Novus Biologicals releases new PCNA Antibody

Novus Biologicals launched a new mouse monoclonal anti-PCNA antibody this week (cat# NB500-106).  Proliferating Cell Nuclear Antigen (PCNA), also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways.  It binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).  PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis).

Novus' new PCNA antibody (clone PC10) was tested in Western blot and Immunohistochemistry.  By Western blot analysis, the antibody was tested on human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE) cell extracts producing clear bands  at 30kDa. The immunohistochemical analysis of this antibody on paraffin-embedded human colon carcinoma showed nuclear localization of PCNA.

This PCNA antibody has been mentioned in two scientific publications:

  • Waseem NH and Lane DP. Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.  J Cell Sci. 1990 May;96 (Pt 1):121-9. [PMID: 1695635]
  • Woods AL, et al. The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis. Histopathology. 2002 Sep;41(3A):165-71. [PMID: 12405950]




For more information on this new PCNA antibody or to inquire about product collaborations, please contact the Novus Product Development Team by calling 303-730-1950 or via e-mail at

Release Date: 
Tuesday, March 30, 2010 - 06:00