5-HT7 Antibody

Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

5-HT7 Antibody Summary

• Immunogen: This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 13-28 of the rat 5-HT7R (AAA42134.1).
• Specificity: This sequence is identical for 5-HT7R splice variants in the rat (5-HT7Ra/b/c), human (5-HT7Ra/b/d) and human 5-HT7, the mouse 5-HT7R. It is 93% conserved with dog 5-HT7Ra/b, and 81% conserved with pig 5-HT7R. The 5HT7 antibody recognizes all described 5HT7 receptor splice variants.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTR7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Western Blot 1-2 ug/ml
• Application Notes: In human brain, a 50 kDa band is observed. Both 45 kDa and 50 kDa bands have been observed in various human glioblastoma cell lines and in the human microglial MC-3 cell lines. Bands in the 45-50 kDa range correspond to the predicted molecular weight for the 5HT7 receptor. The 5HT7 receptor also has putative sites for N-linked glycosylation and phosphorylation which may lead to variations in observed molecular weights. Use in Immunocytochemistry/Immunoflourescence was reported in the literature (PMID: 17940054). Use in FLOW reported in scientific literature (PMID: 30602786).The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Porcine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for 5-HT7 Antibody

• 5HT7
• 5-HT7
• 5-HT-7
• 5-HT75-hydroxytryptamine receptor 7
• 5-HT-X
• 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
• HTR7
• serotonin 5-HT-7 receptor
• Serotonin receptor 7

Background

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT7 Antibody (NB100-56352)(17)

Publications using NB100-56352

• Cho SY, Ki HG, Kim JM et al. Expression of the spinal 5-HT7 receptor and p-ERK pathway in the carrageenan inflammatory pain of rats Korean J Anesthesiol 2015 Apr 01 [PMID: 25844136] (WB, Rat)
  
  Details:
  5-HT7 antibody used for WB on 30 ug lysate of spinal cord samples /the ipsilateral dorsal spinal cord at L4-L6 obtained from rats that were subjected or not to induce the inflammatory pain via subcutaneous injection of carrageenan (degraded gamma-Carrageenan) at the center of their hind paw (Fig 1).
• Strekalova E, Profirovic J. Serotonin 5-HT7 Receptor Regulates Endothelial Cell Migration via Protein Kinase A Austin J Pharmacol Ther et al. 2014 Sep 25 (WB, Human)
  
  Details:
  Rabbit polyclonal anti-5-HT7R antibody used for WB on lysate of HUVEC cells (Figure 1B)
• Okada M, Matsumoto R, Yamamoto Y, Fukuyama K Effects of Subchronic Administrations of Vortioxetine, Lurasidone, and Escitalopram on Thalamocortical Glutamatergic Transmission Associated with Serotonin 5-HT7 Receptor International journal of molecular sciences Jan 29 2021 [PMID: 33572981] (WB, Rat)
• Tempio A, Niso M, Laera L et al. Mitochondrial Membranes of Human SH-SY5Y Neuroblastoma Cells Express Serotonin 5-HT7 Receptor International journal of molecular sciences Dec 17 2020 [PMID: 33348850] (WB, Human)
• Volpicelli F, Speranza L, Pulcrano S et al. The microRNA-29a Modulates Serotonin 5-HT7 Receptor Expression and Its Effects on Hippocampal Neuronal Morphology Mol. Neurobiol. Jul 10 2019 [PMID: 31292861] (WB, Mouse)
• Ito M, Komai K, Mise-Omata S et al. Brain regulatory T cells suppress astrogliosis and potentiate neurological recovery. Nature Jan 1 2019 [PMID: 30602786] (Flow, Mouse)
• Wixey JA, Reinebrant HE, Chand KK, Buller KM. Disruption to the 5-HT7 Receptor Following Hypoxia-Ischemia in the Immature Rodent Brain Neurochem. Res. 2018 Jan 22 [PMID: 29357019] (WB, Rat)
• Gautam J, Banskota S, Regmi SC et al. Tryptophan hydroxylase 1 and 5-HT7 receptor preferentially expressed in triple-negative breast cancer promote cancer progression through autocrine serotonin signaling. Mol. Cancer. Nov 21 2016 [PMID: 27871326] (WB, Human)
• Holst K, Guseva D, Schindler S et al. Serotonin receptor 5-HT7 regulates morphology and migratory properties of dendritic cells J. Cell. Sci. 2015 Jun 19 [PMID: 26092936] (ICC/IF, Mouse)
• Speranza L, Giuliano T, Volpicelli F et al. Activation of 5-HT7 receptor stimulates neurite elongation through mTOR, Cdc42 and actin filaments dynamics Front Behav Neurosci 2015 Mar 27 [PMID: 25814944] (WB, ICC/IF, Mouse)
• Garcia-Iglesias BB, Mendoza-Garrido ME, Gutierrez-Ospina G et al. Sensitization of restraint-induced corticosterone secretion after chronic restraint in rats: Involvement of 5-HT7 receptors Neuropharmacology 2013 Mar 28 [PMID: 23542440] (WB, Rat)
• Amaya-Castellanos E, Pineda-Farias JB, Castaneda-Corral G et al. Blockade of 5-HT(7) receptors reduces tactile allodynia in the rat. Pharmacol Biochem Behav. 2011 Jun 15. [PMID: 21693130]
• Pai VP, Horseman ND. Biphasic regulation of mammary epithelial resistance by serotonin through activation of multiple pathways. J Biol Chem. 2008 Nov 7 [PMID: 18782769] (WB, Human)
  
