Growth and Development reagents: Novus Biologicals

NB300-223

Vimentin Antibody

(6 Reviews)

(83 Publications)

0.1 ml

NB300-223

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Human A7 cells. Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4C O/N followed with Alexa Fluor 488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Imaged with Leica TCS SP5 confocal microscope. ICC/IF image submitted by a verified customer review.

Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.molecularbrain.com/content/8/1/5), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep38221), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Analysis of HeLa cell culture. Antibody at 1:10,000 in green, and costained with mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - 10 um Cryosections from PFA fixed murine brain acute slices stained with Vimentin antibody (1:600) and Alexa Fluor 488 anti chicken IgG. Antibody showed specific staining, also of blood vessels. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - Infarcted mouse heart frozen sections stained with vimentin antibody at 1:200. Secondary antibody at 1:500. Fixation with 4% PFA. IHC-Fr image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. (A) Confocal microscopy images of dorsal & ventral regions of SVZ in G93A-SOD1 & Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), & vimentin (blue). Scale bar = 50 μm. (B) Quantification of nestin-, GFAP-, & vimentin-positive cells in SVZ of G93A-SOD1 & Wt-SOD1 mice. Data are means ± SD of 3 mice per group. **p 20 days (PyMT-1099 LT) were injected orthotopically into mammary fat pads of NSG mice. The graph represents tumor growth in PyMT-1099 & PyMT-1099 LT group of mice. (B) Histological tumor sections from tumors of PyMT-1099 or PyMT-1099 LT cells described in (A) were stained with H&E to assess the morphology of primary tumors. Representative microphotographs are shown from tumors of 2 out of the 6 mice used in the experiment. (C) Immunofluorescence analysis was performed to assess the expression of the EMT markers FN1, VIM, E-CAD & N-CAD in tumors formed by PyMT-1099 or PyMT-1099 LT cells in the experiment described in (A). DAPI was used as a nuclear counterstain. Representative pictures are shown from tumors of 2 out of the 6 mice used in the experiment. Scale bar, 100 μm. (D) The graph represents the number of lung metastases formed in NSG mice orthotopically transplanted with PyMT-1099 or PyMT-1099 LT cells; n = 6. (E) The graph represents the number of lung metastases formed in NSG mice injected with PyMT-1099 or PyMT-1099 LT cells through the tail vein; n = 6. The mice were sacrificed 8 weeks post-injection, & lungs were resected for the analysis of cancer cell colonization/ metastases formation. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30108334), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

DNM1P35 promotes EMT of ovarian cancer cells. (A,B) The expression of EMT markers were assessed by qPCR (A) and Western blotting (B) in SK-OV-3 and OVCAR-3 cells expressing shCTRL and shDNM1P35. (C,D) The expression of EMT markers were assessed by qPCR (C) and Western blotting (D) in SK-OV-3 and OVCAR-3 cells expressing vector and DNM1P35. (E,F) The expression of EMT markers were assessed by qPCR (E) and Western blotting (F) in SK-OV-3 and OVCAR-3 cells expressing shCTRL and shZEB1. (G,H) The expression of EMT markers were assessed by qPCR (G) and Western blotting (H) in SK-OV-3 and OVCAR-3 cells expressing shCTRL and shZEB1 and overexpression of DNM1P35. ***, p < 0.001, ****, p < 0.0001. ns, not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39732940), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

DNM1P35 targets miR-326 to promote ovarian cancer progression and EMT. (A) The potential target of DNM1P35 was predicted by the STAR (database. (B) Dual-luciferase activity assay in HEK293T cells expressing WT and MUT DNM1P35 luciferase reporter and treated with miR-NC or miR-326. (C) The expression of ZEB1 in SK-OV-3 and OVCAR-3 cells transfected with miR-NC and miR-326 was determined by qPCR. (D) The expression of EMT markers in SK-OV-3 and OVCAR-3 cells transfected with miR-NC and miR-326 was determined by Western blotting. (E) The growth of ovarian cancer cells was assessed by CCK-8 assay. (F) SK-OV-3 and OVCAR-3 cell colonies were stained by crystal violet. (G) Migration of SK-OV-3 and OVCAR-3 cells was measured by wound healing assay. (H) Invasion of SK-OV-3 and OVCAR-3 cells was measured by trans-well assay. ***, p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39732940), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq

