Lipid-and-Metabolism reagents: Novus Biologicals

NBP1-04676

PGC1 alpha Antibody - BSA Free

(11 Reviews)

(303 Publications)

0.1 ml

NBP1-04676

Mitochondrial content and biogenesis. Immunoblot analysis of PGC1 alpha in MEFs (n = 7; t = 5.764; df = 6). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-12662-3), licensed under a CC-BY license.

HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-PGC1 alpha Antibody NBP1-04676 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Analysis of a FFPE section of mouse prostate using rabbit polyclonal PGC1 alpha Antibody at 1:200 dilution. The antibody generated an expected strong nuclear (punctate in some cells) and a weak cytoplasmic staining in the glandular cells lining of tubule-alveolar gland.

An intracellular stain was performed on A431 cells with PGC1 alpha Antibody NBP1-04676 (blue) and matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

<b>Biological Strategies Validation. </b>Vehicle or RU 486 (5 mg/kg) injected mice were presented with/without corticosterone (10 mg/kg) for 3 days. The expressions of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha NBP1-04676) and NIX (BNIP3L NBP1-88558) were visualized via western blotting. Loading control is beta-actin. n = 5. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105/) licensed under a CC-BY license.

Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL PGC1 alpha Antibody (Molecular weight: 91 KDa) in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence

Western Blot of total protein from murine skeletal muscle tissue using PGC1 alpha Antibody. Image from verified customer review.

HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with PGC1 alpha Antibody [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Analysis of PGC1 alpha Antibody in mouse prostate using DAB with hematoxylin counterstain.

An intracellular stain was performed on HeLa cells with NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

An intracellular stain was performed on HepG2 cells with PGC1 alpha Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of A431 human squamous carcinoma parental cell line and PGC1 alpha knockout (KO) A431 cell line. PVDF membrane was probed with 1:1000 of Rabbit Polyclonal PGC1 alpha Antibody (Catalog # NBP1-04676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PGC1 alpha at approximately 105 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.

DM1-derived fibroblasts have no changes in mitochondria biogenesis. (A, B) Representative images of immunofluorescence of TOMM20, and PGC1-alpha in DM1 and control fibroblasts (n=3). (C) Medium fluorescence intensity of MitoTracker Red FM in control (n=3) and DM1 cells (n=5) and (D) of Rhodamine 123 in DM1 and control fibroblasts (n=3). (E) mRNA levels of TFAM transcription factor (n=3). (F) mRNA levels of OPA1, MFN1, MFN2, DRP1 and PARKIN in DM1 and control fibroblasts (n≥2).

A431 human skin carcinoma cell line was stained with Rabbit anti-PGC1 alpha Affinity-purified Polyclonal Antibody conjugated to Phycoerythrin (Catalog # NBP1-04676PE, blue histogram) or matched control antibody (Catalog # NBP2-24983, orange histogram).

