Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
Applications | WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, KO |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Concentration | 1.0 mg/ml |
Immunogen | Bacterially expressed human BMAL1 (amino acids 392-626). [UniProt# O00327]. |
Localization | Nuclear |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | ARNTL |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | This BMAL1 antibody is useful in Immunocytochemistry/Immunofluorescence, Western blot, and IHC-paraffin embedded sections. Use in IHC-frozen sections was reported in the scientific literature (PMID: 23736292). In ICC/IF, primarily nuclear staining was observed with weak cytoplasmic staining in MCF7 cells. In Western Blot, a band was observed ~70 kDa. In IHC-P, staining was observed in the nuclei of mouse brain tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in IHC reported in scientific literature (PMID:33510438) |
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Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at 4C. Do not freeze. |
Buffer | Tris-Glycine |
Preservative | 0.02% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Erik Musiek |
IF | Mouse | 06/29/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
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Gene Symbol | ARNTL |