5-HT1B Antibody - BSA Free

Western Blot: 5-HT1B Antibody [NB100-56350] - Analysis of 5-HT1B in human brain lysate with anti-5-HT1BR pcAb (NB100-56350). A protein band of approximate molecular weight of 40-41kDa was detected.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

5-HT1B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit 5-HT1B Antibody - BSA Free (NB100-56350) is a polyclonal antibody validated for use in WB. Anti-5-HT1B Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 8-26 and 263-278 of rat 5-HT1BR (Uniprot# P28564)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTR1B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for 5-HT1B Antibody - BSA Free

• 5HT1B
• 5-HT1B
• 5-HT1B5-HT-1B
• 5HT1DB
• 5-HT1DB
• 5-hydroxytryptamine (serotonin) receptor 1B
• HTR1B
• HTR1D2
• HTR1D2,5-hydroxytryptamine receptor 1B
• HTR1DB
• S12
• S125-HT-1D-beta
• Serotonin 1D beta receptor
• Serotonin receptor 1B

Background

Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. Molecular cloning studies have identified 5 receptors belonging to the 5-HT1 subtypes. These receptors are negatively coupled to adenylyl cyclase. Radioligand binding studies suggest the presence of 5-HT1B (formerly known as 5-HT1DbR) sites in substantia nigra, dorsal subiculum, globus pallidus, and superior collicum. The in situ hybridization studies show abundance of 5-HT1BR mRNA in CA1 pyramidal cells of hippocampus, Purkinje cells of cerebellum, olfactory tubercle, subthalamic nucleus and somewhat lower levels in the caudate-putamen, cortical areas, thalamic and hypothalamic nuclei.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT1B Antibody (NB100-56350)(2)

Publications using NB100-56350

• Chen YH, Kuo TT, Wang V et Al. Serotonergic Regulation of Synaptic Dopamine Levels Mitigates L-DOPA-Induced Dyskinesia in a Mouse Model of Parkinson's Disease J Parkinsons Dis 2024-06-18 [PMID: 38905058]
• Ponte M, Ahern G, O'Connell P Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor Blood. [PMID: 17158224] (WB, Mouse)

Bioinformatics

• Gene Symbol: HTR1B
• Entrez:
  • 3351(Human)
  • 15551(Mouse)
  • 25075(Rat)
• Uniprot:
  • P28222()
  • P28334(Mouse)
  • P28564(Rat)