PDK4 Antibody


Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.

Product Details

Reactivity Hu, Mu, Bovine, RatSpecies Glossary
Applications WB, ICC/IF
1.35 mg/ml

PDK4 Antibody Summary

Synthetic peptide made to an internal portion of the human PDK4 protein (within residues 100-150). [Swiss-Prot# Q16654]
Mitochondrion matrix.
Predicted Species
Rat (94%), Bovine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.1% Sodium Azide
1.35 mg/ml
Immunogen affinity purified


  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence
Application Notes
This PDK4 antibody is useful for Western blot, where a band is seen at ~49 kDa. This product is not recommended for ICC, as nonspecific nuclear staining was observed in HeLa cells. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25991547)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
PDK4 Peptide (NBP1-07047PEP)
Reviewed Applications
Read 1 Review rated 3
NBP1-07047 in the following applications:

Read Publications using
NBP1-07047 in the following applications:

Reactivity Notes

Human and Mouse. Predicted to react with bovine based on 100% sequence homology. Immunogen sequence has 94% homology to rat.

Alternate Names for PDK4 Antibody

  • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
  • EC 2.7.11
  • EC
  • FLJ40832
  • Pyruvate dehydrogenase kinase isoform 4
  • pyruvate dehydrogenase kinase, isoenzyme 4
  • pyruvate dehydrogenase kinase, isozyme 4
  • pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial


PDK4 (pyruvate dehydrogenase kinase isoform 4) is expressed ubiquitously in the mitochondrion matrix of tissues with highest expression seen in liver, heart and skeletal muscles. PDK4 is regulated by glucocorticoids, retinoic acid and insulin, and is found elevated with diabetes, fasting and other conditions where fatty acids act as energy source. Under starvation/diabetic conditions, enhanced glucocorticoids levels activate glucocorticoid receptor thereby recruiting p300/CBP coactivator complex to PDK4 gene where it cooperatively interact with FOXO factors and activated RARs to make a stable transcriptional initiation complex responsible for PDK4 activation. Insulin promotes inactivation and translocation of FOXO factors from nucleus to cytoplasm and thereby suppresses glucocorticoid-stimulated PDK4 gene transcription. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex (PDC) via its E1 alpha subunit's phosphorylation, thus contributing to glucose metabolism regulation. Insulin resistance is associated with PDC dysregulation in skeletal muscles and excessive insulin on the other hand downregulates PDK4 expression. PDK4 exerts conservation of glucose as well as three-carbon compounds such as lactate, alanine, pyruvate etc. which serve as substrates for gluconeogenesis and this conservation helps maintain euglycemia during starvation, however, exacerbates hyperglycemia in diabetes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDK4 Antibody (NBP1-07047)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.

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Reviews for PDK4 Antibody (NBP1-07047) (1) 31

Average Rating: 3
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-07047:
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 08/22/2015


ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate


Blocking DetailsBlocking with 4% BSA for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Concentration1:2000


Detection NotesRegular Western blot Protocol

Product General Protocols

View specific protocols for PDK4 Antibody (NBP1-07047): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDK4 Antibody (NBP1-07047). (Showing 1 - 1 of 1 FAQs).

  1. Which PDK4 antibody do you recommend for immunoblot? I'm working with human cell lines.
    • NBP1-07047 and NBP1-07049 are our best selling PDK4 antibodies that will meet your mentioned criteria i.e. Immunoblot/Western Blot in Human samples. However, all of our products are 100% guaranteed and you may find a complete list of PDK4 antibodies by searching PDK4 on our website and narrowing your results using the filters on the left-hand side of the screen.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-07047AF405
Alexa Fluor (R) 488 Labeled NBP1-07047AF488
Alexa Fluor (R) 647 Labeled NBP1-07047AF647
Alexa Fluor (R) 700 Labeled NBP1-07047AF700
Biotin Labeled NBP1-07047B
DyLight 405 Labeled NBP1-07047V
DyLight 488 Labeled NBP1-07047G
DyLight 550 Labeled NBP1-07047R
DyLight 650 Labeled NBP1-07047C
FITC Labeled NBP1-07047F
HRP Labeled NBP1-07047H

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Contact Information

Product PDFs


Gene Symbol PDK4

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