PER2 Antibody

Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PER2 Antibody Summary

• Immunogen: A partial synthetic peptide made to an internal portion of the human PER2 protein (between amino acids 100-150) [UniProt# O15055].
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PER2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
• Application Notes: This PER2 antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. It has been tested against expressed fragment of human PER2 (bacterially expressed fragment from BL21 cells), or full-length protein expressed in in vitro translation using rabbit reticulocyte lysate. In Western blot, a band can be seen at ~200 kDa representing PER2. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID:31822134). Use in immunoprecipitation reported in scientific literature (PMID:31390562).

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Peptide 1 has 94% sequence identity to rat. Peptide 2 has 94% sequence identity to rat and mouse. Mouse reactivity reported in scientific literature (PMID: 30016666). Use in Rat reported in scientific literature (PMID:32119846).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PER2 Antibody

• Circadian clock protein PERIOD 2
• FASPS
• hPER2
• KIAA0347period 2
• period (Drosophila) homolog 2
• period circadian protein 2
• period circadian protein homolog 2
• period homolog 2 (Drosophila)

Background

PER2 (period circadian protein homolog 2 or circadian clock protein PERIOD 2) is a component of the circadian clock mechanism which is essential for generating circadian rhythms and PER2 acts as a negative element in the circadian transcriptional loop. PER2 modulates the clock function by interacting with other circadian regulatory proteins for transporting them to nucleus, and negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. PER2 forms a component of the circadian core oscillator, which also includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS proteinsetc. PER2 interacts with NFIL3, PER1, PER3, CRY1 and CRY2 proteins, and its interaction with CSNK1D or CSNK1E promotes nuclear localization of PER proteins. PER2 is a nuclear protein and its nucleo-cytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. CSNK1E and CSNK1D facilitate PER2 phosphorylation that follows its degradation. PER2 defects have been found to be responsible for familial advanced sleep-phase syndrome (FASPS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PER2 Antibody (NB100-125)(5)

Publications using NB100-125

• Li S, Zhai J, Chu W et al. Altered circadian clock as a novel therapeutic target for constant darkness-induced insulin resistance and hyperandrogenism of polycystic ovary syndrome Transl Res Feb 12 2020 [PMID: 32119846] (WB, Rat)
  
  Details:
  Sprague-Dawley Rats
• Zhang XY, Wang L, Yan WJ et al. Period 2-Induced Activation of Autophagy Improves Cardiac Remodeling After Myocardial Infarction Hum. Gene Ther. Dec 10 2019 [PMID: 31822134] (IHC-P, Mouse)
• Oyama Y, Bartman CM, Bonney S et al. Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the Endothelium Cell Rep Aug 6 2019 [PMID: 31390562] (IP, Human)
• Dong E, Guidotti A, Zhang H et al. Prenatal stress leads to chromatin and synaptic remodeling and excessive alcohol intake comorbid with anxiety-like behaviors in adult offspring Neuropharmacology Sep 15 2018 [PMID: 30016666] (WB, Mouse)
• Lee Y, Jang AR, Francey LJ et al. KPNB1 mediates PER/CRY nuclear translocation and circadian clock function. Elife 2015 [PMID: 26319354] (WB)

Reviews for PER2 Antibody (NB100-125) (3)

Average Rating: 3.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Katharina Sahiri WB Human 02/21/2019

Summary

• Application: Western Blot
• Sample Tested: U-2 OS cells
• Species: Human
• Lot: E

Comments

• Comments: unspecific bands with no obvious band of correct size

reviewed by: Anonymous WB Human 11/12/2017

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell line
• Species: Human
• Lot: D-1

Comments

• Comments: Sample: Whole cell lysates of cancer cell lines (10ug)
  Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
  Primary Antibody diluted (1:500) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
  Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T

reviewed by: Anonymous WB Human 12/10/2014

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysates of various cancer cell lines
• Species: Human
• Lot: C-1

Blocking

• Blocking Details: Blocked in 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature

Primary Anitbody

• Dilution Ratio: Diluted in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG (H&L) HRP-conjugated antibody
• Secondary Manufacturer Cat#: Cell Signaling #7074
• Secondary Concentration: 1:2000

Details

• Detection Notes: Detected using Pierce ECL Western Blotting Substrate, exposing for 1 min, imaged on Amersham LAS 4010

FAQs for PER2 Antibody (NB100-125). (Showing 1 - 1 of 1 FAQs).

1. Would you please let me know if there is any updated data available regarding the testing of this antibody on mouse by WB?
   • We do not have an image showing the detection of NB100-125 in mouse tissues, however we will 100% guarantee this item to work as stated on the datasheet.

Bioinformatics

• Gene Symbol: PER2
• Entrez:
  • 8864(Human)
• Uniprot:
  • O15055(Human)