Coagulation Factor III/Tissue Factor Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Coagulation Factor III/Tissue Factor Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III/Tissue Factor
Gly34-Glu251
Accession # P13726
Specificity
Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
F3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
Positive Control
Coagulation Factor III/Tissue Factor Lysate (NBP2-64632)
Reviewed Applications
Read 2 Reviews rated 4
using
AF2339 in the following applications:

Publications
Read Publications using
AF2339 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor III/Tissue Factor Antibody

  • CD142 antigen
  • CD142
  • coagulation factor III (thromboplastin, tissue factor)
  • Coagulation Factor III
  • F3
  • FLJ17960
  • TF
  • TFA
  • Thromboplastin
  • Tissue Factor

Background

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1-32) and the mature chain (residues 33-295). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-295) (3-6).

  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
  3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
  4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
  5. Morrissey, J.H. et al. (1987) Cell 50:129.
  6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Coagulation Factor III/Tissue Factor Antibody (AF2339)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ELISA Development, IHC Frozen, IHC Paraffin-embedded.


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ELISA Development
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(1)
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Human
(3)
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Reviews for Coagulation Factor III/Tissue Factor Antibody (AF2339) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF2339:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22828416
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22828416

Details

Detection NotesSee PMID 22828416

Comments

CommentsPublished: See PMID 22828416
 
reviewed by:
Anonymous
IF Human 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23468018
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23468018

Details

Detection NotesSee PMID 23468018

Comments

CommentsPublished: See PMID 23468018

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Coagulation Factor III/Tissue Factor Antibody (AF2339). (Showing 1 - 1 of 1 FAQs).

  1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
    • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Coagulation Factor III/Tissue Factor Antibody (AF2339)

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Diseases for Coagulation Factor III/Tissue Factor Antibody (AF2339)

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Pathways for Coagulation Factor III/Tissue Factor Antibody (AF2339)

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PTMs for Coagulation Factor III/Tissue Factor Antibody (AF2339)

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

 
Anonymous
01/06/2015
Application: WB
Species: Human

 
Anonymous
01/06/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol F3
Entrez
Uniprot