Applications:

, ,

Applications: , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB100-77417

CD45 Antibody (30-F11) - BSA Free

(5 Reviews)

(48 Publications)

0.5 mg

NB100-77417

Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Mouse glomeruli stained with CD45 antibody. 4% formaldehyde fixed paraffin sections. EDTA was used for antigen retrieval. IHC-P image submitted by a verified customer review.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a FFPE embedded tissue section of mouse liver using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. The signal was developed using AEC reagent based method with hematoxylin counterstaining.

Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Staining of a FFPE mouse liver tissue section using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. Signal was detected using AF488 conjugated donkey anti-rat secondary antibody at 1:500 dilution. IHC-P image submitted by a verified customer review.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - VP1-positive infiltrating M2 macrophage and VP1-positive pneumocytes were detectable in the orally infected lung. Adjacent lung sections were examined with H&E and IHC staining for a pan-leukocyte marker CD45. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-77417AF350
Alexa Fluor 405	NB100-77417AF405
Alexa Fluor 488	NB100-77417AF488
Alexa Fluor 532	NB100-77417AF532
Alexa Fluor 594	NB100-77417AF594
Alexa Fluor 647	NB100-77417AF647
Alexa Fluor 700	NB100-77417AF700
Alexa Fluor 750	NB100-77417AF750
Alexa Fluor Plus 405	NB100-77417AFP405
Alexa Fluor Plus 488	NB100-77417AFP488
Alexa Fluor Plus 555	NB100-77417AFP555
Alexa Fluor Plus 594	NB100-77417AFP594
Alexa Fluor Plus 647	NB100-77417AFP647
Alexa Fluor Plus 680	NB100-77417AFP680
Allophycocyanin	NB100-77417APC
Biotin	NB100-77417B
CoraFluor 1	NB100-77417CL1
DyLight 350	NB100-77417UV
DyLight 405	NB100-77417V
DyLight 488	NB100-77417G
DyLight 550	NB100-77417R
DyLight 594	NB100-77417DL594
DyLight 650	NB100-77417C
DyLight 680	NB100-77417FR
DyLight 755	NB100-77417IR
FITC	NB100-77417F
HRP	NB100-77417H
Janelia Fluor 525	NB100-77417JF525
Janelia Fluor 549	NB100-77417JF549
Janelia Fluor 646	NB100-77417JF646
Janelia Fluor 669	NB100-77417JF669
PE	NB100-77417PE
PerCP	NB100-77417PCP
mFluor Violet 450 SE	NB100-77417MFV450
mFluor Violet 500 SE	NB100-77417MFV500
mFluor Violet 610 SE	NB100-77417MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80652-0.1mg
BSA Free	NB100-77417SS
Azide and BSA Free	NBP2-80652

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NBP2-22217

LAMP-2/CD107b Antibody (H4B4) - BSA Free

(2 Reviews)

(33 Publications)

0.1 ml

NBP2-22217

Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP-2/CD107b expression in Whole Cell Lysates: 2) HeLa, 3) Jurkat, 4) U937, 5) HepG2, Tissue Extracts: 6) Human kidney, 7) Human lung, 8) Human liver, and 9) Human placenta.

Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Staining of LAMP-2/CD107b in human liver using DAB with hematoxylin counterstain.

Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on U937 cells with HGF LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lane view shows a specific band for LAMP-2/CD107b in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

$Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Localization of TMEM135 to the mitochondria. The mitochondrial fraction isolated from the WT mouse brain show TMEM135 signals by immunoblotting. Following proteins were used as organelle markers: MFN2-mitochondria; LAMP2-lysosome; Lamin B1- nucleus; PDI- endoplasmic reticulum (ER). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19264), licensed under a CC-BY license.$

Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. Western blot analysis expression level of Cathepsin B (*P < 0.05) in total protein extract from RVA of I/R treated vs. control group. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphar.2018.01034/full), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LAMP-2/CD107b [NBP2-22217] at a 1:100 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using anti-LAMP-2/CD107b (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

ELISA: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Anti-LAMP-2/CD107b was used to assess lysates from human SVG-A cells via ELISA, by loading the indicated mass of total protein per well. Antibodies tested were diluted 1:250, and used in conjunction with an HRP-conjugated secondary antibody. Signal was detected by luminescence. Image from verified customer review.

