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APE Antibody Pack

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Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunocytochemistry/ Immunofluorescence: APE Antibody Pack [NB100-909] - HeLa cells stained with anti-APE Antibody (NB100-116, Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei ...read more
Immunohistochemistry: APE Antibody Pack [NB100-909] - Immunohistochemical staining of APE1 using NB100-116. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear ...read more
Western Blot: APE Antibody Pack [NB100-909] - Analysis of APE1 using NB100-101 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7.
Immunocytochemistry/ Immunofluorescence: APE Antibody Pack [NB100-909] - Detection of APE1 using NB100-101 in HeLa cells. Image from verified customer review.
Immunohistochemistry: APE1 Antibody Pack [NB100-909] - Staining of APE-ref-1 in prostate cancer, using NB100-101.
Simple Western: APE Antibody Pack [NB100-909] - Lane view shows a specific band for APE1 using NB100-101 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Genetic Strategies: Knockdown Validated: APE Antibody Pack [NB100-909] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IP, ChIP
Validated by:
     

Genetic Strategies

   

Order Details

APE Antibody Pack Summary

Description
This pack contains 1 vial each of: NB100-116 (0.1 mL), NB100-101 (0.1 mL).
Immunogen
See individual datasheets
Specificity
These antibodies are specific for APE1.
Gene
APEX1

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Simple Western
  • Western Blot
Application Notes
See individual datasheets of components for their validated applications
Antagonist
Publications
Read Publications using
NB100-909 in the following applications:

  • WB
    1 publication

Reactivity Notes

See individual datasheets of components for their validated species

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
See individual datasheets
Preservative
0.05% Sodium Azide

Kit Components

Alternate Names for APE Antibody Pack

  • AP endonuclease 1
  • APE
  • APE1
  • APE-1
  • APEAPEX nuclease (multifunctional DNA repair enzyme)
  • APENAP endonuclease class I
  • APEX deoxyribonuclease (apurinic or apyrimidinic)
  • APEX nuclease (multifunctional DNA repair enzyme) 1
  • APEX nuclease
  • APEX1
  • Apurinic-apyrimidinic endonuclease 1
  • APXAP lyase
  • DNA-(apurinic or apyrimidinic site) lyase
  • EC 3.1
  • EC 4.2.99.18
  • HAP1apurinic/apyrimidinic (abasic) endonuclease
  • multifunctional DNA repair enzyme
  • protein REF-1
  • redox factor 1
  • Redox factor-1
  • REF1 apurinic/apyrimidinic exonuclease
  • REF-1

Background

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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Publications for APE Antibody Pack (NB100-909)(4)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for APE Antibody Pack (NB100-909) (0)

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Bioinformatics

Gene Symbol APEX1