5-HT1A Antibody

Simple Western: 5-HT1A Antibody [NBP2-21590] - Detection of 5-HT1A by Simple WesternTM. Simple Western lane view shows lysates of Raji cells. A specific band was detected for 5-HT1A at approximately 60 kDa (as indicated) using 1:100 of Rabbit Anti-Human 5-HT1A Polyclonal Antibody (Catalog # NBP2-21590). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot: 5-HT1A Antibody [NBP2-21590] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: 5-HT1A Antibody [NBP2-21590] - DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Frozen: 5-HT1A Antibody [NBP2-21590] - Frozen Sectioned adult mouse hippocampus. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:250.Blue: Fluoroshield with DAPI.

Western Blot: 5-HT1A Antibody [NBP2-21590] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

• Reactivity: Hu, Mu, Rt, Pm, RM
• Applications: WB, Simple Western, ICC/IF, IHC-Fr
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

5-HT1A Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human 5-HT1A receptor. The exact sequence is proprietary.
• Predicted Species: Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTR1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Simple Western
  • Western Blot 1:500-1:3000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for 5-HT1A Antibody

• 5-HT1a receptor
• 5HT1A
• 5-HT1A
• 5-HT-1A
• 5-HT1A5-hydroxytryptamine receptor 1A
• 5-hydroxytryptamine (serotonin) receptor 1A
• ADRB2RL1
• ADRB2RL15HT1a
• ADRBRL1
• G protein coupled receptor
• G-21
• guanine nucleotide-binding regulatory protein-coupled receptor
• HTR1A
• PFMCD
• Serotonin receptor 1A

Background

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for 5-HT1A Antibody (NBP2-21590)(3)

Publications using NBP2-21590

• Okada M, Fukuyama K, Motomura E Dose-Dependent Biphasic Action of Quetiapine on AMPK Signalling via 5-HT7 Receptor: Exploring Pathophysiology of Clinical and Adverse Effects of Quetiapine International journal of molecular sciences 2022-08-14 [PMID: 36012369] (Simple Western, Rat)
  
  Details:
  Dilution used 1:300
• Fukuyama K, Motomura E, Okada M Brexpiprazole Reduces 5-HT7 Receptor Function on Astroglial Transmission Systems International journal of molecular sciences 2022-06-12 [PMID: 35743014] (WB, Rat)
• Fukuyama K, Motomura E, Shiroyama T, Okada M Impact of 5-HT7 receptor inverse agonism of lurasidone on monoaminergic tripartite synaptic transmission and pathophysiology of lower risk of weight gain Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022-02-24 [PMID: 35219120] (WB, Rat)

Bioinformatics

• Gene Symbol: HTR1A
• Uniprot:
  • P08908(Human)