| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65805SS | |||
| Azide and BSA Free | NBP2-80660 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1642SS | |||
| Azide and BSA Free | NBP2-80639 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97728SS | |||
| Azide and BSA Free | NBP2-33253 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49045SS | |||
| Azide and BSA Free | NBP2-80658 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-89726SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0700A488-100 | |||
| Alexa Fluor 546 | DDX0700A546-100 | |||
| Alexa Fluor 647 | DDX0700A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-07035SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24588SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77668SS | |||
| Azide and BSA Free | NBP2-80903 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40686-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7276APC | |||
| Biotin | NB7276B | |||
| DyLight 350 | NB7276UV | |||
| DyLight 405 | NB7276V | |||
| DyLight 488 | NB7276G | |||
| DyLight 550 | NB7276R | |||
| DyLight 594 | NB7276DL594 | |||
| DyLight 650 | NB7276C | |||
| DyLight 680 | NB7276FR | |||
| DyLight 755 | NB7276IR | |||
| FITC | NB7278 | |||
| HRP | NB7279 | |||
| Janelia Fluor 525 | NB7276JF525 | |||
| Janelia Fluor 669 | NB7276JF669 | |||
| PE | NB7276PE | |||
| PerCP | NB7276PCP | |||
![Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36502), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Western-Blot-NB100-65805-img0015.jpg "Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36502), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CD81 Antibody (1D6) [NB100-65805] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Immunohistochemistry-Paraffin-NB100-65805-img0003.jpg)
![Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody (1D6) NB100-65805 (blue) and a matched isotype control MAB002 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-BSA-Free-Flow-Cytometry-NB100-65805-img0016.jpg)
![Western Blot: CD81 Antibody (1D6) [NB100-65805] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Western-Blot-NB100-65805-img0007.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0009.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Staining of human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0001.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0011.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0012.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0013.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0014.jpg)
![Western Blot: CD81 Antibody (1D6) - BSA Free [NB100-65805] - Characterization & internalization of M2-Exos. (A) TEM image of M2-Exos. Scale bar: 200 nm. (B) DLS measurement of M2-Exos size. (C) Western blotting assay of exosomal markers in THP-1-M2 cellular lysate & M2-Exos preparation. (D,F) Fluorescence images of 786-O & ACHN cells treated with or without PKH67-labeled M2-Exos (green). Scale bar: 50 μm. (E,G) Three-dimensional confocal reconstruction of 786-O & ACHN cells treated with PKH67-labeled M2-Exos (green). (H) Fluorescence staining analyzing the internalization of M2-Exos by 786-O cells over 12 h. Scale bar: 10 μm. CT: control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35328425), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65805_mouse-monoclonal-cd81-antibody-1d6-310202415382443.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] (NB100-1642) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0014.jpg "Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] (NB100-1642) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS-1 Cells with CD31/PECAM-1 Antibody (MEC 7.46) NB100-1642(blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Flow-Cell-Surface-NB100-1642-img0005.jpg)
![Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Immunohistochemistry for vessel staining (CD31) and beta-amyloid comparing 13- and 24-month old arcAbeta mice. Overviews (scale bars = 200 um) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnagi.2015.00241/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunohistochemistry-NB100-1642-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] conjugated to Alexa Fluor 488 (NB100-1642AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with at 5.0 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] conjugated to DyLight 550 (NB100-1642R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0016.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) and PE-conjugated F4/80 for 2 hours at the room temperature. Image using the Alexa Fluor 647 format of this antibody.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0012.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC 7.46] Antibody NB100-1642AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Flow-Cytometry-NB100-1642-img0015.jpg)
![Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Immunohistochemistry for vessel staining (CD31/PECAM-1) and beta-amyloid comparing 13- and 24-month old non-transgenic littermates. Overviews (scale bars = 200 um) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnagi.2015.00241/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunohistochemistry-NB100-1642-img0010.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM-1 Antibody (MEC 7.46) was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Flow-Cytometry-NB100-1642-img0006.jpg)
![Western Blot: TLR5 Antibody (85B152.5) [NBP1-97728] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Western-Blot-NBP1-97728-img0003.jpg "Western Blot: TLR5 Antibody (85B152.5) [NBP1-97728] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.")
