PER2 Antibody - BSA Free

Images

 
Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.
Immunocytochemistry/ Immunofluorescence: PER2 Antibody [NB100-125] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated ...read more
Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more
Immunocytochemistry/ Immunofluorescence: PER2 Antibody [NB100-125] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

PER2 Antibody - BSA Free Summary

Immunogen
A partial synthetic peptide made to an internal portion of the human PER2 protein (between amino acids 100-150) [UniProt# O15055].
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PER2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200. Use reported in scientific literature (PMID 31822134)
  • Immunoprecipitation reported in scientific literature (PMID 31390562)
  • Western Blot 1 - 2 ug/ml
Application Notes
This PER2 antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. It has been tested against expressed fragment of human PER2 (bacterially expressed fragment from BL21 cells), or full-length protein expressed in in vitro translation using rabbit reticulocyte lysate. In Western blot, a band can be seen at ~200 kDa representing PER2.
Reviewed Applications
Read 3 Reviews rated 3.7
using
NB100-125 in the following applications:

Publications
Read Publications using
NB100-125 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30016666). Use in Rat reported in scientific literature (PMID:32119846).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PER2 Antibody - BSA Free

  • Circadian clock protein PERIOD 2
  • FASPS
  • hPER2
  • KIAA0347period 2
  • period (Drosophila) homolog 2
  • period circadian protein 2
  • period circadian protein homolog 2
  • period homolog 2 (Drosophila)

Background

PER2 (period circadian protein homolog 2 or circadian clock protein PERIOD 2) is a component of the circadian clock mechanism which is essential for generating circadian rhythms and PER2 acts as a negative element in the circadian transcriptional loop. PER2 modulates the clock function by interacting with other circadian regulatory proteins for transporting them to nucleus, and negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. PER2 forms a component of the circadian core oscillator, which also includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS proteinsetc. PER2 interacts with NFIL3, PER1, PER3, CRY1 and CRY2 proteins, and its interaction with CSNK1D or CSNK1E promotes nuclear localization of PER proteins. PER2 is a nuclear protein and its nucleo-cytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. CSNK1E and CSNK1D facilitate PER2 phosphorylation that follows its degradation. PER2 defects have been found to be responsible for familial advanced sleep-phase syndrome (FASPS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PER2 Antibody (NB100-125)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
IP
(1)
WB
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-125 Applications Species
Ding Z, Ge W, Xu X et al. PER2/P65-driven glycogen synthase 1 transcription in macrophages modulates gut inflammation and pathogenesis�of rectal prolapse J Biol Chem 2023-09-01 [PMID: 37660913]
Shearn, CT;Anderson, AL;Devereaux, MW;El Kasmi, KC;Orlicky, DJ;Sokol, RJ; Expression of circadian regulatory genes is dysregulated by increased cytokine production in mice subjected to concomitant intestinal injury and parenteral nutrition PloS one 2023-08-30 [PMID: 37647292] (WB) WB
Fu J, Fan Z, He L et al. Circadian clock disruption in autoimmune thyroiditis European Thyroid Journal 2023-10-01 [PMID: 37548297] (ICC/IF, IHC) ICC/IF, IHC
Oyama y, Shuff SR, Burns N et al. Intense light elicited alveolar type 2 specific circadian PER2 protects from bacterial lung injury via BPIFB1 American journal of physiology. Lung cellular and molecular physiology 2022-03-10 [PMID: 35272486] (WB, Mouse) WB Mouse
Li S, Zhai J, Chu W et al. Altered circadian clock as a novel therapeutic target for constant darkness-induced insulin resistance and hyperandrogenism of polycystic ovary syndrome Transl Res 2020-02-12 [PMID: 32119846] (WB, Rat)

Details:
Sprague-Dawley Rats
WB Rat
Zhang XY, Wang L, Yan WJ et al. Period 2-Induced Activation of Autophagy Improves Cardiac Remodeling After Myocardial Infarction Hum. Gene Ther. 2019-12-10 [PMID: 31822134] (IHC-P, Mouse) IHC-P Mouse
Oyama Y, Bartman CM, Bonney S et al. Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the Endothelium Cell Rep 2019-08-08 [PMID: 31390562] (IP, Human) IP Human
Dong E, Guidotti A, Zhang H et al. Prenatal stress leads to chromatin and synaptic remodeling and excessive alcohol intake comorbid with anxiety-like behaviors in adult offspring Neuropharmacology 2018-09-15 [PMID: 30016666] (WB, Mouse) WB Mouse
Lee Y, Jang AR, Francey LJ et al. KPNB1 mediates PER/CRY nuclear translocation and circadian clock function. Elife 2015-01-01 [PMID: 26319354] (WB) WB

Reviews for PER2 Antibody (NB100-125) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB100-125:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot PER2 NB100-125
Enlarge
2
reviewed by:
Katharina Sahiri
WB Human 02/21/2019
View

Summary

ApplicationWestern Blot
Sample TestedU-2 OS cells
SpeciesHuman
LotE

Comments

Commentsunspecific bands with no obvious band of correct size
Western Blot PER2 NB100-125
Enlarge
5
reviewed by:
Verified Customer
WB Human 11/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell line
SpeciesHuman
LotD-1

Comments

CommentsSample: Whole cell lysates of cancer cell lines (10ug)
Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
Primary Antibody diluted (1:500) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
Western Blot PER2 NB100-125
Enlarge
4
reviewed by:
Verified Customer
WB Human 12/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates of various cancer cell lines
SpeciesHuman
LotC-1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PER2 Antibody (NB100-125). (Showing 1 - 1 of 1 FAQs).

  1. Would you please let me know if there is any updated data available regarding the testing of this antibody on mouse by WB?
    • We do not have an image showing the detection of NB100-125 in mouse tissues, however we will 100% guarantee this item to work as stated on the datasheet.

Secondary Antibodies

 

Isotype Controls

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Array NB100-125

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Research Areas for PER2 Antibody (NB100-125)

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Recent Reviews

3.7
5
1
4
1
3
0
2
1
1
0

Katharina Sahiri
02/21/2019
Application: WB
Species: Human

Verified Customer
11/12/2017
Application: WB
Species: Human

Verified Customer
12/10/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PER2
Entrez
Uniprot