| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47386SS | |||
| Azide and BSA Free | NBP2-80650 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-534SS | |||
| Azide and BSA Free | NBP2-80947 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56580SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47518SS | |||
| Azide and BSA Free | NBP2-80610 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56698SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-654SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25274 | |||
| BSA Free | NBP2-27332SS | |||
| Azide and BSA Free | NBP2-25274SS | |||
| Azide and BSA Free | NBP2-80982 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1327SS | |||
| Azide and BSA Free | NBP2-80626 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56571SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22114SS | |||
| Azide and BSA Free | NBP2-80827 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-39474SS | |||
| Azide and BSA Free | NBP2-80562 | |||
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0011.jpg "Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).")
![Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunohistochemistry-Paraffin-NBP1-47386-img0014.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Flow-Cytometry-NBP1-47386-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD44 Antibody [8E2F3] conjugated to DyLight 550 (NBP1-47386R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0018.jpg)
![Western Blot: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - Generation and characterization of immortalized primary keratinocytes (IPKs) from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. Western blot analysis of pSTAT3, STAT3, and CD44 (NBP1-47386) in primary keratinocytes and IPKs from both genotypes. The A431 cells were used as positive controls. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29955047/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-BSA-Free-Western-Blot-NBP1-47386-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD44 Antibody [8E2F3] conjugated to Alexa Fluor 647 (NBP1-47386AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0019.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Flow-Cytometry-NBP1-47386-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0010.jpg)
![Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Flow-Intracellular-NBP1-47386-img0016.jpg)
![Immunoprecipitation: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - HAP1 lysates were prepared and immunoprecipitation was performed using 1.0 ug of the CD44 Antibody (NBP1-47386) pre-coupled to either protein G or protein A Sepharose beads. Ability of the antibodies to capture CD44 antigen was first assessed by comparing the level of CD44 antigen from the starting material (SM) to its level remaining in the unbound fractions (UB). Anti-CD44 antigen at 1/2000 was used for each immunoblot. Immunoprecipitate for CD44 Antibody (NBP1-47386) that showed depleted CD44 antigen in the UB can be seen. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-BSA-Free-Immunoprecipitation-NBP1-47386-img0020.jpg)
![Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-HSn-7D2-Immunohistochemistry-Frozen-NB600-534-img0003.jpg "Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.")
![Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-HSn-7D2-Flow-Cytometry-NB600-534-img0002.jpg)
![Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Sialoadhesin-Antibody-HSn-7D2-Immunohistochemistry-Paraffin-NB600-534-img0001.jpg)
![Western Blot: TLR4 Antibody [NB100-56580] - Immunoblot data examining response of primary macrophages from WT and miR-223 knockout mice (KO) to LPS and combined IFNG/LPS; 50ug of whole cell lysate was loaded per lane and run on a denaturing SDS PAGE gel. Densitometry and statistics are presented in (D). All values are expressed as +/- SEM; * indicates p<0.05 via student t-test. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0165962) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NB100-56580-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: TLR4 Antibody [NB100-56580] - Immunoblot data examining response of primary macrophages from WT and miR-223 knockout mice (KO) to LPS and combined IFNG/LPS; 50ug of whole cell lysate was loaded per lane and run on a denaturing SDS PAGE gel. Densitometry and statistics are presented in (D). All values are expressed as +/- SEM; * indicates p<0.05 via student t-test. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0165962) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TLR4 Antibody [NB100-56580] - Analysis of TLR4 in mouse kidney tissue using TLR4 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-Paraffin-NB100-56580-img0004.jpg)
![Western Blot: TLR4 Antibody [NB100-56580] - Analysis of TLR4. (A) 1 ug/lane partial recombinant mouse TLR4 protein (antibody at 2 ug/mL) and (B) RAW cell lysate and (C) Daudi cell lysate (antibody at 5 ug/mL on tumor cell lysate).](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NB100-56580-img0005.jpg)
![Western Blot: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of whole cell lysates from HeLa, A549, NTERA2 and MCF-7 using Calreticulin antibody clone 1G6A7. The antibody generated a specific band of Calreticulin protein at ~48 kDa molecular weight position.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Western-Blot-NBP1-47518-img0017.jpg "Western Blot: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of whole cell lysates from HeLa, A549, NTERA2 and MCF-7 using Calreticulin antibody clone 1G6A7. The antibody generated a specific band of Calreticulin protein at ~48 kDa molecular weight position.")
