Western Blot: S100A9 Antibody [NB110-89726] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY S100A9 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by ...read more
Immunocytochemistry/ Immunofluorescence: S100A9 Antibody [NB110-89726] - S100A9 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Western Blot: S100A9 Antibody [NB110-89726] - Analysis of S100A9 Antibody in DMSO treated HL60 whole cell lysates.
Simple Western: S100A9 Antibody [NB110-89726] - Simple Western lane view shows a specific band for S100A9 in 0.5 mg/ml of Human PBMC's lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Hu, Mu, RtSpecies Glossary
WB, Simple Western, ICC/IF, IHC, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Western Blot 1:1000
Simple Western 1:100
This S100A9 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~16 kDa..Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 21653680)In Simple Western only 10-15 uL of the recommended dilution is used per data point. Use in Immunohistochemistry reported in scientific literature (PMID 25792748) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
16 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB110-89726 in the following applications:
CAGBMigration inhibitory factor-related protein 14
Calprotectin L1H subunit
MRP14Leukocyte L1 complex heavy chain
S100 calcium binding protein A9 (calgranulin B)
S100 calcium binding protein A9
S100 calcium-binding protein A9 (calgranulin B)
S100 calcium-binding protein A9
S100A9 (S100 calcium-binding protein A9) is a member of S-100 family (family named so as its members are 100% solubile in ammonium sulfate at normal pH) and it exist as a homodimer or as heterodimer/heterotetramer with S100A8. S100A9 is a calcium binding protein with several post-translation modifications as well as multiple ligands, and when bound to calcium, it binds to arachidonic acid, cytoskeletal elements (e.g. keratin intermediate filaments), RAGE (receptor for advanced glycation endproducts), TLR4, CD36, MMPs, fibronectin, and heparin sulfate glycosaminoglycans for serving diverse biological functions. Localization of S100A9 varies according to cell type/disease state, however, it predominantly localize to cytoplasm, from where it translocates to cytoskeleton/cell membrane upon elevation of intracellular calcium levels. During neutrophil activation or endothelial adhesion of monocytes, it gets secreted via a microtubule-mediated alternative pathway. S100A9 is expressed in normal cell types such as myeloid cells, but its expression levels are increased in conditions of inflammatory diseases (RA, SLE, cystic fibrosis, inflammatory bowel disease, Crohn's disease, Sjogren's syndrome, and progressive systemic sclerosis) as well as in several types of cancers including gastric, esophageal, colon, pancreatic, bladder, ovarian, thyroid, breast and skin cancers.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Langle Y, Sandes E, Belgorosky D. La expresion de S100A9, vinculada con el oxidontrico, es un marcador de mal pronostico enpacientes con cancer de vejiga, siendo su inhibicionun posible blanco terapeutico. Rev. Arg. de Urol. 2014 Mar 01
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Product General Protocols
View specific protocols for S100A9 Antibody (NB110-89726):
FAQs for S100A9 Antibody (NB110-89726). (Showing 1 - 3 of 3 FAQs).
Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
We would like to know detail information about immunocytochemistry and immunofluorescence. Could you provide protocol and other image for our reference?
For our ICC testing of this product, our lab tested in A431 cells. This image is visible on our datasheet here: Datasheet. The lab followed the following protocol: Protocol, and used the antibody at a 1:100 dilution.