LAP (TGF-beta 1) Antibody (7F6)

Images

 
Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)
Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lymphoid tissue tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.
Flow Cytometry: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Flow cytometric analysis of A549 cells using LAP (TGF-beta 1) mouse mAb (green) and negative control (red).
Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis of human stomach tissue (A) and human small intestine tissue (B) using TGF beta 1 antibody (NBP2-22114) at 2 ug/ml.
Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Human tonsil. Image from verified customer review.
Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.
ELISA: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clone
7F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

LAP (TGF-beta 1) Antibody (7F6) Summary

Immunogen
Purified recombinant fragment of human TGF beta 1 (amino acids 62-197) expressed in E. coli. [UniProt# P01137]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TGFB1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
IHC-P reported in a verified customer review. Use in Immunohistochemistry reported in scientific literature (PMID: 30147399).
Theoretical MW
44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 3.7
using
NBP2-22114 in the following applications:

Publications
Read Publications using
NBP2-22114 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26851347). Rat reactivity reported in scientific literature (PMID: 30147399).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for LAP (TGF-beta 1) Antibody (7F6)

  • CED
  • DPD1
  • LAP (TGFbeta 1)
  • LAP (TGF-beta 1)
  • LAP
  • TGFB
  • TGFB1
  • TGFbeta
  • transforming growth factor beta 1

Background

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3797
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
NB100-56479
Species: Hu, Mu, Rt, Po, Bv, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-82847
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24951
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
6507-IL/CF
Species: Hu
MAB2029
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NBP2-22114
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, IHC, IHC-P

Publications for LAP (TGF-beta 1) Antibody (NBP2-22114)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-Fr
(1)
IHC-P
(2)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP2-22114 Applications Species
Wang J, Lee CJ, Deci MB et al. MiR-101a loaded extracellular nanovesicles as bioactive carriers for cardiac repair Nanomedicine Apr 8 2020 [PMID: 32278100] (IHC, IHC-Fr, Mouse) IHC, IHC-Fr Mouse
Wang X, Jian X, Dou J et al. Decreasing Microtubule Actin Cross-Linking Factor 1 Inhibits Melanoma Metastasis by Decreasing Epithelial to Mesenchymal Transition Cancer Manag Res Jan 29 2020 [PMID: 32099463] (WB, Mouse) WB Mouse
Reed M, Luissint AC, Azcutia V et al. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo Nat Commun Nov 1 2019 [PMID: 31676794] (WB, Mouse) WB Mouse
Hendrijantini N, Kusumaningsih T, Rostiny R et al. A potential therapy of human umbilical cord mesenchymal stem cells for bone regeneration on osteoporotic mandibular bone Eur J Dent Aug 28 2018 [PMID: 30147399] (IHC, Rat) IHC Rat
Awad SM, Ismail SA, Sayed DS et al. Suppression of transforming growth factor-beta1 expression in keloids after cryosurgery. Cryobiology. Mar 10 2017 [PMID: 28288795] (IHC-P, Human) IHC-P Human
Hosseini H, Li Y, Kanellakis P et al. TollLike Receptor (TLR) 4 and MyD88 are Essential for Atheroprotection by Peritoneal B1a B Cells. Journal of the American Heart Association. Nov 14 2016 [PMID: 27930350] (Mouse) Mouse
Derricott H, Jones RL, Greenwood SL et al. Characterizing Villitis of Unknown Etiology and Inflammation in Stillbirth. The American Journal of Pathology. 2016 Apr 18 [PMID: 26851347] (IHC-P, Mouse) IHC-P Mouse
Makboul M, Makboul R, Abdelhafez AH et al. Evaluation of the effect of fractional CO2 laser on histopathological picture and TGF-b1 expression in hypertrophic scar. J Cosmet Dermatol. 2014 Sep 01 [PMID: 25196683]

Reviews for LAP (TGF-beta 1) Antibody (NBP2-22114) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-22114:
Filter by Applications
WB
(1)
IHC-P
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot LAP (TGF-beta 1) NBP2-22114
Enlarge
2
reviewed by:
Shatovisha Dey
WB Mouse 10/02/2020
View

Summary

ApplicationWestern Blot
Sample TestedMouse Pancreas
SpeciesMouse
Lot384830
  4
reviewed by:
Anonymous
IHC-P Human 10/28/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman kidney
SpeciesHuman
Lot130520
Immunohistochemistry-Paraffin LAP (TGF-beta 1) NBP2-22114
Enlarge
5
reviewed by:
Hayley Derricott
IHC-P Human 04/04/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
Lot130102

Blocking

Blocking DetailsNon-immune block (swine serum, human serum, TBS-T 0.1%), 30min at RT

Primary Anitbody

Dilution Ratio10µg/mL (1:100) in non-immune block incubated at 4 degrees overnight

Secondary Antibody

Secondary DescriptionBiotinylated goat anti-mouse IgG

Details

Detection NotesDAB, 9 minutes. Negative control mouse IgG matched concentration to primary.
Fixation DetailsFormalin-fixed, wax-embedded, 10min heat-mediated citrate buffer Ag retrieval (20min cool), 10min peroxidase block
Wash Description1 TBS wash 5min, 2 TBS-T 0.6% washes 5min, 1 TBS wash 5min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LAP (TGF-beta 1) Antibody (NBP2-22114). (Showing 1 - 1 of 1 FAQs).

  1. Info regarding this product NBP2-22114SS, does this product cross reacts with Latent TGF-beta 1 also?
    • Based on the immunogen sequence, we predict it would cross react.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22114AF350
Alexa Fluor 405 NBP2-22114AF405
Alexa Fluor 488 NBP2-22114AF488
Alexa Fluor 532 NBP2-22114AF532
Alexa Fluor 594 NBP2-22114AF594
Alexa Fluor 647 NBP2-22114AF647
Alexa Fluor 700 NBP2-22114AF700
Alexa Fluor 750 NBP2-22114AF750
Biotin NBP2-22114B
DyLight 350 NBP2-22114UV
DyLight 405 NBP2-22114V
DyLight 488 NBP2-22114G
DyLight 550 NBP2-22114R
DyLight 594 NBP2-22114DL594
DyLight 650 NBP2-22114C
DyLight 680 NBP2-22114FR
DyLight 755 NBP2-22114IR
FITC NBP2-22114F
HRP NBP2-22114H
Janelia Fluor 549 NBP2-22114JF549
Janelia Fluor 646 NBP2-22114JF646

Additional LAP (TGF-beta 1) Products

Bioinformatics Tool for LAP (TGF-beta 1) Antibody (NBP2-22114)

Discover related pathways, diseases and genes to LAP (TGF-beta 1) Antibody (NBP2-22114). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LAP (TGF-beta 1) Antibody (NBP2-22114)

Discover more about diseases related to LAP (TGF-beta 1) Antibody (NBP2-22114).
 

Pathways for LAP (TGF-beta 1) Antibody (NBP2-22114)

View related products by pathway.

PTMs for LAP (TGF-beta 1) Antibody (NBP2-22114)

Learn more about PTMs related to LAP (TGF-beta 1) Antibody (NBP2-22114).

Blogs on LAP (TGF-beta 1)

There are no specific blogs for LAP (TGF-beta 1), but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3.7
5
1
4
1
3
0
2
1
1
0

Shatovisha Dey
10/02/2020
Application: WB
Species: Mouse

 
Anonymous
10/28/2015
Application: IHC-P
Species: Human

Hayley Derricott
04/04/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol TGFB1
Entrez
Uniprot