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the FITC conjugate of NBP1-97728. Staining of TLR5 in 10^6 cells using 0.5 ugs of FITC-conjugated antibody: A) Intracellular flow testing of human Ramos cells and B) cell surface flow testing of mouse RAW cells. The shaded (or black) histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Flow-Cytometry-NBP1-97728-img0009.jpg)
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow analysis of TLR5 in Ramos cells using 1 ug of TLR5 antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control antibody; red represents TLR5 antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Flow-Cytometry-NBP1-97728-img0002.jpg)
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow analysis of TLR5 in 10^6 human lymphocytes using 0.5 ug of NB200-571. The shaded histogram represents cells without antibody, green represents isotype control (BD), and red represents TLR5 antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Flow-Cytometry-NBP1-97728-img0006.jpg)
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Flow-Cytometry-NBP1-97728-img0007.jpg)
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-85B152-5-Flow-Cytometry-NBP1-97728-img0008.jpg)
![Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - TLR expression in USSCs. Flow cytometry detection of TLRs in USSCs using TLR5 antibody. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20196781/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-97728_mouse-monoclonal-tlr5-antibody-85b152-5-25520231539519.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Flow-Cytometry-NBP1-49045-img0014.jpg "Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.")
![Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-Paraffin-NBP1-49045-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0006.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blood leukocytes.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Flow-Cytometry-NBP1-49045-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0011.jpg)
![Immunohistochemistry: CD8 Antibody (53-6.7) [NBP1-49045] - Histopathological analysis of spinal cord in EAE mice. Representative spinal cord sections from wild-type (WT), Asic1-/- , and Asic2-/- mice at 45 days after immunization. Sections of the spinal cord anterior horn were stained with hematoxylin and eosin (E&H) to assess inflammation and immunostained for MCHII, CD4+, CD8+ infiltrating cells. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/ejn.14302), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-NBP1-49045-img0016.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) - BSA Free [NBP1-49045] - Analysis of isolated mouse splenic CD8+ T cells using CD8 antibody (53-6.7) [Allophycocyanin] (NBP1-49045APC). Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-BSA-Free-Flow-Cytometry-NBP1-49045-img0017.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-Frozen-NBP1-49045-img0008.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-Frozen-NBP1-49045-img0015.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine splenocytes by multiple staining.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Flow-Cytometry-NBP1-49045-img0009.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in human spleen tissue using anti-CD8 alpha antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-49045_rat-monoclonal-cd8-antibody-53-6-7-immunohistochemistry-paraffin-2210202583119.jpg)
![Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Analysis using Azide/BSA FREE version of NB600-587. Membrane blocked in 1% BSA-TBS-T 30 min RT, 1:1000 dilution in 1% BSA-TBS-T 4C.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody---Azide-Free-Western-Blot-NB600-587-img0012.jpg "Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Analysis using Azide/BSA FREE version of NB600-587. Membrane blocked in 1% BSA-TBS-T 30 min RT, 1:1000 dilution in 1% BSA-TBS-T 4C.")
![Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Expression of TNF-alpha, IL-1beta and NF-kappaB in the brains of MCAO rats. Western blot confirmed that protein levels of TNF-alpha increased as early as 4 h after reperfusion, further increased at 24 h, peaked at 72 h and decreased at 7 days, respectively (*P<0.001, compared with the controls; #P<0.001 compared with the previous time point). Five rats in each group were used for western blot (n=5), sham, sham-operated control rats Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014586), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody---Azide-Free-Western-Blot-NB600-587-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody - Azide Free [NB600-587] - LMP2 knockdown by shRNA reduced the expression of TNF-alpha and IL-1beta after MCAO. Immunofluorescence labeling revealed that both TNF-alpha and IL-1beta immunoreactivity was significantly upregulated in the ipsilateral ischemic hemisphere compared with sham-operated animals. In contrast, LMP2 knockdown by shRNA significantly reduced the expression of TNF-alpha and IL-1beta. Five rats in each group were used for immunofluorescent assays (n=5). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014586), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NB600-587-img0015.jpg)
![Immunohistochemistry: TNF-alpha Antibody - Azide Free [NB600-587] - Staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody---Azide-Free-Immunohistochemistry-NB600-587-img0008.jpg)
![Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Expression of TNF-alpha, IL-1beta and NF-kappaB in the brains of MCAO rats. Western blot confirmed that protein levels of TNF-alpha increased as early as 4 h after reperfusion, further increased at 24 h, peaked at 72 h and decreased at 7 days, respectively (*P<0.001, compared with the controls; #P<0.001 compared with the previous time point). Five rats in each group were used for western blot (n=5), sham, sham-operated control rats Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014586), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody---Azide-Free-Western-Blot-NB600-587-img0013.jpg)
![Western Blot: S100A9 Antibody [NB110-89726] - Cells were transfected with the pCMV6-ENTRY S100A9 cDNA or the pCMV6-ENTRY control for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A9.](http://images.novusbio.com/fullsize/S100A9-Antibody-Western-Blot-NB110-89726-img0007.jpg "Western Blot: S100A9 Antibody [NB110-89726] - Cells were transfected with the pCMV6-ENTRY S100A9 cDNA or the pCMV6-ENTRY control for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A9.")
