BMAL1 Antibody


Western Blot: BMAL1 Antibody [NB100-2288] - Oxidation causes accumulation of BMAL1. Head and neck cancer cells have different expression levels of the core clock protein BMAL1. Image collected and cropped by CiteAb from more
Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse cerebral cortex (left: wt, right: Bmal1 KO) using anti-BMAL1 antibody. Image from verified customer review.
Biological Strategies: Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Inhibition of mTOR signaling with Rapamycin rescues PTEN-driven accumulation of BMAL1A. Tissue samples and graphic show BMAL1 in K14cre more
Biological Strategies: Western Blot: BMAL1 Antibody [NB100-2288] - Oxidation causes accumulation of BMAL1. Western blot assay demonstrates time-dependent accumulation of BMAL1 upon oxidative stress in HNSCC cells more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: BMAL1 Antibody [NB100-2288] - Oxidation causes accumulation of BMAL1. Immunofluorescence assay depicts accumulation of ROS (green channel) and BMAL1 more
Flow (Intracellular): BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on HeLa cells with NB100-2288AF647(blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then more
Genetic Strategies: Knockout Validated: BMAL1 Antibody [NB100-2288] - CamiCre+; BMAL1fx/fx mice are forebrain/SCN-specific BMAL1 knockouts. (J) Immunohistochemistry for BMAL1 on SCN-containing coronal sections of more
Flow Cytometry: BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on Jurkat cells with NB100-2288 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. more
Western Blot: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in A) MCF7, B) NIH/3T3, C) PC12.
Western Blot: BMAL1 Antibody [NB100-2288] - Analysis of MOP3 on 3T3/L1 cell lysates.
Immunocytochemistry/ Immunofluorescence: BMAL1 Antibody [NB100-2288] - BMAL1 antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against more
Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse brain using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: BMAL1 Antibody [NB100-2288] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of rabbit anti-BMAL1 antibody. The staining was developed using HRP labeled anti-rabbit more

Product Details

Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, KO
1.0 mg/ml

Order Details

BMAL1 Antibody Summary

Bacterially expressed human BMAL1 (amino acids 392-626). [UniProt# O00327].
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:250
  • Knockout Validated
  • Western Blot 0.5 ug/mL - 2 ug/mL
Application Notes
This BMAL1 antibody is useful in Immunocytochemistry/Immunofluorescence, Western blot, and IHC-paraffin embedded sections. Use in IHC-frozen sections was reported in the scientific literature (PMID: 23736292). In ICC/IF, primarily nuclear staining was observed with weak cytoplasmic staining in MCF7 cells. In Western Blot, a band was observed ~70 kDa. In IHC-P, staining was observed in the nuclei of mouse brain tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in IHC reported in scientific literature (PMID:33510438)
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
BMAL1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
NB100-2288 in the following applications:

Read Publications using
NB100-2288 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32732906).

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for BMAL1 Antibody

  • aryl hydrocarbon receptor nuclear translocator-like protein 1
  • aryl hydrocarbon receptor nuclear translocator-like
  • basic-helix-loop-helix-PAS orphan MOP3
  • BHLHE5
  • bHLHe5brain and muscle
  • bHLH-PAS protein JAP3
  • BMAL1
  • BMAL1c
  • Brain and muscle ARNT-like 1
  • Class E basic helix-loop-helix protein 5
  • JAP3
  • Member of PAS protein 3
  • member of PAS superfamily 3
  • MOP3
  • MOP3BMAL1c
  • PAS domain-containing protein 3
  • PASD3
  • PASD3MGC47515
  • TIC


