GAP-43 Antibody [Biotin]

Immunohistochemistry-Paraffin: GAP-43 Antibody [Biotin] [NB300-143B] - Representative images of hematoxylin and eosin. Differences in the number of OMP+ mature olfactory receptor neurons (ORNs). Image collected and cropped by Citeab from the following publication (Reduction of Proliferating Olfactory Cells and Low Expression of Extracellular Matrix Genes Are Hallmarks of the Aged Olfactory Mucosa. Front Aging Neurosci (2018)) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: GAP-43 Antibody [Biotin] [NB300-143B] - Immunohistochemistry-Paraffin: GAP-43 Antibody [Biotin] [NB300-143B] - Representative images of immunohistological staining (brown) of OMP-positive (OMP+) cells, GAP43+ immature ORNs. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Numbers of SOX2+ ORN progenitors and Ki67+ actively proliferating cells per mm of the basal layer and OMP+ mature ORNs, GAP43+ immature ORNs, and Cas3+ apoptotic cells per mm of the OE in saline or rhIGF-1-treated mice. Open circles, rectangles, and triangles represent the values for each mouse in the saline, low-IGF-1, and high-IGF-1 treated groups (each n = 6), respectively. Image collected and cropped by Citeab from the following publication (Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium. Front Aging Neurosci (2018)) licensed under a CC-BY license.

Immunohistochemistry: GAP-43 Antibody [Biotin] [NB300-143B] - (A) Representative images of immunohistological staining (brown) of SOX2+ olfactory receptor neurons (ORN) progenitor cells, Gap43+ immature ORNs, Ki67+ proliferating cells & cleaved caspase-3+ (Cas3+) apoptotic cells. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Arrowheads indicate Cas3+ apoptotic cells in the OE (n = 6). (B) Numbers of SOX2+ ORN progenitors & Ki67+ actively proliferating cells per mm of the basal layer, & Gap43+ immature ORNs & Cas3+ apoptotic cells per mm of the OE in saline or CSS-treated mice. Data represent means ± SEM (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001 compared with saline-treated mice (one-way ANOVA). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29950987), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Eq, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Biotin

GAP-43 Antibody [Biotin] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH.
• Localization: Neuronal Marker, developing and regenerating axons and growth cone.
• Marker: Neuronal Marker
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GAP43
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This GAP43 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded and frozen sections and Western blot, where it recognizes a band at 43 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Reactivity to Canine, Chicken, and Primate reported in scientific literature (PMID: 30647968, 30819546, and 24249398 respectively). Drosophila reactivity reported in scientific literature (PMID: 30819546).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GAP-43 Antibody [Biotin]

• Axonal membrane protein GAP-43
• B-50
• Basp2
• calmodulin-binding protein P-57
• GAP43
• GAP-43
• growth associated protein 43
• Growth-associated protein 43
• nerve growth-related peptide GAP43
• Neural phosphoprotein B-50
• Neuromodulin
• neuron growth-associated protein 43
• PP46
• protein F1

Background

GAP-43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAP-43 Antibody (NB300-143B)(8)

Publications using NB300-143B

• Mori E, Ueha R, Tanaka H et al. Elezanumab Accelerates Post-Injury Regeneration of Olfactory Sensory Neurons in a Methimazole-Induced Mouse Model. International forum of allergy & rhinology 2025-08-04 [PMID: 40879266]
• Ueha R, Ito T, Ueha S et al. Evidence for the spread of SARS-CoV-2 and olfactory cell lineage impairment in close-contact infection Syrian hamster models Frontiers in Cellular and Infection Microbiology 2022-10-21 [PMID: 36339331]
• Ueha R, Ito T, Furukawa R et al. Oral SARS-CoV-2 Inoculation Causes Nasal Viral Infection Leading to Olfactory Bulb Infection: An Experimental Study Frontiers in Cellular and Infection Microbiology 2022-06-13 [PMID: 35770069]
• Mori E, Ueha R, Kondo K et al. Squamous and Respiratory Metaplasia After Olfactory Mucosal Resection Frontiers in Neuroscience 2021-07-20 [PMID: 34354563]
• Ueha R, Kondo K, Ueha S, Yamasoba T. Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium Front Aging Neurosci 2018-11-20 [PMID: 30515092]
• Ueha R, Ueha S, Kondo K et al. Cigarette Smoke-Induced Cell Death Causes Persistent Olfactory Dysfunction in Aged Mice Front Aging Neurosci 2018-06-13 [PMID: 29950987]
• Ueha R, Shichino S, Ueha S et al. Reduction of Proliferating Olfactory Cells and Low Expression of Extracellular Matrix Genes Are Hallmarks of the Aged Olfactory Mucosa. Front Aging Neurosci 2018-03-27 [PMID: 29636678] (Mouse)
• Ueha R, Ueha S, Sakamoto T et al. Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice. Neurotox Res 2016-08-01 [PMID: 27003941]

Bioinformatics

• Gene Symbol: GAP43