![Flow (Intracellular): Calreticulin Antibody (1G6A7) [NBP1-47518] - Intracellular staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Calreticulin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - Antibody was tested in HeLa cells at a 1:250 against DyLight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Immunocytochemistry-Immunofluorescence-NBP1-47518-img0008.jpg)
![Western Blot: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis using Calreticulin mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Calreticulin cDNA (2).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Western-Blot-NBP1-47518-img0005.jpg)
![Immunohistochemistry-Paraffin: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of paraffin-embedded human placenta tissues using anti-Calreticulin mAb.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Immunohistochemistry-Paraffin-NBP1-47518-img0002.jpg)
![Flow Cytometry: Calreticulin Antibody (1G6A7) [NBP1-47518] - An intracellular stain was performed on A431 cells with Calreticulin (1G6A7) Antibody NBP1-47518 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of 3T3-L1 cells using anti-Calreticulin mAb (green). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Immunocytochemistry-Immunofluorescence-NBP1-47518-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of SKBR-3 (left) and A549 (right) cells using anti-Calreticulin mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Immunocytochemistry-Immunofluorescence-NBP1-47518-img0007.jpg)
![Flow (Intracellular): Calreticulin Antibody (1G6A7) [NBP1-47518] - A549 cells stained with NBP1-47518PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0026.jpg)
![Flow (Intracellular): Calreticulin Antibody (1G6A7) [NBP1-47518] - U-937 cells stained with NBP1-47518F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0027.jpg)
![Flow (Intracellular): Calreticulin Antibody (1G6A7) [NBP1-47518] - Jurkat cells stained with NBP1-47518APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0028.jpg)
![Flow Cytometry: Calreticulin Antibody (1G6A7) [NBP1-47518] - An intracellular stain was performed on HeLa cells with Calreticulin Antibody [1G6A7] NBP1-47518AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0034.jpg)
![Western Blot: MyD88 Antibody [NB100-56698] - The ability of PTX to induce the IL-1 Beta IL-6 cascade depends on the integrity of its multimeric structure and enzymatic activity.Western blots revealing the presence of mature IL-1 Beta, cleaved Casp1, TLR4 and Myd88 in peritoneal fluid or peritoneal cell lysates from mice killed 6 h after injection of PBS, wild-type PTX or PTXmut. Arrowheads indicate the cleaved forms. Beta-actin was used as loading control. Sample size: 8 per group. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004150) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NB100-56698-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: MyD88 Antibody [NB100-56698] - The ability of PTX to induce the IL-1 Beta IL-6 cascade depends on the integrity of its multimeric structure and enzymatic activity.Western blots revealing the presence of mature IL-1 Beta, cleaved Casp1, TLR4 and Myd88 in peritoneal fluid or peritoneal cell lysates from mice killed 6 h after injection of PBS, wild-type PTX or PTXmut. Arrowheads indicate the cleaved forms. Beta-actin was used as loading control. Sample size: 8 per group. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004150) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MyD88 Antibody [NB100-56698] - Raw264.7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MyD88 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MyD88-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56698-img0008.jpg)
![Immunohistochemistry-Paraffin: MyD88 Antibody [NB100-56698] - Tissue section of human liver using at 1:100 dilution. This antibody generated a very specific cytoplasmic staining in the hepatocytes as well as the Kupffer cells (hepatic macrophages), and the latter showed the most intense staining among all other cell types in the section.](http://images.novusbio.com/fullsize/MyD88-Antibody-Immunohistochemistry-Paraffin-NB100-56698-img0005.jpg)
![Western Blot: MyD88 Antibody [NB100-56698] - Analysis of MyD88 in human spleen cell lysate using 0.5 ug/ml of NB100-56698.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NB100-56698-img0003.jpg)
![Immunohistochemistry-Paraffin: MyD88 Antibody [NB100-56698] - Tissue section of human liver using at 1:100 dilution. This antibody generated a very specific cytoplasmic staining in the hepatocytes as well as the Kupffer cells (hepatic macrophages), and the latter showed the most intense staining among all other cell types in the section.](http://images.novusbio.com/fullsize/MyD88-Antibody-Immunohistochemistry-Paraffin-NB100-56698-img0004.jpg)