BMAL1 (brain and muscle ARNT-like 1; ARNTL) is an essential core component in circadian clock machinery which is regulatory mechanism for circadian rhythms. Circadian clock genes include three period proteins (PER1, PER2 and PER3), two cryptochromes (CRY1 and CRY2), CLOCK, NPAS2 and BMAL proteins, wherein BMAL1 has a potential to heterodimerize with CLOCK or NPAS2 genes; and this neocomplex drives transcription from E-box elements (5'-CACGTG-3') found in circadian-responsive genes's promoters. PER/CRY proteins negatively regulate CLOCK/BMAL1 dimer-mediated transcription, thereby forming the feedback loop that regulates the timing of clock gene transcription. BMAL1 also associates with GNB2L1/RACK1 and PRKCA in a nuclear complex, whertein GNB2L1 and PRKCA are recruited to the complex in a circadian manner. BMAL1 undergoes acetylation (Lys-538), phosphorylation and sumoylation (Lys-259) upon dimerization with CLOCK and acetylation facilitates CRY1-mediated repression. CLOCK-BMAL1 double mutations within PAS domains leads to syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity. Clock genes functions primarily as tumor suppressors and their abbarant expression is observed in malignant pleural mesothelioma, colorectal and breast cancer.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BMAL1 Antibody (NB100-2288)(34)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NB100-2288 Applications Species
de Zavalia N, Schoettner K, Goldsmith JA Et al. Bmal1 in the striatum influences alcohol intake in a sexually dimorphic manner Communications biology Oct 26 2021 [PMID: 34702951] (WB, Mouse) WB Mouse
Gegnaw ST, Sandu C, Mendoza J Et al. Dark-adapted light response in mice is regulated by a circadian clock located in rod photoreceptors Experimental eye research Oct 22 2021 [PMID: 34695438] (IHC, Mouse) IHC Mouse
Trebucq L, Cardama G, Lorenzano Menna P Et Al. Timing of Novel Drug 1A-116 to Circadian Rhythms Improves Therapeutic Effects against Glioblastoma Pharmaceutics Jul 16 2021 [PMID: 34371781] (WB) WB
Huang W, Zheng X, Yang M et al. PER2-mediated ameloblast differentiation via PPAR gamma/AKT1/beta-catenin axis International journal of oral science May 19 2021 [PMID: 34011974]
Kim H, Wei J, Song Z et al. Regulation of Hepatic Circadian Metabolism by the E3 ubiquitin ligase HRD1-controlled CREBH/PPARalpha Transcriptional Program Molecular metabolism Feb 13 2021 [PMID: 33592335] (WB, Mouse) WB Mouse
Kim B, Kim J, Chun M, et al. Multiplexed CRISPR-Cas9 system in a single adeno-associated virus to simultaneously knock out redundant clock genes Scientific reports Jan 28 2021 [PMID: 33510438] (WB, IHC, Mouse) WB, IHC Mouse
Wang XL, Wolff SEC, Korpel N et al. Deficiency of the Circadian Clock Gene Bmal1 Reduces Microglial Immunometabolism Frontiers in immunology Dec 8 2020 [PMID: 33363534] (WB, Mouse) WB Mouse
Greco CM, Cervantes M, Fustin JM et al. S-adenosyl-l-homocysteine hydrolase links methionine metabolism to the circadian clock and chromatin remodeling Science advances Dec 1 2020 [PMID: 33328229] (ICC/IF, Mouse) ICC/IF Mouse
Sominsky L, Dangel T, Malik S et al. Microglial ablation in rats disrupts the circadian system Nat Commun Nov 13 2020 [PMID: 33200466] (ICC/IF, WB, Human) ICC/IF, WB Human
Kim ER, Xu Y, Cassidy RM et al. Paraventricular hypothalamus mediates diurnal rhythm of metabolism Nat Commun Jul 30 2020 [PMID: 32732906] (IHC, Mouse) IHC Mouse
Show All 34 Publications.

Review for BMAL1 Antibody (NB100-2288) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2288:
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Images Ratings Applications Species Date Details
Immunofluorescence BMAL1 NB100-2288
reviewed by:
Verified Customer
IF Mouse 06/29/2015


Sample Testedmouse brain


Blocking Details5% goat serum in TBS+0.05% Triton X-100 x 30min.

Primary Anitbody

Dilution RatioNB100-2288 1:1000 in TBS+0.05% Triton X-100 + 1% goat serum. Incubated overnight at 4C.

Secondary Antibody

Secondary DescriptionAlexaflor 568-conjugated Goat anti-rabbit, 1 hr incubation at room temp, in TBS+0.05% Triton X-10 . Then washed 3 x 5min in TBS


Detection NotesNikon fluorescent microscope, 568nm. Bmal1 KO brain tissue used as negative control (shown)
Fixation DetailsMouse perfused with PBS+heparin, brain submerged in 3% paraformaldehyde x 24hrs, then 30% sucrose at -20C x 48 hrs.
Wash DescriptionTBS: 3 x 5min washes


CommentsExcellent antibody for IHC. Does not stain Bmal1 KO tissue (as many other Bmal1 antibodies do).

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for BMAL1 Antibody (NB100-2288) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2288AF350
Alexa Fluor 405 NB100-2288AF405
Alexa Fluor 488 NB100-2288AF488
Alexa Fluor 532 NB100-2288AF532
Alexa Fluor 594 NB100-2288AF594
Alexa Fluor 647 NB100-2288AF647
Alexa Fluor 700 NB100-2288AF700
Alexa Fluor 750 NB100-2288AF750
Allophycocyanin NB100-2288APC
Biotin NB100-2288B
DyLight 350 NB100-2288UV
DyLight 405 NB100-2288V
DyLight 488 NB100-2288G
DyLight 550 NB100-2288R
DyLight 594 NB100-2288DL594
DyLight 650 NB100-2288C
DyLight 680 NB100-2288FR
DyLight 755 NB100-2288IR
FITC NB100-2288F
HRP NB100-2288H
Janelia Fluor 549 NB100-2288JF549
Janelia Fluor 646 NB100-2288JF646
PE NB100-2288PE
PerCP NB100-2288PCP

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Recent Reviews


Verified Customer
Application: IF
Species: Mouse


Gene Symbol ARNTL