Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78984SS | |||
| Azide and BSA Free | NBP2-80695 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-94712SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4277-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74569SS | |||
| Azide and BSA Free | NBP2-80758 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80819 | |||
| BSA Free | NB100-507SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33315 | |||
| IHC-Prediluted | NBP2-44777 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2344-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2339-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF950-SP | |||
![Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Colocalisation of Dsg3 with E-cadherin-catenin complex. Co-IPs demonstrated that both endogenous and exogenous Dsg3 interacted with the E-cadherin-catenin complex in A431 stable lines (the empty vector control (V) and hDsg3.myc (D3)) as well as in HaCaTs. When pulled down with antibodies against the myc tag or with a mouse Dsg3 Ab 5H10 and blotted for E-cadherin and beta-catenin, both proteins were detected in D3 and, to a lesser extent, in V. Conversely, when pulled down with E-cadherin Ab HECD-1 and blotted for Dsg3 or the myc tag and Pg, these proteins were detected in both V and D3 cells. Note that this result also was confirmed in HaCaTs (HaC) where Dsg3 was expressed endogenously (far right blot). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014211), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Western-Blot-NBP1-78984-img0008.jpg "Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Colocalisation of Dsg3 with E-cadherin-catenin complex. Co-IPs demonstrated that both endogenous and exogenous Dsg3 interacted with the E-cadherin-catenin complex in A431 stable lines (the empty vector control (V) and hDsg3.myc (D3)) as well as in HaCaTs. When pulled down with antibodies against the myc tag or with a mouse Dsg3 Ab 5H10 and blotted for E-cadherin and beta-catenin, both proteins were detected in D3 and, to a lesser extent, in V. Conversely, when pulled down with E-cadherin Ab HECD-1 and blotted for Dsg3 or the myc tag and Pg, these proteins were detected in both V and D3 cells. Note that this result also was confirmed in HaCaTs (HaC) where Dsg3 was expressed endogenously (far right blot). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014211), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - The cultured primary mouse skin keratinocytes were stained at 1:150. The image shows the cytosolic staining. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Immunocytochemistry-NBP1-78984-img0007.jpg)
![Immunohistochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 on human skin cancer using NBP1-78984.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Immunohistochemistry-NBP1-78984-img0005.jpg)
![Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 expression in HaCat cell lysate using NBP1-78984.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Western-Blot-NBP1-78984-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Desmoglein 3 (5H10) antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Immunocytochemistry-Immunofluorescence-NBP1-78984-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Desmoglein 3 (5H10) antibody was tested in oral HNSCC cells fixed with 1% formaldehyde permeabilized in 0.1% Triton X-100.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Immunocytochemistry-Immunofluorescence-NBP1-78984-img0006.jpg)
![Western Blot: Senataxin Antibody [NBP1-94712] - Input: HeLa whole cell lysate. Beads without antibody IP control. IP: IP from HeLa lysate.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Western-Blot-NBP1-94712-img0002.jpg "Western Blot: Senataxin Antibody [NBP1-94712] - Input: HeLa whole cell lysate. Beads without antibody IP control. IP: IP from HeLa lysate.")
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Senataxin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94712-img0010.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (transverse section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very strong staining in the cytoplasm and the nuclei of the muscle cells. No signal was found in the perimysium and endomycium area (connective tissue) of the section.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunohistochemistry-Paraffin-NBP1-94712-img0007.jpg)
![Flow Cytometry: Senataxin Antibody [NBP1-94712] - An intracellular stain was performed on Jurkat cells and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .](http://images.novusbio.com/fullsize/Senataxin-Antibody-Flow-Cytometry-NBP1-94712-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - Senataxin antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94712-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - Senataxin immunofluorescence in fibroblasts. Image from verified customer review.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94712-img0004.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (verticle section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very strong staining in the cytoplasm and the nuclei of the muscle cells. No signal was found in the perimysium and endomycium (connective tissue) of the section.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunohistochemistry-Paraffin-NBP1-94712-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (MEM-263) [NB500-481] - analysis of CD44 in 4% paraformaldehyde fixed Caov-3 cells (adherent human ovarian cancer cells) using anti-Cd44 antibody. The primary antibody was used at a dilution of 1:1000 in 2% goat serum in PBS and incubated for 2 hours at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Immunocytochemistry-Immunofluorescence-NB500-481-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD44 Antibody (MEM-263) [NB500-481] - analysis of CD44 in 4% paraformaldehyde fixed Caov-3 cells (adherent human ovarian cancer cells) using anti-Cd44 antibody. The primary antibody was used at a dilution of 1:1000 in 2% goat serum in PBS and incubated for 2 hours at room temperature. Image from verified customer review.")