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Effect of butein T on SC adipose tissue browning in the ThermoMouse. SC fat was obtained from ThermoMouse exposed for 4 days to butein (10 or 20 mg/kg; n = 4) or vehicle (n = 2). A BAT lysate of a ThermoMouse was used as a positive control for UCP-1 protein levels. Tissues were homogenized & protein lysates (50 µg for SC fat & 10 µg for BAT) were used in western blotting for PGC1-alpha (a) & UCP-1 (c). Panels (b) & (d) illustrate the densitometric quantitation of the PGC1-alpha & UCP-1 western blot, respectively, normalized for the levels of the loading control alpha -tubulin. Abbreviations: subcutaneous (SC); brown adipose tissue (BAT); peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1-alpha ) & uncoupling protein-1 (UCP-1). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31094273), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Expression of the mitochondrial biogenesis proteins: SIRT1, PGC1 alpha , TFAM & SIRT3 in H1299, H1299r, P31 & P31r cell lysates as determined by immunoblot analysisWhole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 & P31r cells. Proteins (90 μg) were resolved in 10% SDS-PAGE gels & transferred to a PVDF membrane. Blots were probed for SIRT1, PGC1 alpha , TFAM & SIRT3 or the loading control gamma -tubulin. Figure shows representative blots of (A) of SIRT1, (B) PGC1 alpha , (C) SIRT3, (D) TFAM protein expression; each from three independent experiments. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Maternal exercise during pregnancy on mitochondrial biogenesis in the fetal hearts. (A) Levels of relative mRNA expression measured by qRT‐PCR. n = 9–12/group. Maternal exercise during pregnancy did not alter levels of mRNA in Ppargc1a & Tfam, while it significantly upregulated the levels of mRNA in Nrf1 & Nrf2. (B–D) Densitometric analyses of protein expression levels relative to the sedentary group with representative images of western blots were shown. No significant differences in PGC‐1 alpha , NRF1, & NRF2 (P > 0.05). n = 5–6/group. * P < 0.05, significantly different from the sedentary group. Black bar: fetal hearts from sedentary dams; gray bar: fetal hearts from exercised dams. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28292876), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody - BSA Free [NBP1-04676] - DM1-derived fibroblasts have no changes in mitochondria biogenesis. (A, B) Representative images of immunofluorescence of TOMM20, & PGC1-alpha in DM1 & control fibroblasts (n=3). (C) Medium fluorescence intensity of MitoTracker Red FM in control (n=3) & DM1 cells (n=5) & (D) of Rhodamine 123 in DM1 & control fibroblasts (n=3). (E) mRNA levels of TFAM transcription factor (n=3). (F) mRNA levels of OPA1, MFN1, MFN2, DRP1 & PARKIN in DM1 & control fibroblasts (n≥2). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32310829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Effect of BGP-15 treatment on the regulation of mitochondrial biogenesis in NRCM cells. Western blot analysis of PGC-1-alpha , CREB, & VDAC proteins as well as densitometric evaluation is shown. GAPDH was used as a loading control. Control group: cells without any treatment; BGP-15 group: cells with only 50 μM BGP-15 for 0.5 hours; H2O2 group: cells with 150 μM H2O2 for 0.5 hours; H2O2+BGP-15 group: cells with 150 μM H2O2 & 50 μM BGP-15 for 0.5 hours. Values are mean ± SEM (n = 4). ∗p < 0.05 vs. Control, ∗∗p < 0.01 vs. Control, §§p < 0.01 vs H2O2 group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33728024), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Feeding the diabetes mellitus (DM) mice with the RSV‐enriched diet improved mitochondrial biogenesis in the quadriceps muscle. A) Representative western blots using antibodies against nuclear respiratory factor (NRF‐1), ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), cytochrome c oxidase subunit IV (Cox IV), peroxisome proliferator‐activated receptor‐ gamma coactivator 1‐ alpha (PGC‐1 alpha ), & GAPDH. After quantification, the B) NRF‐1/GAPDH, C) ATP5B/GAPDH, D) Cox IV/GAPDH, & E) PGC‐1 alpha /GAPDH ratios were calculated in mice fed either the control (CTL) or RSV‐enriched diet. GAPDH was used as a loading control. F) Representative western blots using antibodies against mitochondrial transcription factor A (mtTFA) & voltage‐dependent anion channel (VDAC), & the mtTFA/VDAC ratio was determined. The mitochondrial protein VDAC was used as a loading control. Data are expressed as fold change versus CTL & reported as mean ± SD, n = 4–6 per group; *p < 0.05; **p < 0.01 between CTL & DM groups. #p < 0.05 between DM & DM+RSV groups; ††p < 0.05 between CTL+RSV & DM+RSV groups. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29578301), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Effects of SRTAW04 on expression of markers of mitochondrial & anti-oxidant function. (a) Western blot of protein extracts from optic nerve & retina of control (lanes 1–4), MHV-A59 infected (lanes 5–8), & MHV-A59 infected + SRTAW04-treated (lanes 9–12) mice. Average levels of SDHb measured by Western blotting (n = 4/group) showed a significant (*p < 0.05) decrease in protein extracts from optic nerves (a,b) & retinas (a,d) of MHV-A59 infected mice 7 days post-inoculation, compared to control mice. MHV-A59 infected mice treated with SRTAW04 (100 mg/kg/day) showed a significant increase (@p < 0.05) of SDHb protein expression compared to untreated MHV-A59 infected mice. There is a significant decrease (*p < 0.05) in expression of SOD2 (n = 4/group) in optic nerves (a,c) & retinas (a,e) of MHV-A59 infected mice compared to control mice, & treatment with SRTAW04 significantly (@p < 0.05) attenuates that change. PGC1-alpha expression shows a significant (*p < 0.05) decrease in retinas (a,f) (n = 4/group) during MHV-A59 infection & treatment with SRTAW04 for 7 days significantly (@p < 0.05) increases the PGC1-alpha protein levels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24383546), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - RSV treatment increases markers of mitochondrial function in stressed RGC-5 cells. Staurosporine-differentiated RGC-5 cells were cultured with or without 500 μM H2O2, & with or without 0.25 μM RSV for 24 h. (A) Western blot analysis shows a significant decrease in SDH expression (**p < 0.01) during H2O2 treatment which is attenuated by treatment with RSV (*p < 0.05). (B) Western blot analysis shows similar effects on SOD2 expression. The significant decrease (**p < 0.01) during H2O2 treatment, compared to controls, is not found in cells treated with RSV (*p < 0.05). (C) Protein extracts were immunoprecipitated with anti-PGC-1 alpha antibodies, blotted, & hybridized with anti-PGC-1 alpha & anti-acetylated lysine antibodies to assess the acetylation state of PGC-1 alpha . H2O2 treatment significantly increases the proportion of acetylated PGC-1 alpha (*p < 0.05) compared to controls, & RSV & SRTAW04 treatment each prevent this acetylation (*p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23293585), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Pequi extract increases expression of antioxidant enzymes in HCAEC. Cells were cultured, pre-treated with pequi extract (25 μg/mL) for 24 h under normoxia conditions, & total protein content was extracted for Western blots analysis for Superoxide dismutase (SOD)-1 (A), SOD-2 (B), catalase (C), glutathione peroxidase—GPx (D), sirtuin 1—SIRT-1 (E), peroxisome-proliferator-activated receptor gamma coactivator 1-alpha (PCG1-alpha ) (F), glycogen synthase kinase 3 beta (GSK3 beta ) (G), & microtubule-associated protein 1A/1B-light chain 3 (LC3) (H). Western blots were normalized using alpha -tubulin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as loading control. Five independent Western blot experiments were carried for each condition. Representative images are shown here. Statistical analysis was performed using Student’s t-test (p < 0.05). OD: Optical Density. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326129), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - EMPA treatment increased mitochondrial biogenesis. (A) Left, Transmission electron microscopy showing the morphology of mitochondria in sham, sham + EMPA, TAC & TAC + EMPA groups & Right, quantitative analysis of mitochondrial size & counts. Results are expressed as mean ± SEM, n = 3–5, *p < 0.05 vs. corresponding sham, †p < 0.05 vs. corresponding TAC. (B) Left, Representative blots of mitochondrial biogenesis-related proteins & Right, quantitative results. (C) Relative mRNA levels of PGC1 alpha , NRF1, TFAM & COX1. Results are expressed as mean ± SEM, n = 5–7, *p < 0.05 vs. corresponding sham group, †p < 0.05 vs. corresponding TAC vehicle group. One-way ANOVA & Tukey post hoc test. EMPA, empagliflozin; SEM, standard error of the mean; TAC, transverse aortic constriction; PGC1-alpha , peroxisome proliferator-activated receptor gamma coactivator 1-alpha; NRF-1, nuclear respiratory factor 1; TFAM, mitochondrial transcription factor A; COX1, cyclooxygenase1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35647080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Mitochondrial content & PGC‐1 alpha levels are increased in cells with greater TFEB protein expression. (a) Mitochondrial content was measured in SH‐SY5Y cells & TFEB‐DDK cells under basal conditions by assaying citrate synthase (CS) activity. **p < 0.01 versus SH‐SY5Y; n = 6. (b) Western blotting for the mitochondrial proteins prohibitin 1 & cytochrome oxidase (COX) subunit IV also indicated that mitochondrial content was increased in TFEB‐DDK cells. (c) PGC‐1 alpha mRNA levels were measured in SH‐SY5Y cells & TFEB‐DDK cells under basal conditions by qPCR. Data normalised against beta ‐actin mRNA levels. **p < 0.01 versus SH‐SY5Y cells; n = 3. (d) SH‐SY5Y cells were treated with scrambled (scram) or TFEB siRNA for 72 h & PGC‐1 alpha mRNA levels measured. *p < 0.05 versus scram; n = 4. (e) PGC‐1 alpha protein levels were detected by western blotting in total cell lysates of SH‐SY5Y cells, TFEB‐DDK cells & a SH‐SY5Y cell line over‐expressing human PGC‐1 alpha . The fold increase in PGC‐1 alpha protein density for this blot & PGC‐1 alpha mRNA levels of the respective cell lines are reported underneath the blot. (f) TFEB‐DDK cells were treated with 10 μM carbonyl cyanide m‐chlorophenylhydrazone (CCCP) for 18 h & cytosolic & nuclear fractions prepared. PGC‐1 alpha protein was detected in cytosolic (3.5% of total volume) & nuclear fractions (35% of total volume) by western blotting. The purity of the nuclear & cytosolic fractions was assessed by western blotting using lamin A & beta ‐actin antibodies respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26509433), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - PGC1 alpha & HNF4 alpha interacts & binds to Vnn1 promoter in IUGR asthmatic mice. Asthma was induced with OVA in IUGR mice. PBS induction was used as the control. Nuclear protein was extracted from lung tissues. (A) Immunoblot assay was performed for expressions of PGC1 alpha & HNF4 alpha . (B) IP was performed using mouse anti-HNF4 alpha antibody & Protein G-coupled agarose beads, followed by immunoblot with rabbit-anti-PGC1 alpha antibody. The normal mouse IgG was used as the IP control. (C) In primary cultured bronchial epithelia cells isolated from IUGR mice injected with OVA or PBS, ChIP assay was performed using mouse anti-HNF4 alpha or rabbit anti-PGC1 alpha antibodies. The normal mouse or rabbit IgG was used as the control. Graphics show the percentage of total DNA immunoprecipitated by each indicated antibody. Data are shown as mean±s.d. n=4. *P<0.01 OVA versus PBS. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32139393), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

An intracellular stain was performed on A431 human skin carcinoma cell line using Rabbit anti-PGC1 alpha Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-04676AF647, blue histogram) or matched control antibody (Catalog # NBP2-24981AF647, orange histogram) at 2.5 µg/mL for 30 minutes at RT.

Reactivity:

Hu, Mu, Rt, Po, Gt, Ha, Sh, Sq, Ca, Eq, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Gt, Ha, Sh, Sq, Ca, Eq, Pm

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-04676AF350
Alexa Fluor 405	NBP1-04676AF405
Alexa Fluor 488	NBP1-04676AF488
Alexa Fluor 532	NBP1-04676AF532
Alexa Fluor 594	NBP1-04676AF594
Alexa Fluor 647	NBP1-04676AF647
Alexa Fluor 700	NBP1-04676AF700
Alexa Fluor 750	NBP1-04676AF750
Allophycocyanin	NBP1-04676APC
Biotin	NBP1-04676B
CoraFluor 1	NBP1-04676CL1
DyLight 350	NBP1-04676UV
DyLight 405	NBP1-04676V
DyLight 488	NBP1-04676G
DyLight 550	NBP1-04676R
DyLight 594	NBP1-04676DL594
DyLight 650	NBP1-04676C
DyLight 680	NBP1-04676FR
DyLight 755	NBP1-04676IR
FITC	NBP1-04676F
HRP	NBP1-04676H
Janelia Fluor 525	NBP1-04676JF525
Janelia Fluor 549	NBP1-04676JF549
Janelia Fluor 646	NBP1-04676JF646
Janelia Fluor 669	NBP1-04676JF669
PE	NBP1-04676PE
PerCP	NBP1-04676PCP
mFluor Violet 450 SE	NBP1-04676MFV450
mFluor Violet 500 SE	NBP1-04676MFV500
mFluor Violet 610 SE	NBP1-04676MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-04676SS

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NB100-2288

BMAL1 Antibody - BSA Free

(1 Review)

(85 Publications)

0.1 ml

NB100-2288

<b>Biological Strategies Validation. </b>Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Inhibition of mTOR signaling with Rapamycin rescues PTEN-driven accumulation of BMAL1A. Tissue samples and graphic show BMAL1 in K14cre PtenF/F mice and control (arrows). Note a significant amount of BMAL1 expression in PTEN mutant mice. K14cre PtenF/F mice and control littermates received Rapamycin or vehicle every other day for 15 days. Rapamycin treatment reduces the expression of nuclear BMAL1 in K14cre PtenF/F mice (arrowhead) (*** p0.05). Scale bars -30um. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5173143/) licensed under a CC-BY license.