![Immunocytochemistry/Immunofluorescence: S100A9 Antibody [NB110-89726] - S100A9 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/S100A9-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89726-img0008.jpg)
![Immunohistochemistry-Paraffin: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200x magnification) of S100a9, in normal control, IgG Ab, and anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 um. Staining scores were determined by semi-quantitative optical analysis. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2017.01774/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S100A9-Antibody-Immunohistochemistry-Paraffin-NB110-89726-img0010.jpg)
![Western Blot: S100A9 Antibody [NB110-89726] - Analysis of S100A9 Antibody in DMSO treated HL60 whole cell lysates.](http://images.novusbio.com/fullsize/S100A9-Antibody-Western-Blot-NB110-89726-img0006.jpg)
![Simple Western: S100A9 Antibody [NB110-89726] - Simple Western lane view shows a specific band for S100A9 in 0.5 mg/ml of Human PBMC's lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/S100A9-Antibody-Simple-Western-NB110-89726-img0009.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202415291935.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202415371968.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202415395938.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-31020241539727.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, & (F) Cxcl5 in normal control, IgG Ab, & anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-31020241529199.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Effects of anti-S100a9 Ab administration on the azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colitis-associated cancer development. (A) Experimental procedure of the control group & the AOM/DSS group treated with IgG Ab or anti-S100a9 Ab. (B) DAI of the IgG Ab or anti-S100a9 Ab-treated AOM/DSS mice & normal controls. (C) General observation of the colorectums in mice at the end of the 13th & 18th week. (D) Colon length, tumor rate, & number of macroscopic neoplasms were statisticed at 13 & 18 weeks, individually. n = 5–9 per group. Results were representative of the three experiments performed. (E) Histopathological examination of colon sections was shown under the Pannoramic Viewer (H&E staining, upper panels: original magnification 10×, scale bar: 2000 µm; lower panels: original magnification 200×, scale bar: 100 µm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202416205192.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Effects of anti-S100a9 Ab on the frequency of neutrophils, macrophages, & dendritic cells (DCs) in the colon of the dextran sulfate sodium (DSS) mouse model. (A) Colon lamina propria cells were isolated from normal control & IgG Ab or anti-S100a9 Ab-treated DSS mice at day 6 post-DSS colitis induction. Frequencies of neutrophils, macrophages, & DCs in the colon were determined by flow cytometry. Cells were gated on CD45+CD3−CD4−CD11b+Ly6G+, CD45+CD3−CD4−CD11b+F4/80+, & CD45+CD3−CD4−CD11b+CD11c+ populations respectively. Representative flow cytometric figures were shown. The percentage of cells was presented as the mean ± SEM of four to six individual mice per group. *p < 0.05 in a one-way analysis of variance followed by Bonferroni correction. Data were representative of three independent experiments. (B) Immunohistochemical staining of myeloperoxidase (MPO), CD68, & CD11c proteins in the normal control & IgG Ab or anti-S100a9 Ab-treated colitis mice at day 6 (left panels: original magnification 40×, scale bar: 200 µm; right panels: original magnification 200×, scale bar: 50 µm). Staining scores were counted. One-way analysis of variance followed by Bonferroni correction. Results were representative of the three experiments performed. Error bars represent SD. (C) Expression of S100a9, Tnf alpha , Il1 beta , Il6, Il17a, Ifn gamma , Il12a, Il23a, Il4, & Il10 mRNA, as assessed by quantitative real-time PCR in normal control & IgG Ab, or anti-S100a9 Ab-treated colitis tissues. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202416205114.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Anti-S100a9 Ab ameliorates inflammatory response of dextran sulfate sodium (DSS)-induced colitis in mice. (A) 6 days after DSS treatment, representative H&E-stained colon sections were shown (upper panels: original magnification 40×, scale bar: 200 µm; lower panels: original magnification 100×, scale bar: 100 µm). (B) Colon inflammation, ulceration, & crypt damage were scored individually, & composite total score was scored. n = 5 per group. (C) Isolated lymphoid follicles (ILFs) area was measured at day 6. Representative TUNEL staining (D) & ethynyl-2′-deoxyuridine (EdU) staining (E) of normal mice & DSS-induced mice, which were treated with IgG Ab or anti-S100a9 Ab on day 6. The percent of positive cells was measured. At least six fields were counted per mouse. Scale bar, 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-31020241621842.