![CD44 Antibody (MEM-263) [NB500-481] - Analysis of CD44 in HeLa cells using anti-CD44 (MEM-263) purified; non-reducing conditions.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-NB500-481-img0009.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0003.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) APC conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0007.jpg)
![Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Expression of CD44 and CK18 in multiple clones of ovarian cancer cells. CD44+ cells also express CK18 as determined by western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jo/2011/620523/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Western-Blot-NB500-481-img0008.jpg)
![Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Western-Blot-NB500-481-img0002.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (MEM-263) PE conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0004.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) FITC conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0005.jpg)
![Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.](http://images.novusbio.com/fullsize/HAP1-Antibody-1B6-Western-Blot-NB110-74569-img0006.jpg "Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.")
![Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569] - Staining of rat hypothalamus.](http://images.novusbio.com/fullsize/HAP1-Antibody-1B6-Immunohistochemistry-NB110-74569-img0004.jpg)
![Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/HAP1-Antibody-1B6-Flow-Cytometry-NB110-74569-img0007.jpg)
![Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Detection of HAP1 in mouse brain lysate.](http://images.novusbio.com/fullsize/HAP1-Antibody-1B6-Western-Blot-NB110-74569-img0005.jpg)
![Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0013.jpg "Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - HeLa cells stained with anti-APE Antibody (NB100-116, Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0014.jpg)
![Immunohistochemistry: APE Antibody Pack [NB100-909] - Immunohistochemical staining of APE1 using NB100-116. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear APE1 expression in invasive breast cancer. (magnification x400). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0099528) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunohistochemistry-NB100-909-img0015.jpg)
![Western Blot: APE Antibody Pack [NB100-909] - Analysis of APE1 using NB100-101 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - Detection of APE1 using NB100-101 in HeLa cells. Image from verified customer review.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0012.jpg)
![Immunohistochemistry: APE1 Antibody Pack [NB100-909] - Staining of APE-ref-1 in prostate cancer, using NB100-101.](http://images.novusbio.com/fullsize/APE1-Antibody-Pack-Immunohistochemistry-NB100-909-img0005.jpg)
![Simple Western: APE Antibody Pack [NB100-909] - Lane view shows a specific band for APE1 using NB100-101 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Simple-Western-NB100-909-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: APE Antibody Pack [NB100-909] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1 (using NB100-116), p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Knockdown-Validated-NB100-909-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW128) at 5 ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Immunocytochemistry-Immunofluorescence-NB100-507-img0005.jpg "Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW128) at 5 ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.")
