CCL2/MCP1 Antibody

Images

 
Western Blot: CCL2/MCP1 Antibody [NBP1-07035] - CCL2 expression level was evaluated in control group and aLAG-3 group.
Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody [NBP1-07035] - MCP1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-07035] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:1000 dilution of CCL2/MCP1 antibody (NBP1-07035) ...read more
Western Blot: CCL2/MCP1 Antibody [NBP1-07035] - TNF alpha treated PC12 lysates.
Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-07035] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:1000 dilution of CCL2/MCP1 antibody (NBP1-07035) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

CCL2/MCP1 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of rat MCP1 (within residues 15-40). [Swiss-Prot# P14844]
Localization
Secreted, secretory vesicles
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Block/Neutralize
Application Notes
This MCP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~16 kDa. In ICC/IP secretory vesicles staining was observed in HeLa cells. WB and B/N applications are reported in research publications with PMIDs 22402584 and 22778093 respectively.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CCL2/MCP1 Overexpression Lysate
CCL2/MCP1 Knockout HeLa Cell Lysate
Control Peptide
CCL2/MCP1 Peptide (NBP1-07035PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-07035 in the following applications:

Publications
Read Publications using
NBP1-07035 in the following applications:

  • B/N
    1 publication
  • 2 publications
  • IHC
    1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CCL2/MCP1 Antibody

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

MCP-1 (monocyte chemoattracctant protein-1, also called C-C motif chemokine 2 or CCL2) is a secreted protein belonging to the intercrine beta (chemokine CC) family and specifically acts as a chemotactic factor to attract monocytes, CD4+ T cells, NK cells, and CCR2 expressing cells, but not neutrophils. Both hematopoietic and non-hematopoietic cells that include monocytes/macrophages, dendritic cells, astrocytes, endothelial cells and fibroblasts express MCP-1, and monocytes/macrophages are the major source of MCP-1 in peripheral blood. In addition to expression at acute inflammation sites, MCP-1 is upregulated in chronic inflammation, including autoimmune disease, atherosclerosis, cancer, and chronic infection. MCP-1 has been implicated in both pro-inflammatory and anti-inflammatory responses, and is a potential intervention point for the treatment of various inflammatory/autoimmune diseases (multiple sclerosis, atherosclerosis, insulin-resistant diabetes, rheumatoid arthritis and lupus nephritis), cancers (breast and prostate cancer) and HIV-1/AIDS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL2/MCP1 Antibody (NBP1-07035)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: B/N, ICC/IF, IHC, WB.


Filter By Application
B/N
(1)
ICC/IF
(2)
IHC
(1)
WB
(5)
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Filter By Species
Human
(3)
Mouse
(4)
Rat
(2)
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Showing Publications 1 - 9 of 9.
Publications using NBP1-07035 Applications Species
Yang X, Wei J, He Y et al. SIRT1 inhibition promotes atherosclerosis through impaired autophagy Oncotarget 2017 Aug 01 [PMID: 28881659] (ICC/IF, Human) ICC/IF Human
Deng W, Mao L, Yu G et al. LAG-3 confers poor prognosis and its blockade reshapes antitumor response in head and neck squamous cell carcinoma. Oncoimmunology 2016 Oct 07 [PMID: 27999760] (WB, Mouse) WB Mouse
Choi JA, Chung YR, Byun HR et al. The anti-ALS drug riluzole attenuates pericyte loss in the diabetic retinopathy of streptozotocin-treated mice. Toxicol Appl Pharmacol 2017 Jan 15 [PMID: 27939241] (Mouse) Mouse
Lamont EA, O'Grady SM, Davis WC et al. Infection with Mycobacterium avium subsp. paratuberculosis Results in Rapid Interleukin-1 beta Release and Macrophage Transepithelial Migration Infect Immun 2012 Sep [PMID: 22778093] (B/N, Human) B/N Human
Fet NG, Fiebeler A, Klinge U et al. Reduction of activated macrophages after ischaemia-reperfusion injury diminishes oxidative stress and ameliorates renal damage Nephrol Dial Transplant 2012 Mar 8 [PMID: 22402584] (IHC, Rat) IHC Rat
Wu J, Dou Y, Ladiges W Adverse neurological effects of short-term sleep deprivation in aging mice are prevented by SS31 peptide bioRxiv Jun 6 2020 (WB, Mouse) WB Mouse
Chen I-Chen, Lin Yu-Tsai, Huang Jhy-Shrian et al. Decreased Ambient Oxygen Tension Alters the Expression of Endothelin-1, iNOS and cGMP in Rat Alveolar Macrophages. International Journal of Medical Sciences 2019 Feb 28 [PMID: 30911278] (WB, Rat) WB Rat
Alghamdi TA, Batchu SN, Hadden MJ, et al. Histone H3 Serine 10 Phosphorylation Facilitates Endothelial Activation in Diabetic Kidney Disease Diabetes 2018 Dec 01 [PMID: 30213824] (WB, Mouse) WB Mouse
Verzola D, Milanesi S, Bertolotto M et al. Myostatin mediates abdominal aortic atherosclerosis progression by inducing vascular smooth muscle cell dysfunction and monocyte recruitment. Scientific Reports 2017 [PMID: 28406165] (WB, ICC/IF, Human) WB, ICC/IF Human

Review for CCL2/MCP1 Antibody (NBP1-07035) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-07035:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CCL2/MCP1 NBP1-07035
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reviewed by:
Anonymous
WB Mouse 10/23/2017
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Summary

ApplicationWestern Blot
Sample Tested Mouse Head and neck squamous cell carcinoma (HNSCC)
SpeciesMouse
LotNBP1-07035

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CCL2/MCP1 Antibody (NBP1-07035) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-07035AF350
Alexa Fluor 405 NBP1-07035AF405
Alexa Fluor 488 NBP1-07035AF488
Alexa Fluor 532 NBP1-07035AF532
Alexa Fluor 594 NBP1-07035AF594
Alexa Fluor 647 NBP1-07035AF647
Alexa Fluor 700 NBP1-07035AF700
Alexa Fluor 750 NBP1-07035AF750
Biotin NBP1-07035B
DyLight 350 NBP1-07035UV
DyLight 405 NBP1-07035V
DyLight 488 NBP1-07035G
DyLight 550 NBP1-07035R
DyLight 594 NBP1-07035DL594
DyLight 650 NBP1-07035C
DyLight 680 NBP1-07035FR
DyLight 755 NBP1-07035IR
FITC NBP1-07035F
HRP NBP1-07035H
Janelia Fluor 549 NBP1-07035JF549
Janelia Fluor 646 NBP1-07035JF646

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Bioinformatics Tool for CCL2/MCP1 Antibody (NBP1-07035)

Discover related pathways, diseases and genes to CCL2/MCP1 Antibody (NBP1-07035). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CCL2/MCP1 Antibody (NBP1-07035)

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Pathways for CCL2/MCP1 Antibody (NBP1-07035)

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PTMs for CCL2/MCP1 Antibody (NBP1-07035)

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Research Areas for CCL2/MCP1 Antibody (NBP1-07035)

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Blogs on CCL2/MCP1.

MCP-1 - chemoattractant protein involed in monocyte migration and infiltration
Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/23/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CCL2