Western Blot: BMAL1 Antibody [NB100-2288] - Oxidation causes accumulation of BMAL1. Western blot assay demonstrates time-dependent accumulation of BMAL1 upon oxidative stress in HNSCC cells (*p0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27285754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BMAL1 Antibody [NB100-2288] - Small interference RNA targeting Raptor & Rictor disrupts BMAL1 accumulation in HNSCCTargeted disruption of Raptor (A-B) & Rictor (C-D) using siRNA results in a dose-dependent downregulation of BMAL1 in HNSCC cells. E. Disruption of PTEN by protein oxidation causes activation of mTOR signaling, resulting in accumulation of BMAL1. Notably, inhibition of mTOR signaling, particularly mTORC1 & mTORC2, results in restoration of normal BMAL1 levels in the epidermis of mice & head & neck cancer cells. These results demonstrate a novel role for mTOR in regulating nuclear levels of the core clock gene BMAL1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27285754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BMAL1 Antibody [NB100-2288] - Targeted disruption of PTEN in vitro & in vivo induces activation of pS6 & BMAL1A. & B. Targeted inhibition of PTEN using siRNA results in concentration-dependent inhibition of PTEN protein in HNSCC cells. HNSCC cells show accumulation of BMAL1 & pS6 in response to PTEN inhibition. C. Immunofluorescence assay to detect BMAL1 in PTEN conditional knockout mice (K14cre PtenF/F) & control littermates. Immunofluorescences & graphic show high accumulation of nuclear BMAL1 in K14cre PtenF/F mice compared to control mice (K14cre). Scale bars represent 50 μm. Black & white images depict cells positive for BMAL1 in K14cre PtenF/F mice (arrows) compared to few BMAL-positive cells in control mice (arrowhead). Scale bars represent 10 μm. (**p<0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27285754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BMAL1 Antibody [NB100-2288] - Oxidation causes accumulation of BMAL1A. Head & neck cancer cells have different expression levels of the core clock protein BMAL1. B. Immunofluorescence assay depicts accumulation of ROS (green channel) & BMAL1 (red channel) upon oxidation. C. & D. Western blot assay demonstrates time-dependent accumulation of BMAL1 upon oxidative stress in HNSCC cells (*p<0.05, **p<0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27285754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: BMAL1 Antibody [NB100-2288] - Targeted disruption of PTEN in vitro & in vivo induces activation of pS6 & BMAL1A. & B. Targeted inhibition of PTEN using siRNA results in concentration-dependent inhibition of PTEN protein in HNSCC cells. HNSCC cells show accumulation of BMAL1 & pS6 in response to PTEN inhibition. C. Immunofluorescence assay to detect BMAL1 in PTEN conditional knockout mice (K14cre PtenF/F) & control littermates. Immunofluorescences & graphic show high accumulation of nuclear BMAL1 in K14cre PtenF/F mice compared to control mice (K14cre). Scale bars represent 50 μm. Black & white images depict cells positive for BMAL1 in K14cre PtenF/F mice (arrows) compared to few BMAL-positive cells in control mice (arrowhead). Scale bars represent 10 μm. (**p<0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27285754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Astrocyte Bmal1 regulates genes with conflicting effects on A beta deposition. (A) Topro, GFAP, & BMAL1 staining in CA1 hippocampus of 4-month-old BMAL1 aKO; APP/PS1-21 mice & Cre- controls (scale bar = 100 µm). Arrows indicate astrocyte nuclei quantified as indicated by Topro nuclei surrounded by GFAP positivity. Blue circles indicate nuclei quantified as BMAL1 negative. Quantification of astrocytes counted as BMAL1- or BMAL1+ is shown on the right. n = 5 mice per group, **** = p < 0.0001 by two-way ANOVA with Sidak multiple comparisons test. (B) Heatmap of Fluidigm qPCR analysis of 20 genes involved in the circadian clock, glial activation, & Alzheimer’s Disease in cortex from Aldh1l1-CreERT2; Bmal1fl/fl mice & Cre- controls with or without APP/PS1-21 or APPNL-G-F/wt (n = 6–8 mice per group). Two-way ANOVA analysis: c = significant main effect of Cre genotype, m = main effect of A beta model, c*m = interaction effect of cre & A beta model, - = no significance (all p < 0.05). (C) Individually plotted genes from A. * = p < 0.05, ** = p < 0.005, *** = p < 0.0005 by two-way ANOVA with Sidak multiple comparisons test. Panel B was made using GraphPad Prism version 9.2 (//www.graphpad.com). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35110643), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Am, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Am, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2288AF350
Alexa Fluor 405	NB100-2288AF405
Alexa Fluor 488	NB100-2288AF488
Alexa Fluor 532	NB100-2288AF532
Alexa Fluor 594	NB100-2288AF594
Alexa Fluor 647	NB100-2288AF647
Alexa Fluor 700	NB100-2288AF700
Alexa Fluor 750	NB100-2288AF750
Allophycocyanin	NB100-2288APC
Biotin	NB100-2288B
CoraFluor 1	NB100-2288CL1
DyLight 350	NB100-2288UV
DyLight 405	NB100-2288V
DyLight 488	NB100-2288G
DyLight 550	NB100-2288R
DyLight 594	NB100-2288DL594
DyLight 650	NB100-2288C
DyLight 680	NB100-2288FR
DyLight 755	NB100-2288IR
FITC	NB100-2288F
HRP	NB100-2288H
Janelia Fluor 525	NB100-2288JF525
Janelia Fluor 549	NB100-2288JF549
Janelia Fluor 646	NB100-2288JF646
Janelia Fluor 669	NB100-2288JF669
PE	NB100-2288PE
PerCP	NB100-2288PCP
mFluor Violet 450 SE	NB100-2288MFV450
mFluor Violet 500 SE	NB100-2288MFV500
mFluor Violet 610 SE	NB100-2288MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2288SS

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NB600-582

SREBP1 Antibody (2A4) - BSA Free

(73 Publications)

0.1 ml

NB600-582

Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

<b>Genetic Strategies Validation. </b>Western Blot: SREBP1 Antibody (2A4) [NB600-582] - KO and OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, and WT Bel-7402 cells. Data were expressed as mean -/+ standard error of the mean. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. WT group, respectively.

Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Effect of GEN on the expression of Nrf2‐related signalling proteins in OA (660 μmol/L) & PA (330 μmol/L)‐induced HepG2 cells. GEN was added into HepG2 cells prior 1 h to stimulation of OA & PA for 18 h. A‐C, Protein expression of Nrf2, PPAR alpha , PPAR gamma in nucleus & HO‐1 in cytoplasm was detected by Western blot. (D & E) Protein expression of P‐ACC, ACC, P‐AKT, AKT, P‐AMPK alpha , AMPK alpha , P‐AMPK beta & AMPK beta was detected by Western blot. F‐H, Protein expression of PI3K, P‐mTORC, mTORC, P‐S6K, S6K, P‐S6, S6, SREBP‐1c & HMGB1 was detected by Western blot. The similar results were collected from three dependent experiments. All data were expressed by mean ± SEM (n = 5 in each group). ##P < .01 vs Control Group; *P < .05 & **P < .01 vs OA & PA Group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32293113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - KO & OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. (A) Total cellular TG content of SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. (B) HPLC-MS determination of the SFAs to MUFAs ratios (palmitic acid to palmitoleic acid, 16:0 to 16:1) (stearic acid to oleic acid, 18:0 to 18:1) as well as the content of 16:0, 16:1, 18:0, & 18:1 in SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. (C) The transcript level of lipid-associated genes in SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. (D & E) The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. Data were expressed as mean ± standard error of the mean. *P < 0.05, **P < 0.01, & ***P < 0.001 vs. WT group, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31297058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Construction of knockout (KO) & overexpression (OE) cell lines. (A) The sequencing results of KO positive clone cells; (B) The messenger RNA (mRNA) level of SREBP1 in wild type (WT) & OE Bel-7402 cells. Data were expressed as mean ± standard error of the mean. ***P < 0.001 vs. WT group; (C & D) The protein level of SREBP1 in WT, OE, & KO Bel-7402 cells. Data were expressed as mean ± standard error of the mean. ***P < 0.001 vs. WT group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31297058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - p75NTR stimulation activated SREBP1 & SREBP2 in Huh7 hepatocyte cells.Human Huh7 cells were stimulated with 10 ng/ml pro-NGF or 50 ng/ml NGF for 16 h followed by immunoblotting using antibodies to detect the presence of cleaved/activated SREBP1 & SREBP2. a SREBP1, immunoblots & b quantification. Values are mean ± SD, n = 3. **P < 0.01 & *p < 0.05 for treated vs. control cells. c, d SREBP2. Cells were treated with 10 ng/ml pro-NGF in the absence or presence of 0.5 μM PF429242 (PF), which is a selective inhibitor of the Site 1 protease (S1P). c Immunoblots & d quantification. PF decreased the amount of processed SREBP2 in these cells; however, addition of pro-NGF increased the relative level of cleaved SREBP2. Values are mean ± SD, n = 3. *p < 0.05 for treated vs. the corresponding control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31296846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Effect of GEN on the protein expression of Nrf2, ACC & mTORC signalling pathways in tyloxapol‐induced mice. GEN (50, 75, 100 mg/kg) was administered to WT or Nrf2−/− mice prior 1 h to stimulation of tyloxapol (500 mg/kg) for 18 h. A‐C, Protein expression of Nrf2, PPAR alpha , PPAR gamma in nucleus & HO‐1 in cytoplasm of WT mice was detected by Western blot. (D & E) Protein expression of Nrf2, PPAR alpha & PPAR gamma in Nrf2−/− mice was detected by Western blot. F‐H, Protein expression of P‐ACC, ACC, P‐AKT, AKT, P‐AMPK alpha , AMPK alpha , P‐AMPK beta , AMPK beta in WT & Nrf2−/− mice was detected by Western blot. I‐M, Protein expression of PI3K, P‐mTORC, mTORC, P‐S6K, S6K, P‐S6, S6, SREBP‐1c & HMGB1 WT & Nrf2−/− mice was detected by Western blot. The similar results were collected from three dependent experiments. All data were expressed by mean ± SEM (n = 5 in each group). ##P < .01 vs Control group; *P < .05 & **P < .01 vs Tyloxapol group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32293113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca, Ch, Ha, Pm, SyHa

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Ch, Ha, Pm, SyHa

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-582AF350
Alexa Fluor 405	NB600-582AF405
Alexa Fluor 488	NB600-582AF488
Alexa Fluor 532	NB600-582AF532
Alexa Fluor 594	NB600-582AF594
Alexa Fluor 647	NB600-582AF647
Alexa Fluor 700	NB600-582AF700
Alexa Fluor 750	NB600-582AF750
Allophycocyanin	NB600-582APC
Biotin	NB600-582B
CoraFluor 1	NB600-582CL1
DyLight 350	NB600-582UV
DyLight 405	NB600-582V
DyLight 488	NB600-582G
DyLight 550	NB600-582R
DyLight 594	NB600-582DL594
DyLight 650	NB600-582C
DyLight 680	NB600-582FR
DyLight 755	NB600-582IR
FITC	NB600-582F
HRP	NB600-582H
Janelia Fluor 525	NB600-582JF525
Janelia Fluor 549	NB600-582JF549
Janelia Fluor 646	NB600-582JF646
Janelia Fluor 669	NB600-582JF669
PE	NB600-582PE
PerCP	NB600-582PCP
mFluor Violet 450 SE	NB600-582MFV450
mFluor Violet 500 SE	NB600-582MFV500
mFluor Violet 610 SE	NB600-582MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-582SS
Azide and BSA Free	NBP2-80959

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NB100-2215

SREBP1 Antibody - BSA Free

(3 Reviews)

(59 Publications)

0.1 ml

NB100-2215

Western Blot: SREBP1 Antibody [NB100-2215] - MiR-181a overexpression impairs and miR-181a inhibition improves glucose and lipid homeostasis in HepG2 cellsHepG2 cells were divided into 4 groups as follows: a control group, mimics group (HepG2 cells transfected with 10 nM mimics), NEFA group (treated with 1. 2 mM NEFA), and miR-181a + NEFA group (transfected with 50 nM miR-181a inhibitors and then treated with 1. 2 mM NEFA). It was followed with or without 100 nM insulin. Immunoblot analysis (left) and quantification (right) of SREBP-1c and PPARI+- in HepG2 cells. Image collected and cropped by Citeab from the following publication (Upregulation of miR-181a impairs hepatic glucose and lipid homeostasis. Oncotarget (2017)) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-2215] - SREBP1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Nuclear and punctate vesicle staining was observed.

Immunohistochemistry-Paraffin: SREBP1 Antibody [NB100-2215] - nalysis of FFPE tissue section of mouse liver with SREBP1 antibody at 1:200. The antibody generated an expected cytoplasmic-nuclear immunostaining of SREBP1 protein in the hepatocytes.

Western Blot: SREBP1 Antibody [NB100-2215] - Detection of SREBP1. Lane 1: human liver. Lane 2: mouse liver.

Western Blot: SREBP1 Antibody - BSA Free [NB100-2215] - MiR-181a overexpression impairs & miR-181a inhibition improves glucose & lipid homeostasis in HepG2 cellsHepG2 cells were divided into 4 groups as follows: a control group, mimics group (HepG2 cells transfected with 10 nM mimics), NEFA group (treated with 1.2 mM NEFA), & miR-181a + NEFA group (transfected with 50 nM miR-181a inhibitors & then treated with 1.2 mM NEFA). B was followed with or without 100 nM insulin. (A) Immunoblot analysis (top) & quantification (bottom) of SIRT1, PGC-1 alpha & acetylated PGC-1 alpha in HepG2 cells. (B) Immunoblotting analysis (top) & quantification (bottom) of insulin-stimulated phosphorylation of AKT in HepG2 cells. (C) Glucose concentration in medium. (D) The glycogen content in HepG2 cells. (E) The mRNA expression levels of G6Pase & PEPCK. (F) Immunoblot analysis (left) & quantification (right) of SREBP-1c & PPAR alpha in HepG2 cells. (G) TG content in HepG2 cells. *P 25 (n = 24). There was no significant difference. (F) The BMI and H-scores of PPAR alpha , SREBP1, and FAS in each EMC patient are shown in scatter plots. Spearman’s rank correlation coefficient showed a weak inverse correlation between BMI and the PPAR alpha H-score and a moderate or weak correlation between BMI and SREBP1 or FAS. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37305395), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ha, Pl, ChHa

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ha, Pl, ChHa

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2215AF350
Alexa Fluor 405	NB100-2215AF405
Alexa Fluor 488	NB100-2215AF488
Alexa Fluor 532	NB100-2215AF532
Alexa Fluor 594	NB100-2215AF594
Alexa Fluor 647	NB100-2215AF647
Alexa Fluor 700	NB100-2215AF700
Alexa Fluor 750	NB100-2215AF750
Allophycocyanin	NB100-2215APC
Biotin	NB100-2215B
CoraFluor 1	NB100-2215CL1
DyLight 350	NB100-2215UV
DyLight 405	NB100-2215V
DyLight 488	NB100-2215G
DyLight 550	NB100-2215R
DyLight 594	NB100-2215DL594
DyLight 650	NB100-2215C
DyLight 680	NB100-2215FR
DyLight 755	NB100-2215IR
FITC	NB100-2215F
HRP	NB100-2215H
Janelia Fluor 525	NB100-2215JF525
Janelia Fluor 549	NB100-2215JF549
Janelia Fluor 646	NB100-2215JF646
Janelia Fluor 669	NB100-2215JF669
PE	NB100-2215PE
PerCP	NB100-2215PCP
mFluor Violet 450 SE	NB100-2215MFV450
mFluor Violet 500 SE	NB100-2215MFV500
mFluor Violet 610 SE	NB100-2215MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2215SS

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NBP1-49533

Glut4 Antibody - BSA Free

(4 Reviews)

(44 Publications)

0.1 ml

NBP1-49533

Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney

Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533 (NBP1-49533AF647) on mouse Thymic Epithelial cells. Glut4 antibody in comparison to negative and IgG Control. Primary antibody dilution: 1:500. Image from verified customer review.