![Western Blot: MyD88 Antibody - BSA Free [NB100-56698] - TLR & other LPS-response elements in LPS-stimulated HSCs.(A) Microarray data show time-dependent changes in the indicated transcripts. For clarity control gene expression at 1 & 24h is offset to 0.5h & 24.5h respectively. (B) A representative Western blot (left pane) & densitometric analysis (right panel) of the indicated molecules at 24h following stimulation with 10 ng/ml LPS. (C) qPCR analysis of the indicated molecules with p values showing statistical differences. The values (B,C) shown are form 3 separate determinations from different batches of HSCs. Statistical significance was derived from student’s t-test using Microsoft-excel program. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0082159), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56698_rabbit-polyclonal-myd88-antibody-310202415371913.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-654] - Effects of in vitro hypoxia on the expression of mouse HIF-1 alpha in HIF-1 alpha+/- and HIF-1 alpha+/+ astrocytes. Confluent astrocyte monolayers of both cell types were exposed to a 6 h in vitro hypoxia. HIF-1 alpha mRNA expression was determined by RT-PCR as described in Materials and Methods. Each bar represents the mean +/- SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisks and number sign indicate significant difference (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Western-Blot-NB100-654-img0009.jpg "<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody [NB100-654] - Effects of in vitro hypoxia on the expression of mouse HIF-1 alpha in HIF-1 alpha+/- and HIF-1 alpha+/+ astrocytes. Confluent astrocyte monolayers of both cell types were exposed to a 6 h in vitro hypoxia. HIF-1 alpha mRNA expression was determined by RT-PCR as described in Materials and Methods. Each bar represents the mean +/- SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisks and number sign indicate significant difference (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license.")
![<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody [NB100-654] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. Western blots using nuclear proteins show that both hypoxia (H) and CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha+/+ and HIF-1 alpha+/- cells. There was no HIF-1 alpha protein detected in control cells. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Western-Blot-NB100-654-img0008.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-654] - IHC analysis of a formalin-fixed paraffin-embedded tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF-1 alpha antibody NB100-654 at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the section was further counterstained using hematoxylin. The tested section depicted mainly a diffused cytoplasmic staining but there were some cells which showed nuclear signal also (representing hypoxic cells).](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-654-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-654] - HIF1-alpha analysis using NB100-654. Image submitted from verified customer review.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Western-Blot-NB100-654-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-654] - Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using NB100-654.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Western-Blot-NB100-654-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody [NB100-654] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Simple-Western-NB100-654-img0005.jpg)
![Gel Super Shift Assays: HIF-1 alpha Antibody [NB100-654] - Mouse HIF-1 alpha+/+ and HIF-1 alpha+/- cells were exposed to hypoxia or 125 uM CoCl2 for 6 h, nuclear extracts were then prepared and EMSA carried out. HIF-1 isolated from hypoxia or CoCl2-treated cells bound to the wild-type probe (lanes #1-6) but not to the mutant probe (lanes #7-12). HIF-1/DNA complex was detected in hypoxia (H)-or CoCl2 (Co)-treated cells (lanes #2, 3, 5, 6) but not in control (C) cells (lanes #1, 4). More complex (darker band) was seen in hypoxia-or CoCl2-treated HIF-1 alpha+/+ cells (lanes #2, 3) than that in hypoxia-or CoCl2-treated HIF-1 alpha+/- cells (lanes #5, 6). Supershift assay showed that the HIF-1/DNA complex was shifted up in the presence of wild-type (wt) oligo probe and 4 ug HIF-1 alpha antiboby (lanes #13 & 14). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Gel-Super-Shift-Assays-NB100-654-img0011.jpg)
![Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. (A) The cells were incubated in the presence or absence of 125 μM CoCl2 for 6 hr. HIF-1 alpha mRNA expression was determined by RT-PCR. Each bar represents the mean ± SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisk & number sign indicate significant difference compared to relevant controls (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). (B) Western blots using nuclear proteins show that both hypoxia (H) & CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha +/+ & HIF-1 alpha +/- cells. There was no HIF-1 alpha protein detected in control cells (C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/17474992), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-654_rabbit-polyclonal-hif-1-alpha-antibody-31020241529191.jpg)
![Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Aberrant HIF1 alpha expression in PDAC cancer associated fibroblastsA. The level of MCT4 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. B. The level of MCT1 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. C. HIF alpha & MCT4 levels were determined by immunoblotting following the knockdown of HIF1 alpha with RNAi or the induction of hypoxia through the use of CoCl2. D. The stability of HIF1 alpha was evaluated in CAFs following treatment with cycloheximide. E. HIF1 alpha was detected by immunoblotting in the presence of cycloheximide in the absence or presence of the proteasome inhibitor MG132. F. HIF1 alpha protein levels were determined in the absence & presence of DMOG. G. RNA levels of the indicated genes as determined by microarray analyses (**p < 0.01 for tumor cell line vs. CAF). H. MG132 was used to interrogate the synthetic rate of HIF1 alpha protein in the indicated cell lines. I. The expression of HIF1 alpha target genes was evaluated in the indicated cell lines by microarray analysis (**p < 0.01 for tumor cell vs. CAF). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623078), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-654_rabbit-polyclonal-hif-1-alpha-antibody-31020241553714.jpg)
![Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Aberrant HIF1 alpha expression in PDAC cancer associated fibroblastsA. The level of MCT4 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. B. The level of MCT1 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. C. HIF alpha & MCT4 levels were determined by immunoblotting following the knockdown of HIF1 alpha with RNAi or the induction of hypoxia through the use of CoCl2. D. The stability of HIF1 alpha was evaluated in CAFs following treatment with cycloheximide. E. HIF1 alpha was detected by immunoblotting in the presence of cycloheximide in the absence or presence of the proteasome inhibitor MG132. F. HIF1 alpha protein levels were determined in the absence & presence of DMOG. G. RNA levels of the indicated genes as determined by microarray analyses (**p < 0.01 for tumor cell line vs. CAF). H. MG132 was used to interrogate the synthetic rate of HIF1 alpha protein in the indicated cell lines. I. The expression of HIF1 alpha target genes was evaluated in the indicated cell lines by microarray analysis (**p < 0.01 for tumor cell vs. CAF). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623078), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-654_rabbit-polyclonal-hif-1-alpha-antibody-31020241682154.jpg)
![Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Aberrant HIF1 alpha expression in PDAC cancer associated fibroblastsA. The level of MCT4 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. B. The level of MCT1 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. C. HIF alpha & MCT4 levels were determined by immunoblotting following the knockdown of HIF1 alpha with RNAi or the induction of hypoxia through the use of CoCl2. D. The stability of HIF1 alpha was evaluated in CAFs following treatment with cycloheximide. E. HIF1 alpha was detected by immunoblotting in the presence of cycloheximide in the absence or presence of the proteasome inhibitor MG132. F. HIF1 alpha protein levels were determined in the absence & presence of DMOG. G. RNA levels of the indicated genes as determined by microarray analyses (**p < 0.01 for tumor cell line vs. CAF). H. MG132 was used to interrogate the synthetic rate of HIF1 alpha protein in the indicated cell lines. I. The expression of HIF1 alpha target genes was evaluated in the indicated cell lines by microarray analysis (**p < 0.01 for tumor cell vs. CAF). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623078), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-654_rabbit-polyclonal-hif-1-alpha-antibody-310202415523946.jpg)
![Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-654] - Aberrant HIF1 alpha expression in PDAC cancer associated fibroblastsA. The level of MCT4 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. B. The level of MCT1 was determined in the indicated established pancreatic cancer cell lines & CAF cultures by immunoblotting. C. HIF alpha & MCT4 levels were determined by immunoblotting following the knockdown of HIF1 alpha with RNAi or the induction of hypoxia through the use of CoCl2. D. The stability of HIF1 alpha was evaluated in CAFs following treatment with cycloheximide. E. HIF1 alpha was detected by immunoblotting in the presence of cycloheximide in the absence or presence of the proteasome inhibitor MG132. F. HIF1 alpha protein levels were determined in the absence & presence of DMOG. G. RNA levels of the indicated genes as determined by microarray analyses (**p < 0.01 for tumor cell line vs. CAF). H. MG132 was used to interrogate the synthetic rate of HIF1 alpha protein in the indicated cell lines. I. The expression of HIF1 alpha target genes was evaluated in the indicated cell lines by microarray analysis (**p < 0.01 for tumor cell vs. CAF). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623078), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-654_rabbit-polyclonal-hif-1-alpha-antibody-31020241553515.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - THP-1 cells were stained with TLR7 (4G) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0008.jpg "Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - THP-1 cells were stained with TLR7 (4G) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.")
![Flow (Intracellular): TLR7 Antibody (4G6) [NBP2-27332] - Analysis using the PE conjugate of NBP2-27332. Staining of TLR7 in 10^6 human BDCM cells (red) and 0.5 ug of mouse IgG1 isotype control (green). this antibody was used for this test.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Intracellular-NBP2-27332-img0009.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis using Azide Free version of NBP2-27332. THP-1 cells were stained with TLR7 (4G) NBP2-25274 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0012.jpg)
![Western Blot: TLR7 Antibody (4G6) - BSA Free [NBP2-27332] - The protein of TLR7 were pull down by miR-138-biotin / miR-mut-138-biotin with Streptavidin agarose beads in BV2 cells. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC7710131/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-BSA-Free-Western-Blot-NBP2-27332-img0017.jpg)
![Immunohistochemistry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of TLR7 [4G6] antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Immunohistochemistry-NBP2-27332-img0016.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Ramos cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0015.jpg)
![Western Blot: TLR7 Antibody (4G6) [NBP2-27332] - TLR7 antibodies tested at 2 ug/ml on recombinant partial hTLR7 protein (amino acids 562-839). NB100-56682 and NBP2-24767 immunogen sequences are not present in recombinant protein; *NB100-56588 is not recommended for use in western blot.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Western-Blot-NBP2-27332-img0005.jpg)
![Western Blot: TLR7 Antibody (4G6) [NBP2-27332] - Analysis using Azide Free version of NBP2-27332. Human TLR7 antibody in Ramos and transfected 293T lysate using TLR7 monoclonal antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Western-Blot-NBP2-27332-img0011.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis of TLR7 in human monocytes using 2 ug of TLR7 monoclonal antibody (red) and 2 ug of mouse IgG1 isotype control antibody (green). TLR intracellular flow kit was used for this test, and an anti-mouse IgG PE conjugated secondary antibody .](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0002.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Daudi cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0013.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Raji cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0014.jpg)
![Western Blot: TLR7 Antibody (4G6) - BSA Free [NBP2-27332] - ADEV miR‐138 interacts with murine TLR7 in the endosomes. (A) Representative fluorescence images of mouse primary microglial cells incubated with Exo‐Fect+ADEVs+Cy5‐miR138 or Exo‐Fect+Cy5‐miR138 (without ADEVs) or Exo‐Fect+ADEVs+miR138 (unstained) for 30 min followed by immunostaining of (A) an early endosome marker (EEA1, Green) & (B) TLR7 (Green). Exo‐fect+ADEVs+Cy5‐miR138, Cy5‐miR138 were loaded into ADEVs using by Exo‐Fect transfection kit; Exo‐fect+Cy5‐miR138, Cy5‐miR138 were loaded using by Exo‐Fect transfection kit (without ADEVs); Exo‐fect+ADEVs+miR138, unstained miR138 were loaded into ADEVs using Exo‐Fect transfection kit; Bars, 50 μm (n = 3). (C) TLR7 was immunoprecipitated from BV2 cells by IgG / TLR7 / LAMP1 antibody, followed by assessment of miR138 / U6 expression by