S100A9 Antibody

Images

 
Western Blot: S100A9 Antibody [NB110-89726] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY S100A9 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by ...read more
Immunocytochemistry/ Immunofluorescence: S100A9 Antibody [NB110-89726] - S100A9 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200x magnification) of ...read more
Western Blot: S100A9 Antibody [NB110-89726] - Analysis of S100A9 Antibody in DMSO treated HL60 whole cell lysates.
Simple Western: S100A9 Antibody [NB110-89726] - Simple Western lane view shows a specific band for S100A9 in 0.5 mg/ml of Human PBMC's lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Host CP (S100A8/A9) is elevated in H. pylori infected stomach tissue. D) Gastric samples derived from H. pylori PMSS1-infected WT mice or SS1-infected WT mice at 2 months post-infection were analyzed via ...read more
Cell cycle block under PHD3 depletion is accompanied by p27 induction. a PHD3 depletion induces a cell cycle block in G0/G1. HeLa and renal cell adenocarcinoma cells (786-O) were transfected with control (siScr) or PHD3 ...read more
Anti-S100a9 Ab ameliorates inflammatory response of dextran sulfate sodium (DSS)-induced colitis in mice. (A) 6 days after DSS treatment, representative H&E-stained colon sections were shown (upper panels: original ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

S100A9 Antibody Summary

Immunogen
Full length human S100A9 protein [Swiss-Prot# P06702] expressed in E. coli.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S100A9
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry reported in scientific literature (PMID 25792748)
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 21653680)
  • Simple Western 1:100
  • Western Blot 1:1000
Application Notes
In Western blot, a band is seen at approx. 16 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
S100A9 Overexpression Lysate
Publications
Read Publications using
NB110-89726 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for S100A9 Antibody

  • CAGBMigration inhibitory factor-related protein 14
  • Calgranulin B
  • calgranulin-B
  • Calprotectin L1H subunit
  • CFAGMRP-14,60B8AG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP-14
  • MRP14Leukocyte L1 complex heavy chain
  • NIF
  • P14
  • protein S100-A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100 calcium binding protein A9
  • S100 calcium-binding protein A9 (calgranulin B)
  • S100 calcium-binding protein A9
  • S100A9

Background

S100A9 (S100 calcium-binding protein A9) is a member of S-100 family (family named so as its members are 100% solubile in ammonium sulfate at normal pH) and it exist as a homodimer or as heterodimer/heterotetramer with S100A8. S100A9 is a calcium binding protein with several post-translation modifications as well as multiple ligands, and when bound to calcium, it binds to arachidonic acid, cytoskeletal elements (e.g. keratin intermediate filaments), RAGE (receptor for advanced glycation endproducts), TLR4, CD36, MMPs, fibronectin, and heparin sulfate glycosaminoglycans for serving diverse biological functions. Localization of S100A9 varies according to cell type/disease state, however, it predominantly localize to cytoplasm, from where it translocates to cytoskeleton/cell membrane upon elevation of intracellular calcium levels. During neutrophil activation or endothelial adhesion of monocytes, it gets secreted via a microtubule-mediated alternative pathway. S100A9 is expressed in normal cell types such as myeloid cells, but its expression levels are increased in conditions of inflammatory diseases (RA, SLE, cystic fibrosis, inflammatory bowel disease, Crohn's disease, Sjogren's syndrome, and progressive systemic sclerosis) as well as in several types of cancers including gastric, esophageal, colon, pancreatic, bladder, ovarian, thyroid, breast and skin cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A9 Antibody (NB110-89726)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: IF/IHC, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IF/IHC
(4)
IHC
(2)
IHC-Fr
(1)
IHC-P
(5)
WB
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(11)
Rat
(2)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB110-89726 Applications Species
Yoshikawa T, Takeichi T, Hirabayashi T et al. IL-17 axis is a significant driver of skin inflammation in Card14 mutant pityriasis rubra pilaris model mice Research Square 2023-02-02 (IHC, Mouse) IHC Mouse
Fong LY, Huebner K, Jing R et al. Zinc treatment reverses and anti-Zn-regulated miRs suppress esophageal carcinomas in vivo Proceedings of the National Academy of Sciences of the United States of America 2023-05-16 [PMID: 37155893]
Manzanares LD, David J, Ren X et al. Atovaquone attenuates experimental colitis by reducing neutrophil infiltration of colonic mucosa Frontiers in Pharmacology 2022-10-14 [PMID: 36313299] (IHC) IHC
Li Z, Zhang X, Liu C Et al. Macrophage-Biomimetic Nanoparticles Ameliorate Ulcerative Colitis through Reducing Inflammatory Factors Expression Journal of innate immunity 2021-11-01 [PMID: 34724662] (IHC-P, Mouse) IHC-P Mouse
Ancey PB, Contat C, Boivin G et al. GLUT1 Expression in Tumor-Associated Neutrophils Promotes Lung Cancer Growth and Resistance to Radiotherapy Cancer Research 2021-05-01 [PMID: 33753374]
Bui TM, Butin-Israeli V, Wiesolek HL et al. Neutrophils Alter DNA Repair Landscape to Impact Survival and Shape Distinct Therapeutic Phenotypes of Colorectal Cancer Gastroenterology 2021-03-19 [PMID: 33753103] (Mouse) Mouse
Kwak T, Wang F, Deng H et al. Distinct Populations of Immune-Suppressive Macrophages Differentiate from Monocytic Myeloid-Derived Suppressor Cells in Cancer Cell reports 2020-12-29 [PMID: 33378668]
Mabuchi S, Komura N, Sasano T et Al. Pretreatment tumor-related leukocytosis misleads positron emission tomography-computed tomography during lymph node staging in gynecological malignancies Nat Commun 2020-03-13 [PMID: 32170086] (IF/IHC, Human) IF/IHC Human
Kingsley MK, Bhat BV, Badhe BA et al. Narciclasine improves outcome in sepsis among neonatal rats via inhibition of calprotectin and alleviating inflammatory responses Sci Rep 2020-02-19 [PMID: 32076015] (IHC-P, IF/IHC, Rat) IHC-P, IF/IHC Rat
Duan X, Liu X, Liu N et al. Inhibition of keratinocyte necroptosis mediated by RIPK1/RIPK3/MLKL provides a protective effect against psoriatic inflammation Cell Death Dis 2020-02-19 [PMID: 32075957] (WB, Human, Mouse) WB Human, Mouse
Show All 22 Publications.

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Product General Protocols

View specific protocols for S100A9 Antibody (NB110-89726): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S100A9 Antibody (NB110-89726). (Showing 1 - 3 of 3 FAQs).

  1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
  3. We would like to know detail information about immunocytochemistry and immunofluorescence. Could you provide protocol and other image for our reference?
    • For our ICC testing of this product, our lab tested in A431 cells. This image is visible on our datasheet here: Datasheet. The lab followed the following protocol: Protocol, and used the antibody at a 1:100 dilution.
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Bioinformatics

Gene Symbol S100A9
Entrez
Uniprot