Western Blot: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in A) MCF7 whole cell lysate and B) 3T3L1 whole cell lysate.

Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from Human HeLa and A431, Mouse 3T3 and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Glut4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.

Flow Cytometry: Glut4 Antibody [NBP1-49533] - GLUT4 Biotin/APC vs. [X] PE. Image from verified customer review.

<b>Biological Strategies Validation. </b>Flow Cytometry: Glut4 Antibody [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533. Staining of Glut 4 expression on Murine CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. This Alexa Fluor (R) 647 conjugated Glut 4 antibody (orange) positively stained mouse CD4+ T cells compared to Isotype Control (Rb IgG AF647, Novus NBP2-36463AF647, blue) and fluorescence minus one/FMO control (red). Image from verified customer review.

Flow (Intracellular): Glut4 Antibody [NBP1-49533] - An intracellular stain was performed on HepG2 with NBP1-49533 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of Glut4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of a FFPE tissue section of mouse small intestine using 1:200 dilution of Glut4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Reactivity:

Hu, Mu, Rt, Pl

Species Glossary

Reactivity: Hu, Mu, Rt, Pl

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-49533AF350
Alexa Fluor 405	NBP1-49533AF405
Alexa Fluor 488	NBP1-49533AF488
Alexa Fluor 532	NBP1-49533AF532
Alexa Fluor 594	NBP1-49533AF594
Alexa Fluor 647	NBP1-49533AF647
Alexa Fluor 700	NBP1-49533AF700
Alexa Fluor 750	NBP1-49533AF750
Allophycocyanin	NBP1-49533APC
Allophycocyanin/Cy7	NBP1-49533APCCY7
Biotin	NBP1-49533B
CoraFluor 1	NBP1-49533CL1
DyLight 350	NBP1-49533UV
DyLight 405	NBP1-49533V
DyLight 488	NBP1-49533G
DyLight 550	NBP1-49533R
DyLight 594	NBP1-49533DL594
DyLight 650	NBP1-49533C
DyLight 680	NBP1-49533FR
DyLight 755	NBP1-49533IR
FITC	NBP1-49533F
HRP	NBP1-49533H
Janelia Fluor 525	NBP1-49533JF525
Janelia Fluor 549	NBP1-49533JF549
Janelia Fluor 646	NBP1-49533JF646
Janelia Fluor 669	NBP1-49533JF669
PE	NBP1-49533PE
PE/Atto594	NBP1-49533PEATT594
PE/Cy5.5	NBP1-49533PECY55
PE/Cy7	NBP1-49533PECY7
PerCP	NBP1-49533PCP
mFluor Violet 450 SE	NBP1-49533MFV450
mFluor Violet 500 SE	NBP1-49533MFV500
mFluor Violet 610 SE	NBP1-49533MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-49533SS

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H00051099-M01

Abhd5 Antibody (1F3) - Azide and BSA Free

(1 Review)

(35 Publications)

0.1 mg

H00051099-M01

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Western Blot analysis of ABHD5 expression in Jurkat(Cat # L017V1 ).

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Western Blot analysis of ABHD5 expression in human liver.

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 monoclonal antibody (M01), clone 1F3. Analysis of ABHD5 expression in HeLa.

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 monoclonal antibody (M01), clone 1F3. Analysis of ABHD5 expression in rat testis.

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody (M01), clone 1F3.Lane 1: ABHD5 transfected lysate(39 KDa).Lane 2: Non-transfected lysate.

ELISA: Abhd5 Antibody (1F3) [H00051099-M01] - Detection limit for recombinant GST tagged ABHD5 is approximately 1ng/ml as a capture antibody.

The Role of MAGL and CGI-58 in IL-6 and beta -adrenergic Adipocyte Signaling and Lipolysis. (A-D) Pre-adipocytes in a 12-well format were infected with lentivirus containing Cas9 and sgGFP (black), sgAbhd5 (red), or sgMgll (blue) followed by differentiation as described in Methods. On day 8, differentiated adipocytes were treated in a final volume of 1 ml of medium D with 3 ul DMSO and 3 ul PBS containing the indicated concentration of isoproterenol (A, B), IL-6 (A, C), or LIF (A, D). After incubation for 24 hours, medium was collected and processed to measure concentrations of glycerol (left panel) or NEFA (right panel) and adipocytes were harvested and processed for immunoblot analysis (A) with the indicated antibodies as described in Methods. Glycerol levels are shown relative to the average 300 nM isoproterenol condition (B, 653 uM), 3 nM IL-6 condition (C, 109 uM), or 0.3 nM LIF condition (D, 55 uM) of the sgGFP-treated adipocytes. NEFA levels are shown relative to the average 300 nM isoproterenol condition (B, 282 uM), 3 nM IL-6 condition (C, 70 uM), or 1 nM LIF condition (D, 21 uM) of the sgGFP-treated adipocytes. Data is shown as mean +/- SEM of three wells. P value calculated by using a two-way repeated measures ANOVA between sgGFP and the indicated sgRNA conditions. These results were confirmed in at least three independent experiments. Not significant (n.s.). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35785158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Comparison of IL-6 Family Cytokine and beta -adrenergic Adipocyte Signaling and Lipolysis. (A–D) Differentiated adipocytes in a 12-well format were treated in a final volume of 1 ml of medium D with 3 ul DMSO and 3 ul PBS in the absence or presence of 300 nM isoproterenol (black, Isop.), 3 nM IL-6 (red), or 0.3 nM LIF (blue). After incubation (37C, 10% CO2) for either 24 hours (A, B) or the indicated time (C, D), medium was collected and processed to measure concentrations of NEFA (A, C; top panels) or glycerol (A,C; bottom panels), and adipocytes were harvested and processed for immunoblot analysis (B, D) with the indicated antibody as described in Methods. Data is shown as mean +/- SEM of three wells. *p < 0.05, **p < 0.01, or ****p < 0.0001 based on one-way ANOVA followed by Dunnett’s multiple comparison test to compare each condition to the vehicle condition (A) or two-way ANOVA followed by Šidak’s multiple comparison test to compare isoproterenol to IL-6 conditions at matched time points (B). These results were confirmed in at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35785158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The Role of ATGL and HSL in IL-6 and beta -adrenergic Adipocyte Signaling and Lipolysis. (A–D) Pre-adipocytes in a 12-well format were infected with lentivirus containing Cas9 and sgGFP (black), sgPnpla2 (red), or sgLipe (blue) followed by differentiation as described in Methods. On day 8, differentiated adipocytes were treated in a final volume of 1 ml of medium D with 3 ul DMSO and 3 ul PBS containing the indicated concentration of isoproterenol (A, B), IL-6 (A, C), or LIF (A, D). After incubation for 24 hours, medium was collected and processed to measure concentrations of glycerol (left panel) or NEFA (right panel) and adipocytes were harvested and processed for immunoblot analysis (A) with the indicated antibodies as described in Methods. Glycerol levels are shown relative to the average 300 nM isoproterenol condition (B, 578 uM), 3 nM IL-6 condition (C, 140 uM), or 1 nM LIF condition (D, 86 uM) of the sgGFP-treated adipocytes. NEFA levels are shown relative to the average 300 nM isoproterenol condition (B, 123 uM), 3 nM IL-6 condition (C, 57 uM), or 1 nM LIF condition (D, 27 uM) of the sgGFP-treated adipocytes. Data is shown as mean +/- SEM of three wells. P value calculated by using a two-way repeated measures ANOVA between sgGFP and the indicated sgRNA conditions. These results were confirmed in at least three independent experiments. Not significant (n.s.). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35785158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The Role of STAT3 in IL-6 and beta -adrenergic Adipocyte Signaling and Lipolysis. (A–C) Pre-adipocytes in a 12-well format were infected with lentivirus containing Cas9 and the indicated sgRNA as described in Methods. On day 8, differentiated adipocytes were treated in a final volume of 1 ml of medium D with 3 ul DMSO and 3 ul PBS containing 300 nM isoproterenol, 3 nM IL-6, or 0.3nM LIF. After incubation for 24 hours, medium was collected and processed to measure concentrations of glycerol (B) or NEFA (C) and adipocytes were harvested and processed for immunoblot analysis (A) with the indicated antibodies as described in Methods. Glycerol release is shown relative to the respective stimulant in the sgGFP condition which were 366 μM, 70 μM, and 33 μM for isoproterenol, IL-6, and LIF, respectively. NEFA release is shown relative to the respective stimulant in the sgGFP condition which were 273 μM, 137 μM, and 34 μM for isoproterenol, IL-6, and LIF, respectively. Data is shown as mean +/- SEM of three wells. *p < 0.5, ***p < 0.001, or ****p < 0.0001 based on two-way ANOVA with Tukey’s multiple comparison test for differences in relative glycerol or NEFA release between sgRNA treatments with the same stimulant. These results were confirmed in at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35785158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NB400-114