real‐time PCR. One‐way ANOVA followed by Bonferroni's post hoc test was used to determine the statistical significance among multiple groups (n = 3). (D) TLR7 was immunoprecipitated from HEK‐Null / HEK‐TLR7 cells by IgG/TLR7 antibody, followed by assessment of miR138 expression by real‐time PCR. One‐way ANOVA followed by Bonferroni's post hoc test was used to determine the statistical significance among multiple groups (n = 3). (E) The protein of TLR7 were pull down by miR‐138‐biotin / miR‐mut‐138‐biotin with Streptavidin agarose beads in BV2 cells. All data are presented as mean ± SD or SEM of three individual experiments. *,P < 0.05; **,P < 0.01; ***,P < 0.001 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33304479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27332_mouse-monoclonal-tlr7-antibody-4g6-310202416114724.jpg)
![Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunohistochemistry-NB600-1327-img0012.jpg "Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody.")
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Characterization of microglia cultures by multiple staining with CD11b Antibody (M1/70.15) [NB600-1327] (panels a. & c.) and anti GFAP (panels b & d). Nuclei appear blue due to 4',6-diamidino-2-phenylindole (DAPI) counter staining.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0013.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - A surface stain was performed on Raw264.7 cells with CD11b (M1/70.15) Antibody [NB600-1327R] (blue) and a matched isotype control (orange), both conjugated to DyLight 550. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] (NB600-1327) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1327-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] conjugated to Alexa Fluor 488 (NB600-1327AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1327-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemistry/Immunofluorescence (ICC/IF) analysis of RAW264.7 cells using CD11b antibody (M1/70.15) [NB600-1327] with Dylight 488 conjugated secondary (green). Nuclei and tubulin were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent staining in untreated and treated mouse microglia cells using CD11b Antibody (M1/70.15) [NB600-1327]. Image from a verified customer review.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0015.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent analysis in mouse primary microglia. Cells were treated with lysophosphatidic acid (LPA) or LPA and an LPAR5 inhibitor in order to investigate the expression of iNOS after 24 hours. CD11b Antibody (M1/70.15) [NB600-1327] was used in a dilution 1:100 in order to stain primary murine microglia. Cy2 (green) and Cy3 (red) were used as secondary antibodies.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] conjugated to Alexa Fluor 647 (NB600-1327AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1327-img0019.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327].](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0004.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]: RPE - Alexa Fluor 750](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0007.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages using the Alexa Fluor (R) 488 conjugate of CD11b Antibody (M1/70.15) [NB600-1327AF488].](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0014.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) and (B) show mRNA and protein levels of TLR3 in sham and MI hearts. n = 4 mice/group. Data are means +/- S.D. AP < 0.01 versus sham. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR3-Antibody-Western-Blot-NB100-56571-img0009.jpg "<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) and (B) show mRNA and protein levels of TLR3 in sham and MI hearts. n = 4 mice/group. Data are means +/- S.D. AP < 0.01 versus sham. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Frozen: TLR3 Antibody [NB100-56571] - Frozen mouse eye tissue sections stained with TLR3, a retinal pigment epithelium (RPE) marker and DAPI. Top panel: TLR3 (red) colocalized with the RPE (green) marker. Bottom panel: Tissue stained with isotype control (no TLR3 Ab), RPE and DAPI. Data courtesy of Kleinman et al.](http://images.novusbio.com/fullsize/TLR3-Antibody-Immunohistochemistry-Frozen-NB100-56571-img0002.jpg)
![Flow Cytometry: TLR3 Antibody [NB100-56571] - Analysis using the FITC conjugate of NB100-56571. Staining of TLR3 in Balb/c mouse splenocytes using this antibody at 1 ug/10^6 cells. Green represents rabbit IgG isotype control this antibody; red represents anti-TLR3 antibody.](http://images.novusbio.com/fullsize/TLR3-Antibody-Flow-Cytometry-NB100-56571-img0008.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Analysis of TLR3 in mouse spleen tissue lysate using NB100-56571 at 2 ug/ml.](http://images.novusbio.com/fullsize/TLR3-Antibody-Western-Blot-NB100-56571-img0005.jpg)
