Desmoglein-3 Antibody (5H10) - Azide and BSA Free

Western Blot: Desmoglein-3 Antibody (5H10) - Azide and BSA Free [NBP2-80695] - Colocalisation of Dsg3 with E-cadherin-catenin complex. Co-IPs demonstrated that both endogenous and exogenous Dsg3 interacted with the E-cadherin-catenin complex in A431 stable lines (the empty vector control (V) and hDsg3.myc (D3)) as well as in HaCaTs. When pulled down with antibodies against the myc tag or with a mouse Dsg3 Ab 5H10 and blotted for E-cadherin and beta-catenin, both proteins were detected in D3 and, to a lesser extent, in V. Conversely, when pulled down with E-cadherin Ab HECD-1 and blotted for Dsg3 or the myc tag and Pg, these proteins were detected in both V and D3 cells. Note that this result also was confirmed in HaCaTs (HaC) where Dsg3 was expressed endogenously (far right blot). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014211), licensed under a CC-BY license. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) - Azide and BSA Free [NBP2-80695] - The cultured primary mouse skin keratinocytes were stained at 1:150. The image shows the cytosolic staining. This image was submitted via customer review. Image from the standard format of this antibody.

Immunohistochemistry: Desmoglein-3 Antibody (5H10) - Azide and BSA Free [NBP2-80695] - Analysis of Desmoglein 3 on human skin cancer using NBP1-78984. Image from the standard format of this antibody.

Western Blot: Desmoglein-3 Antibody (5H10) - Azide and BSA Free [NBP2-80695] - Analysis of Desmoglein 3 expression in HaCat cell lysate using NBP1-78984. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) - Azide and BSA Free [NBP2-80695] - Desmoglein 3 (5H10) antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with Dapi (blue). Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) - Azide and BSA Free [NBP2-80695] - Desmoglein 3 (5H10) antibody was tested in oral HNSCC cells fixed with 1% formaldehyde permeabilized in 0.1% Triton X-100. Image from the standard format of this antibody.

• Reactivity: Hu, Mu
• Applications: WB, DB, ICC/IF, IHC, IP
• Clone: 5H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Desmoglein-3 Antibody (5H10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Desmoglein-3 Antibody (5H10) - Azide and BSA Free (NBP1-78984) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The extracellular domain of human Desmoglein 3 [Swiss-Prot# P32926]
• Localization: Cell membrane; Single-pass type I membrane protein. Cell junction > desmosome
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DSG3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot reported in scientific literature (PMID 15588331)
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: In Western blot, a band can be seen at approximately 130 kDa.
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Desmoglein-3 Antibody (5H10) - Azide and BSA Free

• Cadherin family member 6
• CDHF6
• CDHF6130-kD pemphigus vulgaris antigen
• desmoglein 3 (pemphigus vulgaris antigen)
• desmoglein 3
• Desmoglein3
• Desmoglein-3
• DKFZp686P23184,130 kDa pemphigus vulgaris antigen
• DSG3
• pemphigus vulgaris antigen
• PVA

Background

Desmoglein 3 (Dsg3) is a member of sub-family the desmoglein (DSGs) and it mainly serves as adhesion component of intercellular desmosome junctions. DSGs serve essential part of the core complex of desmosomes, and together with desmocollin (DSCs), they represents transmembrane proteins which mediate adhesion by transinteracting with cadherins of the opposing cell. Homophilic (e.g., DSG3-DSG3) as well as heterophilic (e.g., DSC3-DSG1) transinteraction have been reported for these proteins and within the desmosome, DSGs/DSCs are anchored to the intracellular plaque proteins plakoglobin, plakophilins, and desmoplakin, the latter of which mediates connection to the intermediate filament cytoskeleton. Desmoglein 3 is predominately expressed in stratified squamous epithelia including epidermis, tongue, tonsil, esophagus and carcinomas. Desmoglein 3 has been characterized as an auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease, wherein the binding of anti-desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DSG3