Fatty Acid Synthase/FASN Antibody - BSA Free

(2 Reviews)

(29 Publications)

0.1 ml

NB400-114

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA and 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11320), licensed under a CC-BY license.

<b>Genetic Strategies Validation. </b>Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Western blot showing silencing efficiency of FASN-, ACC- and ACLY-specific siRNAs on CHIKV replication (n=10 for each data set).

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle.(a) Impact of FASN or ACLY knockdown on CHIKV replication. Closed & open symbols indicate replicates from the primary screen & during validation, respectively. (b) Western blot showing silencing efficiency of siRNAs used in c. (c) Impact of FASN-, ACC- & ACLY-specific siRNAs on CHIKV replication (n=10 for each data set). (d) Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA & 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 μm. (e) Co-localization analysis of cells labelled as in d & in Supplementary Fig. 3b, plotted as Pearson's coefficient per cell. Each symbol corresponds to a cell stack from three independent experiments (n=29 cells for FASN, 30 cells for ACC & 31 cells for ACLY); median values shown in red. (f) Effect of FASN (cerulenin, n=12 for each data set), ACC (TOFA, n=11 for each data set) & ACLY (BMS-303141 n=11 for each data set) inhibitors on CHIKV replication. (g) Real-time cell toxicity assay performed on HeLa cells (n=3 for each point). Excepted for b & d where representative images are shown & for g where the mean±s.d. is shown for each point of a representative experiment, all data represent the means±s.e.m. of three independent experiments analysed using one-way analysis of variance with Tukey's post test (*P<0.05; **P<0.01; ***P<0.001; NSP≥0.05). NS, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27177310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Fatty acid synthesis requirement for CHIKV life cycle.(a) Impact of FASN or ACLY knockdown on CHIKV replication. Closed and open symbols indicate replicates from the primary screen and during validation, respectively. (b) Western blot showing silencing efficiency of siRNAs used in c. (c) Impact of FASN-, ACC- and ACLY-specific siRNAs on CHIKV replication (n=10 for each data set). (d) Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA and 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 μm. (e) Co-localization analysis of cells labelled as in d and in Supplementary Fig. 3b, plotted as Pearson's coefficient per cell. Each symbol corresponds to a cell stack from three independent experiments (n=29 cells for FASN, 30 cells for ACC and 31 cells for ACLY); median values shown in red. (f) Effect of FASN (cerulenin, n=12 for each data set), ACC (TOFA, n=11 for each data set) and ACLY (BMS-303141 n=11 for each data set) inhibitors on CHIKV replication. (g) Real-time cell toxicity assay performed on HeLa cells (n=3 for each point). Excepted for b and d where representative images are shown and for g where the mean+/-s.d. is shown for each point of a representative experiment, all data represent the means+/-s.e.m. of three independent experiments analysed using one-way analysis of variance with Tukey's post test (*P<0.05; **P<0.01; ***P<0.001; NSP≥0.05). NS, not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27177310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB400-114SS

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NB110-41576

Abhd5 Antibody - BSA Free

(29 Publications)

0.1 ml

NB110-41576

Western Blot: Abhd5 Antibody [NB110-41576] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Protein abundance of ATGL. In all figure panels data are expressed relative to a no fatty acid control condition (0mM). In panel B, #P<0.05 for a main effect of HSFA vs. PALM and NORM. Representative blots are inset above each figure panel. GPAT, glycerol-3-phosphate acyltransferase; DGAT, diacylglycerol acyltransferase; ATGL, adipose triacylglycerol lipase; CGI-58, comparative gene identification 58; HSL, hormone sensitive lipase; AU, arbitrary units. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120871), licensed under a CC-BY license.

Western Blot: Abhd5 Antibody [NB110-41576] - MCF7 cell lysates.

Simple Western: Abhd5 Antibody [NB110-41576] - Image shows a specific band for Abhd5 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Protein content of important players involved in lipid droplet dynamics in Caucasian and South Asian subjects before and after a 5-day HFHC-diet.(a) ATGL, (b) CGI-58, (c) PLIN2, (d) PLIN3 and (e) PLIN5 protein content. Data are presented as mean ± SEM and were statistically analyzed with a Repeated Measures ANOVA; *P < 0.05 for diet effect, #P < 0.05 for group effect.

The turnover of DAG and TAG is increased in the DGAT1 overexpressing TA muscle.(A) Western blotting of ATGL, (B) CGI58 (C) and ADRP in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). # P<0.05 HFD-DGAT1 vs. HFD-control.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - A. ATGL (n = 8) & CGI-58 (n = 7) protein content in the three skeletal muscles (SOL, RG, & WG & heart).Ponceau stain for total protein was used as a loading control. Data are reported as mean±SE & bars with the same letter are not significantly different within the skeletal muscles (p<0.05). Insets: representative blots: lane 1, WG (white gastrocnemius); lane 2, RG (red gastrocnemius); lane 3, SOL (soleus). B. ATGL & CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - The turnover of DAG & TAG is increased in the DGAT1 overexpressing TA muscle.(A) Western blotting of ATGL, (B) CGI58 (C) & ADRP in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). # P<0.05 HFD-DGAT1 vs. HFD-control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Effects of treadmill running on the mRNA & protein expressions of ATGL (A,E), CGI-58 (B,F), G0S2 (C,G), HSL (D,H) in the right ventricle. Data are expressed as mean ± SEM. For the sake of clarity, the control group was set at 100, & exercised groups were scaled with respect to Ctrl * p < 0.05 difference vs. control (Ctrl); #p < 0.05 difference M120 vs. M30; $p < 0.05 difference F30 vs. M30. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31137663), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Protein content of important players involved in lipid droplet dynamics in Caucasian & South Asian subjects before & after a 5-day HFHC-diet.(a) ATGL, (b) CGI-58, (c) PLIN2, (d) PLIN3 & (e) PLIN5 protein content. Data are presented as mean ± SEM & were statistically analyzed with a Repeated Measures ANOVA; *P < 0.05 for diet effect, #P < 0.05 for group effect. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep42393), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-41576AF350
Alexa Fluor 405	NB110-41576AF405
Alexa Fluor 488	NB110-41576AF488
Alexa Fluor 532	NB110-41576AF532
Alexa Fluor 594	NB110-41576AF594
Alexa Fluor 647	NB110-41576AF647
Alexa Fluor 700	NB110-41576AF700
Alexa Fluor 750	NB110-41576AF750
Allophycocyanin	NB110-41576APC
Biotin	NB110-41576B
CoraFluor 1	NB110-41576CL1
DyLight 350	NB110-41576UV
DyLight 405	NB110-41576V
DyLight 488	NB110-41576G
DyLight 550	NB110-41576R
DyLight 594	NB110-41576DL594
DyLight 650	NB110-41576C
DyLight 680	NB110-41576FR
DyLight 755	NB110-41576IR
FITC	NB110-41576F
HRP	NB110-41576H
Janelia Fluor 525	NB110-41576JF525
Janelia Fluor 549	NB110-41576JF549
Janelia Fluor 646	NB110-41576JF646
Janelia Fluor 669	NB110-41576JF669
PE	NB110-41576PE
PerCP	NB110-41576PCP
mFluor Violet 450 SE	NB110-41576MFV450
mFluor Violet 500 SE	NB110-41576MFV500
mFluor Violet 610 SE	NB110-41576MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-41576SS

