S100A9 Antibody

Images

 
Western Blot: S100A9 Antibody [NB110-89726] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY S100A9 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by ...read more
Immunocytochemistry/ Immunofluorescence: S100A9 Antibody [NB110-89726] - S100A9 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Western Blot: S100A9 Antibody [NB110-89726] - Analysis of S100A9 Antibody in DMSO treated HL60 whole cell lysates.
Simple Western: S100A9 Antibody [NB110-89726] - Simple Western lane view shows a specific band for S100A9 in 0.5 mg/ml of Human PBMC's lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

S100A9 Antibody Summary

Immunogen
Full length human S100A9 protein [Swiss-Prot# P06702] expressed in E. coli.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S100A9
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This S100A9 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at approx. 16 kDa.Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 21653680) Use in Immunohistochemistry reported in scientific literature (PMID 25792748)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
S100A9 Lysate (NBL1-15660)
Publications
Read Publications using
NB110-89726 in the following applications:

  • IHC
    1 publication
  • 3 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for S100A9 Antibody

  • CAGBMigration inhibitory factor-related protein 14
  • Calgranulin B
  • calgranulin-B
  • Calprotectin L1H subunit
  • CFAGMRP-14,60B8AG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP-14
  • MRP14Leukocyte L1 complex heavy chain
  • NIF
  • P14
  • protein S100-A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100 calcium binding protein A9
  • S100 calcium-binding protein A9 (calgranulin B)
  • S100 calcium-binding protein A9
  • S100A9

Background

S100A9 (S100 calcium-binding protein A9) is a member of S-100 family (family named so as its members are 100% solubile in ammonium sulfate at normal pH) and it exist as a homodimer or as heterodimer/heterotetramer with S100A8. S100A9 is a calcium binding protein with several post-translation modifications as well as multiple ligands, and when bound to calcium, it binds to arachidonic acid, cytoskeletal elements (e.g. keratin intermediate filaments), RAGE (receptor for advanced glycation endproducts), TLR4, CD36, MMPs, fibronectin, and heparin sulfate glycosaminoglycans for serving diverse biological functions. Localization of S100A9 varies according to cell type/disease state, however, it predominantly localize to cytoplasm, from where it translocates to cytoskeleton/cell membrane upon elevation of intracellular calcium levels. During neutrophil activation or endothelial adhesion of monocytes, it gets secreted via a microtubule-mediated alternative pathway. S100A9 is expressed in normal cell types such as myeloid cells, but its expression levels are increased in conditions of inflammatory diseases (RA, SLE, cystic fibrosis, inflammatory bowel disease, Crohn's disease, Sjogren's syndrome, and progressive systemic sclerosis) as well as in several types of cancers including gastric, esophageal, colon, pancreatic, bladder, ovarian, thyroid, breast and skin cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100A9 Antibody (NB110-89726)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(3)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB110-89726 Applications Species
Ikubo K, Yamanishi K, Doe N et al. Molecular analysis of the mouse brain exposed to chronic mild stress: The influence of hepatocyte nuclear factor 4a on physiological homeostasis. Mol Med Rep May 11 2017 [PMID: 28498421]
Zhang X, Ai F, Li X et al. Inflammation-induced S100A8 activates Id3 and promotes colorectal tumorigenesis Int. J. Cancer 2015 Jul 01 [PMID: 26135667] (IHC-P, Mouse, Human)

Details:
S100A9 antibody was used for IHC-P on tissue specimens from human colorectal cancer patients and AOM/DSS-induced colitis-associated cancers/CAC mouse model (Figure 1).
IHC-P Mouse, Human
Chernov AV, Dolkas J, Hoang K et al. Calcium-binding proteins S100A8 and S100A9 initiate the early inflammatory program in injured peripheral nerve. J. Biol. Chem. 2015 Mar 19 [PMID: 25792748] (WB, IHC, Rat) WB, IHC Rat
Gaddy Ja, Radin Jn, Loh Jt et al. The Host Protein Calprotectin Modulates the Helicobacter pylori cag Type IV Secretion System via Zinc Sequestration. PLoS Pathog. 2014 Oct 01 [PMID: 25330071] (IHC-P, Mouse) IHC-P Mouse
Sumi D, Shimizu Y, Himeno S. Involvement of Nrf2 activation in the upregulation of S100A9 by exposure to inorganic arsenite. Int J Mol Med. 2013 Jan [PMID: 23165982]
Pitteri SJ, Kelly-Spratt KS, Gurley KE et al. Tumor microenvironment-derived proteins dominate the plasma proteome response during breast cancer induction and progression. Cancer Res 2011 Aug 1 [PMID: 21653680] (IHC-P, Mouse) IHC-P Mouse
Langle Y, Sandes E, Belgorosky D. La expresion de S100A9, vinculada con el oxidontrico, es un marcador de mal pronostico enpacientes con cancer de vejiga, siendo su inhibicionun posible blanco terapeutico. Rev. Arg. de Urol. 2014 Mar 01

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Product General Protocols

View specific protocols for S100A9 Antibody (NB110-89726): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S100A9 Antibody (NB110-89726). (Showing 1 - 3 of 3 FAQs).

  1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
  3. We would like to know detail information about immunocytochemistry and immunofluorescence. Could you provide protocol and other image for our reference?
    • For our ICC testing of this product, our lab tested in A431 cells. This image is visible on our datasheet here: Datasheet. The lab followed the following protocol: Protocol, and used the antibody at a 1:100 dilution.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol S100A9
Entrez
Uniprot