![Immunohistochemistry: TLR3 Antibody [NB100-56571] - Pig lung poisoned through aspiration with gastric juice and organophosphate (dimethoate EC40) after 48 hours of IC ventilation. Image from verified customer review.](http://images.novusbio.com/fullsize/TLR3-Antibody-Immunohistochemistry-NB100-56571-img0006.jpg)
![Flow Cytometry: TLR3 Antibody [NB100-56571] - Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes and mouse RAW cells using TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-Flow-Cytometry-NB100-56571-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TLR3 Antibody [NB100-56571] - siRNA was transfected at 50 nM for 48 hrs, and poly(I:C) was added at 100 ug/ml for 4 hrs before cell harvest. Negative control (NC) siRNA served as control. RNAiMAX transfection reagent was used in all the siRNA experiments. Panel shows the knockdown effects of siRNAs. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR3-Antibody-Knockdown-Validated-NB100-56571-img0010.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415523938.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-3102024165731.jpg)
![Immunohistochemistry: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415535158.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - TLR3‐knockout (TLR3‐KO) attenuated cardiac autophagy induced by MI. The infarct & remote tissues were separately sampled from the left ventricle after 4 weeks of MI. Accordingly, anterior & posterior tissues of the left ventricle were sampled from sham hearts as controls. (A) Representative Western blot images & quantitative analyses of autophagy markers. n = 4–8/group. Quantitative data are fold changes of WT‐sham. aP < 0.05, AP < 0.01 versus respective WT‐sham; bP < 0.05, BP < 0.01 versus respective WT‐MI. (B) Representative electron microphotographs of ultrathin sections of resin‐embedded heart biopsies. Arrows indicate autophagic vacuoles. (C) Lysates of infarct tissue were immunoprecipitated (IP), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG served as a control. Green arrows indicate non‐specific bands. Representative images were taken from four independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415525214.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - A TLR3 agonist polyinosinic‐polycytidylic acid (poly(I:C)) induced autophagy in cultured cardiomyocytes through a TRIF‐dependent pathway. (A) Poly(I:C) increased autophagy markers in cultured H9c2 rat ventricular cells. (B) Poly(I:C) stimulated autophagosome formation but did not affect autophagic flux. Primary cultured neonatal rat ventricular myocytes (NRVMs) were transfected with a tandem mRFP‐GFP‐LC3 adenovirus for 24 hrs, followed by treatment with poly(I:C) (100 μg/ml, 4 hrs). Autophagosomes & autolysosomes were, respectively, visualized as yellow‐ & red‐only punctas under a confocal microscope. (C) An autophagic flux inhibitor chloroquine (CQ) induced accumulations of LC3‐II & p62/SQSTM1 proteins in H9c2 myocytes receiving poly(I:C) (100 μg/ml, 4 hrs). CQ was applied at 10 μM, immediately prior to poly(I:C). (D) Effects of indicated siRNA on poly(I:C)‐induced changes in autophagy markers in NRVMs. All the siRNAs were transfected at 50 nM for 48 hrs, & poly(I:C) was added at 100 μg/ml for 4 hrs before cell harvest. Negative control (NC) siRNA served as control. RNAiMAX transfection reagent was used in all the siRNA experiments. The upper panel shows the knockdown effects of siRNAs, & the lower panel shows representative Western blot images (presented from four independent experiments) & densitometry quantitative data (normalized into ‘fold of vehicle group’). All quantitative data are expressed as means ± S.D. aP < 0.05, AP < 0.01 versus vehicle; bP < 0.05, BP < 0.01 versus poly(I:C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-3102024168394.jpg)
![Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Western-Blot-NBP2-22114-img0002.jpg "Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)")
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lymphoid tissue tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0005.jpg)
![Flow Cytometry: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Flow cytometric analysis of A549 cells using LAP (TGF-beta 1) mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Flow-Cytometry-NBP2-22114-img0003.jpg)
![Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis of human stomach tissue (A) and human small intestine tissue (B) using TGF beta 1 antibody (NBP2-22114) at 2 ug/ml.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Western-Blot-NBP2-22114-img0007.jpg)
