Senataxin Antibody - BSA Free

Western Blot: Senataxin Antibody [NBP1-94712] - Input: HeLa whole cell lysate. Beads without antibody IP control. IP: IP from HeLa lysate.

Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Senataxin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (transverse section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very strong staining in the cytoplasm and the nuclei of the muscle cells. No signal was found in the perimysium and endomycium area (connective tissue) of the section.

Flow Cytometry: Senataxin Antibody [NBP1-94712] - An intracellular stain was performed on Jurkat cells and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .

Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - Senataxin antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).

Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - Senataxin immunofluorescence in fibroblasts. Image from verified customer review.

Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (verticle section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very strong staining in the cytoplasm and the nuclei of the muscle cells. No signal was found in the perimysium and endomycium (connective tissue) of the section.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Senataxin Antibody - BSA Free Summary

• Immunogen: A genomic peptide made to an internal portion of the human Senataxin protein (between residues 600-750) [UniProt Q7Z333]
• Localization: Nucleus, nucleoplasm, nucleolus. Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SETX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Immunoprecipitation 1:500
  • Western Blot 1:500
• Application Notes: In ICC/IF, nuclear staining was observed in HeLa cells. In IP and Western blot a band is seen at ~300 kDa.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28245518).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Senataxin Antibody - BSA Free

• ALS4recessive, non-Friedreich type 1
• AOA2FLJ43459
• DKFZp781B151
• KIAA0625FLJ12840
• senataxin
• SETX

Background

Senataxin (SETX, ALS4, AOA2, SCAR1) belongs to the DNA/NAM7 helicase family. The protein encoded for by Senataxin has a DNA/RNA helicase domain at its C-terminal end. Senataxin may play a role in RNA maturation. It may also be involved in DNA double-strand breaks damage response caused by oxidative stress. Cellular localization of Senataxin is limited to the nucleus and cytoplasm. Senataxin is abundantly expressed in skeletal muscle. Expression of Senataxin is also seen in heart, fibroblast, placenta, liver, and weakly in brain and lung. Post translational modifications of Senataxin include acetylation and phosphorylation. There are 3 known isoforms (302.9, 299.4 and 306.3 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Senataxin Antibody (NBP1-94712)(6)

Publications using NBP1-94712

• Gatti, V;Fierro, C;Compagnone, M;La Banca, V;Mauriello, A;Montanaro, M;Scalera, S;De Nicola, F;Candi, E;Ricci, F;Fania, L;Melino, G;Peschiaroli, A; delta Np63-Senataxin circuit controls keratinocyte differentiation by promoting the transcriptional termination of epidermal genes Proceedings of the National Academy of Sciences of the United States of America [PMID: 35235452]
• Liu Z, Gao X, Zhou Z Et Al. San1 deficiency leads to cardiomyopathy due to excessive R-loop-associated DNA damage and cardiomyocyte hypoplasia Biochimica et biophysica acta. Molecular basis of disease 2021-07-30 [PMID: 34339838] (WB)
• Richard P, Feng S, Tsai YL et al. SETX (senataxin), the helicase mutated in AOA2 and ALS4, functions in autophagy regulation Autophagy 2020-07-18 [PMID: 32686621]
• Choudhury SD, Vs A, Mushtaq Z, Kumar V. Altered translational repression of an RNA-binding protein, Elav by AOA2-causative Senataxin mutation. Synapse. 2017-05-01 [PMID: 28245518] (ICC/IF, Mouse)
• Richard P, Feng S, Manley JL. A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage. Genes Dev. 2013-10-15 [PMID: 24105744] (WB, Human)
• Feng S Nucleolar transcription and its connections to nucleolar homeostasis and mitochondrial stress responses Thesis 2022-01-01

Review for Senataxin Antibody (NBP1-94712) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Jennifer Fox IF Human 05/24/2017

Summary

• Application: Immunofluorescence
• Sample Tested: fibroblasts
• Species: Human
• Lot: A-1

FAQs for Senataxin Antibody (NBP1-94712). (Showing 1 - 1 of 1 FAQs).

1. The customer is wondering when this antibody was first developed and if you have any papers which supports these images of WB and ICC and any other applications that it has been tested with?
   • NBP1-94712 has been tested and validated for use in Western Blot, Immunoprecipitation and ICC/IF in Human. Our 100% guarantee covers all of our products for the listed application and species. At this time there are no publications associated with this product. The images you see on the datasheet were obtained from our in house QC testing.

Bioinformatics

• Gene Symbol: SETX
• Entrez:
  • 23064(Human)
• Uniprot:
  • Q7Z333(Human)