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Detection of p300 in a HeLa nuclear extract using NB100-507 (1:250). ECL: 15 sec exposure.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0003.jpg)
![Flow (Intracellular): KAT3B/p300 Antibody (RW128) [NB100-507] - An intracellular stain was performed on THP-1 cells with KAT3B/p300 (RW128) antibody NB100-507 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Flow-Intracellular-NB100-507-img0004.jpg)
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Kinase active Src is present in the nuclei of Panc-1 cells in association with p300. Subcellular fractionation and localization of Src and p300 in Panc-1 cells. Cell lysates of equal total protein or Panc-1 subcellular fractions were subjected to immunoblotting analysis for Src, pSrc, p300, tubulin (cytoskeletal proteins), GAPDH (soluble cytoplasmic proteins), calnexin (endoplasmic reticulum), grim19 (mitochondria), histone H3 (chromatin), and lamin A/C (nuclear lamina). Image collected and cropped by Citeab from the following publication (A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B/p300 Antibody (RW128) NB100-507 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Immunocytochemistry-Immunofluorescence-NB100-507-img0009.jpg)
![Flow Cytometry: KAT3B/p300 Antibody (RW128) [NB100-507] - An intracellular stain was performed on Raw264.7 cells with KAT3B/p300 Antibody (RW128) NB100-507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Flow-Cytometry-NB100-507-img0010.jpg)
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Kinase active Src is present in the nuclei of Panc-1 cells in association with p300. Src and p300 co-association in Panc-1 cells. Immunoblotting analysis of Src or p300 immunecomplexes from Panc-1 nuclear isolates probing for Src or p300, or IgG control. Image collected and cropped by Citeab from the following publication (A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0006.jpg)
![Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (100/D5).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Western-Blot-NBP2-15200-img0006.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (100/D5) [NBP2-15200] - Formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 Ab (100/D5). Note nuclear membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Immunohistochemistry-Paraffin-NBP2-15200-img0005.jpg)
![Flow Cytometry: Bcl-2 Antibody (100/D5) [NBP2-15200] - Intracellular staining of Bcl-2 in fixed and permeabilized Jurkat cells using Bcl-2 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Flow-Cytometry-NBP2-15200-img0002.jpg)
![Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - analysis of Bcl-2 in human skin using Bcl-2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Western-Blot-NBP2-15200-img0004.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - HPV-16E7 and -58E7 upregulated CIP2A mRNA and protein levels in PHKs(A) Western blot analysis of CIP2A protein level in PHKs expressing HPV-16E7, -58E7, -6E7. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2867), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Western-Blot-NB100-68264-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: CIP2A Antibody [NB100-68264] - HPV-16E7 and -58E7 upregulated CIP2A mRNA and protein levels in PHKs(A) Western blot analysis of CIP2A protein level in PHKs expressing HPV-16E7, -58E7, -6E7. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2867), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CIP2A Antibody [NB100-68264] - Human colon carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize/CIP2A-Antibody-Immunohistochemistry-Paraffin-NB100-68264-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CIP2A Antibody [NB100-68264] - Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Western-Blot-NB100-68264-img0003.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CIP2A Antibody [NB100-68264] - Detection of Human CIP2A on HeLa whole cell lysate using NB100-68264. CIP2A was also immunoprecipitated by rabbit anti-CIP2A antibody NB100-68263.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Western-Blot-NB100-68264-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CIP2A Antibody [NB100-68264] - Knockdown of CIP2A inhibited cell proliferation and DNA synthesis of HPV-16E7-expressing cells with CIP2A siRNA for 48 hr. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2867), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CIP2A-Antibody-Knockdown-Validated-NB100-68264-img0004.