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NB110-41487

DGAT1 Antibody - BSA Free

(1 Review)

(27 Publications)

0.1 ml

NB110-41487

Western Blot: DGAT1 Antibody [NB110-41487] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Pictured is protein abundance of DGAT1. In the figure panel, the data is expressed relative to a no fatty acid control condition (0mM). In panel B, #P<0.05 for a main effect of HSFA vs. PALM and NORM. Representative blot shown inset above the figure panel. DGAT = diacylglycerol acyltransferase; AU = arbitrary units. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120871), licensed under a CC-BY license.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-41487AF350
Alexa Fluor 405	NB110-41487AF405
Alexa Fluor 488	NB110-41487AF488
Alexa Fluor 532	NB110-41487AF532
Alexa Fluor 594	NB110-41487AF594
Alexa Fluor 647	NB110-41487AF647
Alexa Fluor 700	NB110-41487AF700
Alexa Fluor 750	NB110-41487AF750
Allophycocyanin	NB110-41487APC
Biotin	NB110-41487B
CoraFluor 1	NB110-41487CL1
DyLight 350	NB110-41487UV
DyLight 405	NB110-41487V
DyLight 488	NB110-41487G
DyLight 550	NB110-41487R
DyLight 594	NB110-41487DL594
DyLight 650	NB110-41487C
DyLight 680	NB110-41487FR
DyLight 755	NB110-41487IR
FITC	NB110-41487F
HRP	NB110-41487H
Janelia Fluor 525	NB110-41487JF525
Janelia Fluor 549	NB110-41487JF549
Janelia Fluor 646	NB110-41487JF646
Janelia Fluor 669	NB110-41487JF669
PE	NB110-41487PE
PerCP	NB110-41487PCP
mFluor Violet 450 SE	NB110-41487MFV450
mFluor Violet 500 SE	NB110-41487MFV500
mFluor Violet 610 SE	NB110-41487MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-41487SS

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NB300-537

PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free

(1 Review)

(17 Publications)

100 μg

NB300-537

Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Hek293 cells with PPAR alpha/NR1C1 [3B6/PPAR] Antibody NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.

Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Jurkat cells compared to an isotype control (blue).

Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Hela cells compared to an isotype control (blue).

Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in 3T3 cells compared to an isotype control (blue).

Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

<b>Genetic Strategies Validation. </b>Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free [NB300-537] - PPAR alpha/NR1C1 was knocked down with siRNA in primary cultured rat cardiomyocytes. Image from verified customer review.

The metabolic remodeling process in natural kidney aging. A Representative images of Oil Red O staining in the 24-month-old and 6-month-old group (n = 6), scale bar = 50 μm; B, C the downregulated protein levels of FAO markers (i.e., PPAR alpha , ACOX1, and CPT1A) and upregulated protein levels of glycolysis markers (i.e., HK2 and PDK1) by western blot, and their semi-quantitative analyses (n = 6); D immunostaining for GLIS1 (red) and PPAR alpha (green), with DAPI (blue) counterstaining by IF staining in the 24-month-old and 6-month-old group (n = 6), scale bar = 50 μm; E lactate levels in the 24-month-old and 6-month-old group (n = 6); F the downregulated protein levels of PPAR alpha , ACOX1, and CPT1A, and up-regulated protein levels of HK2 and PDK1 in the 24-month-old group by IHC assay and their semi-quantitative analyses (n = 6), scale bar = 50 μm. The data are expressed as the mean +/- SD of three independent experiments. **P < .01 or ***P < .001 versus the 6-month-old group by Student’s t-test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39736678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Bioinformatics analysis of proteomic results.(A). Hierarchical clustering analysis of the differentially expressed proteins in the two groups (si-CD151 vs si-NC) (n = 3) (B) The differentially expressed proteins analyzed by volcano plots between every two groups (si-CD151 vs si-NC) (C) Circus plots of GO Classification Annotation of differential protein. (D) KEGG pathways of differentially expressed proteins. OS: Organismal Systems; M: Metabolism; GIP: Genetic Information Processing; CP: Cellular Processes. (E) Western blot analysis of PPAR-alpha , PCG-1 alpha and GAPDH) in CMs under PE, quantified by Image J (n = 4). (F) Western blot analysis of PPAR-gamma and GAPDH) in CFs under Ang-II, quantified by Image J (n = 4). Data are expressed as mean +/- SEM. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0297121), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The over-expressed GLIS1 suppressed metabolic remodeling from FAO to glycolysis in the accelerated aging mouse model. A The representative images of mouse kidney tissue in the control, AAV-Vector and AAV-GLIS1 group stained with Oil Red O (n = 6), scale bar = 50 μm; B, C downregulated protein levels of PPAR alpha , ACOX1, and CPT1A in the accelerated aging mouse model were reversed in the presence of AAV-GLIS1, while upregulated protein levels of HK2 and PKD1 in the accelerated aging mouse model were reduced by introducing AAV-GLIS1 (n = 6); D lactate levels in the control, AAV-Vector and AAV-GLIS1 group (n = 6); E the reversed effect of PPAR alpha , ACOX1 and CPT1A levels, as well as HK2 and PDK1 levels in the presence of AAV-GLIS1 by IHC assay, and their semi-quantitative analyses (n = 6), scale bar = 50 μm; F immunostaining for PPAR alpha , ACOX1, CPTA1, HK2, and PDK1 (green), with DAPI (blue) counterstaining by IF staining in accelerated aging mouse model (n = 6), scale bar = 50 μm. The data are expressed as the mean +/- SD of three independent experiments. **P < .01 or ***P < .001 versus the AAV-vector group by one-way ANOVA Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39736678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The ablation of METTL3 triggered metabolic remodeling and aggravated cell senescence renal fibrosis. A Protein levels of GLIS1, PPAR alpha , ACOX1, CPT1A, HK2, PDK1, FN, alpha -SMA, and P16INK4A by western blot in the vector and siMETTL3 group, and their semi-quantitative analyses (n = 3); B representative images of Oil Red O staining in the vector and siMETTL3 group (n = 3), scale bar = 50 μm; C double immunostaining for GLS1 and PPAR alpha , ACOX1, CPT1A, HK2, PDK1, and alpha -SMA by IF staining (n = 3) scale bar = 50 μm. The data are expressed as the mean +/- SD of three independent experiments. **P < .01 or ***P < .001 versus the vector group by Student’s t-test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39736678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

CCM treatment upregulates AMPK and PPAR-alpha pathways. (a) Western blot analysis of the expression of AMPK. Densitometric analysis is shown in the bar graph. (b) Western blot analysis of the expressions of PPAR-alpha and PGC-1 alpha . Densitometric analysis is shown in the bar graph. Data are expressed as mean values +/- SD (*P < 0.05 compared with the sham group; #P < 0.05 compared with HF group). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35799598), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Rb

