BMAL1 Antibody

Images

 
Western Blot: BMAL1 Antibody [NB100-2288] - Oxidation causes accumulation of BMAL1. Head and neck cancer cells have different expression levels of the core clock protein BMAL1. Image collected and cropped by CiteAb from ...read more
Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse cerebral cortex (left: wt, right: Bmal1 KO) using anti-BMAL1 antibody. Image from verified customer review.
Biological Strategies: Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Inhibition of mTOR signaling with Rapamycin rescues PTEN-driven accumulation of BMAL1A. Tissue samples and graphic show BMAL1 in K14cre ...read more
Biological Strategies: Western Blot: BMAL1 Antibody [NB100-2288] - Oxidation causes accumulation of BMAL1. Western blot assay demonstrates time-dependent accumulation of BMAL1 upon oxidative stress in HNSCC cells ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: BMAL1 Antibody [NB100-2288] - Oxidation causes accumulation of BMAL1. Immunofluorescence assay depicts accumulation of ROS (green channel) and BMAL1 ...read more
Flow (Intracellular): BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on HeLa cells with NB100-2288AF647(blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Genetic Strategies: Knockout Validated: BMAL1 Antibody [NB100-2288] - CamiCre+; BMAL1fx/fx mice are forebrain/SCN-specific BMAL1 knockouts. (J) Immunohistochemistry for BMAL1 on SCN-containing coronal sections of ...read more
Flow Cytometry: BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on Jurkat cells with NB100-2288 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. ...read more
Western Blot: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in A) MCF7, B) NIH/3T3, C) PC12.
Western Blot: BMAL1 Antibody [NB100-2288] - Analysis of MOP3 on 3T3/L1 cell lysates.
Immunocytochemistry/ Immunofluorescence: BMAL1 Antibody [NB100-2288] - BMAL1 antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against ...read more
Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse brain using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: BMAL1 Antibody [NB100-2288] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of rabbit anti-BMAL1 antibody. The staining was developed using HRP labeled anti-rabbit ...read more
Immunohistochemistry-Frozen: Rabbit Polyclonal BMAL1 Antibody [NB100-2288] - IHC-fixed frozen in the brain of green tree frogs. DAB staining, 1:1000 dilution. Image from a verified customer review.
CamiCre+; Bmal1fx/fx mice are forebrain/SCN-specific Bmal1 knockouts. (J) Immunohistochemistry for BMAL1 on SCN-containing coronal sections of Fx/Fx (n = 3), BKO (n = 3), and Bmal1-/- (KO, n = 1) mice sacrificed at ...read more
Astrocyte Bmal1 regulates genes with conflicting effects on Abeta deposition. (A) Topro, GFAP, and BMAL1 staining in CA1 hippocampus of 4-month-old BMAL1 aKO; APP/PS1-21 mice and Cre- controls (scale bar = 100 um). ...read more
Upregulation of HIF-1 alpha in the spleen restricts CD8+ T cell expansion.(A-B) Mice were infected with 2x107 amastigotes intravenously and euthanized at various time points after infection. HIF-1 alpha expression in ...read more
Hypoxia reduces inflammatory signaling pathways and NLRP3 expression and induces autophagy in IECs. a HT-29 cells were subjected to normoxia and hypoxia at the indicated times in the absence or presence of 10 µg/ml ...read more
Expression of necroptosis- and apoptosis-related markers is elevated in OA cartilage.Necroptosis- and apoptosis-related markers in highly degenerated cartilage tissue of OA patients (ICRS grade ≥ 3) were ...read more
Osteogenic differentiation in 3D. (A) Histochemical staining of 12-μm-thick gel sections from one representative donor after 5 weeks of culture in undifferentiated or osteogenic conditions. Osteoimage® ...read more
Rac1 inhibition abolishes IR-induced activation of both ATM and ATR signaling. (A) MCF-7 cells were treated with/without 20-Gy IR in the presence or absence of 100 μM NSC23766 and incubated for 1 hour at 37°C before ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, ChIP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

BMAL1 Antibody Summary

Immunogen
Bacterially expressed human BMAL1 (amino acids 392-626). [UniProt# O00327].
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARNTL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:250. Use reported in scientific literature (PMID 33510438)
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 23736292)
  • Immunohistochemistry-Paraffin 1:250
  • Knockout Validated
  • Western Blot 0.5 ug/mL - 2 ug/mL
Application Notes
In ICC/IF, primarily nuclear staining was observed with weak cytoplasmic staining in MCF7 cells. In Western Blot, a band was observed ~70 kDa. In IHC-P, staining was observed in the nuclei of mouse brain tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
BMAL1 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-2288 in the following applications:

Publications
Read Publications using
NB100-2288 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32732906). Amphibian reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BMAL1 Antibody

  • ARNTL
  • aryl hydrocarbon receptor nuclear translocator-like protein 1
  • aryl hydrocarbon receptor nuclear translocator-like
  • basic-helix-loop-helix-PAS orphan MOP3
  • BHLHE5
  • bHLHe5brain and muscle
  • bHLH-PAS protein JAP3
  • BMAL1
  • BMAL1c
  • BMAL1TIC
  • Brain and muscle ARNT-like 1
  • Class E basic helix-loop-helix protein 5
  • JAP3
  • Member of PAS protein 3
  • member of PAS superfamily 3
  • MOP3
  • MOP3BMAL1c
  • PAS domain-containing protein 3
  • PASD3
  • PASD3MGC47515
  • TIC