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Host CP (S100A8/A9) is elevated in H. pylori infected stomach tissue.A) s100a8/s100a9 transcript abundance in RNA extracted from C57BL/6 mice infected with H. pylori PMSS1 or SS1 for 1, 2, or 3 months relative to uninfected animals as determined by real-time RT-PCR analysis. Points indicate mean relative units of transcript abundance +/− SEM (levels of s100a8 in PMSS1-infected mice compared to uninfected mice; 1 mo p = 0.0511; 2 mo p = 0.0432; 3 mo p = 0.0127; levels of s100a8 in SS1-infected mice compared to uninfected mice at 2 mo p = 0.0623 Student's t test). (B) Inflammation scores of H. pylori infected mice at 1, 2, & 3 months post infection. (C) s100a8/s100a9 transcript abundance in RNA extracted from gastric biopsies derived from human patients, which were either H. pylori-positive or H. pylori-negative (s100a8 p = 0.15; s100a9 *p = 0.05). Bars indicate mean relative units of transcript abundance +/− SEM. Each experimental group represents≥5 individuals (mice or human samples). D) Gastric samples derived from H. pylori PMSS1-infected WT mice or SS1-infected WT mice at 2 months post-infection were analyzed via immunohistochemistry using a polyclonal antibody to S100A9 (scale bars are 50 microns). E) Real-time RT-PCR was performed on gastric tissue to quantify s100a8 & s100a9 transcript abundance from WT (C57BL/6 mice) & IL-17RA-/- mice infected with PMSS1. Data represent relative units of transcript abundance +/− SEM in WT mice & IL-17RA-/- mice, *p = 0.0169 & p = 0.0143, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25330071), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202416205130.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Anti-S100a9 Ab ameliorates inflammatory response of dextran sulfate sodium (DSS)-induced colitis in mice. (A) 6 days after DSS treatment, representative H&E-stained colon sections were shown (upper panels: original magnification 40×, scale bar: 200 µm; lower panels: original magnification 100×, scale bar: 100 µm). (B) Colon inflammation, ulceration, & crypt damage were scored individually, & composite total score was scored. n = 5 per group. (C) Isolated lymphoid follicles (ILFs) area was measured at day 6. Representative TUNEL staining (D) & ethynyl-2′-deoxyuridine (EdU) staining (E) of normal mice & DSS-induced mice, which were treated with IgG Ab or anti-S100a9 Ab on day 6. The percent of positive cells was measured. At least six fields were counted per mouse. Scale bar, 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202416205132.jpg)
![Immunohistochemistry: S100A9 Antibody [NB110-89726] - Anti-S100a9 Ab ameliorates inflammatory response of dextran sulfate sodium (DSS)-induced colitis in mice. (A) 6 days after DSS treatment, representative H&E-stained colon sections were shown (upper panels: original magnification 40×, scale bar: 200 µm; lower panels: original magnification 100×, scale bar: 100 µm). (B) Colon inflammation, ulceration, & crypt damage were scored individually, & composite total score was scored. n = 5 per group. (C) Isolated lymphoid follicles (ILFs) area was measured at day 6. Representative TUNEL staining (D) & ethynyl-2′-deoxyuridine (EdU) staining (E) of normal mice & DSS-induced mice, which were treated with IgG Ab or anti-S100a9 Ab on day 6. The percent of positive cells was measured. At least six fields were counted per mouse. Scale bar, 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89726_rabbit-polyclonal-s100a9-antibody-310202416205144.jpg)
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0003.jpg "Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34")
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of R34-34-transfected Cos7 cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0002.jpg)
![Immunoprecipitation: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - R34-34 antigen from 125I-surface labelled Pre-Alp cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Immunoprecipitation-DDX0700P-100-img0001.jpg)