LAMP-2/CD107b (H4B4) was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Mouse anti-LAMP-2/CD107b (H4B4) Protein G-purified Monoclonal Antibody (Catalog # NBP2-22217) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Mouse IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

LAMP-2/CD107b (H4B4) was detected in immersion fixed MCF7 human breast cancer cell line using Mouse anti-LAMP-2/CD107b (H4B4) Protein G-purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-22217AF647) (light blue) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Ch, Rt(-)

Species Glossary

Reactivity: Hu, Mu, Ch, Rt(-)

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22217AF350
Alexa Fluor 405	NBP2-22217AF405
Alexa Fluor 488	NBP2-22217AF488
Alexa Fluor 532	NBP2-22217AF532
Alexa Fluor 594	NBP2-22217AF594
Alexa Fluor 647	NBP2-22217AF647
Alexa Fluor 700	NBP2-22217AF700
Alexa Fluor 750	NBP2-22217AF750
Alexa Fluor Plus 405	NBP2-22217AFP405
Alexa Fluor Plus 488	NBP2-22217AFP488
Alexa Fluor Plus 555	NBP2-22217AFP555
Alexa Fluor Plus 594	NBP2-22217AFP594
Alexa Fluor Plus 647	NBP2-22217AFP647
Alexa Fluor Plus 680	NBP2-22217AFP680
Allophycocyanin	NBP2-21799
Allophycocyanin	NBP2-22217APC
Allophycocyanin/Cy7	NBP2-22217APCCY7
Biotin	NBP2-22217B
CoraFluor 1	NBP2-22217CL1
DyLight 350	NBP2-22217UV
DyLight 405	NBP2-22217V
DyLight 488	NBP2-22217G
DyLight 550	NBP2-22217R
DyLight 594	NBP2-22217DL594
DyLight 650	NBP2-22217C
DyLight 680	NBP2-22217FR
DyLight 755	NBP2-22217IR
FITC	NBP2-22217F
FITC	NBP1-28553
HRP	NBP2-22217H
Janelia Fluor 525	NBP2-22217JF525
Janelia Fluor 549	NBP2-22217JF549
Janelia Fluor 646	NBP2-22217JF646
Janelia Fluor 669	NBP2-22217JF669
PE	NBP2-22217PE
PE/Atto594	NBP2-22217PEATT594
PE/Cy5.5	NBP2-22217PECY55
PE/Cy7	NBP2-22217PECY7
PerCP	NBP2-22217PCP
mFluor Violet 450 SE	NBP2-22217MFV450
mFluor Violet 500 SE	NBP2-22217MFV500
mFluor Violet 610 SE	NBP2-22217MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22217SS
Azide and BSA Free	NBP2-80825

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NBP2-31376

FoxO1/FKHR Antibody (83N7F8) - BSA Free

(1 Review)

(7 Publications)

0.1 mg

NBP2-31376

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt, Po, Bv, Sq

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Sq

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-31376AF350
Alexa Fluor 405	NBP2-31376AF405
Alexa Fluor 488	NBP2-31376AF488
Alexa Fluor 532	NBP2-31376AF532
Alexa Fluor 594	NBP2-31376AF594
Alexa Fluor 647	NBP2-31376AF647
Alexa Fluor 700	NBP2-31376AF700
Alexa Fluor 750	NBP2-31376AF750
Alexa Fluor Plus 405	NBP2-31376AFP405
Alexa Fluor Plus 488	NBP2-31376AFP488
Alexa Fluor Plus 555	NBP2-31376AFP555
Alexa Fluor Plus 594	NBP2-31376AFP594
Alexa Fluor Plus 647	NBP2-31376AFP647
Alexa Fluor Plus 680	NBP2-31376AFP680
Allophycocyanin	NBP2-31376APC
Biotin	NBP2-31376B
CoraFluor 1	NBP2-31376CL1
DyLight 350	NBP2-31376UV
DyLight 405	NBP2-31376V
DyLight 488	NBP2-31376G
DyLight 550	NBP2-31376R
DyLight 594	NBP2-31376DL594
DyLight 650	NBP2-31376C
DyLight 680	NBP2-31376FR
DyLight 755	NBP2-31376IR
FITC	NBP2-31376F
HRP	NBP2-31376H
Janelia Fluor 525	NBP2-31376JF525
Janelia Fluor 549	NBP2-31376JF549
Janelia Fluor 646	NBP2-31376JF646
Janelia Fluor 669	NBP2-31376JF669
PE	NBP2-31376PE
PerCP	NBP2-31376PCP
mFluor Violet 450 SE	NBP2-31376MFV450
mFluor Violet 500 SE	NBP2-31376MFV500
mFluor Violet 610 SE	NBP2-31376MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-31376SS
Azide and BSA Free	NBP2-80741