Background

BMAL1 (brain and muscle ARNT-like 1; ARNTL) is an essential core component in circadian clock machinery which is regulatory mechanism for circadian rhythms. Circadian clock genes include three period proteins (PER1, PER2 and PER3), two cryptochromes (CRY1 and CRY2), CLOCK, NPAS2 and BMAL proteins, wherein BMAL1 has a potential to heterodimerize with CLOCK or NPAS2 genes; and this neocomplex drives transcription from E-box elements (5'-CACGTG-3') found in circadian-responsive genes's promoters. PER/CRY proteins negatively regulate CLOCK/BMAL1 dimer-mediated transcription, thereby forming the feedback loop that regulates the timing of clock gene transcription. BMAL1 also associates with GNB2L1/RACK1 and PRKCA in a nuclear complex, whertein GNB2L1 and PRKCA are recruited to the complex in a circadian manner. BMAL1 undergoes acetylation (Lys-538), phosphorylation and sumoylation (Lys-259) upon dimerization with CLOCK and acetylation facilitates CRY1-mediated repression. CLOCK-BMAL1 double mutations within PAS domains leads to syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity. Clock genes functions primarily as tumor suppressors and their abbarant expression is observed in malignant pleural mesothelioma, colorectal and breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMAL1 Antibody (NB100-2288)(65)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 10 applications: B/N, Chemotaxis, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, KD, WB.


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B/N
(1)
Chemotaxis
(4)
FLOW
(1)
ICC/IF
(14)
IF/IHC
(8)
IHC
(10)
IHC-Fr
(3)
IHC-P
(5)
KD
(1)
WB
(20)
All Applications
Filter By Species
Human
(8)
Mouse
(32)
Rat
(3)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using NB100-2288 Applications Species
Shearn, CT;Anderson, AL;Devereaux, MW;El Kasmi, KC;Orlicky, DJ;Sokol, RJ; Expression of circadian regulatory genes is dysregulated by increased cytokine production in mice subjected to concomitant intestinal injury and parenteral nutrition PloS one 2023-08-30 [PMID: 37647292] (WB) WB
Halawani, D;Wang, Y;Ramakrishnan, A;Estill, M;He, X;Shen, L;Friedel, RH;Zou, H; Circadian clock regulator Bmal1 gates axon regeneration via Tet3 epigenetics in mouse sensory neurons Nature communications 2023-08-24 [PMID: 37620297]
Fu J, Fan Z, He L et al. Circadian clock disruption in autoimmune thyroiditis European Thyroid Journal 2023-10-01 [PMID: 37548297] (IHC, ICC/IF) IHC, ICC/IF
Gobbo F, Zingariello M, Verachi P et al. GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosis bioRxiv 2023-09-19 [PMID: 37425686]
Kinane C, Calligaro H, Jandot A et al. Dopamine modulates the retinal clock through melanopsin-dependent regulation of cholinergic waves during development BMC biology 2023-06-26 [PMID: 37365544] (IHC-Fr, Mouse) IHC-Fr Mouse
Sheehan PW, Nadarajah CJ, Kanan MF et al. An astrocyte BMAL1-BAG3 axis protects against alpha-synuclein and tau pathology Neuron 2023-06-06 [PMID: 37315555] (IHC, WB, KD, Mouse) IHC, WB, KD Mouse
Schwartz PB, Nukaya M, Berres ME et al. The circadian clock is disrupted in pancreatic cancer PLOS Genetics 2023-06-01 [PMID: 37262074] (WB) WB
Mendoza-Viveros L, Marmolejo-Gutierrez C, Cid-Castro C et al. Astrocytic circadian clock control of energy expenditure by transcriptional stress responses in the ventromedial hypothalamus Glia 2023-03-15 [PMID: 36919670]
Schwartz PB, Walcheck MT, Nukaya M et al. Chronic jetlag accelerates pancreatic neoplasia in conditional Kras-mutant mice Chronobiology international 2023-04-01 [PMID: 36912021] (Mouse) Mouse
Gonzalez JC, Lee H, Vincent AM et al. Circadian regulation of dentate gyrus excitability mediated by G-protein signaling Cell reports 2023-02-06 [PMID: 36749664] (IHC, Mouse) IHC Mouse
Show All 65 Publications.

Reviews for BMAL1 Antibody (NB100-2288) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Hyla cinerea.

Reviews using NB100-2288:
Filter by Applications
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(1)
Hyla cinerea
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen BMAL1 NB100-2288
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3
reviewed by:
Deborah Lutterschmidt
IHC-Fr Hyla cinerea 10/20/2023
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain
SpeciesHyla cinerea
LotD146902

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
Immunofluorescence BMAL1 NB100-2288
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5
reviewed by:
Verified Customer
IF Mouse 06/29/2015
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Summary

ApplicationImmunofluorescence
Sample Testedmouse brain
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BMAL1 Antibody (NB100-2288) (0)

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Recent Reviews

4
5
1
4
0
3
1
2
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1
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Deborah Lutterschmidt
10/20/2023
Application: IHC-Fr
Species: Hyla cinerea

Verified Customer
06/29/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol ARNTL