5-HT7 Antibody


Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.
Biological Strategies: Simple Western: 5-HT7 Antibody [NB100-56352] - Panel (B) indicates the pseudo-gel images using capillary immunoblotting. * p < 0.05, ** p < 0.01 vs. the control by Student's t-test. Image ...read more
Biological Strategies: Western Blot: 5-HT7 Antibody [NB100-56352] - Effects of subchronic administration (7 and 14 days) of therapeutic relevant concentration of Brex (Brex: 300 nM) and interaction between Brex ...read more

Product Details

Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF
1.0 mg/ml
Validated by:

Biological Strategies


Order Details

5-HT7 Antibody Summary

This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 13-28 of the rat 5-HT7R (AAA42134.1).
The 5HT7 antibody recognizes all described 5HT7 receptor splice variants.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry reported in scientific literature (PMID 30602786)
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000. Use reported in scientific literature (PMID 17940054)
  • Simple Western
  • Western Blot 1-2 ug/ml
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB100-56352 in the following applications:

Reactivity Notes

This sequence is identical for 5-HT7R splice variants in the rat (5-HT7Ra/b/c), human (5-HT7Ra/b/d) and human 5-HT7, the mouse 5-HT7R. It is 93% conserved with canine 5-HT7Ra/b, and 81% conserved with porcine 5-HT7R.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for 5-HT7 Antibody

  • 5HT7
  • 5-HT7
  • 5-HT-7
  • 5-HT75-hydroxytryptamine receptor 7
  • 5-HT-X
  • 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
  • HTR7
  • serotonin 5-HT-7 receptor
  • Serotonin receptor 7


Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5-HT7 Antibody (NB100-56352)(22)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Mouse Serum.

We have publications tested in 4 applications: FLOW, ICC/IF, Simple Western, WB.

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Simple Western
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Mouse Serum
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB100-56352 Applications Species
Yang JS, Gao FF, Yang XX et al. The 5-HT7 receptors in the VLO contribute to the development of morphine-induced behavioral sensitization in rats Neurochemistry international 2023-06-18 [PMID: 37339717] (WB, Rat)

1:100 dilution
WB Rat
Okada M, Fukuyama K, Motomura E Dose-Dependent Biphasic Action of Quetiapine on AMPK Signalling via 5-HT7 Receptor: Exploring Pathophysiology of Clinical and Adverse Effects of Quetiapine International journal of molecular sciences 2022-08-14 [PMID: 36012369] (Simple Western, Rat)

Dilution used 1:300
Simple Western Rat
Fukuyama K, Motomura E, Okada M Brexpiprazole Reduces 5-HT7 Receptor Function on Astroglial Transmission Systems International journal of molecular sciences 2022-06-12 [PMID: 35743014] (Simple Western, WB, Rat) Simple Western, WB Rat
Fukuyama K, Motomura E, Shiroyama T, Okada M Impact of 5-HT7 receptor inverse agonism of lurasidone on monoaminergic tripartite synaptic transmission and pathophysiology of lower risk of weight gain Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022-02-24 [PMID: 35219120] (WB, Rat) WB Rat
Chaudhary P, Guragain D, Chang J, Kim J TPH1 and 5-HT7 Receptor Overexpression Leading to Gemcitabine-Resistance Requires Non-Canonical Permissive Action of EZH2 in Pancreatic Ductal Adenocarcinoma Cancers 2021-10-22 [PMID: 34771469] (WB, Human) WB Human
Okada M, Matsumoto R, Yamamoto Y, Fukuyama K Effects of Subchronic Administrations of Vortioxetine, Lurasidone, and Escitalopram on Thalamocortical Glutamatergic Transmission Associated with Serotonin 5-HT7 Receptor International journal of molecular sciences 2021-01-29 [PMID: 33572981] (WB, Rat) WB Rat
Tempio A, Niso M, Laera L et al. Mitochondrial Membranes of Human SH-SY5Y Neuroblastoma Cells Express Serotonin 5-HT7 Receptor International journal of molecular sciences 2020-12-17 [PMID: 33348850] (WB, Human) WB Human
Volpicelli F, Speranza L, Pulcrano S et al. The microRNA-29a Modulates Serotonin 5-HT7 Receptor Expression and Its Effects on Hippocampal Neuronal Morphology Mol. Neurobiol. 2019-07-10 [PMID: 31292861] (WB, Mouse) WB Mouse
Ito M, Komai K, Mise-Omata S Brain regulatory T cells suppress astrogliosis and potentiate neurological recovery Nature 2019-01-02 [PMID: 30602786] (FLOW, Mouse) FLOW Mouse
Wixey JA, Reinebrant HE, Chand KK, Buller KM. Disruption to the 5-HT7 Receptor Following Hypoxia-Ischemia in the Immature Rodent Brain Neurochem. Res. 2018-01-22 [PMID: 29357019] (WB, Rat) WB Rat
Show All 22 Publications.

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Gene Symbol HTR7