Senataxin Antibody

Images

 
Western Blot: Senataxin Antibody [NBP1-94712] - Input: HeLa whole cell lysate. Beads without antibody IP control. IP: IP from HeLa lysate.
Immunocytochemistry/ Immunofluorescence: Senataxin Antibody [NBP1-94712] - Senataxin immunofluorescence in fibroblasts. Image from verified customer review.
Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (transverse section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very ...read more
Flow Cytometry: Senataxin Antibody [NBP1-94712] - An intracellular stain was performed on Jurkat cells and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells ...read more
Immunocytochemistry/ Immunofluorescence: Senataxin Antibody [NBP1-94712] - Senataxin antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin ...read more
Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (verticle section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Senataxin Antibody Summary

Immunogen
A genomic peptide made to an internal portion of the human Senataxin protein (between residues 600-750) [UniProt Q7Z333]
Localization
Nucleus, nucleoplasm, nucleolus. Cytoplasm.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SETX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Immunoprecipitation 1:500
Application Notes
In ICC/IF, nuclear staining was observed in HeLa cells. In IP and Western blot a band is seen at ~300 kDa.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-94712 in the following applications:

Publications
Read Publications using
NBP1-94712 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28245518).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Senataxin Antibody

  • ALS4recessive, non-Friedreich type 1
  • AOA2FLJ43459
  • DKFZp781B151
  • KIAA0625FLJ12840
  • senataxin
  • SETX

Background

Senataxin (SETX, ALS4, AOA2, SCAR1) belongs to the DNA/NAM7 helicase family. The protein encoded for by Senataxin has a DNA/RNA helicase domain at its C-terminal end. Senataxin may play a role in RNA maturation. It may also be involved in DNA double-strand breaks damage response caused by oxidative stress. Cellular localization of Senataxin is limited to the nucleus and cytoplasm. Senataxin is abundantly expressed in skeletal muscle. Expression of Senataxin is also seen in heart, fibroblast, placenta, liver, and weakly in brain and lung. Post translational modifications of Senataxin include acetylation and phosphorylation. There are 3 known isoforms (302.9, 299.4 and 306.3 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Senataxin Antibody (NBP1-94712)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Human
(1)
Mouse
(1)
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Review for Senataxin Antibody (NBP1-94712) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-94712:
Filter by Applications
IF
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 05/24/2017
View

Summary

ApplicationImmunofluorescence
Sample TestedFibroblasts
SpeciesHuman
LotA-1

Blocking

Blocking Details1H room temperature, 5% Normal Goat Serum/0.3% Triton X-100 in PBS

Primary Anitbody

Dilution Ratio1:100 in 1% BSA

Secondary Antibody

Secondary Descriptiongoat anti rabbit alexafluor 555 conjugated
Secondary Manufacturer Cat#cell signaling 4413
Secondary Concentration1:1000

Details

Fixation DetailsFixed in PFA, Blocked in Normal Goat serum containing Triton X-100, Incubated overnight 1:100 in 1% BSA
Wash DescriptionPBS, 5 min each, 4 washes

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Senataxin Antibody (NBP1-94712). (Showing 1 - 1 of 1 FAQs).

  1. The customer is wondering when this antibody was first developed and if you have any papers which supports these images of WB and ICC and any other applications that it has been tested with?
    • NBP1-94712 has been tested and validated for use in Western Blot, Immunoprecipitation and ICC/IF in Human. Our 100% guarantee covers all of our products for the listed application and species. At this time there are no publications associated with this product. The images you see on the datasheet were obtained from our in house QC testing.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-94712AF350
Alexa Fluor 405 NBP1-94712AF405
Alexa Fluor 488 NBP1-94712AF488
Alexa Fluor 532 NBP1-94712AF532
Alexa Fluor 594 NBP1-94712AF594
Alexa Fluor 647 NBP1-94712AF647
Alexa Fluor 700 NBP1-94712AF700
Alexa Fluor 750 NBP1-94712AF750
Allophycocyanin/Cy7 NBP1-94712APCCY7
Biotin NBP1-94712B
DyLight 350 NBP1-94712UV
DyLight 405 NBP1-94712V
DyLight 488 NBP1-94712G
DyLight 550 NBP1-94712R
DyLight 650 NBP1-94712C
DyLight 680 NBP1-94712FR
DyLight 755 NBP1-94712IR
FITC NBP1-94712F
HRP NBP1-94712H
Janelia Fluor 549 NBP1-94712JF549
Janelia Fluor 646 NBP1-94712JF646
PE/Atto594 NBP1-94712PEATT594
PE/Cy5.5 NBP1-94712PECY55
PE/Cy7 NBP1-94712PECY7

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Recent Reviews

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Anonymous
05/24/2017
Application: IF
Species: Human

Bioinformatics

Gene Symbol SETX
Entrez
Uniprot