![Western Blot: CCL2/MCP1 Antibody [NBP1-07035] - Western blot for MCP-1 protein was analyzed from the ox-LDL-stimulated THP-1 cells. B-actin was used as loading control (D-I). Scale bar: 20 um. Bar graph indicates the mean +/- SD (n = 3). *P < 0.05 and **P < 0.01 vs. Cont group (0 ug/mL of ox-LDL). Image collected and cropped by Citeab from the following publication (SIRT1 inhibition promotes atherosclerosis through impaired autophagy. Oncotarget (2017)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-Western-Blot-NBP1-07035-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: CCL2/MCP1 Antibody [NBP1-07035] - Western blot for MCP-1 protein was analyzed from the ox-LDL-stimulated THP-1 cells. B-actin was used as loading control (D-I). Scale bar: 20 um. Bar graph indicates the mean +/- SD (n = 3). *P < 0.05 and **P < 0.01 vs. Cont group (0 ug/mL of ox-LDL). Image collected and cropped by Citeab from the following publication (SIRT1 inhibition promotes atherosclerosis through impaired autophagy. Oncotarget (2017)) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-07035] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:1000 dilution of CCL2/MCP1 antibody (NBP1-07035) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of CCL2 antigen in the cancer cells, stromal cells as well as the endothelial cells. The stroma itself showed a weak immunopositivity for CCL2. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-Immunohistochemistry-Paraffin-NBP1-07035-img0007.jpg)
![Western Blot: CCL2/MCP1 Antibody [NBP1-07035] - Inhibition of SIRT1 using EX-527 or SIRT1 siRNA transfection enhanced MCP-1 expression and foam cell formationHuman THP-1 macrophages were pretreated with EX-527 (2 uM, for 2 hrs) or SIRT1 siRNA (20 uM, for 24 hrs), and then exposed to 80 i1/4g/mL of ox-LDL for an additional 24 hrs. Western blot for SIRT1 and MCP-1 proteins were analyzed from the ox-LDL-stimulated THP-1 cells. I2-actin was used as loading control. Image collected and cropped by Citeab from the following publication (SIRT1 inhibition promotes atherosclerosis through impaired autophagy. Oncotarget (2017)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-Western-Blot-NBP1-07035-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody [NBP1-07035] - MCP1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-07035-img0006.jpg)
![Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-07035] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:1000 dilution of CCL2/MCP1 antibody (NBP1-07035) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of CCL2 antigen in the cancer cells, stromal cells as well as the endothelial cells. The stroma itself showed a weak immunopositivity for CCL2. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-Immunohistochemistry-Paraffin-NBP1-07035-img0008.jpg)
![Western Blot: CCL2/MCP1 Antibody [NBP1-07035] - CCL2 expression level was evaluated in control group and aLAG-3 group.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-Western-Blot-NBP1-07035-img0009.jpg)
![Western Blot: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - Ox-LDL induced macrophage foam cell formation, SIRT1 inhibition, autophagy impairment, & MCP-1 production in THP-1 cellsHuman THP-1 macrophages were exposed to 0, 20, 40, 60, & 80 μg/mL of ox-LDL for 24 hrs. Treated cells were photographed using light microscopy (A). The THP-1 macrophage-derived foam cell formation was determined using ORO staining method (B) & (C). Western blot for SIRT1, LC3, Beclin1, p62, & MCP-1 proteins were analyzed from the ox-LDL-stimulated THP-1 cells. beta -actin was used as loading control (D-I). Scale bar: 20 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 & **P < 0.01 vs. Cont group (0 μg/mL of ox-LDL). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07035_rabbit-polyclonal-ccl2-mcp1-antibody-310202415304238.jpg)
![Western Blot: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - Inhibition of autophagy with 3-MA led to increased foam cell formation & MCP-1 productionHuman THP-1 macrophages were pretreated with 3-MA (5 μM) for 2 hrs, & then exposed to 80 μg/mL of ox-LDL for an additional 24 hrs. Western blot for LC3, Beclin1, p62, & MCP-1 proteins were analyzed from the ox-LDL-stimulated THP-1 cells. beta -actin was used as loading control (A-E). THP-1 macrophage-derived foam cell formation was determined using ORO staining method (F-G). Scale bar: 40 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 & **P < 0.01 vs. Cont group; #P < 0.05 & ##P < 0.01 vs. 3-MA group; &P < 0.05 & &&P < 0.01 represent significant differences between ox-LDL group & 3-MA+ox-LDL group. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07035_rabbit-polyclonal-ccl2-mcp1-antibody-310202415384154.jpg)
![Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - CCL2-ir cellular distribution in ependymal layers attached to the CC. A, B CCL2-ir (CCL2 panels, arrows) & GFAP (GFAP panels, arrowheads) double immunostained structures (Merged panels, arrow-arrowheads) are seen inlaid in the ependymal layers attached to the CC in both saline & LPS injected mice. C A CCL2-ir & Iba-1 double labeled soma (Merged, arrow-arrowhead) is seen at a corner of lateral ventricle, which seems be just next to an invaginated choroid plexus between the CC & basal ganglion (framed areas & insets). D, E Hyper-ramified & amoeba like Iba-1 labeled cells (Iba-1 panel, opened arrowheads) are observed in the CC of LPS injected mice, indicating their activated states. The CCL2-ir & Iba-1 double labeled structures are also regarded in the ependymal layers (Merged, arrow-arrowheads). Scare bar = 100 µm in all A– E Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35354428), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07035_rabbit-polyclonal-ccl2-mcp1-antibody-310202416161849.jpg)
![Western Blot: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - Inhibition of autophagy using Atg5 siRNA aggravated foam cell formation & MCP-1 expressionHuman THP-1 macrophages were pretreated with Atg5 siRNA (20 μM) for 24 hrs, & then exposed to 80 μg/mL of ox-LDL for an additional 24 hrs. Western blot forAtg5, LC3, Beclin1, p62, & MCP-1 proteins were analyzed from the ox-LDL-stimulated THP-1 cells. beta -actin was used as loading control (A-F) & (I). THP-1 macrophage-derived foam cell formation was determined using ORO staining method (G) & (H). Scale bar: 40 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 & **P < 0.01 vs. NC siRNA group; #P < 0.05 & ##P < 0.01 vs. Atg5 siRNA group; &P < 0.05 & &&P < 0.01 represent significant differences between NC siRNA+ox-LDL group & Atg5 siRNA+ox-LDL group. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07035_rabbit-polyclonal-ccl2-mcp1-antibody-31020241618266.jpg)
![Western Blot: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - Inhibition of SIRT1 using EX-527 or SIRT1 siRNA transfection enhanced MCP-1 expression & foam cell formationHuman THP-1 macrophages were pretreated with EX-527 (2 μM, for 2 hrs) or SIRT1 siRNA (20 μM, for 24 hrs), & then exposed to 80 μg/mL of ox-LDL for an additional 24 hrs. Western blot for SIRT1 & MCP-1 proteins were analyzed from the ox-LDL-stimulated THP-1 cells. beta -actin was used as loading control (A-C) & (F-H). THP-1 macrophage-derived foam cell formation was determined using ORO staining method (D-E) & (I-J). Scale bar: 40 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 & **P < 0.01 vs. Cont group (NC siRNA group); #P < 0.05 & ##P < 0.01 vs. EX-527 group (SIRT1 siRNA group); &P < 0.05 & &&P < 0.01 represent significant differences between ox-LDL group (NC siRNA+ox-LDL group) & EX-527+ox-LDL group (SIRT1 siRNA+ox-LDL group). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07035_rabbit-polyclonal-ccl2-mcp1-antibody-310202416171476.jpg)
![Western Blot: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - Overexpression of SIRT1 using adenoviral transfection reversed ox-LDL-induced macrophage foam cell formation & autophagy impairment in THP-1 cellsHuman THP-1 macrophages were transfected by SIRT1 over-expressing adenovirus (HBAD-SIRT1) or NC adenovirus (HBAD-GFP) for 24 hrs & then exposed to 80 μg/mL of ox-LDL for an additional 24 hrs. The transfected THP-1 cells were observed using an inverted fluorescence microscope (A) & then were harvested for transfection efficiency analysis by Western blot method (B). THP-1 macrophage-derived foam cell formation was determined using ORO staining method (C) & (D). Western blot for LC3, Beclin1, p62, & Atg5 proteins & immunoprecipitation for acetyl-Lys Atg5 were analyzed from the ox-LDL-stimulated THP-1 cells. beta -actin was used as loading control (E-L). Scale bar: 40 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 & **P < 0.01 vs. HBAD-GFP group; #P < 0.05 & ##P < 0.01 vs. HBAD-SIRT1 group; &P < 0.05 & &&P < 0.01 represent significant differences between HBAD-GFP+ox-LDL group & HBAD-SIRT1+ox-LDL group. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07035_rabbit-polyclonal-ccl2-mcp1-antibody-310202416163742.jpg)
![Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 Antibody [NBP2-24588]. Human pancreas probed with RIPK3/RIP3 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunohistochemistry-Paraffin-NBP2-24588-img0004.jpg "Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 Antibody [NBP2-24588]. Human pancreas probed with RIPK3/RIP3 antibody at 10 ug/ml.")
