Coagulation Factor III/Tissue Factor Antibody [Unconjugated]

Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Coagulation Factor III/Tissue Factor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Coagulation Factor III/Tissue Factor at approximately 48 kDa (as indicated). GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Coagulation Factor III/Tissue Factor Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III/Tissue Factor
  Gly34-Glu251
  Accession # P13726
• Specificity: Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: F3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor III/Tissue Factor Antibody [Unconjugated]

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1-32) and the mature chain (residues 33-295). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-295) (3-6).

1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
5. Morrissey, J.H. et al. (1987) Cell 50:129.
6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coagulation Factor III/Tissue Factor Antibody (AF2339)(7)

Publications using AF2339

• C Mwase, TN Phung, MJ O'Sullivan, JA Mitchel, M De Marzio, A K?l?ç, ST Weiss, JJ Fredberg, JA Park Mechanical Compression of Human Airway Epithelial Cells Induces Release of Extracellular Vesicles Containing Tenascin C Cells, 2022-01-13;11(2):. 2022-01-13 [PMID: 35053372] (Western Blot, Human)
• TLR Humphries, K Shen, A Iyer, DW Johnson, GC Gobe, D Nikolic-Pa, DP Fairlie, DA Vesey PAR2-Induced Tissue Factor Synthesis by Primary Cultures of Human Kidney Tubular Epithelial Cells Is Modified by Glucose Availability International Journal of Molecular Sciences, 2021-07-14;22(14):. 2021-07-14 [PMID: 34299151] (Western Blot, Human)
• A Iyer, TLR Humphries, EP Owens, KN Zhao, PP Masci, DW Johnson, D Nikolic-Pa, GC Gobe, DP Fairlie, DA Vesey PAR2 Activation on Human Kidney Tubular Epithelial Cells Induces Tissue Factor Synthesis, That Enhances Blood Clotting Frontiers in Physiology, 2021-03-10;12(0):615428. 2021-03-10 [PMID: 33776786] (Western Blot, Human)
• LM Gockel, JM Ponert, S Schwarz, M Schlesinge, G Bendas The Low Molecular Weight Heparin Tinzaparin Attenuates Platelet Activation in Terms of Metastatic Niche Formation by Coagulation-Dependent and Independent Pathways Molecules, 2018-10-24;23(11):. 2018-10-24 [PMID: 30356007] (Flow Cytometry, Human)
• Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation Sci Rep, 2016-05-04;6(0):25171. 2016-05-04 [PMID: 27142573] (IHC-P, Human)
• Conroy AL, Liles WC, Molyneux ME Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi. PLoS ONE, 2011-12-12;6(12):e28540. 2011-12-12 [PMID: 22174834] (ELISA Development, Human)
• Kelly OG, Chan MY, Martinson LA Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat. Biotechnol., 2011-07-31;29(8):750-6. 2011-07-31 [PMID: 21804561] (IHC-Fr, Human)

Reviews for Coagulation Factor III/Tissue Factor Antibody (AF2339) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/06/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22828416
• Species: Human

reviewed by: Verified Customer IF Human 01/06/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23468018
• Species: Human

FAQs for Coagulation Factor III/Tissue Factor Antibody (AF2339). (Showing 1 - 1 of 1 FAQs).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: F3
• Uniprot:
  • P13726()