HAP1 Antibody (1B6)

Images

 
Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.
Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569] - Staining of rat hypothalamus.
Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more
Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Detection of HAP1 in mouse brain lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
1B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HAP1 Antibody (1B6) Summary

Immunogen
Fusion protein from rat HAP1. [UniProt# P54256]
Specificity
This is specific for isoforms A and B.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HAP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0 ug/ml
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 0.5-1.0 ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0 ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
This HAP1 antibody is useful for Immunohistochemistry frozen and paraffin embedded sections, Western blot, Flow Cytometry and Immunoprecipitation. In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Positive Control
Brain Lysate (NB820-59705)
Publications
Read Publications using
NB110-74569 in the following applications:

  • 1 publication
  • IP
    2 publications
  • WB
    5 publications

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HAP1 Antibody (1B6)

  • HAP2huntingtin-associated protein 2
  • hHLP1
  • HIP5
  • HLP1
  • HLPHAP-1
  • huntingtin-associated protein 1
  • Neuroan 1

Background

HAP1 (Huntingtin-associated protein 1) is a cytoplasmic protein that interacts with several proteins such as Huntingtin, p150Glued, KLC, TrkA, TATA-binding protein, proBDNF etc. and plays a key role in microtubule-dependent transport, vesicular trafficking, nerve growth, transcriptional regulation and signal transduction. HAP1 was originally discovered as huntingtin interacting neuronal protein (htt), the Huntington's disease protein, however, unlike htt (expressed ubiquitously throughout body), HAP1 is enriched in brain with abundant expression in the hypothalamus, amygdala, brain stem etc. HAP1 has been associated with microtubule-dependent transporters dynactin p150 and kinesin light chain, as well as with transport of various vesicular proteins or membrane receptors, including BDNF-containing vesicles, EGFR, type 1 inositol (1,4,5)-triphosphate receptor, gamma -aminobutyric acid, type A, receptor, and TRKA. Hap1 is also implicated in microtubule-dependent trafficking in neurons, vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for HAP1 Antibody (NB110-74569)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(2)
WB
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(5)
Rat
(3)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB110-74569 Applications Species
Xiang J, Yang S, Xin N et al. DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome Proc. Natl. Acad. Sci. U.S.A Jan 30 2017 [PMID: 28137862] (IP, Mouse) IP Mouse
Corradini E, Burgers PP, Plank M et al. Huntingtin associated protein (HAP1) is a cGMP-dependent kinase anchoring protein (GKAP), specific for the cGMP-dependent protein kinase Ibeta isoform J. Biol. Chem. 2015 Feb 04 [PMID: 25653285] (WB, Human)

Details:
HAP1 antibody used for WB analysis of totall cell lysates as well as the pulldowns performed in COS-7 cells which were co-transfected with HAP1 alone or with either PKG I alpha or PKG I beta (FIGURE 2). WB also performed on lysates and proteins pulled down in COS-7 cells co-expressing PKG I beta-GFP plasmid with HAP1-DSRed construct (Control), HAP1-DSRed bearing the double mutation K567A/R568A, and C-terminal truncated HAP1-DSRed construct /HAP1 amino acids 157-386 (FIGURE 6).
WB Human
Corradini E, Vallur R, Raaijmakers LM et al. Alterations in the cerebellar (phospho)proteome of a cGMP-dependent protein kinase knockout mouse. Mol. Cell Proteomics. 2014 Jun 12 [PMID: 24925903] (WB, Mouse)

Details:
6 week-old female WT and cGKI-KO mice (carrying a null mutation of the prkg1 gene, also termed cGKIL-/L- mice ) on a 129/Sv background were sacrificed and cerebella were kept frozen until testing. The tissues were lysed by sonication in lysis buffer and processed for WB using mouse anti-Huntingtin associated protein 1 (HAP1, Novus biological, 1:5000). HAP1 showed an increased expression in the cerebellum of cGKI-KO mice.
WB Mouse
Tang TS, Tu H, Chan EY, Maximov A, Wang Z, Wellington CL, Hayden MR, Bezprozvanny I. Huntingtin and huntingtin-associated protein 1 influence neuronal calcium signaling mediated by inositol-(1,4,5) triphosphate receptor type 1. Neuron;39(2):227-39. 2003 Jul 17. [PMID: 12873381] (IP, Mouse, Rat) IP Mouse, Rat
Torre ER, Coleman S, Yi H, Gutekunst CA. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods;125(1-2):27-32. 2003 May 30. [PMID: 12763227] (WB, Rat) WB Rat
Gutekunst, C et al. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. J Histochem Cytochem;51(6):841-52. 2003 Jun. [PMID: 12754295] (WB, ICC/IF, Mouse, Rat) WB, ICC/IF Mouse, Rat
Chan, E et al. Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Hum Mol Genet;11(8):945-59. 2002 Apr 15. [PMID: 11971876] (WB, Mouse) WB Mouse

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HAP1 Antibody (NB110-74569) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-74569AF405
Alexa Fluor 488 Labeled NB110-74569AF488
Alexa Fluor 647 Labeled NB110-74569AF647
Alexa Fluor 700 Labeled NB110-74569AF700
Biotin Labeled NB110-74569B
DyLight 405 Labeled NB110-74569V
DyLight 488 Labeled NB110-74569G
DyLight 550 Labeled NB110-74569R
DyLight 650 Labeled NB110-74569C
FITC Labeled NB110-74569F
HRP Labeled NB110-74569H

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HAP1 NB110-74569

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Bioinformatics

Gene Symbol HAP1