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NB500-644

Survivin Antibody (8E2) - BSA Free

(15 Publications)

0.1 ml

NB500-644

Flow (Intracellular): Survivin Antibody (8E2) [NB500-644] - An intracellular stain was performed on Daudi cells with Survivin Antibody (8E2) [NB500-644] (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

An intracellular stain was performed on U-2 OS human osteosarcoma cell line with Mouse anti-Survivin (8E2) Protein-G purified Monoclonal Antibody conjugated to Phycoerythrin (Catalog # NB500-644PE, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-644AF350
Alexa Fluor 405	NB500-644AF405
Alexa Fluor 488	NB500-644AF488
Alexa Fluor 532	NB500-644AF532
Alexa Fluor 594	NB500-644AF594
Alexa Fluor 647	NB500-644AF647
Alexa Fluor 700	NB500-644AF700
Alexa Fluor 750	NB500-644AF750
Alexa Fluor Plus 405	NB500-644AFP405
Alexa Fluor Plus 488	NB500-644AFP488
Alexa Fluor Plus 555	NB500-644AFP555
Alexa Fluor Plus 594	NB500-644AFP594
Alexa Fluor Plus 647	NB500-644AFP647
Alexa Fluor Plus 680	NB500-644AFP680
Allophycocyanin	NB500-644APC
Biotin	NB500-644B
CoraFluor 1	NB500-644CL1
DyLight 350	NB500-644UV
DyLight 405	NB500-644V
DyLight 488	NB500-644G
DyLight 550	NB500-644R
DyLight 594	NB500-644DL594
DyLight 650	NB500-644C
DyLight 680	NB500-644FR
DyLight 755	NB500-644IR
FITC	NB500-644F
HRP	NB500-644H
Janelia Fluor 525	NB500-644JF525
Janelia Fluor 549	NB500-644JF549
Janelia Fluor 646	NB500-644JF646
Janelia Fluor 669	NB500-644JF669
PE	NB500-644PE
PerCP	NB500-644PCP
mFluor Violet 450 SE	NB500-644MFV450
mFluor Violet 500 SE	NB500-644MFV500
mFluor Violet 610 SE	NB500-644MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB500-644SS
Azide and BSA Free	NBP2-80970

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NB300-228

SCP1 Antibody - BSA Free

(13 Publications)

0.1 ml

NB300-228

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-228AF350
Alexa Fluor 405	NB300-228AF405
Alexa Fluor 488	NB300-228AF488
Alexa Fluor 532	NB300-228AF532
Alexa Fluor 594	NB300-228AF594
Alexa Fluor 647	NB300-228AF647
Alexa Fluor 700	NB300-228AF700
Alexa Fluor 750	NB300-228AF750
Alexa Fluor Plus 405	NB300-228AFP405
Alexa Fluor Plus 488	NB300-228AFP488
Alexa Fluor Plus 555	NB300-228AFP555
Alexa Fluor Plus 594	NB300-228AFP594
Alexa Fluor Plus 647	NB300-228AFP647
Alexa Fluor Plus 680	NB300-228AFP680
Allophycocyanin	NB300-228APC
Biotin	NB300-228B
CoraFluor 1	NB300-228CL1
DyLight 350	NB300-228UV
DyLight 405	NB300-228V
DyLight 488	NB300-228G
DyLight 550	NB300-228R
DyLight 594	NB300-228DL594
DyLight 650	NB300-228C
DyLight 680	NB300-228FR
DyLight 755	NB300-228IR
FITC	NB300-228F
HRP	NB300-228H
Janelia Fluor 525	NB300-228JF525
Janelia Fluor 549	NB300-228JF549
Janelia Fluor 646	NB300-228JF646
Janelia Fluor 669	NB300-228JF669
PE	NB300-228PE
PerCP	NB300-228PCP
mFluor Violet 450 SE	NB300-228MFV450
mFluor Violet 500 SE	NB300-228MFV500
mFluor Violet 610 SE	NB300-228MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-228SS