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - HPV-16E7 & -58E7 upregulated CIP2A mRNA & protein levels in PHKs(A) Western blot analysis of CIP2A protein level in PHKs expressing HPV-16E7, -58E7, -6E7; & (B) Quantification. (C) qRT-PCR analysis of CIP2A mRNA level in PHKs expressing HPV-16E7, -58E7, -6E7. (D) Western blot analysis of CIP2A protein level in PHKs expressing HPV-16E7 & 16E7 mutants L22A & C24G. Babe, vector control. *, P < 0.05; **, P < 0.01; & ***, P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25650660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415291953.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Silencing CIP2A caused decreased Cdk1 & Cdk2 proteins in 16E6‐expressing cells. A, Western blot analysis of CIP2A, Cdk4, Cdk6, cyclin D1, Cdk1, Cdk2, cyclin B1, cyclin A2 & cyclin E1 protein levels in cells expressing HPV‐16E6 transfected with CIP2A siRNA & then treated with PBS or 10 μg/mL bleomycin for 24 h. A representative of 3 independent experiments is shown. B, Quantification of all cell cycle‐related proteins. Data from 3 experiments are summarized. C, Relative mRNA levels of all cell cycle‐related genes determined by qRT‐PCR. Data from 3 experiments are summarized. *, P < .05; **, P < .01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415395935.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Inhibition of Cdk1 & Cdk2 by CIP2A knockdown in cervical cancer SiHa cells caused G1 arrest. A, Western blot analysis of 16E6, p53 & CIP2A after HPV‐16E6 knockdown in cervical cancer SiHa cells. B, Protein expression of CIP2A, B‐Myb, Cdk1 & Cdk2 in SiHa cells after CIP2A knockdown. Data from a representative of 3 experiments are shown. C, Flow cytometric analysis of SiHa cells with CIP2A knockdown treated with PBS or bleomycin. A representative flow cytometry of 3 independent experiments is shown. D, Quantification of percentages G1 phase cells. Data from 3 experiments are summarized. *, P < .05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415334911.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Knockdown of CIP2A inhibited cell proliferation & DNA synthesis of HPV-16E7-expressing cells(A) Western blot analysis of protein level of 16E7 & CIP2A in RPE1-16E7 cells & (B) with CIP2A siRNA for 48 hr. (C) CCK8 assay of cell proliferation of RPE1-16E7 cells with CIP2A siRNA. (D) Flow cytometry of cells with CIP2A siRNA & labeled with BrdU for 2 hr, then stained with PI & BrdU; & (E), Quantification. Babe, vector control. **, P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25650660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-31020241534369.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Inhibition of Cdk1 & Cdk2 by CIP2A knockdown in cervical cancer SiHa cells caused G1 arrest. A, Western blot analysis of 16E6, p53 & CIP2A after HPV‐16E6 knockdown in cervical cancer SiHa cells. B, Protein expression of CIP2A, B‐Myb, Cdk1 & Cdk2 in SiHa cells after CIP2A knockdown. Data from a representative of 3 experiments are shown. C, Flow cytometric analysis of SiHa cells with CIP2A knockdown treated with PBS or bleomycin. A representative flow cytometry of 3 independent experiments is shown. D, Quantification of percentages G1 phase cells. Data from 3 experiments are summarized. *, P < .05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415395929.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Inhibition of CIP2A by siRNA impeded cell viability & DNA synthesis in HPV‐16E6–expressing cells. A, Elevated expression of CIP2A protein in 16E6‐expressing RPE1 cells. B, Western blot analysis of CIP2A & p53 proteins after transfection with scrambled siRNA (siCon) or CIP2A siRNA (siCIP2A) for 48 h. A representative of 3 independent experiments is shown. C, Cell viability assay of RPE1‐16E6 cells with CIP2A knockdown. D, Representative flow cytometry of BrdU staining profiles is shown. E, The mean & SD of BrdU‐positive cells from 3 experiments are summarized. **, P < .01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415384150.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - CIP2A siRNA knockdown caused G1 arrest & decreased Cdk1 & Cdk2 protein levels in E7-expressing cells(A) Flow cytometry of cells expressing 16E7 transfected with CIP2A siRNA for 48 hr, treated with DMSO control or bleomycin (10 μg/mL) for 24 hr, then stained with PI. G1, S & G2 phases are indicated. (B) Western blot analysis of Cdk1, Cdk2, Cyclin A2, Cdk4, Cdk6, Cyclin D1 protein levels in cells expressing HPV-16E7 transfected with CIP2A siRNA. Babe, vector control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25650660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-31020241534317.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Knockdown of CIP2A inhibited cell proliferation & DNA synthesis of HPV-16E7-expressing cells(A) Western blot analysis of protein level of 16E7 & CIP2A in RPE1-16E7 cells & (B) with CIP2A siRNA for 48 hr. (C) CCK8 assay of cell proliferation of RPE1-16E7 cells with CIP2A siRNA. (D) Flow cytometry of cells with CIP2A siRNA & labeled with BrdU for 2 hr, then stained with PI & BrdU; & (E), Quantification. Babe, vector control. **, P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25650660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-3102024153564.