Glut4 Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Glut4 Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human Glucose Transporter GLUT4 protein (between residues 480-509) [UniProt P14672]
Localization
Endomembrane system; Multi-pass membrane protein. Cytoplasm - perinuclear region. Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A4
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This GLUT4 antibody is useful for Western blot, IHC and ICC/IF. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24339864). Flow cytometry data from customer review.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-49533C in the following applications:

Reactivity Notes

Human and mouse. Predicted to react with rat based on 100% sequence homology. Rat reactivity reported in scientific literature (PMID: 26468734)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Glut4 Antibody [DyLight 650]

  • Glut4
  • GLUT-4
  • GLUT4Glucose transporter type 4, insulin-responsive
  • insulin-responsive glucose transporter type 4
  • SLC2A4
  • solute carrier family 2 (facilitated glucose transporter), member 4
  • solute carrier family 2, facilitated glucose transporter member 4

Background

Glucose Transporter 4 (GLUT4/SLC2A4) is a member of a family of proteins that facilitate glucose transport. In response to insulin GLUT4 translocates from low density microsomes to the plasma membrane. Activation of phosphatidylinositol-3-OH kinase is required for this trafficking event but is not sufficient. Insulin resistant subjects with and without diabetes show abnormal accumulation of GLUT4 in a dense membrane compartment in muscle and adipocytes from which insulin is unable to recruit GLUT4 to the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut4 Antibody (NBP1-49533C) (0)

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Review for Glut4 Antibody (NBP1-49533C) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-49533C:
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reviewed by:
Brad Poteat
Flow Mouse 04/02/2014
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Summary

ApplicationFlow Cytometry
Sample TestedMouse bone marrow mononuclear cells
SpeciesMouse
LotA-3

Primary Anitbody

Dilution Ratio1 µg per 1e6 cells

Details

Detection NotesFlow cytometry
Fixation DetailsRBC lysis (ammonium chloride)
Wash DescriptionPBS w/ 1% BCS

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glut4 Antibody (NBP1-49533C) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Glut4 Antibody (NBP1-49533C)

Discover related pathways, diseases and genes to Glut4 Antibody (NBP1-49533C). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Glut4.

Glucose Transporter 4 (GLUT4, SLC2A4)
GLUT4 is an insulin-sensitive glucose transporter that facilitates insulin-stimulated glucose uptake in adipose tissue, skeletal muscle, and cardiac tissues that specifically express this protein. It is a twelve transmembrane domain multi-pass protein...  Read full blog post.

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Recent Reviews

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5
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4
1
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2
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1
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Brad Poteat
04/02/2014
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol SLC2A4
Entrez