SCP1 Antibody - BSA Free

Images

 
Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing ...read more
Western Blot: SCP1 Antibody [NB300-229] - Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 ...read more
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Double immunofluorescence of testicular spread preparations of the adult mice, SYCP3 (green) and SCP1 (red). Image collected and cropped by ...read more
Immunohistochemistry-Paraffin: SCP1 Antibody [NB300-229] - Punctate staining of murine SCP1 in mouse ovary using NB300-229.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - SCP1 labeled in mouse pachytene preparation (red), using NB300-229. CDK2 staining, near teleomeres, is also present (green).
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - Spermatocytes cells fixed in PFA. Detected with anti-mouse 594. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatozoa. Green: SCP1 staining. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatocyte. Red: scp1 colocalized with scp3 (green). ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale ...read more

Product Details

Summary
Reactivity Mu, Rt, Ch, Ma, Pa, Pm, Hu(-)Species Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

SCP1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to the C-terminus of the mouse SCP1 protein sequence. [UniProt# Q62209]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYCP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:750
  • Immunohistochemistry 1:750
  • Immunohistochemistry-Frozen 1:750
  • Immunohistochemistry-Paraffin 1:750
  • Immunoprecipitation
  • Simple Western 1:100
  • Western Blot
Application Notes
This SCP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 27486799). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
SCP1 Peptide (NB300-229PEP)
Reviewed Applications
Read 3 Reviews rated 5
using
NB300-229 in the following applications:

Publications
Read Publications using
NB300-229 in the following applications:

  • 1 publication
  • 23 publications
  • IHC
    8 publications
  • 2 publications
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 25981592). Parasite reactivity reported in scientific literature (PMID: 27084479). Chicken reactivity reported in scientific literature (PMID: 28174243). Use in Monkey reported in scientific literature (PMID: 31907447). This antibody has not been shown to have human reactivity.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SCP1 Antibody - BSA Free

  • Cancer/testis antigen 8
  • CT8MGC104417
  • HOM-TES-14
  • SCP1SCP-1
  • synaptonemal complex protein 1

Background

Synaptonemal complex protein 1 is a major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. SCP1 is equired for normal assembly of the central element of the synaptonemal complexes, as well as for normal centromere pairing during meiosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-229
Species: Mu, Rt, Ch, Ma, Pa, Pm, Hu(-)
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for SCP1 Antibody (NB300-229)(49)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Mammal, Monkey, Parasite.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
ICC/IF
(23)
IHC
(8)
IHC-P
(2)
IP
(1)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(35)
Rat
(1)
Mammal
(2)
Monkey
(1)
Parasite
(1)
All Species
Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using NB300-229 Applications Species
Lee S, Kuramochi-Miyagawa S, Nagamori I, Nakano T Effects of transgene insertion loci and copy number on Dnmt3L gene silencing through antisense transgene-derived PIWI-interacting RNAs RNA (New York, N.Y.) Feb 10 2022 [PMID: 35145000] (ICC/IF, Mouse) ICC/IF Mouse
Kagiwada S, Aramaki S, Wu G et al. YAP creates epiblast responsiveness to inductive signals for germ cell fate Development (Cambridge, England) Sep 16 2021 [PMID: 34528691] (ICC/IF, Mouse) ICC/IF Mouse
Takada Y, Yaman-Deveci R, Shirakawa T et al. Maintenance DNA methylation in pre-meiotic germ cells regulates meiotic prophase by facilitating homologous chromosome pairing Development (Cambridge, England) May 15 2021 [PMID: 33998651]
Osawa Y, Murata K, Usui M et al. EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice eLife May 11 2021 [PMID: 33973520] (IHC-P, Mouse) IHC-P Mouse
Spangenberg V, Arakelyan M, Cioffi MB et al. Cytogenetic mechanisms of unisexuality in rock lizards Sci Rep May 26 2020 [PMID: 32457493] (ICC/IF) ICC/IF
Hamada N, Hamazaki N, Shimamoto S et Al. Germ cell-intrinsic effects of sex chromosomes on early oocyte differentiation in mice PLoS Genet. Mar 26 2020 [PMID: 32214314] (IHC, Mouse) IHC Mouse
Zhang B, Tang Z, Li L, Lu LY NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis Cell Death Differ. Jan 21 2020 [PMID: 31965061] (ICC/IF, Mouse) ICC/IF Mouse
Carbajal A, Gryniuk I, de Castro R, Pezza R Efficient enrichment of synchronized mouse spermatocytes suitable for genome-wide analysis. bioRxiv Jan 1 2022 (Mouse) Mouse
Zhang Y, Schroeder LK, Lessard MD et al. Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging Nat. Methods Jan 6 2020 [PMID: 31907447] (ICC/IF, Mouse, Human, Monkey) ICC/IF Mouse, Human, Monkey
Hua R, Wei H, Liu C et al. FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis Nucleic Acids Res. Nov 14 2019 [PMID: 31724724] (ICC/IF, Mouse) ICC/IF Mouse
Show All 49 Publications.

Reviews for SCP1 Antibody (NB300-229) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB300-229:
Filter by Applications
Immunocytochemistry
(3)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SCP1 NB300-229
Enlarge
5
reviewed by:
xiaoyu zhang
Immunocytochemistry Mouse 02/17/2021
View

Summary

ApplicationImmunocytochemistry
Sample Testedspermatocyte
SpeciesMouse
LotE3
Immunocytochemistry SCP1 NB300-229
Enlarge
5
reviewed by:
Nin Wan
Immunocytochemistry Mouse 09/12/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse spermatozoa
SpeciesMouse
LotD2
Immunocytochemistry SCP1 NB300-229
Enlarge
5
reviewed by:
Verified Customer
Immunocytochemistry Mouse 04/11/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedSpermatocytes
SpeciesMouse
LotC-2

Comments

CommentsSpermatocytes cells fixed in PFA. Detected with anti-mouse 594

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for SCP1 Antibody (NB300-229) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Array NB300-229

Bioinformatics Tool for SCP1 Antibody (NB300-229)

Discover related pathways, diseases and genes to SCP1 Antibody (NB300-229). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for SCP1 Antibody (NB300-229)

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Blogs on SCP1.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

SCP1 a Potential Cancer Target for Immunotherapy
Synaptonemal Complex Protein 1 (SCP1) is a novel tumor antigen that belongs to the growing family of cancer/testis antigens (CTA). SCP-1 is known to be selectively expressed during the meiotic prophase of spermatocytes and is involved in the pairing o...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

xiaoyu zhang
02/17/2021
Application: Immunocytochemistry
Species: Mouse

Nin Wan
09/12/2019
Application: Immunocytochemistry
Species: Mouse

Verified Customer
04/11/2017
Application: Immunocytochemistry
Species: Mouse

Bioinformatics

Gene Symbol SYCP1