![<b>Biological Strategies Validation. </b>Western Blot: RIPK3/RIP3 Antibody [NBP2-24588] - Representative western blot analysis of RIP1, RIPK3/RIP3, and beta-actin in WI-38 NCs and IR-SCs 24 h after incubation with Veh or 10 uM PL. A cell lysate of etoposide-treated Jurkat cells was used as a positive control. Image collected and cropped by CiteAb from the following publication (//www.aging-us.com/article/101100/text) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP2-24588-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RIPK3/RIP3 Antibody [NBP2-24588] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with RIPK3/RIP3 Antibody [NBP2-24588] at a 1:200 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used at a 1:1000 dilution as a co-stain and detected with DyLight 550 (Red) at a 1:500 dilution. Nuclei were detected with DAPI (Blue) at 2.0ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24588-img0003.jpg)
![Western Blot: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 antibody [NBP2-24588]. Human tissue blot probed with 5 ug/ml of RIP3 antibody: 1) brain, 2) heart, 3) small intestine, 4) kidney, 5) liver, 6) lung, 7) skeletal muscle, 8) stomach, 9) spleen, 10) ovary, 11) testis, 12) placenta probed at 5 ug/ml, 13) Ramos cell lysate probed at 7 ug/ml. Theoretical molecular weight 56.7 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP2-24588-img0005.jpg)
![Western Blot: RIPK3/RIP3 Antibody - BSA Free [NBP2-24588] - HOIP limits the activity of TRAIL‐induced apoptosis‐ & necroptosis‐mediating signalling complexesControl & HOIP KO HeLa cells were treated with FLAG‐lz‐TRAIL (500 ng/ml) for the indicated times. The TRAIL complex I was immunoprecipitated via anti‐FLAG beads. Complex II was isolated by immunoprecipitating caspase‐8 from complex I‐depleted lysates. Western blot was performed using the indicated antibodies. * indicates unspecific bands.WT & HOIP‐deficient TNF KO MEFs, pre‐treated for 1 h with zVAD, were stimulated with iz‐TRAIL (1 μg/ml) for the indicated times. FADD‐containing complexes were immunoprecipitated & analysed by Western blot.Data information: See also Fig EV2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28258062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24588_rabbit-polyclonal-ripk3-rip3-antibody-31020241534394.jpg)
![Western Blot: RIPK3/RIP3 Antibody - BSA Free [NBP2-24588] - The catalytic activity of HOIP contributes to preventing TRAIL‐induced apoptosis but is dispensable for preventing necroptosisLysates from control or HOIP KO K562 cells reconstituted w/ empty vector, HOIP WT or HOIP C885S analysed by WB.HOIP KO K562 cells reconstituted w/ empty vector, HOIP WT or HOIP C885S, pre‐treated w/ zVAD and/or Nec‐1s for 1 h as indicated, treated w/ iz‐TRAIL (1 μg/ml) for 24 h. Percentage of cell death determined by flow cytometry after PI labelling (n = 5; mean ± SEM). *P < 0.05, **P < 0.01; statistics performed using ANOVA.Control & HOIP KO K562 cells reconstituted w/ empty vector, HOIP WT or HOIP C885S treated w/ iz‐TRAIL (100 ng/ml) for the indicated times. Lysates analysed w/ the indicated antibodies.Lysates from TNF KO HOIP KO MEFs reconstituted w/ empty vector, HOIP WT or HOIP C885S analysed by WB.TNF KO HOIP KO MEFs reconstituted w/ empty vector, HOIP WT or HOIP C885S, pre‐treated w/ zVAD & Nec‐1s for 1 h as indicated, treated w/ iz‐TRAIL (1 μg/ml) for 24 h. Percentage of cell death determined by flow cytometry after PI labelling (n = 4; mean ± SEM). *P < 0.05, ***P < 0.001; statistics performed using ANOVA.TNF KO HOIP KO MEFs reconstituted w/ empty vector, pre‐treated w/ zVAD and/or the RIPK3 inhibitor GSK'872 for 1 h as indicated, treated w/ iz‐TRAIL (1 μg/ml) for 24 h. Percentage of cell death determined by flow cytometry after PI labelling (n = 3; mean ± SEM). *P < 0.05; statistics performed using t‐test.TNF KO HOIP KO MEFs reconstituted w/ empty vector, HOIP WT or HOIP C885S, pre‐treated w/ zVAD for 1 h, treated w/ iz‐TRAIL (1 μg/ml) for the indicated times. FADD‐containing complexes immunoprecipitated & analysed by WB. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28258062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24588_rabbit-polyclonal-ripk3-rip3-antibody-310202416114733.jpg)
![Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Total protein from human Colon, Thyroid and Stomach was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Panendothelial Cell Antigen (NB100-77668) in blocking buffer and detected with an anti-rat HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Panendothelial-Cell-Antigen-Antibody-MECA-32-Western-Blot-NB100-77668-img0009.jpg "Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Total protein from human Colon, Thyroid and Stomach was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Panendothelial Cell Antigen (NB100-77668) in blocking buffer and detected with an anti-rat HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunohistochemistry-Paraffin: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Panendothelial Cell Antigen (MECA-32) was detected in immersion fixed paraffin-embedded sections of human spleen. Antibody at 1:500 for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the endothelial cells.](http://images.novusbio.com/fullsize/Panendothelial-Cell-Antigen-Antibody-MECA-32-Immunohistochemistry-Paraffin-NB100-77668-img0008.jpg)
![Flow Cytometry: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Analysis using the PerCP conjugate of NB100-77668. Staining of bEND.3 cells with MECA-32 AF488.](http://images.novusbio.com/fullsize/Panendothelial-Cell-Antigen-Antibody-MECA-32-Flow-Cytometry-NB100-77668-img0007.jpg)
![Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Mouse embryonic cell lysate (A). Antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/Panendothelial-Cell-Antigen-Antibody-MECA-32-Western-Blot-NB100-77668-img0006.jpg)
![Immunohistochemistry-Frozen: CD163 Antibody (EDHu-1) [NB110-40686] - Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.](http://images.novusbio.com/fullsize/CD163-Antibody-EDHu-1-Immunohistochemistry-Frozen-NB110-40686-img0007.jpg)
![Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in a formalin fixed paraffin embedded section of canine lymph node. NB110-40686 was diluted to 0.25ug per mL and was left on the tissue section for 30m at room temperature. Section underwent 20m of heat induced epitope retrieval in a vegetable steamer using a citrate buffer. No signal was detected in sections that did not undergo HIER. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb110-40686_mouse-monoclonal-cd163-antibody-edhu-1-immunohistochemistry-paraffin-722024125639..png)
![Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of human tonsil. NB110-40686 was diluted to 250 ng/mL and left on tissue sections for 30min at room temperature. Secondary was horse anti mouse HRP polymer. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb110-40686_mouse-monoclonal-cd163-antibody-edhu-1-immunohistochemistry-paraffin-294202415725..png)
![Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of equine spleen. NB110-40686 was diluted to 250ng per mL and left on tissue sections for 30min at room temperature. Secondary was horse anti mouse HRP polymer. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb110-40686_mouse-monoclonal-cd163-antibody-edhu-1-immunohistochemistry-paraffin-1052024154747..jpg)
![Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of feline lymph node. NB110-40686 was diluted to 250ng per mL and left on tissue sections for 30min at room temperature. Secondary was horse anti mouse HRP polymer. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb110-40686_mouse-monoclonal-cd163-antibody-edhu-1-immunohistochemistry-paraffin-105202415547..png)
![Immunocytochemistry/ Immunofluorescence: CD163 Antibody (EDHu-1) [NB110-40686] - Non-repaired & repaired VML injured muscles present sustained putative macrophage infiltration. Representative histologic images labeled with CD163 (pan macrophage marker) & wheat germ agglutinin (WGA) & DAPI (nuclei). (a) For each of the sham muscle sections individual & merged images are presented. (b) Examples for each group, specifically the non-, SIS-, UMB-, & Hya-reparied are shown. Scale bar is 150 µm; all images are at the same magnification & follow the same location pattern displayed specifically in Fig. 4e. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030619), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40686_mouse-monoclonal-cd163-antibody-edhu-1-31020241538410.jpg)