PDK4 Antibody

Images

 
Western Blot: PDK4 Antibody [NBP1-07047] - Mouse liver. NuPAGE 4-12%. This image was submitted via customer review.
Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.35 mg/ml

PDK4 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human PDK4 protein (within residues 100-150). [Swiss-Prot# Q16654]
Localization
Mitochondrion matrix.
Predicted Species
Rat (94%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDK4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence
Application Notes
This PDK4 antibody is useful for Western blot, where a band is seen at ~49 kDa. This product is not recommended for ICC, as nonspecific nuclear staining was observed in HeLa cells. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25991547) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PDK4 Overexpression Lysate
Human Heart Whole Tissue Lysate (Adult Whole Normal)
PDK4 Knockout HeLa Cell Lysate
Control Peptide
Reviewed Applications
Read 2 Reviews rated 3.5
using
NBP1-07047 in the following applications:

Publications
Read Publications using
NBP1-07047 in the following applications:

Reactivity Notes

Human and Mouse. Predicted to react with bovine based on 100% sequence homology. Immunogen sequence has 94% homology to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.35 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDK4 Antibody

  • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
  • EC 2.7.11
  • EC 2.7.11.2
  • FLJ40832
  • Pyruvate dehydrogenase kinase isoform 4
  • pyruvate dehydrogenase kinase, isoenzyme 4
  • pyruvate dehydrogenase kinase, isozyme 4
  • pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial

Background

PDK4 (pyruvate dehydrogenase kinase isoform 4) is expressed ubiquitously in the mitochondrion matrix of tissues with highest expression seen in liver, heart and skeletal muscles. PDK4 is regulated by glucocorticoids, retinoic acid and insulin, and is found elevated with diabetes, fasting and other conditions where fatty acids act as energy source. Under starvation/diabetic conditions, enhanced glucocorticoids levels activate glucocorticoid receptor thereby recruiting p300/CBP coactivator complex to PDK4 gene where it cooperatively interact with FOXO factors and activated RARs to make a stable transcriptional initiation complex responsible for PDK4 activation. Insulin promotes inactivation and translocation of FOXO factors from nucleus to cytoplasm and thereby suppresses glucocorticoid-stimulated PDK4 gene transcription. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex (PDC) via its E1 alpha subunit's phosphorylation, thus contributing to glucose metabolism regulation. Insulin resistance is associated with PDC dysregulation in skeletal muscles and excessive insulin on the other hand downregulates PDK4 expression. PDK4 exerts conservation of glucose as well as three-carbon compounds such as lactate, alanine, pyruvate etc. which serve as substrates for gluconeogenesis and this conservation helps maintain euglycemia during starvation, however, exacerbates hyperglycemia in diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDK4 Antibody (NBP1-07047)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(7)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-07047 Applications Species
Strowitzki MJ, Radhakrishnan P, Pavicevic S et al. High hepatic expression of PDK4 improves survival upon multimodal treatment of colorectal liver metastases Br. J. Cancer Feb 27 2019 [PMID: 30808993] (WB, Human) WB Human
Svensson K, Dent JR, Tahvilian S et al. Defining the contribution of skeletal muscle pyruvate dehydrogenase alpha 1 (Pdha1) to exercise performance and insulin action Am. J. Physiol. Endocrinol. Metab. Aug 28 2018 [PMID: 30153068] (WB, Mouse) WB Mouse
Trinidad AG, Whalley N, Rowlinson R et al. Pyruvate dehydrogenase kinase 4 exhibits a novel role in the activation of mutant KRAS, regulating cell growth in lung and colorectal tumour cells Oncogene 2017 Nov 02 [PMID: 28692044] (WB, Mouse) WB Mouse
Golias T, Papandreou I, Sun R et al. Hypoxic repression of pyruvate dehydrogenase activity is necessary for metabolic reprogramming and growth of model tumours. Sci Rep. Aug 8 2016 [PMID: 27498883] (WB, Human) WB Human
Oikawa S, Mano A, Takahashi R, Kakinuma Y. Remote ischemic preconditioning with a specialized protocol activates the non-neuronal cardiac cholinergic system and increases ATP content in the heart Int. Immunopharmacol. 2015 Jun 11 [PMID: 26072685] (WB, Mouse) WB Mouse
Hou X, Zhang L, Han L et al. Differential function of pyruvate dehydrogenase kinases during mouse oocyte maturation. J. Cell. Sci. 2015 May 19 [PMID: 25991547] (ICC/IF, Mouse) ICC/IF Mouse
Ho N, Coomber BL. Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells. Exp Cell Res 2015 Feb 1 [PMID: 25536473] (Human) Human
Kainulainen H, Papaioannou Kg, Silvennoinen M et al. Myostatin/activin blocking combined with exercise reconditions skeletal muscle expression profile of mdx mice. Mol. Cell. Endocrinol. 2014 Oct 07 [PMID: 25304272] (WB, Mouse) WB Mouse
Rinnankoski-Tuikka R, Silvennoinen M, Torvinen S et al. Effects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle Nutr Metab (Lond) 2012 Jun 9 [PMID: 22682013] (WB, Mouse) WB Mouse
Connaughton S, Chowdhury F, Attia R et al. Regulation of pyruvate dehydrogenase kinase isoform 4 (PDK4) gene expression by glucocorticoids and insulin. Mol Cell Endocrinol. 2010 [PMID: 19703515]

Reviews for PDK4 Antibody (NBP1-07047) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-07047:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PDK4 NBP1-07047
Enlarge
reviewed by:
Thao Luu
WB Mouse 04/05/2018
View

Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesMouse
LotB-4

Comments

CommentsWe used the NuPAGE gel 4-12% for our Westerns.
 
reviewed by:
Anonymous
WB Human 08/22/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 4% BSA for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

View specific protocols for PDK4 Antibody (NBP1-07047): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDK4 Antibody (NBP1-07047). (Showing 1 - 1 of 1 FAQs).

  1. Which PDK4 antibody do you recommend for immunoblot? I'm working with human cell lines.
    • NBP1-07047 and NBP1-07049 are our best selling PDK4 antibodies that will meet your mentioned criteria i.e. Immunoblot/Western Blot in Human samples. However, all of our products are 100% guaranteed and you may find a complete list of PDK4 antibodies by searching PDK4 on our website and narrowing your results using the filters on the left-hand side of the screen.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-07047AF350
Alexa Fluor 405 NBP1-07047AF405
Alexa Fluor 488 NBP1-07047AF488
Alexa Fluor 532 NBP1-07047AF532
Alexa Fluor 594 NBP1-07047AF594
Alexa Fluor 647 NBP1-07047AF647
Alexa Fluor 700 NBP1-07047AF700
Alexa Fluor 750 NBP1-07047AF750
Biotin NBP1-07047B
DyLight 350 NBP1-07047UV
DyLight 405 NBP1-07047V
DyLight 488 NBP1-07047G
DyLight 550 NBP1-07047R
DyLight 594 NBP1-07047DL594
DyLight 650 NBP1-07047C
DyLight 680 NBP1-07047FR
DyLight 755 NBP1-07047IR
FITC NBP1-07047F
HRP NBP1-07047H
Janelia Fluor 549 NBP1-07047JF549
Janelia Fluor 646 NBP1-07047JF646

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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Thao Luu
04/05/2018
Application: WB
Species: Mouse

 
Anonymous
08/22/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol PDK4