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Inhibition of CIP2A by siRNA impeded cell viability & DNA synthesis in HPV‐16E6–expressing cells. A, Elevated expression of CIP2A protein in 16E6‐expressing RPE1 cells. B, Western blot analysis of CIP2A & p53 proteins after transfection with scrambled siRNA (siCon) or CIP2A siRNA (siCIP2A) for 48 h. A representative of 3 independent experiments is shown. C, Cell viability assay of RPE1‐16E6 cells with CIP2A knockdown. D, Representative flow cytometry of BrdU staining profiles is shown. E, The mean & SD of BrdU‐positive cells from 3 experiments are summarized. **, P < .01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415284523.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Regulation of Cdk1 & Cdk2 by CIP2A is dependent on B‐Myb rather than c‐Myc. A, Western blot analysis of CIP2A, c‐Myc, phospho‐S62‐Myc & B‐Myb protein levels in 16E6‐expressing cells after CIP2A knockdown. beta ‐Tubulin was used as a loading control. B, Protein levels of B‐Myb, c‐Myc & phospho‐S62‐Myc in 16E6‐expressing PHKs & (C) RPE1 cells. D, Protein levels of B‐Myb, Cdk1 & Cdk2, CIP2A & p53 in 16E6‐expressing cells after B‐Myb knockdown with siRNA. Data from a representative of 3 experiments are shown. E, Knockdown of B‐Myb down‐regulates Cdk1 & Cdk2 luciferase reporter activities. RPE1 cells were cotransfected with the Cdk1 or Cdk2 promoter‐luciferase constructs & renilla luciferase control plasmid together with B‐Myb siRNA plasmid. Cells were harvested after 48 h, & lysates were assayed for luciferase activity. F, Flow cytometric analysis of 16E6‐expressing cells transfected with B‐Myb siRNA treated with PBS or bleomycin. G1, S & G2 phases are indicated. A representative flow cytometry of 3 independent experiments is shown. G, Quantification of percentages G1 phase cells. Data from 3 experiments are summarized. H, Western blot analysis of B‐Myb, Cdk1 & Cdk2 in B‐Myb–overexpressing CIP2A knockdown cells. Data from a representative of 3 experiments are shown. *, P < .05; ***, P < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-31020241612638.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Induction of CIP2A mRNA & protein expression by HPV‐16E6 in PHKs. A, mRNA expression of HPV‐16E6 in PHKs expressing 16E6 & F2V using beta ‐actin as a loading control. B, Protein levels of HPV‐16E6, p53 & p21 in PHKs expressing 16E6 & F2V. Expression of GAPDH was used as a loading control. A representative of 2 independent experiments is shown. C, HPV‐16E6 expression leads to increased protein expression of CIP2A in PHKs. Data from a representative of 3 experiments are shown. D, Data from 3 experiments are summarized. E, Relative CIP2A mRNA expression was determined by qRT‐PCR in the above cells. Data from 3 experiments are summarized. The mean & standard deviation (SD) of 3 independent experiments are shown. Babe, pBabe‐puromycin vector. *, P < .05; **, P < .01; & ***, P < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-3102024161263.jpg)
![Western Blot: CIP2A Antibody [NB100-68264] - Regulation of Cdk1 & Cdk2 by CIP2A is dependent on B‐Myb rather than c‐Myc. A, Western blot analysis of CIP2A, c‐Myc, phospho‐S62‐Myc & B‐Myb protein levels in 16E6‐expressing cells after CIP2A knockdown. beta ‐Tubulin was used as a loading control. B, Protein levels of B‐Myb, c‐Myc & phospho‐S62‐Myc in 16E6‐expressing PHKs & (C) RPE1 cells. D, Protein levels of B‐Myb, Cdk1 & Cdk2, CIP2A & p53 in 16E6‐expressing cells after B‐Myb knockdown with siRNA. Data from a representative of 3 experiments are shown. E, Knockdown of B‐Myb down‐regulates Cdk1 & Cdk2 luciferase reporter activities. RPE1 cells were cotransfected with the Cdk1 or Cdk2 promoter‐luciferase constructs & renilla luciferase control plasmid together with B‐Myb siRNA plasmid. Cells were harvested after 48 h, & lysates were assayed for luciferase activity. F, Flow cytometric analysis of 16E6‐expressing cells transfected with B‐Myb siRNA treated with PBS or bleomycin. G1, S & G2 phases are indicated. A representative flow cytometry of 3 independent experiments is shown. G, Quantification of percentages G1 phase cells. Data from 3 experiments are summarized. H, Western blot analysis of B‐Myb, Cdk1 & Cdk2 in B‐Myb–overexpressing CIP2A knockdown cells. Data from a representative of 3 experiments are shown. *, P < .05; ***, P < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29893470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68264_rabbit-polyclonal-cip2a-antibody-310202415535140.jpg)