  Details:
  WB (human mammary MCF10A cells), Fig. 1C.
• Stull MA, Pai V, Vomachka AJ et al. Mammary gland homeostasis employs serotonergic regulation of epithelial tight junctions. Proc Natl Acad Sci U S A. 2007 Oct 16 [PMID: 17940054] (WB, ICC/IF, Mouse/Human)
  
  Details:
  Product cited: Serotonin 5-HT7 Receptor (IMG-368), Mouse Brain Tissue Lysate (40101), & Human Brain Tissue Lysate (40141). 1. WB: Human MCF10A mammary epithelial cells, mouse primary mammary epithelial cells (mPMEC), human brain (40141), & mouse brain (40
• Leon-Ponte M, Ahern GP, O'Connell PJ. Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor. Blood. 2007 Apr 15 [PMID: 17158224] (WB, Mouse)
  
  Details:
  WB (mouse brain and hypothalamus tissue, mouse T cells purified from spleen, Fig 2): IMG-366 (5-HT1BR) and IMG-368 (5-HT7R). Note: Levels of 5-HT1BR and 5-HT7R increased following T-cell activation by Con A.
• Mahe C, Loetscher E, Dev KK et al. Serotonin 5-HT7 receptors coupled to induction of interleukin-6 in human microglial MC-3 cells. Neuropharmacology. 2005 Jul [PMID: 15992579] (WB, Human)
  
  Details:
  WB: Fig 3a (untransfected CHO cells/CHO cells transfected with the human 5-HT7aR), Fig 3b (untransfected CHO cells, human microglial MC-3 cells). Notes: Two bands of ~45 and 50 kDa were seen in the 5-HT7aR tably transfected cells and the MC-3 cells. These
• Mahe C, Bernhard M, Bobirnac I et al. Functional expression of the serotonin 5-HT7 receptor in human glioblastoma cell lines. Br J Pharmacol. 2004 Oct [PMID: 15339860] (WB, Human)
  
  Details:
  WB (Fig 3), 5-HT7aR stably transfected/untransfected CHO cells, human glioblastoma cell lines (T98G, H4 cells; U-373 MG; U-138 MG, DBTRG-05MG, U-87 MG, Hs 683, CCF-STTG1). Notes: Two bands of ~45 and 50 kDa were seen in the 5-HT7a R stably transfected cel

Bioinformatics

• Gene Symbol: HTR7
• Entrez:
  • 3363(Human)
  • 15566(Mouse)
  • 65032(Rat)
• Uniprot:
  • P32305(Rat)