 receptors coupled to induction of interleukin-6 in human microglial MC-3 cells. Neuropharmacology. 2005 Jul [PMID:15992579] (WB, Human) WB Human
Mahe C, Bernhard M, Bobirnac I et al. Functional expression of the serotonin 5-HT7 receptor in human glioblastoma cell lines. Br J Pharmacol. 2004 Oct [PMID:15339860] (WB, Human) WB Human
Show All 11 Publications.

Reviews for 5-HT7 Antibody (NB100-56352) (0)

There are no reviews for 5-HT7 Antibody (NB100-56352). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 5-HT7 Antibody (NB100-56352) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional 5-HT7 Antibody Products

Related Products by Gene

Bioinformatics Tool for 5-HT7 Antibody (NB100-56352)

Discover related pathways, diseases and genes to 5-HT7 Antibody (NB100-56352). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 5-HT7 Antibody (NB100-56352)

Discover more about diseases related to 5-HT7 Antibody (NB100-56352).

Pathways for 5-HT7 Antibody (NB100-56352)

View related products by pathway.

PTMs for 5-HT7 Antibody (NB100-56352)

Learn more about PTMs related to 5-HT7 Antibody (NB100-56352).

Research Areas for 5-HT7 Antibody (NB100-56352)

Find related products by research area.

Blogs on 5-HT7

There are no specific blogs for 5-HT7, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol HTR7

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-56352 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought