BMAL1 Antibody

Images

 
Western Blot: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in A) MCF7, B) NIH/3T3, C) PC12.
Immunocytochemistry/ Immunofluorescence: BMAL1 Antibody [NB100-2288] - BMAL1 antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against ...read more
Immunohistochemistry-Paraffin: BMAL1 Antibody [NB100-2288] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using 1:200 dilution of rabbit anti-BMAL1 antibody. The staining was ...read more
Western Blot: BMAL1 Antibody [NB100-2288] - Analysis of MOP3 on 3T3/L1 cell lysates.
Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse brain using DAB with hematoxylin counterstain.
Immunofluorescence: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse cerebral cortex (left: wt, right: Bmal1 KO) using anti-BMAL1 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

BMAL1 Antibody Summary

Immunogen
Bacterially expressed human BMAL1 (C-terminus). [UniProt# O00327].
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARNTL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100 - 1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:250
Application Notes
This BMAL1 antibody is useful in Immunocytochemistry/Immunofluorescence, Western blot, and IHC-paraffin embedded sections. Use in IHC-frozen sections was reported in the scientific literature (PMID: 23736292). In ICC/IF, primarily nuclear staining was observed with weak cytoplasmic staining in MCF7 cells. In Western Blot, a band was observed ~70 kDa. In IHC-P, staining was observed in the nuclei of mouse brain tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
BMAL1 Lysate (NBL1-07722)
Reviewed Applications
Read 1 Review rated 5
using
NB100-2288 in the following applications:

Publications
Read Publications using
NB100-2288 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BMAL1 Antibody

  • aryl hydrocarbon receptor nuclear translocator-like protein 1
  • aryl hydrocarbon receptor nuclear translocator-like
  • basic-helix-loop-helix-PAS orphan MOP3
  • BHLHE5
  • bHLHe5brain and muscle
  • bHLH-PAS protein JAP3
  • BMAL1TIC
  • Brain and muscle ARNT-like 1
  • Class E basic helix-loop-helix protein 5
  • Member of PAS protein 3
  • member of PAS superfamily 3
  • MOP3BMAL1c
  • PAS domain-containing protein 3
  • PASD3MGC47515

Background

BMAL1 (brain and muscle ARNT-like 1; ARNTL) is an essential core component in circadian clock machinery which is regulatory mechanism for circadian rhythms. Circadian clock genes include three period proteins (PER1, PER2 and PER3), two cryptochromes (CRY1 and CRY2), CLOCK, NPAS2 and BMAL proteins, wherein BMAL1 has a potential to heterodimerize with CLOCK or NPAS2 genes; and this neocomplex drives transcription from E-box elements (5'-CACGTG-3') found in circadian-responsive genes's promoters. PER/CRY proteins negatively regulate CLOCK/BMAL1 dimer-mediated transcription, thereby forming the feedback loop that regulates the timing of clock gene transcription. BMAL1 also associates with GNB2L1/RACK1 and PRKCA in a nuclear complex, whertein GNB2L1 and PRKCA are recruited to the complex in a circadian manner. BMAL1 undergoes acetylation (Lys-538), phosphorylation and sumoylation (Lys-259) upon dimerization with CLOCK and acetylation facilitates CRY1-mediated repression. CLOCK-BMAL1 double mutations within PAS domains leads to syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity. Clock genes functions primarily as tumor suppressors and their abbarant expression is observed in malignant pleural mesothelioma, colorectal and breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2288
Species: Hu, Mu, Rt, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for BMAL1 Antibody (NB100-2288)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC-Fr, IHC-P, WB.


Filter By Application
ChIP
(2)
ICC/IF
(4)
IHC-Fr
(1)
IHC-P
(2)
WB
(2)
All Applications
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Human
(1)
Mouse
(5)
Rat
(2)
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Showing Publications 1 - 9 of 9.
Publications using NB100-2288 Applications Species
Frederick A, Goldsmith J, de Zavalia N, Amir S. Mapping the co-localization of the circadian proteins PER2 and BMAL1 with enkephalin and substance P throughout the rodent forebrain. PLoS ONE. Apr 19 2017 [PMID: 28423013] (ICC/IF, Rat) ICC/IF Rat
Dumbell R, Leliavski A, Matveeva O et al. Dissociation of Molecular and Endocrine Circadian Rhythms in Male Mice Lacking Bmal1 in the Adrenal Cortex. Endocrinology 2016 Nov [PMID: 27690690] (Mouse) Mouse
Matsumoto CS, Almeida LO, Guimaraes DM et al. PI3K-PTEN dysregulation leads to mTOR-driven upregulation of the core clock gene BMAL1 in normal and malignant epithelial cells. Oncotarget. Jul 5 2016 [PMID: 27285754] (ICC/IF, WB, Human) ICC/IF, WB Human
Perelis M, Marcheva B, Ramsey KM et al. Pancreatic B cell enhancers regulate rhythmic transcription of genes controlling insulin secretion. Science. 2015 Nov 06 [PMID: 26542580] (IHC-P, Mouse) IHC-P Mouse
Leliavski A, Shostak A, Husse J, Oster H. Impaired glucocorticoid production and response to stress in Arntl-deficient male mice. Endocrinology. 2014 Jan 01 [PMID: 24189141] (IHC-P) IHC-P
Obi-Ioka Y, Ushijima K, Kusama M et al. Involvement of Wee1 in the circadian rhythm dependent intestinal damage induced by docetaxel. J Pharmacol Exp Ther 2013 Jul 26 [PMID: 23892568] (ChIP, Mouse) ChIP Mouse
Chu A, Zhu L, Blum ID et al. Global but not gonadotrope-specific disruption of bmal1 abolishes the luteinizing hormone surge without affecting ovulation. Endocrinology 2013 Aug [PMID: 23736292] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Lauzier A, Charbonneau M, Harper K et al. Formation of invadopodia-like structures by synovial cells promotes cartilage breakdown in collagen-induced arthritis: Involvement of the protein tyrosine kinase Src. Arthritis Rheum;63(6):1591-1602. 2011 Jun. [PMID: 21337539] (ICC/IF, Rat) ICC/IF Rat
Hayashida S, Kuramoto Y, Koyanagi S et al. Proxisome proliferator-activated receptor-alpha mediates high-fat, diet-enhanced daily oscillation of plasminogen activator inhibitor-1 activity in mice. Chronobiol Int 2010 Oct [PMID: 20969520] (WB, ChIP, Mouse) WB, ChIP Mouse

Review for BMAL1 Antibody (NB100-2288) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IF.

Reviews using NB100-2288:
Filter by Applications
IF
(1)
All Applications
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Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence BMAL1 NB100-2288
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reviewed by:
Anonymous
IF Mouse 06/29/2015
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Summary

ApplicationImmunofluorescence
Sample Testedmouse brain
SpeciesMouse

Blocking

Blocking Details5% goat serum in TBS+0.05% Triton X-100 x 30min.

Primary Anitbody

Dilution RatioNB100-2288 1:1000 in TBS+0.05% Triton X-100 + 1% goat serum. Incubated overnight at 4C.

Secondary Antibody

Secondary DescriptionAlexaflor 568-conjugated Goat anti-rabbit, 1 hr incubation at room temp, in TBS+0.05% Triton X-10. Then washed 3 x 5min in TBS

Details

Detection NotesNikon fluorescent microscope, 568nm. Bmal1 KO brain tissue used as negative control (shown)
Fixation DetailsMouse perfused with PBS+heparin, brain submerged in 3% paraformaldehyde x 24hrs, then 30% sucrose at -20C x 48 hrs.
Wash DescriptionTBS: 3 x 5min washes

Comments

CommentsExcellent antibody for IHC. Does not stain Bmal1 KO tissue (as many other Bmal1 antibodies do).

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for BMAL1 Antibody (NB100-2288) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2288AF405
Alexa Fluor 488 Labeled NB100-2288AF488
Alexa Fluor 647 Labeled NB100-2288AF647
Alexa Fluor 700 Labeled NB100-2288AF700
Biotin Labeled NB100-2288B
DyLight 405 Labeled NB100-2288V
DyLight 488 Labeled NB100-2288G
DyLight 550 Labeled NB100-2288R
DyLight 650 Labeled NB100-2288C
FITC Labeled NB100-2288F
HRP Labeled NB100-2288H

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/29/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol ARNTL