CIP2A Antibody

Images

 
Western Blot: CIP2A Antibody [NB100-68264] - Detection of Human CIP2A on HeLa whole cell lysate using NB100-68264. CIP2A was also immunoprecipitated by rabbit anti-CIP2A antibody NB100-68263.
Immunohistochemistry-Paraffin: CIP2A Antibody [NB100-68264] - Human colon carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).
Western Blot: CIP2A Antibody [NB100-68264] - Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

CIP2A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 853 and 903 of human cancerous inhibitor of PP2A using the numbering given in entry NP_065941.1 (GeneID 57650).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIAA1524
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Immunohistochemistry 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
CIP2A Lysate (NBL1-12266)
Publications
Read Publications using
NB100-68264 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CIP2A Antibody

  • Cancerous inhibitor of PP2A
  • CIP2A
  • FLJ12850
  • KIAA1524
  • MGC163436
  • p90 autoantigen
  • p90
  • protein CIP2A

Background

CIP2A (cancerous inhibitor of PP2A) was originally identified as p90, a cytoplasmic autoantigen found in the serum from a cancer patient. It was later described as a protein that interacted with the c-myc oncogene and functioned as an inhibitor of protein phosphatase 2A (PP2A) activity. Inhibition of PP2A phosphatase activity by CIP2A prevents c-myc phosphorylation and counteracts c-myc proteolytic degradation, thereby stabilizing c-myc and promoting cell growth and transformation. CIP2A is found to be over expressed in some human malignancies and is thus classified as an oncogene that may be an important contributor to tumor formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CIP2A Antibody (NB100-68264)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-P
(1)
WB
(5)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-68264 Applications Species
Zhang W, Chen H, Chen Y et al. Cancerous inhibitor of protein phosphatase 2A contributes to human papillomavirus oncoprotein E7-induced cell proliferation via E2F1. Oncotarget 2015 Mar 10 [PMID: 25650660] (WB) WB
Guo Z, Liu D, Su Z. CIP2A mediates prostate cancer progression via the c-MYC signaling pathway Tumor Biology. 2015 Jan 06 [PMID: 25560487] (IHC-P, WB, Human) IHC-P, WB Human
Wu Y, Gu TT, Zheng PS. CIP2A cooperates with H-Ras to promote epithelial-mesenchymal transition in cervical-cancer progression. Cancer Lett 2015 Jan 28 [PMID: 25458953]
Wang J, Huang T, Sun J et al. CIP2A is overexpressed and involved in the pathogenesis of chronic myelocytic leukemia by interacting with breakpoint cluster region-Abelson leukemia virus. Med. Oncol. 2014 Aug 01 [PMID: 25023053] (WB, Human)

Details:
CIP2A antibody used for WB in human samples. Fig. 1B shows the blot of CIP2A expression in bone marrow mononuclear cells from CML-CP patients (lanes 1-7) and healthy control (lane C). Fig. 2A and Fig 3A/B shows the CIP2A blots from K562 cells transfected CIP2A-specific or control siRNA. Fig 3C shows its levels in cells treated with CIP2A expression plasmid.
WB Human
Johnson Mahlon D, Reeder Jay E, O'Connell Mary et al. CIP2A and PP2A in human leptomeninges, arachnoid granulations and meningiomas. J Clin Neurosci. 2014 Jul 08 [PMID: 25012485] (Human) Human
Kim JS, Kim EJ, Oh JS, Park IC, Hwang SG. CIP2A modulates cell cycle progression in human cancer cells by regulating the stability and activity of PLK1. Cancer Res. 2013 Aug 27 [PMID: 23983103] (IHC, Human) IHC Human
Xue Y, Wu G, Wang X et al. CIP2A is a predictor of survival and a novel therapeutic target in bladder urothelial cell carcinoma Med Oncol 2013 Mar [PMID: 23275123] (WB, IHC, Human) WB, IHC Human
Pallai R, Bhaskar A, Sodi V, Rice LM. Ets1 and Elk1 transcription factors regulate cancerous inhibitor of protein phosphatase 2A expression in cervical and endometrial carcinoma cells Transcription 2012 Nov 1 [PMID: 23117818]
Cristobal I, Garcia-Orti L, Cirauqui C et al. PP2A impaired activity is a common event in acute myeloid leukemia and its activation by forskolin has a potent anti-leukemic effect Leukemia 2011 Apr [PMID: 21233840] (WB, Human) WB Human

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on CIP2A.

CIP2A - A lean, mean, oncoprotein machine
The CIP2A protein was originally identified as p90, a cytoplasmic auto-antigen from the serum of a cancer patient. It was later found to inhibit protein phosphatase 2A (PP2A) activity as well as interact with c-myc. CIP2A's inhibitory activity blocks...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

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Bioinformatics

Gene Symbol KIAA1524
Entrez
Uniprot