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-537AF350
Alexa Fluor 405	NB300-537AF405
Alexa Fluor 488	NB300-537AF488
Alexa Fluor 532	NB300-537AF532
Alexa Fluor 594	NB300-537AF594
Alexa Fluor 647	NB300-537AF647
Alexa Fluor 700	NB300-537AF700
Alexa Fluor 750	NB300-537AF750
Allophycocyanin	NB300-537APC
Biotin	NB300-537B
CoraFluor 1	NB300-537CL1
DyLight 350	NB300-537UV
DyLight 405	NB300-537V
DyLight 488	NB300-537G
DyLight 550	NB300-537R
DyLight 594	NB300-537DL594
DyLight 650	NB300-537C
DyLight 680	NB300-537FR
DyLight 755	NB300-537IR
FITC	NB300-537F
HRP	NB300-537H
Janelia Fluor 525	NB300-537JF525
Janelia Fluor 549	NB300-537JF549
Janelia Fluor 646	NB300-537JF646
Janelia Fluor 669	NB300-537JF669
PE	NB300-537PE
PerCP	NB300-537PCP
mFluor Violet 450 SE	NB300-537MFV450
mFluor Violet 500 SE	NB300-537MFV500
mFluor Violet 610 SE	NB300-537MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-537-0.025mg

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NB100-125

PER2 Antibody - BSA Free

(3 Reviews)

(13 Publications)

0.1 ml

NB100-125

Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Deletion of IL-1 beta signaling ameliorates expression of hepatic circadian transcription factors following DSS-PN.8–10 week old IL1KO mice were subjected to Chow feeding or DSS-PN. A. Liver tissue was harvested, and mRNA expression of transcription factors regulating hepatic CR analyzed by qRT-PCR. Expression was normalized against HPRT. Values are Mean+/- SEM, N = at least 3/condition. *p<0.05. **p<0.01, ***P<0.001, ****P<0.0001. B. Western analysis of circadian regulatory proteins. Expression was normalized using Gapdh expression for each blot. Values are Mean+/- SEM, N = 3/condition, a = significantly different from respective chow control, b = significantly different compared to WT DSS-PN. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37647292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Genetic inhibition of TNF alpha signaling normalizes expression of hepatic circadian transcription factors following DSS-PN.8–10 week old TNFRKO mice were subjected to Chow feeding or DSS-PN. A. Liver tissue was harvested, and mRNA expression of transcription factors regulating hepatic CR analyzed by qRT-PCR. Expression was normalized against HPRT. N = 4 per condition. Values are Mean+/- SEM. *p<0.05. **p<0.01, ***p<0.001. B. Western analysis of circadian regulatory proteins. Expression was normalized using Gapdh expression for each blot. Values are Mean+/- SEM, N = 3/condition, a = significantly different from respective chow control, b = significantly different compared to WT DSS-PN. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37647292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-125AF350
Alexa Fluor 405	NB100-125AF405
Alexa Fluor 488	NB100-125AF488
Alexa Fluor 532	NB100-125AF532
Alexa Fluor 594	NB100-125AF594
Alexa Fluor 647	NB100-125AF647
Alexa Fluor 700	NB100-125AF700
Alexa Fluor 750	NB100-125AF750
Allophycocyanin	NB100-125APC
Biotin	NB100-125B
CoraFluor 1	NB100-125CL1
DyLight 350	NB100-125UV
DyLight 405	NB100-125V
DyLight 488	NB100-125G
DyLight 550	NB100-125R
DyLight 594	NB100-125DL594
DyLight 650	NB100-125C
DyLight 680	NB100-125FR
DyLight 755	NB100-125IR
FITC	NB100-125F
HRP	NB100-125H
Janelia Fluor 525	NB100-125JF525
Janelia Fluor 549	NB100-125JF549
Janelia Fluor 646	NB100-125JF646
Janelia Fluor 669	NB100-125JF669
PE	NB100-125PE
PerCP	NB100-125PCP
mFluor Violet 450 SE	NB100-125MFV450
mFluor Violet 500 SE	NB100-125MFV500
mFluor Violet 610 SE	NB100-125MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-125SS

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NBP1-07047

PDK4 Antibody - BSA Free

(2 Reviews)

(15 Publications)

0.1 ml

NBP1-07047

Western Blot: PDK4 Antibody [NBP1-07047] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. * = vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.

Western Blot: PDK4 Antibody - BSA Free [NBP1-07047] - Genetic manipulations can alter PDH E1 alpha phosphorylation in response to hypoxia.(a) MIA PaCa-2 control (Hg), silenced HIF1 alpha (shHIF1 alpha ) or silenced PDHK1 (shPDHK1) & PDP1 overexpressing cells were incubated for 16 h in normoxia or hypoxia (0.5% O2) & phospho-serine E1 alpha detected by Western blot showing that all three modifications can have an inhibitory effect on hypoxic Ser232 phosphorylation. (b) Increased pSer232-E1 alpha detection after E1 alpha immunoprecipitation from lysates of cells treated as in (a). (c) The same analysis as in (a) after treatment with 16 h 1 mM DMOG. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27498883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PDK4 Antibody - BSA Free [NBP1-07047] - Hypoxia inhibits mitochondrial OCR & PDH activity & induces PDHK1 protein & activity.(a) Ratio of oxygen consumption rate (OCR) to extracellular acidification rate (ECAR) measured by Seahorse XF in MIA PaCa-2, PANC-1, & SU.86.86 cell lines in high (25 mM) or low (0.5 mM) glucose incubated overnight with or without 1 mM DMOG. (mean ± SEM, two-tailed Student’s t-test, **p < 0.01, ***p < 0.001) (b) Cell-based PDH activity assay in cells incubated 16 h in normoxia, hypoxia (0.5% O2) or 1 mM DMOG. (mean ± SD, one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001) (c) Western blots of HIF alpha isoforms, pyruvate dehydrogenase kinase isoforms (PDHKs), phosphatase (PDP1 – lower band *), target phosphorylated serine residues on E1 alpha & total E1 alpha after overnight incubation in normoxia or hypoxia (0.5% O2) at 25 or 5 mM glucose as indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27498883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-07047AF350
Alexa Fluor 405	NBP1-07047AF405
Alexa Fluor 488	NBP1-07047AF488
Alexa Fluor 532	NBP1-07047AF532
Alexa Fluor 594	NBP1-07047AF594
Alexa Fluor 647	NBP1-07047AF647
Alexa Fluor 700	NBP1-07047AF700
Alexa Fluor 750	NBP1-07047AF750
Allophycocyanin	NBP1-07047APC
Biotin	NBP1-07047B
CoraFluor 1	NBP1-07047CL1
DyLight 350	NBP1-07047UV
DyLight 405	NBP1-07047V
DyLight 488	NBP1-07047G
DyLight 550	NBP1-07047R
DyLight 594	NBP1-07047DL594
DyLight 650	NBP1-07047C
DyLight 680	NBP1-07047FR
DyLight 755	NBP1-07047IR
FITC	NBP1-07047F
HRP	NBP1-07047H
Janelia Fluor 525	NBP1-07047JF525
Janelia Fluor 549	NBP1-07047JF549
Janelia Fluor 646	NBP1-07047JF646
Janelia Fluor 669	NBP1-07047JF669
PE	NBP1-07047PE
PerCP	NBP1-07047PCP
mFluor Violet 450 SE	NBP1-07047MFV450
mFluor Violet 500 SE	NBP1-07047MFV500
mFluor Violet 610 SE	NBP1-07047MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-07047SS

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

PGC1 alpha Antibody - BSA Free

BMAL1 Antibody - BSA Free

SREBP1 Antibody (2A4) - BSA Free

SREBP1 Antibody - BSA Free

Glut4 Antibody - BSA Free

Abhd5 Antibody (1F3) - Azide and BSA Free

Fatty Acid Synthase/FASN Antibody - BSA Free

Abhd5 Antibody - BSA Free

DGAT1 Antibody - BSA Free

PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free

PER2 Antibody - BSA Free

PDK4 Antibody - BSA Free