BMAL1 Antibody

Images

 
Western Blot: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in A) MCF7, B) NIH/3T3, C) PC12.
Immunocytochemistry/ Immunofluorescence: BMAL1 Antibody [NB100-2288] - BMAL1 antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against ...read more
Immunohistochemistry-Paraffin: BMAL1 Antibody [NB100-2288] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of rabbit anti-BMAL1 antibody. The staining was developed using HRP labeled anti-rabbit ...read more
Western Blot: BMAL1 Antibody [NB100-2288] - Analysis of MOP3 on 3T3/L1 cell lysates.
Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse brain using DAB with hematoxylin counterstain.
Flow Cytometry: BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on Jurkat cells with BMAL1 Antibody NB100-2288 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with ...read more
Chromatin Immunoprecipitation: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse cerebral cortex (left: wt, right: Bmal1 KO) using anti-BMAL1 antibody. Image from verified customer review.
Flow (Intracellular): BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on HeLa cells with BMAL1 Antibody NB100-2288AF647(blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

BMAL1 Antibody Summary

Immunogen
Bacterially expressed human BMAL1 (C-terminus). [UniProt# O00327].
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARNTL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL - 2 ug/mL
  • Chromatin Immunoprecipitation
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:250
  • Flow (Intracellular)
Application Notes
This BMAL1 antibody is useful in Immunocytochemistry/Immunofluorescence, Western blot, and IHC-paraffin embedded sections. Use in IHC-frozen sections was reported in the scientific literature (PMID: 23736292). In ICC/IF, primarily nuclear staining was observed with weak cytoplasmic staining in MCF7 cells. In Western Blot, a band was observed ~70 kDa. In IHC-P, staining was observed in the nuclei of mouse brain tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
BMAL1 Overexpression Lysate
BMAL1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-2288 in the following applications:

Publications
Read Publications using
NB100-2288 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Glycine
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BMAL1 Antibody

  • aryl hydrocarbon receptor nuclear translocator-like protein 1
  • aryl hydrocarbon receptor nuclear translocator-like
  • basic-helix-loop-helix-PAS orphan MOP3
  • BHLHE5
  • bHLHe5brain and muscle
  • bHLH-PAS protein JAP3
  • BMAL1TIC
  • Brain and muscle ARNT-like 1
  • Class E basic helix-loop-helix protein 5
  • Member of PAS protein 3
  • member of PAS superfamily 3
  • MOP3BMAL1c
  • PAS domain-containing protein 3
  • PASD3MGC47515

