PER2 Antibody

Images

 
Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PER2 Antibody Summary

Immunogen
Two peptides derived from human PER2 [UniProt# O15055]. Antibodies cross-reacting with each peptide were affinity purified against the appropriate peptide.
Localization
Nuclear
Predicted Species
Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PER2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
Application Notes
This PER2 antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. It has been tested against expressed fragment of human PER2 (bacterially expressed fragment from BL21 cells), or full-length protein expressed in in vitro translation using rabbit reticulocyte lysate. In Western blot, a band can be seen at ~200 kDa representing PER2.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-125 in the following applications:

Publications
Read Publication using NB100-125.

Reactivity Notes

Human. Peptide 1 has 94% homology to rat. Peptide 2 has 94% homology to rat and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for PER2 Antibody

  • Circadian clock protein PERIOD 2
  • FASPS
  • hPER2
  • KIAA0347period 2
  • period (Drosophila) homolog 2
  • period circadian protein 2
  • period circadian protein homolog 2
  • period homolog 2 (Drosophila)

Background

PER2 (period circadian protein homolog 2 or circadian clock protein PERIOD 2) is a component of the circadian clock mechanism which is essential for generating circadian rhythms and PER2 acts as a negative element in the circadian transcriptional loop. PER2 modulates the clock function by interacting with other circadian regulatory proteins for transporting them to nucleus, and negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. PER2 forms a component of the circadian core oscillator, which also includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS proteinsetc. PER2 interacts with NFIL3, PER1, PER3, CRY1 and CRY2 proteins, and its interaction with CSNK1D or CSNK1E promotes nuclear localization of PER proteins. PER2 is a nuclear protein and its nucleo-cytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. CSNK1E and CSNK1D facilitate PER2 phosphorylation that follows its degradation. PER2 defects have been found to be responsible for familial advanced sleep-phase syndrome (FASPS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PER2 Antibody (NB100-125)(1)

We have publications tested in 1 application: WB.


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WB
(1)
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Reviews for PER2 Antibody (NB100-125) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: WB.

Reviews using NB100-125:
Filter by Applications
WB
(2)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot PER2 NB100-125
Enlarge
reviewed by:
Anonymous
WB Human 11/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell line
SpeciesHuman
LotD-1

Comments

CommentsSample: Whole cell lysates of cancer cell lines (10ug)
Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
Primary Antibody diluted (1:500) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
Western Blot PER2 NB100-125
Enlarge
reviewed by:
Anonymous
WB Human 12/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates of various cancer cell lines
SpeciesHuman
LotC-1

Blocking

Blocking DetailsBlocked in 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature

Primary Anitbody

Dilution RatioDiluted in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG (H&L) HRP-conjugated antibody
Secondary Manufacturer Cat#Cell Signaling #7074
Secondary Concentration1:2000

Details

Detection NotesDetected using Pierce ECL Western Blotting Substrate, exposing for 1 min, imaged on Amersham LAS 4010

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PER2 Antibody (NB100-125). (Showing 1 - 1 of 1 FAQ).

  1. Would you please let me know if there is any updated data available regarding the testing of this antibody on mouse by WB?
    • We do not have an image showing the detection of NB100-125 in mouse tissues, however we will 100% guarantee this item to work as stated on the datasheet.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PER2 Antibody (NB100-125)

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Blogs on PER2

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
11/12/2017
Application: WB
Species: Human

Anonymous
12/10/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PER2
Entrez
Uniprot