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NB100-481

BP1 Antibody - BSA Free

(1 Review)

(7 Publications)

0.1 ml

NB100-481

Western Blot: BP1 Antibody [NB100-481] - Effect of BP1 on TNFalpha-induced cell death. Western blot analysis of Beta Protein 1 (BP1) protein expression in MCF7/EV cell lines and MCF7/BP1 cell lines. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1766), licensed under a CC-BY license.

Immunoprecipitation: BP1 Antibody [NB100-481] - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 (NB100-481) antibody diluted 1:1,000 3. Negative control for IP in lane 2: cell extract incubated with 50ul of Protein A/G beads.

Effect of BP1 on TNF alpha -induced cell death. (a) Western blot analysis of Beta Protein 1 (BP1) protein expression in MCF7/EV cell lines and MCF7/BP1 cell lines.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-481AF350
Alexa Fluor 405	NB100-481AF405
Alexa Fluor 488	NB100-481AF488
Alexa Fluor 532	NB100-481AF532
Alexa Fluor 594	NB100-481AF594
Alexa Fluor 647	NB100-481AF647
Alexa Fluor 700	NB100-481AF700
Alexa Fluor 750	NB100-481AF750
Alexa Fluor Plus 405	NB100-481AFP405
Alexa Fluor Plus 488	NB100-481AFP488
Alexa Fluor Plus 555	NB100-481AFP555
Alexa Fluor Plus 594	NB100-481AFP594
Alexa Fluor Plus 647	NB100-481AFP647
Alexa Fluor Plus 680	NB100-481AFP680
Allophycocyanin	NB100-481APC
Biotin	NB100-481B
CoraFluor 1	NB100-481CL1
DyLight 350	NB100-481UV
DyLight 405	NB100-481V
DyLight 488	NB100-481G
DyLight 550	NB100-481R
DyLight 594	NB100-481DL594
DyLight 650	NB100-481C
DyLight 680	NB100-481FR
DyLight 755	NB100-481IR
FITC	NB100-481F
HRP	NB100-481H
Janelia Fluor 525	NB100-481JF525
Janelia Fluor 549	NB100-481JF549
Janelia Fluor 646	NB100-481JF646
Janelia Fluor 669	NB100-481JF669
PE	NB100-481PE
PerCP	NB100-481PCP
mFluor Violet 450 SE	NB100-481MFV450
mFluor Violet 500 SE	NB100-481MFV500
mFluor Violet 610 SE	NB100-481MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-481SS

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NB100-56167

Survivin Antibody - BSA Free

(6 Publications)

0.05 ml

NB100-56167

Immunohistochemistry: Survivin Antibody [NB100-56167] - Immunohistochemical analysis of Survivin in formalin-fixed paraffin-embedded human prostate and breast cancer using Survivin Antibody [NB100-56167]. A and A1, prostate cancer at low and high magnification, respectively. B and B1, breast cancer. B1 is a high magnification of an area of metastasis. Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56167] - Analysis of Survivin in a formalin-fixed paraffin-embedded human brain tissue microarray using Survivin Antibody [NB100-56167]. A. Differential expression of Survivin in brain tumors from six different patients. A1-A3, high magnification from A. A1, gemistocytic astrocytoma. A2, medulloblastoma. A3, choroid plexus papilloma. Hematoxylin-eosin counterstain.