Background

BMAL1 (brain and muscle ARNT-like 1; ARNTL) is an essential core component in circadian clock machinery which is regulatory mechanism for circadian rhythms. Circadian clock genes include three period proteins (PER1, PER2 and PER3), two cryptochromes (CRY1 and CRY2), CLOCK, NPAS2 and BMAL proteins, wherein BMAL1 has a potential to heterodimerize with CLOCK or NPAS2 genes; and this neocomplex drives transcription from E-box elements (5'-CACGTG-3') found in circadian-responsive genes's promoters. PER/CRY proteins negatively regulate CLOCK/BMAL1 dimer-mediated transcription, thereby forming the feedback loop that regulates the timing of clock gene transcription. BMAL1 also associates with GNB2L1/RACK1 and PRKCA in a nuclear complex, whertein GNB2L1 and PRKCA are recruited to the complex in a circadian manner. BMAL1 undergoes acetylation (Lys-538), phosphorylation and sumoylation (Lys-259) upon dimerization with CLOCK and acetylation facilitates CRY1-mediated repression. CLOCK-BMAL1 double mutations within PAS domains leads to syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity. Clock genes functions primarily as tumor suppressors and their abbarant expression is observed in malignant pleural mesothelioma, colorectal and breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2288
Species: Hu, Mu, Rt, Pm
Applications: WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for BMAL1 Antibody (NB100-2288)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ChIP
(2)
ICC/IF
(5)
IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
WB
(4)
All Applications
Filter By Species
Human
(4)
Mouse
(9)
Rat
(2)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-2288 Applications Species
Hannibal J, Norn THB, Georg B et al. Spatiotemporal expression pattern of PERIOD1 and PERIOD 2 in the mouse SCN is dependent on VIP receptor2 signaling Eur. J. Neurosci. Jun 18 2019 [PMID: 31211910]
Luo Q, Xiao Y, Alex A et al. The Diurnal Rhythm of Insulin Receptor Substrate-1 (IRS-1) and Kir4.1 in Diabetes: Implications for a Clock Gene Bmal1 Invest. Ophthalmol. Vis. Sci. May 1 2019 [PMID: 31042800] (WB, Human, Rat) WB Human, Rat
Cedernaes J, Huang W, Ramsey KM et al. Transcriptional Basis for Rhythmic Control of Hunger and Metabolism within the AgRP Neuron Cell Metab. Feb 18 2019 [PMID: 30827863] (IHC, Mouse) IHC Mouse
Zhu M, Tang H, Tang X et al. BMAL1 suppresses ROS-induced endothelial-to-mesenchymal transition and atherosclerosis plaque progression via BMP signaling. Am J Transl Res. Oct 15 2018 [PMID: 30416657] (WB, Human) WB Human
Das V, Kc R, Li X, Varma D. Pharmacological targeting of the mammalian clock reveals a novel analgesic for osteoarthritis-induced pain. Gene. 2018 Feb 20 [PMID: 29474860] (Mouse) Mouse
Kress GJ, Liao F, Dimitry J et al. Regulation of amyloid-B dynamics and pathology by the circadian clock. J. Exp. Med. Apr 2 2018 [PMID: 29382695] (ICC/IF, Mouse) ICC/IF Mouse
Bostrom J, Sramkova Z, Salasova A et al. Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells. PLoS ONE 2017 Dec 11 [PMID: 29228002] (ICC/IF, Human) ICC/IF Human
Lai B, Zou J, Lin Z et al. Haploinsufficiency of hnRNP U Changes Activity Pattern and Metabolic Rhythms Am. J. Pathol. 2017 Nov 09 [PMID: 29128567] (Mouse) Mouse
Frederick A, Goldsmith J, de Zavalia N, Amir S. Mapping the co-localization of the circadian proteins PER2 and BMAL1 with enkephalin and substance P throughout the rodent forebrain. PLoS ONE. Apr 19 2017 [PMID: 28423013] (ICC/IF, Rat) ICC/IF Rat
Dumbell R, Leliavski A, Matveeva O et al. Dissociation of Molecular and Endocrine Circadian Rhythms in Male Mice Lacking Bmal1 in the Adrenal Cortex. Endocrinology 2016 Nov [PMID: 27690690] (Mouse) Mouse
Show All 16 Publications.

Review for BMAL1 Antibody (NB100-2288) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2288:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence BMAL1 NB100-2288
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reviewed by:
Anonymous
IF Mouse 06/29/2015
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Summary

ApplicationImmunofluorescence
Sample Testedmouse brain
SpeciesMouse

Blocking

Blocking Details5% goat serum in TBS+0.05% Triton X-100 x 30min.

Primary Anitbody

Dilution RatioNB100-2288 1:1000 in TBS+0.05% Triton X-100 + 1% goat serum. Incubated overnight at 4C.

Secondary Antibody

Secondary DescriptionAlexaflor 568-conjugated Goat anti-rabbit, 1 hr incubation at room temp, in TBS+0.05% Triton X-10. Then washed 3 x 5min in TBS

Details

Detection NotesNikon fluorescent microscope, 568nm. Bmal1 KO brain tissue used as negative control (shown)
Fixation DetailsMouse perfused with PBS+heparin, brain submerged in 3% paraformaldehyde x 24hrs, then 30% sucrose at -20C x 48 hrs.
Wash DescriptionTBS: 3 x 5min washes

Comments

CommentsExcellent antibody for IHC. Does not stain Bmal1 KO tissue (as many other Bmal1 antibodies do).

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for BMAL1 Antibody (NB100-2288) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2288AF350
Alexa Fluor 405 NB100-2288AF405
Alexa Fluor 488 NB100-2288AF488
Alexa Fluor 532 NB100-2288AF532
Alexa Fluor 594 NB100-2288AF594
Alexa Fluor 647 NB100-2288AF647
Alexa Fluor 700 NB100-2288AF700
Alexa Fluor 750 NB100-2288AF750
Allophycocyanin NB100-2288APC
Biotin NB100-2288B
DyLight 350 NB100-2288UV
DyLight 405 NB100-2288V
DyLight 488 NB100-2288G
DyLight 550 NB100-2288R
DyLight 594 NB100-2288DL594
DyLight 650 NB100-2288C
DyLight 680 NB100-2288FR
DyLight 755 NB100-2288IR
FITC NB100-2288F
HRP NB100-2288H
Janelia Fluor 549 NB100-2288JF549
Janelia Fluor 646 NB100-2288JF646
PE NB100-2288PE
PerCP NB100-2288PCP

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/29/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol ARNTL