SLC6A4/5-HTTLPR/Serotonin transporter Antibody - BSA Free

Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NBP3-05546] - Different tissue and cell lysates using NBP3-05546 at a dilution of 1:1,000 in green. Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. The prominent band at ~68kDa corresponds to SLC6A4 protein, and lower bands are likely are proteolytic products of SLC6A4. [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SLC6A4 cDNA. The bands between 50-75kDa mark in the transfected cells demonstrate overexpression of SLC6A4, and the band at about 150kDa presumably corresponds to an aggregated form of SLC6A4. The blot was probed with mouse mAb to GAPDH at a dilution of 1:5,000, in red, which reveals the single band at ~37kDa in both transfected and non-transfected cells. Note that HEK293 cells express endogenously (7).

Immunocytochemistry/Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NBP3-05546] - Immunofluorescent analysis of a rat brain section stained with SLC6A4/5-HTTLPR/Serotonin transporter Antibody in green and counterstained with chicken polyclonal antibody to tyrosine hydroxylase in red. The axons of serotoninergic neurons course throughout the section but are clearly distinct from the chatacholinergic processes and cell bodies revealed with the TH antibody. The blue stain reveals DNA in cell nuclei.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SLC6A4/5-HTTLPR/Serotonin transporter Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SLC6A4/5-HTTLPR/Serotonin transporter Antibody - BSA Free (NBP3-05546) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: KSITPETPTEIPCGDIRLNAV coupled to KLH
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC6A4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:2000-5000
  • Western Blot
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for SLC6A4/5-HTTLPR/Serotonin transporter Antibody - BSA Free

• 5HT transporter
• 5-HT Transporter
• 5-HTT
• 5HTTLPR
• 5-HTTLPR
• hSERT
• HTT5-hydroxytryptamine transporter
• Na+/Cl- dependent serotonin transporter
• OCD1
• Serotonin Transporter 1
• SERT
• SLC6A4
• sodium-dependent serotonin transporter
• solute carrier family 6 (neurotransmitter transporter, serotonin), member 4,5-HTTLPR
• Solute carrier family 6 member 4,5HTT

Background

Serotonin transporter encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC6A4