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5-HT6 Antibody

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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5-HT6 Antibody Summary

Immunogen
A synthetic peptide from aa region 400-450 of mouse 5HT6R conjugated to blue carrier protein was used as the antigen.
Specificity
Specific for 5HT6R receptor.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HTR6
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1: 300-1: 2000
  • Immunohistochemistry 1: 300-1: 2000
Publications
Read Publications using
NBP1-46557 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Rat reactivity reported in (PMID: 27161524).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for 5-HT6 Antibody

  • 5-HT6
  • 5-hydroxytryptamine (serotonin) receptor 6,5-HT65-HT6R
  • HTR6
  • Serotonin receptor 6,5-HT-6,5-hydroxytryptamine receptor 6

Background

5-HT6, a Serotonin Receptor, appears to regulate cholinergic neurotransmission in the brain rather than the expected interaction as a modulator of dopaminergic transmission. This interaction predicts a possible role for 5-HT6 Receptor antagonists in the treatment of learning and memory disorders. The 5-HT6 Receptor is abundant in limbic areas, which participate in the control of mood and emotion. Some antidepressants and antipsychotics are potent 5-HT6 Receptor antagonists, making this gene valuable for the pharmaceutical industry. 5-HT6 may be involved in the pathogenesis of mood disorders, schizophrenia, in Alzheimer's disease. A splice variant that has a 289bp deletion in the transmembrane region IV and the third intracellular loop has been isolated. 5-HT6 Receptor has been reported primarily in striatum of the brain. Expression has also been seen in stomach, spinal cord, and the lymphoid tissues of rats, including peripheral blood lymphocytes, spleen, and thymus. ESTs have been isolated from brain libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.
(15 Publications)
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5-HT2A Antibody
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Immunocytochemistry/Immunofluorescence: 5-HT2A Antibody [NBP2-26091] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: 5-HT2A Antibody [NBP2-26091] - Staining of paraffin embedded Human Cerebellum. Antibody at 2 ug/mL. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: 5-HT2A Antibody [NBP2-26091] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
5-HT1A Antibody
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Immunocytochemistry/Immunofluorescence: 5-HT1A Antibody [NBP2-21590] - DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: 5-HT1A Antibody [NBP2-21590] - Frozen Sectioned adult mouse hippocampus. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:250.Blue: Fluoroshield with DAPI.
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5-HT2C Antibody
(496214)
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A172 human glioblastoma cell line was stained with Mouse Anti-Human 5HT2C Monoclonal Antibody (Catalog # MAB5764, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
5HT2C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human 5HT2C Monoclonal Antibody (Catalog # MAB5764) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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5-HT4 Antibody
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Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LYVE1 in 1% milk/TBST. Reactivity was detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
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5-HT1B Antibody
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Calreticulin
Antibody
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SLC6A4/5-HTTLPR/Seroto ...
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Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.
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Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rabbit brain whole cell homogenate. Photo courtesy of product review by verified customer.
(2 Reviews)
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5-HT6 Antibody
NBP1-46557
5-HT6 Antibody
Species: Mu, Rt
Applications: WB, IHC

Publications for 5-HT6 Antibody (NBP1-46557)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol HTR6
Entrez
Uniprot