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Human tonsil. Image from verified customer review.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0006.jpg)
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0004.jpg)
![ELISA: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-ELISA-NBP2-22114-img0001.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - ACE-2 staining in immersion fixed paraffin-embedded sections of human lung normal (left) and lung cancer tissue (right) using Mouse Anti-Human ACE-2 (SN0754) Monoclonal Antibody (Catalog # NBP2-67692) at 10 ug/ml for 1 hour at room temperature followed by incubation with Goat anti-Mouse IgG Secondary Antibody.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunohistochemistry-Paraffin-NBP2-67692-img0009.jpg "Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - ACE-2 staining in immersion fixed paraffin-embedded sections of human lung normal (left) and lung cancer tissue (right) using Mouse Anti-Human ACE-2 (SN0754) Monoclonal Antibody (Catalog # NBP2-67692) at 10 ug/ml for 1 hour at room temperature followed by incubation with Goat anti-Mouse IgG Secondary Antibody.")
![Western Blot: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of ACE2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human kidney tissue lysateLane 2: human small intestine tissue lysate](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Western-Blot-NBP2-67692-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ACE-2 Antibody (SN0754) [NBP2-67692] - Staining ACE2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunocytochemistry-Immunofluorescence-NBP2-67692-img0003.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Rabbit Anti-Human ACE-2 (SN0754) Monoclonal Antibody (Catalog # NBP2-67692) at 3 ug/ml overnight at 4 degrees C. Detection kit: VC003. Counterstain: Hematoxylin. Localization: respiratory bronchioles.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunohistochemistry-Paraffin-NBP2-67692-img0010.jpg)
![Western Blot: ACE-2 Antibody (SN0754) [NBP2-67692] - Western blot analysis of ACE-2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE-2 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Western-Blot-NBP2-67692-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ACE-2 Antibody (SN0754) [NBP2-67692] - Staining ACE2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunocytochemistry-Immunofluorescence-NBP2-67692-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ACE-2 Antibody (SN0754) [NBP2-67692] - Staining ACE2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunocytochemistry-Immunofluorescence-NBP2-67692-img0002.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ACE2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunohistochemistry-Paraffin-NBP2-67692-img0004.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunohistochemistry-Paraffin-NBP2-67692-img0005.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of paraffin-embedded mouse kidney tissue using anti-ACE2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunohistochemistry-Paraffin-NBP2-67692-img0006.jpg)
![Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Western-Blot-NBP1-39474-img0003.jpg "Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0005.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Staining of human brain (putamen), antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0002.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-87 MG cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:100 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:400 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0008.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on hPBMCs with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control NBP2-14864. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0009.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Using the PE direct conjugate An intracellular stain was performed on SH-SY5Y cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control NB600-986PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin (PE).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0011.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0012.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin (PE).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0013.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin (APC).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0014.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on SH-SY5Y with Adenosine A2a R Antibody (7F6-G5-A2) NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0015.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0016.jpg)
