PER2 Antibody

Images

 
Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.
Immunocytochemistry/ Immunofluorescence: PER2 Antibody [NB100-125] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated ...read more
Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more
Immunocytochemistry/ Immunofluorescence: PER2 Antibody [NB100-125] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PER2 Antibody Summary

Immunogen
A partial synthetic peptide made to an internal portion of the human PER2 protein (between amino acids 100-150) [UniProt# O15055].
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PER2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
Application Notes
This PER2 antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. It has been tested against expressed fragment of human PER2 (bacterially expressed fragment from BL21 cells), or full-length protein expressed in in vitro translation using rabbit reticulocyte lysate. In Western blot, a band can be seen at ~200 kDa representing PER2. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID:31822134). Use in immunoprecipitation reported in scientific literature (PMID:31390562).
Reviewed Applications
Read 3 Reviews rated 3.7
using
NB100-125 in the following applications:

Publications
Read Publications using
NB100-125 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Peptide 1 has 94% sequence identity to rat. Peptide 2 has 94% sequence identity to rat and mouse. Mouse reactivity reported in scientific literature (PMID: 30016666). Use in Rat reported in scientific literature (PMID:32119846).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PER2 Antibody

  • Circadian clock protein PERIOD 2
  • FASPS
  • hPER2
  • KIAA0347period 2
  • period (Drosophila) homolog 2
  • period circadian protein 2
  • period circadian protein homolog 2
  • period homolog 2 (Drosophila)

Background

PER2 (period circadian protein homolog 2 or circadian clock protein PERIOD 2) is a component of the circadian clock mechanism which is essential for generating circadian rhythms and PER2 acts as a negative element in the circadian transcriptional loop. PER2 modulates the clock function by interacting with other circadian regulatory proteins for transporting them to nucleus, and negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. PER2 forms a component of the circadian core oscillator, which also includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS proteinsetc. PER2 interacts with NFIL3, PER1, PER3, CRY1 and CRY2 proteins, and its interaction with CSNK1D or CSNK1E promotes nuclear localization of PER proteins. PER2 is a nuclear protein and its nucleo-cytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. CSNK1E and CSNK1D facilitate PER2 phosphorylation that follows its degradation. PER2 defects have been found to be responsible for familial advanced sleep-phase syndrome (FASPS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PER2 Antibody (NB100-125)(5)

Reviews for PER2 Antibody (NB100-125) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB100-125:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot PER2 NB100-125
Enlarge
2
reviewed by:
Katharina Sahiri
WB Human 02/21/2019
View

Summary

ApplicationWestern Blot
Sample TestedU-2 OS cells
SpeciesHuman
LotE

Comments

Commentsunspecific bands with no obvious band of correct size
Western Blot PER2 NB100-125
Enlarge
5
reviewed by:
Anonymous
WB Human 11/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell line
SpeciesHuman
LotD-1

Comments

CommentsSample: Whole cell lysates of cancer cell lines (10ug)
Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
Primary Antibody diluted (1:500) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
Western Blot PER2 NB100-125
Enlarge
4
reviewed by:
Anonymous
WB Human 12/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates of various cancer cell lines
SpeciesHuman
LotC-1

Blocking

Blocking DetailsBlocked in 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature

Primary Anitbody

Dilution RatioDiluted in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG (H&L) HRP-conjugated antibody
Secondary Manufacturer Cat#Cell Signaling #7074
Secondary Concentration1:2000

Details

Detection NotesDetected using Pierce ECL Western Blotting Substrate, exposing for 1 min, imaged on Amersham LAS 4010

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PER2 Antibody (NB100-125). (Showing 1 - 1 of 1 FAQs).

  1. Would you please let me know if there is any updated data available regarding the testing of this antibody on mouse by WB?
    • We do not have an image showing the detection of NB100-125 in mouse tissues, however we will 100% guarantee this item to work as stated on the datasheet.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-125AF350
Alexa Fluor 405 NB100-125AF405
Alexa Fluor 488 NB100-125AF488
Alexa Fluor 532 NB100-125AF532
Alexa Fluor 594 NB100-125AF594
Alexa Fluor 647 NB100-125AF647
Alexa Fluor 700 NB100-125AF700
Alexa Fluor 750 NB100-125AF750
Biotin NB100-125B
DyLight 350 NB100-125UV
DyLight 405 NB100-125V
DyLight 488 NB100-125G
DyLight 550 NB100-125R
DyLight 594 NB100-125DL594
DyLight 650 NB100-125C
DyLight 680 NB100-125FR
DyLight 755 NB100-125IR
FITC NB100-125F
HRP NB100-125H
Janelia Fluor 549 NB100-125JF549
Janelia Fluor 646 NB100-125JF646

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Array NB100-125

Bioinformatics Tool for PER2 Antibody (NB100-125)

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Calculators

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Recent Reviews

3.7
5
1
4
1
3
0
2
1
1
0

Katharina Sahiri
02/21/2019
Application: WB
Species: Human

Anonymous
11/12/2017
Application: WB
Species: Human

Anonymous
12/10/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PER2
Entrez
Uniprot