CCL2/MCP1 Antibody

Images

 
Western Blot: CCL2/MCP1 Antibody [NBP1-07035] - TNF alpha treated PC12 lysates.
Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody [NBP1-07035] - MCP1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-07035] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:1000 dilution of CCL2/MCP1 antibody (NBP1-07035) ...read more
Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-07035] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:1000 dilution of CCL2/MCP1 antibody (NBP1-07035) ...read more

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, B/N, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

CCL2/MCP1 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of rat MCP1 (within residues 15-40). [Swiss-Prot# P14844]
Localization
Secreted, secretory vesicles
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Blocking/Neutralizing
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes
This MCP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~16 kDa. In ICC/IP secretory vesicles staining was observed in HeLa cells. IHC-Fr and B/N applications are reported in research publications with PMIDs 22402584 and 22778093 respectively. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
CCL2/MCP1 Lysate (NBL1-08849)
Control Peptide
CCL2/MCP1 Peptide (NBP1-07035PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-07035 in the following applications:

Publications
Read Publications using
NBP1-07035 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CCL2/MCP1 Antibody

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

MCP-1 (monocyte chemoattracctant protein-1, also called C-C motif chemokine 2 or CCL2) is a secreted protein belonging to intercrine beta (chemokine CC) family and it specifically act as a chemotactic factor to attracts monocytes, CD4+ T cells, NK cells as well as for CCR2 expressing cells, but not neutrophils. Both hematopoietic and non-hematopoietic cells that include monocytes/macrophages, dendritic cells, astrocytes, endothelial cells and fibroblasts express MCP-1, and monocytes/macrophages are the major source of MCP-1 in peripheral blood. Besides, expression at acute inflammation sites, MCP-1 is upregulated in chronic inflammation, including autoimmune disease, atherosclerosis, cancer, and chronic infection. MCP-1 has been implicated in both pro-inflammatory and anti-inflammatory responses, and is a potential intervention point for the treatment of various inflammatory/autoimmune diseases (multiple sclerosis, atherosclerosis, insulin-resistant diabetes, rheumatoid arthritis and lupus nephritis), cancers (breast and prostate cancer) and HIV-1/AIDS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL2/MCP1 Antibody (NBP1-07035)(4)

Review for CCL2/MCP1 Antibody (NBP1-07035) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-07035:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot CCL2/MCP1 NBP1-07035
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reviewed by:
Anonymous
WB Mouse 10/23/2017
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Summary

ApplicationWestern Blot
Sample Tested Mouse Head and neck squamous cell carcinoma (HNSCC)
SpeciesMouse
LotNBP1-07035

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CCL2/MCP1 Antibody (NBP1-07035) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-07035AF405
Alexa Fluor 488 Labeled NBP1-07035AF488
Alexa Fluor 647 Labeled NBP1-07035AF647
Alexa Fluor 700 Labeled NBP1-07035AF700
Biotin Labeled NBP1-07035B
DyLight 405 Labeled NBP1-07035V
DyLight 488 Labeled NBP1-07035G
DyLight 550 Labeled NBP1-07035R
DyLight 650 Labeled NBP1-07035C
FITC Labeled NBP1-07035F
HRP Labeled NBP1-07035H

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Bioinformatics Tool for CCL2/MCP1 Antibody (NBP1-07035)

Discover related pathways, diseases and genes to CCL2/MCP1 Antibody (NBP1-07035). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CCL2/MCP1 Antibody (NBP1-07035)

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PTMs for CCL2/MCP1 Antibody (NBP1-07035)

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Blogs on CCL2/MCP1.

MCP-1 - chemoattractant protein involed in monocyte migration and infiltration
Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C...  Read full blog post.

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Recent Reviews

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5
1
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1
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Anonymous
10/23/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CCL2