Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.
Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569] - Staining of rat hypothalamus.
Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more
Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Detection of HAP1 in mouse brain lysate.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 50% glycerol
0.05% Sodium Azide
Protein G purified
Western Blot 0.5-1.0 ug/ml
Flow Cytometry 1 ug per million cells
Immunohistochemistry 0.5-1.0 ug/ml
Immunohistochemistry-Frozen 0.5-1.0 ug/ml
Immunohistochemistry-Paraffin 0.5-1.0 ug/ml
This HAP1 antibody is useful for Immunohistochemistry frozen and paraffin embedded sections, Western blot, Flow Cytometry and Immunoprecipitation. In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Read Publications using NB110-74569 in the following applications:
HAP1 (Huntingtin-associated protein 1) is a cytoplasmic protein that interacts with several proteins such as Huntingtin, p150Glued, KLC, TrkA, TATA-binding protein, proBDNF etc. and plays a key role in microtubule-dependent transport, vesicular trafficking, nerve growth, transcriptional regulation and signal transduction. HAP1 was originally discovered as huntingtin interacting neuronal protein (htt), the Huntington's disease protein, however, unlike htt (expressed ubiquitously throughout body), HAP1 is enriched in brain with abundant expression in the hypothalamus, amygdala, brain stem etc. HAP1 has been associated with microtubule-dependent transporters dynactin p150 and kinesin light chain, as well as with transport of various vesicular proteins or membrane receptors, including BDNF-containing vesicles, EGFR, type 1 inositol (1,4,5)-triphosphate receptor, gamma -aminobutyric acid, type A, receptor, and TRKA. Hap1 is also implicated in microtubule-dependent trafficking in neurons, vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.