Survivin was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-Survivin Whole Serum (Catalog # NB100-56167) at a 1:500 dilution overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Survivin was detected in immersion fixed CHO-K1 Chinese hamster ovary epithelium cell line using Rabbit anti-Survivin Whole Serum (Catalog # NB100-56167) at a 1:500 dilution overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Representative Western blots showing the IAP levels in EV derived from individual European American (EA) (N1-N5) & (N7-N9), *N6 and African American (AA) (N1-N9) patients with prostate cancer (PCa).Specific antibodies against Survivin, XIAP, cIAP-1, cIAP-2, and Lamp1 were used for the Western blotting analysis of total exosomal proteins. The blots from both patient groups were processed under identical conditions; Lamp 1 was used as loading control. (*N6, Hispanic.) (Both blots were done side by side in the same gel running and transferring apparatus, blocking, washing buffers, and antibody incubations were done in the same time, in the same incubating trays under the identical exposure to keep the consistencies.) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28981528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ma, Po, Bv, Ca, Fe, Pm-Or

Species Glossary

Reactivity: Hu, Mu, Rt, Ma, Po, Bv, Ca, Fe, Pm-Or

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5754

Alt Catalog# IMG-5754

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56167SS

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NBP1-78292

Notch-1 Antibody - BSA Free

(1 Review)

(5 Publications)

0.1 ml

NBP1-78292

Western Blot: Notch-1 Antibody [NBP1-78292] - Analysis of Cleaved Notch1 in Jurkat cell lysate.

Simple Western: Notch-1 Antibody [NBP1-78292] - Lane view shows a specific band for Notch-1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-78292AF350
Alexa Fluor 405	NBP1-78292AF405
Alexa Fluor 488	NBP1-78292AF488
Alexa Fluor 532	NBP1-78292AF532
Alexa Fluor 594	NBP1-78292AF594
Alexa Fluor 647	NBP1-78292AF647
Alexa Fluor 700	NBP1-78292AF700
Alexa Fluor 750	NBP1-78292AF750
Alexa Fluor Plus 405	NBP1-78292AFP405
Alexa Fluor Plus 488	NBP1-78292AFP488
Alexa Fluor Plus 555	NBP1-78292AFP555
Alexa Fluor Plus 594	NBP1-78292AFP594
Alexa Fluor Plus 647	NBP1-78292AFP647
Alexa Fluor Plus 680	NBP1-78292AFP680
Allophycocyanin	NBP1-78292APC
Biotin	NBP1-78292B
CoraFluor 1	NBP1-78292CL1
DyLight 350	NBP1-78292UV
DyLight 405	NBP1-78292V
DyLight 488	NBP1-78292G
DyLight 550	NBP1-78292R
DyLight 594	NBP1-78292DL594
DyLight 650	NBP1-78292C
DyLight 680	NBP1-78292FR
DyLight 755	NBP1-78292IR
FITC	NBP1-78292F
HRP	NBP1-78292H
Janelia Fluor 525	NBP1-78292JF525
Janelia Fluor 549	NBP1-78292JF549
Janelia Fluor 646	NBP1-78292JF646
Janelia Fluor 669	NBP1-78292JF669
PE	NBP1-78292PE
PerCP	NBP1-78292PCP
mFluor Violet 450 SE	NBP1-78292MFV450
mFluor Violet 500 SE	NBP1-78292MFV500
mFluor Violet 610 SE	NBP1-78292MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-78292SS

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NB100-1830

Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free

(6 Publications)

0.1 ml

NB100-1830

Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid

Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.

Reactivity:

