HAP1 Antibody (1B6) [CoraFluor™ 1]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: 1B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

HAP1 Antibody (1B6) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Fusion protein from rat HAP1. [UniProt# P54256]
• Specificity: This is specific for isoforms A and B.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for HAP1 Antibody (1B6) [CoraFluor™ 1]

• HAP2huntingtin-associated protein 2
• hHLP1
• HIP5
• HLP1
• HLPHAP-1
• huntingtin-associated protein 1
• Neuroan 1

Background

Huntington's disease is an autosomal dominant neurodegenerative disorder caused by an expanded polyglutamine repeat region in the huntingtin gene. Huntingtin-associated protein 1 (HAP1) is a huntingtin associated protein that shows neuronal localization and moves with huntingtin in nerve fibers. The ability of HAP1 to bind to huntingtin is enhanced by the expanded polyglutamine repeat region that is typical of the mutant form of the protein associated with Huntington's disease.

Defects with HAP1 can lead to Huntington's disease, and HAP1 antibodies are therefore useful tools for Huntington's disease research and neuroscience studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAP1