Desmoglein-3 Antibody (5H10)

Images

 
Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 expression in HaCat cell lysate using NBP1-78984.
Immunocytochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984] - The cultured primary mouse skin keratinocytes were stained at 1:150. The image shows the cytosolic staining. This image was submitted via customer Review.
Immunohistochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 on human skin cancer using NBP1-78984.
Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Desmoglein 3 (5H10) antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Desmoglein 3 (5H10) antibody was tested in oral HNSCC cells fixed with 1% formaldehyde permeabilized in 0.1% triton x-100.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, ICC/IF
Clone
5H10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Desmoglein-3 Antibody (5H10) Summary

Immunogen
The extracellular domain of human Desmoglein 3 [Swiss-Prot# P32926]
Localization
Cell membrane; Single-pass type I membrane protein. Cell junction > desmosome
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DSG3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Immunocytochemistry 1:150
Application Notes
This Desmoglein 3 (5H10) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western blot, where a band can be seen at approximately 130 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-78984 in the following applications:

Publications
Read Publications using
NBP1-78984 in the following applications:

  • DB
    1 publication
  • 3 publications
  • IP
    3 publications
  • WB
    6 publications

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Desmoglein-3 Antibody (5H10)

  • Cadherin family member 6
  • CDHF6
  • CDHF6130-kD pemphigus vulgaris antigen
  • desmoglein 3 (pemphigus vulgaris antigen)
  • desmoglein 3
  • Desmoglein3
  • Desmoglein-3
  • DKFZp686P23184,130 kDa pemphigus vulgaris antigen
  • DSG3
  • pemphigus vulgaris antigen
  • PVA

Background

Desmoglein 3 (Dsg3) is a member of sub-family the desmoglein (DSGs) and it mainly serves as adhesion component of intercellular desmosome junctions. DSGs serve essential part of the core complex of desmosomes, and together with desmocollin (DSCs), they represents transmembrane proteins which mediate adhesion by transinteracting with cadherins of the opposing cell. Homophilic (e.g., DSG3-DSG3) as well as heterophilic (e.g., DSC3-DSG1) transinteraction have been reported for these proteins and within the desmosome, DSGs/DSCs are anchored to the intracellular plaque proteins plakoglobin, plakophilins, and desmoplakin, the latter of which mediates connection to the intermediate filament cytoskeleton. Desmoglein 3 is predominately expressed in stratified squamous epithelia including epidermis, tongue, tonsil, esophagus and carcinomas. Desmoglein 3 has been characterized as an auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease, wherein the binding of anti-desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Desmoglein-3 Antibody (NBP1-78984)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: DB, ICC/IF, IP, WB.


Filter By Application
DB
(1)
ICC/IF
(3)
IP
(3)
WB
(6)
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Human
(6)
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Showing Publications 1 - 7 of 7.
Publications using NBP1-78984 Applications Species
Walter E, Vielmuth F, Rotkopf L et al. Different signaling patterns contribute to loss of keratinocyte cohesion dependent on autoantibody profile in pemphigus. Sci Rep. 2017 Jun 15 [PMID: 28620161] (WB, Human) WB Human
Tsang SM, Liu L, Teh MT, Wheeler A, Grose R, Hart IR, Garrod DR, Fortune F, Wan H. Desmoglein 3, via an interaction with E-cadherin, is associated with activation of Src. PLoS One. 5(12):e14211. 2010 Dec 3. [PMID: 21151980] (WB, ICC/IF, Human) WB, ICC/IF Human
Mahoney MG, Hu Y, Brennan D, Bazzi H, Christiano AM, Wahl JK 3rd. Delineation of diversified desmoglein distribution in stratified squamous epithelia: implications in diseases. Exp Dermatol. 15(2):101-9. 2006 Feb. [PMID: 16433681] (IP, WB, Human) IP, WB Human
Lucchese A, Mittelman A, Lin MS, Kanduc D, Sinha AA. Epitope definition by proteomic similarity analysis: identification of the linear determinant of the anti-Dsg3 MAb 5H10. J Transl Med. 2(1):43. 2004 Dec 11. [PMID: 15588331] (DB, Human) DB Human
Wahl JK 3rd. Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics. 21(1):37-44. 2002 Feb. [PMID: 11991815] (WB, IP, ICC/IF) WB, IP, ICC/IF
Weiske J, Schoneberg T, Schroder W, Hatzfeld M, Tauber R, Huber O. The fate of desmosomal proteins in apoptotic cells. J Biol Chem. 276(44):41175-81. 2001 Nov 2. [PMID: 11500511] (WB, IP, ICC/IF, Human) WB, IP, ICC/IF Human
Proby CM, Ota T, Suzuki H, Koyasu S, Gamou S, Shimizu N, Wahl JK, Wheelock MJ, Nishikawa T, Amagai M. Development of chimeric molecules for recognition and targeting of antigen-specific B cells in pemphigus vulgaris. Br J Dermatol. 142(2):321-30. 2000 Feb. [PMID: 10730768] (WB, Human) WB Human

Review for Desmoglein-3 Antibody (NBP1-78984) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-78984:
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ICC
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Desmoglein-3 NBP1-78984
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reviewed by:
Qi Miao
ICC Mouse 06/22/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedPrimary skin keratinocytes
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Desmoglein-3 Antibody (NBP1-78984) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-78984AF405
Alexa Fluor 488 Labeled NBP1-78984AF488
Alexa Fluor 647 Labeled NBP1-78984AF647
Alexa Fluor 700 Labeled NBP1-78984AF700
Biotin Labeled NBP1-78984B
DyLight 350 Labeled NBP1-78984UV
DyLight 405 Labeled NBP1-78984V
DyLight 488 Labeled NBP1-78984G
DyLight 550 Labeled NBP1-78984R
DyLight 650 Labeled NBP1-78984C
DyLight 680 Labeled NBP1-78984FR
DyLight 755 Labeled NBP1-78984IR
FITC Labeled NBP1-78984F
HRP Labeled NBP1-78984H

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Blogs on Desmoglein-3.

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Recent Reviews

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Qi Miao
06/22/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol DSG3
Entrez
Uniprot