Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free [NBP2-80956] - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein. Image using the standard format of this product.

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free [NBP2-80956] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.

Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free [NBP2-80956] - Staining of human enodmetrial glands within the uterus using NB300-146. Note the absence of staining in the surrounding myometrial smooth muscle. Image using the standard format of this product.

Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free [NBP2-80956] - An intracellular stain was performed on A549 cells with - Purified NBP2-61137 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image using the Purified form of this antibody.

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free [NBP2-80956] - Detection of ATPA1 (Green) in HepG2 cells using NB300-146. Nuclei (Blue) were counterstained using Hoechst 33258. Image using the standard format of this product.

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free [NBP2-80956] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.

Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free [NBP2-80956] - An intracellular stain was performed on A549 cells with NBP2-61137AF647 (Blue) and a matched isotype control (Orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Dr, Gp, Pm, Rb, Sh, Xp, Ye
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: 464.6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free (NB300-146) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified Sodium Potassium ATPase Alpha 1 from rabbit renal outer medulla. [UniProt# Q9N0Z6]
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP1A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 26941236)
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation reported in scientific literature (PMID 27748972)
  • Western Blot 1:1000-1:10000
• Application Notes: In Western Blot, a distinct band at ~ 112 kDa is seen. Do not boil the sample prior to loading on the gel for Western Blot (60 degrees Celsius appears to work fine).
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Publication with sheep shown (PMID: 18424241); however results may vary.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Azide and BSA Free

• ATP1A1
• ATPase, Na+/K+ transporting, alpha 1 polypeptide
• EC 3.6.3
• EC 3.6.3.9
• EC 7.2.2.13
• K-ATPase alpha-1 subunit
• K-ATPase catalytic subunit alpha-A protein
• MGC3285
• MGC51750
• Na
• Na(+)/K(+) ATPase alpha-1 subunit
• Na, K-ATPase, alpha-A catalytic polypeptide
• Na+, K+ ATPase alpha subunit
• Na+/K+ ATPase 1
• Sodium Potassium ATPase Alpha 1
• sodium pump 1
• Sodium pump subunit alpha-1
• sodium/potassium-transporting ATPase subunit alpha-1
• sodium-potassium-ATPase, alpha 1 polypeptide

Background

Sodium Potassium ATPase alpha 1 (ATP1A1) belongs to the P-type ATPase family and is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with exchange of sodium (Na+) and potassium (K+ ) ions across the cellular plasma membranes. This action creates an electrochemical gradient of Na+ and K+ ions, providing the energy for active transport of various nutrients. Its phosphorylation at Tyr-10 position alters the pumping activity whereas dephosphorylation mediated by PP2A (protein phosphatase 2A) following increases in intracellular Na+, leads to catalytic activity. Because the plasma membrane is highly permeable to water, it is the concentration of ions across this membrane that is critical for maintaining an adequate cell volume and sodium potassium ATPase is key player in this maintainance process because it provides the driving force for active Na+ as well as K+ transport into/out of the cell, with water following isosmotically. This pump is consists of equimolar ratios of two main subunits, the catalytic alpha subunit (which contains the binding sites for the mentioned cations, ATP, and cardiotonic steroid inhibitors) and the regulatory beta polypeptides (which is essential for the normal activity of the enzyme as well as involved potentially in the occlusion of K+ and modulation of the enzyme's K+ /Na+ affinity).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Sodium Potassium ATPase Alpha 1 Antibody (NBP2-80956). (Showing 1 - 1 of 1 FAQ).

1. I am looking forward to pick up a plasma membrane marker. I have gastric cancer cell line and I am preparing PM out of that. To just check the quality of prep, I need a plasma membrane marker.
   • Our sodium potassium ATPase and plasma membrane products may be of use to you. Please contact us at technical@novusbio.com with any additional questions.

Bioinformatics

• Gene Symbol: ATP1A1