PER2 Antibody

Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PER2 Antibody Summary

• Immunogen: Two peptides derived from human PER2 [UniProt# O15055]. Antibodies cross-reacting with each peptide were affinity purified against the appropriate peptide.
• Localization: Nuclear
• Predicted Species: Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PER2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
• Application Notes: This PER2 antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. It has been tested against expressed fragment of human PER2 (bacterially expressed fragment from BL21 cells), or full-length protein expressed in in vitro translation using rabbit reticulocyte lysate. In Western blot, a band can be seen at ~200 kDa representing PER2.

Reactivity Notes

Human. Peptide 1 has 94% homology to rat. Peptide 2 has 94% homology to rat and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for PER2 Antibody

• Circadian clock protein PERIOD 2
• FASPS
• hPER2
• KIAA0347period 2
• period (Drosophila) homolog 2
• period circadian protein 2
• period circadian protein homolog 2
• period homolog 2 (Drosophila)

Background

PER2 (period circadian protein homolog 2 or circadian clock protein PERIOD 2) is a component of the circadian clock mechanism which is essential for generating circadian rhythms and PER2 acts as a negative element in the circadian transcriptional loop. PER2 modulates the clock function by interacting with other circadian regulatory proteins for transporting them to nucleus, and negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. PER2 forms a component of the circadian core oscillator, which also includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS proteinsetc. PER2 interacts with NFIL3, PER1, PER3, CRY1 and CRY2 proteins, and its interaction with CSNK1D or CSNK1E promotes nuclear localization of PER proteins. PER2 is a nuclear protein and its nucleo-cytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. CSNK1E and CSNK1D facilitate PER2 phosphorylation that follows its degradation. PER2 defects have been found to be responsible for familial advanced sleep-phase syndrome (FASPS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PER2 Antibody (NB100-125)(1)

Publication using NB100-125

• Lee Y, Jang AR, Francey LJ et al. KPNB1 mediates PER/CRY nuclear translocation and circadian clock function. Elife 2015 [PMID: 26319354] (WB)

Reviews for PER2 Antibody (NB100-125) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous WB Human 11/12/2017

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell line
• Species: Human
• Lot: D-1

Comments

• Comments: Sample: Whole cell lysates of cancer cell lines (10ug)
  Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
  Primary Antibody diluted (1:500) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
  Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T

reviewed by: Anonymous WB Human 12/10/2014

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysates of various cancer cell lines
• Species: Human
• Lot: C-1

Blocking

• Blocking Details: Blocked in 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature

Primary Anitbody

• Dilution Ratio: Diluted in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG (H&L) HRP-conjugated antibody
• Secondary Manufacturer Cat#: Cell Signaling #7074
• Secondary Concentration: 1:2000

Details

• Detection Notes: Detected using Pierce ECL Western Blotting Substrate, exposing for 1 min, imaged on Amersham LAS 4010

FAQs for PER2 Antibody (NB100-125). (Showing 1 - 1 of 1 FAQ).

1. Would you please let me know if there is any updated data available regarding the testing of this antibody on mouse by WB?
   • We do not have an image showing the detection of NB100-125 in mouse tissues, however we will 100% guarantee this item to work as stated on the datasheet.

Bioinformatics

• Gene Symbol: PER2
• Entrez:
  • 8864(Human)
• Uniprot:
  • O15055(Human)