HAP1 Antibody (1B6)

Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.

Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569] - Staining of rat hypothalamus.

Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Detection of HAP1 in mouse brain lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: 1B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

HAP1 Antibody (1B6) Summary

• Immunogen: Fusion protein from rat HAP1. [UniProt# P54256]
• Specificity: This is specific for isoforms A and B.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1.0 ug/ml
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 0.5-1.0 ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0 ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
• Application Notes: This HAP1 antibody is useful for Immunohistochemistry frozen and paraffin embedded sections, Western blot, Flow Cytometry and Immunoprecipitation. In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
• Positive Control:

  Brain Lysate (NB820-59705)

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HAP1 Antibody (1B6)

• HAP2huntingtin-associated protein 2
• hHLP1
• HIP5
• HLP1
• HLPHAP-1
• huntingtin-associated protein 1
• Neuroan 1

Background

HAP1 (Huntingtin-associated protein 1) is a cytoplasmic protein that interacts with several proteins such as Huntingtin, p150Glued, KLC, TrkA, TATA-binding protein, proBDNF etc. and plays a key role in microtubule-dependent transport, vesicular trafficking, nerve growth, transcriptional regulation and signal transduction. HAP1 was originally discovered as huntingtin interacting neuronal protein (htt), the Huntington's disease protein, however, unlike htt (expressed ubiquitously throughout body), HAP1 is enriched in brain with abundant expression in the hypothalamus, amygdala, brain stem etc. HAP1 has been associated with microtubule-dependent transporters dynactin p150 and kinesin light chain, as well as with transport of various vesicular proteins or membrane receptors, including BDNF-containing vesicles, EGFR, type 1 inositol (1,4,5)-triphosphate receptor, gamma -aminobutyric acid, type A, receptor, and TRKA. Hap1 is also implicated in microtubule-dependent trafficking in neurons, vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAP1 Antibody (NB110-74569)(7)

Publications using NB110-74569

• Xiang J, Yang S, Xin N et al. DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome Proc. Natl. Acad. Sci. U.S.A Jan 30 2017 [PMID: 28137862] (IP, Mouse)
• Corradini E, Burgers PP, Plank M et al. Huntingtin associated protein (HAP1) is a cGMP-dependent kinase anchoring protein (GKAP), specific for the cGMP-dependent protein kinase Ibeta isoform J. Biol. Chem. 2015 Feb 04 [PMID: 25653285] (WB, Human)
  
  Details:
  HAP1 antibody used for WB analysis of totall cell lysates as well as the pulldowns performed in COS-7 cells which were co-transfected with HAP1 alone or with either PKG I alpha or PKG I beta (FIGURE 2). WB also performed on lysates and proteins pulled down in COS-7 cells co-expressing PKG I beta-GFP plasmid with HAP1-DSRed construct (Control), HAP1-DSRed bearing the double mutation K567A/R568A, and C-terminal truncated HAP1-DSRed construct /HAP1 amino acids 157-386 (FIGURE 6).
• Corradini E, Vallur R, Raaijmakers LM et al. Alterations in the cerebellar (phospho)proteome of a cGMP-dependent protein kinase knockout mouse. Mol. Cell Proteomics. 2014 Jun 12 [PMID: 24925903] (WB, Mouse)
  
  Details:
  6 week-old female WT and cGKI-KO mice (carrying a null mutation of the prkg1 gene, also termed cGKIL-/L- mice ) on a 129/Sv background were sacrificed and cerebella were kept frozen until testing. The tissues were lysed by sonication in lysis buffer and processed for WB using mouse anti-Huntingtin associated protein 1 (HAP1, Novus biological, 1:5000). HAP1 showed an increased expression in the cerebellum of cGKI-KO mice.
• Tang TS, Tu H, Chan EY, Maximov A, Wang Z, Wellington CL, Hayden MR, Bezprozvanny I. Huntingtin and huntingtin-associated protein 1 influence neuronal calcium signaling mediated by inositol-(1,4,5) triphosphate receptor type 1. Neuron;39(2):227-39. 2003 Jul 17. [PMID: 12873381] (IP, Mouse, Rat)
• Torre ER, Coleman S, Yi H, Gutekunst CA. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods;125(1-2):27-32. 2003 May 30. [PMID: 12763227] (WB, Rat)
• Gutekunst, C et al. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. J Histochem Cytochem;51(6):841-52. 2003 Jun. [PMID: 12754295] (WB, ICC/IF, Mouse, Rat)
• Chan, E et al. Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Hum Mol Genet;11(8):945-59. 2002 Apr 15. [PMID: 11971876] (WB, Mouse)

Bioinformatics

• Gene Symbol: HAP1