Hu, Mu, Rt, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Rb

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-1830AF350
Alexa Fluor 405	NB100-1830AF405
Alexa Fluor 488	NB100-1830AF488
Alexa Fluor 532	NB100-1830AF532
Alexa Fluor 594	NB100-1830AF594
Alexa Fluor 647	NB100-1830AF647
Alexa Fluor 700	NB100-1830AF700
Alexa Fluor 750	NB100-1830AF750
Alexa Fluor Plus 488	NB100-1830AFP488
Alexa Fluor Plus 555	NB100-1830AFP555
Alexa Fluor Plus 594	NB100-1830AFP594
Alexa Fluor Plus 647	NB100-1830AFP647
Alexa Fluor Plus 680	NB100-1830AFP680
Allophycocyanin	NB100-1830APC
Biotin	NB100-1830B
CoraFluor 1	NB100-1830CL1
DyLight 350	NB100-1830UV
DyLight 405	NB100-1830V
DyLight 488	NB100-1830G
DyLight 550	NB100-1830R
DyLight 594	NB100-1830DL594
DyLight 650	NB100-1830C
DyLight 680	NB100-1830FR
DyLight 755	NB100-1830IR
FITC	NB100-1830F
HRP	NB100-1830H
Janelia Fluor 525	NB100-1830JF525
Janelia Fluor 549	NB100-1830JF549
Janelia Fluor 646	NB100-1830JF646
Janelia Fluor 669	NB100-1830JF669
PE	NB100-1830PE
PerCP	NB100-1830PCP
mFluor Violet 450 SE	NB100-1830MFV450
mFluor Violet 500 SE	NB100-1830MFV500
mFluor Violet 610 SE	NB100-1830MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80607
BSA Free	NB100-1830SS

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NBP2-50599

EGFR Antibody (DH8.3) - EGFRvIII, Mutant - BSA Free

(12 Publications)

0.1 ml

NBP2-50599

Flow Cytometry: Mouse Monoclonal EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - Analysis of glioblastoma cell lines using EGFR Antibody. Image from a verified customer review.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-50599AF350
Alexa Fluor 405	NBP2-50599AF405
Alexa Fluor 488	NBP2-50599AF488
Alexa Fluor 532	NBP2-50599AF532
Alexa Fluor 594	NBP2-50599AF594
Alexa Fluor 647	NBP2-50599AF647
Alexa Fluor 700	NBP2-50599AF700
Alexa Fluor 750	NBP2-50599AF750
Alexa Fluor Plus 405	NBP2-50599AFP405
Alexa Fluor Plus 488	NBP2-50599AFP488
Alexa Fluor Plus 555	NBP2-50599AFP555
Alexa Fluor Plus 594	NBP2-50599AFP594
Alexa Fluor Plus 647	NBP2-50599AFP647
Alexa Fluor Plus 680	NBP2-50599AFP680
Allophycocyanin	NBP2-50599APC
Allophycocyanin/Cy7	NBP2-50599APCCY7
Biotin	NBP2-50599B
CoraFluor 1	NBP2-50599CL1
DyLight 350	NBP2-50599UV
DyLight 405	NBP2-50599V
DyLight 488	NBP2-50599G
DyLight 550	NBP2-50599R
DyLight 594	NBP2-50599DL594
DyLight 650	NBP2-50599C
DyLight 680	NBP2-50599FR
DyLight 755	NBP2-50599IR
FITC	NBP2-50599F
HRP	NBP2-50599H
Janelia Fluor 525	NBP2-50599JF525
Janelia Fluor 549	NBP2-50599JF549
Janelia Fluor 646	NBP2-50599JF646
Janelia Fluor 669	NBP2-50599JF669
PE	NBP2-50599PE
PE/Atto594	NBP2-50599PEATT594
PE/Cy5.5	NBP2-50599PECY55
PE/Cy7	NBP2-50599PECY7
PerCP	NBP2-50599PCP
mFluor Violet 450 SE	NBP2-50599MFV450
mFluor Violet 500 SE	NBP2-50599MFV500
mFluor Violet 610 SE	NBP2-50599MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-50599-0.025ml

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NB100-407

14-3-3 gamma Antibody (HS23) - BSA Free

(11 Publications)

0.1 ml

NB100-407

Reactivity:

Hu, Mu, Rt, Bv, Ch, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ch, Ze

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-407SS

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Vimentin Antibody

CD45 Antibody (30-F11) - BSA Free

LAMP-2/CD107b Antibody (H4B4) - BSA Free

FoxO1/FKHR Antibody (83N7F8) - BSA Free

Survivin Antibody (8E2) - BSA Free

SCP1 Antibody - BSA Free

BP1 Antibody - BSA Free

Survivin Antibody - BSA Free

Notch-1 Antibody - BSA Free

Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free

EGFR Antibody (DH8.3) - EGFRvIII, Mutant - BSA Free

14-3-3 gamma Antibody (HS23) - BSA Free