SCP1 Antibody

Images

 
Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing ...read more
Western Blot: SCP1 Antibody [NB300-229] - Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 ...read more
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatozoa. Green: SCP1 staining. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SCP1 Antibody [NB300-229] - Punctate staining of murine SCP1 in mouse ovary using NB300-229.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - SCP1 labeled in mouse pachytene preparation (red), using NB300-229. CDK2 staining, near teleomeres, is also present (green).
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - Spermatocytes cells fixed in PFA. Detected with anti-mouse 594. ICC/IF image submitted by a verified customer review.

Product Details

Summary
Reactivity Mu, Rt, Ch, Ma, Pa, Pm, Hu(-)Species Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SCP1 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminus of the mouse SCP1 protein sequence. [UniProt# Q62209]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYCP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western 1:100
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:750
  • Immunohistochemistry 1:750
  • Immunohistochemistry-Frozen 1:750
  • Immunohistochemistry-Paraffin 1:750
  • Immunoprecipitation
Application Notes
This SCP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID 27486799). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
SCP1 Peptide (NB300-229PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NB300-229 in the following applications:

Publications
Read Publications using
NB300-229 in the following applications:

  • 1 publication
  • 21 publications
  • IHC
    8 publications
  • 1 publication
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 25981592). Parasite reactivity reported in scientific literature (PMID: 27084479). Chicken reactivity reported in scientific literature (PMID: 28174243). Use in Monkey reported in scientific literature (PMID:31907447). This antibody has not been shown to have human reactivity.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SCP1 Antibody

  • Cancer/testis antigen 8
  • CT8MGC104417
  • HOM-TES-14
  • SCP1SCP-1
  • synaptonemal complex protein 1

Background

Synaptonemal complex protein 1 is a major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. SCP1 is equired for normal assembly of the central element of the synaptonemal complexes, as well as for normal centromere pairing during meiosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-229
Species: Mu, Rt, Ch, Ma, Pa, Pm, Hu(-)
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for SCP1 Antibody (NB300-229)(44)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Mammal, Monkey, Parasite.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
ICC/IF
(21)
IHC
(8)
IHC-P
(1)
IP
(1)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(31)
Rat
(1)
Mammal
(2)
Monkey
(1)
Parasite
(1)
All Species
Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using NB300-229 Applications Species
Lam KG, Brick K, Cheng G et al Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis. Nat Commun. 2019 Aug 23 [PMID: 31444359] (ICC/IF, Mouse)

Details:
Citation using the Biotin version of this antibody.
ICC/IF Mouse
Gruhn JR. Sex-specific differences in human meiotic recombination: A cytogenetic analysis of early meiotic events. Thesis. 2014 (ICC/IF, Mouse) ICC/IF Mouse
Spangenberg V, Arakelyan M, Cioffi MB et al. Cytogenetic mechanisms of unisexuality in rock lizards Sci Rep May 26 2020 [PMID: 32457493] (ICC/IF) ICC/IF
Hamada N, Hamazaki N, Shimamoto S et Al. Germ cell-intrinsic effects of sex chromosomes on early oocyte differentiation in mice PLoS Genet. Mar 26 2020 [PMID: 32214314] (IHC, Mouse) IHC Mouse
Zhang B, Tang Z, Li L, Lu LY NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis Cell Death Differ. Jan 21 2020 [PMID: 31965061] (ICC/IF, Mouse) ICC/IF Mouse
Zhang Y, Schroeder LK, Lessard MD et al. Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging Nat. Methods Jan 6 2020 [PMID: 31907447] (ICC/IF, Mouse, Human, Monkey) ICC/IF Mouse, Human, Monkey
Hua R, Wei H, Liu C et al. FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis Nucleic Acids Res. Nov 14 2019 [PMID: 31724724] (ICC/IF, Mouse) ICC/IF Mouse
Eyster C, Chuong HH, Lee CY, et al. The pericentromeric heterochromatin of homologous chromosomes remains associated after centromere pairing dissolves in mouse spermatocyte meiosis Chromosoma 2019 Jun 04 [PMID: 31165256] (ICC/IF, Mouse) ICC/IF Mouse
Tsang M, Gantchev J, Netchiporouk E et al. A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL) Oncotarget Dec 28 2018 [PMID: 30701021] (WB, Human) WB Human
Huang G, Kaufman AJ, Ryan RJH et al. Mouse DCUN1D1 (SCCRO) is required for spermatogenetic individualization. PLoS ONE Jan 17 2019 [PMID: 30653527] (ICC/IF, Mouse) ICC/IF Mouse
Show All 44 Publications.

Reviews for SCP1 Antibody (NB300-229) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB300-229:
Filter by Applications
ICC
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SCP1 NB300-229
Enlarge
5
reviewed by:
Anonymous
ICC Mouse 09/12/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse spermatozoa
SpeciesMouse
LotD2
Immunocytochemistry SCP1 NB300-229
Enlarge
5
reviewed by:
Daniela Moralli
ICC Mouse 04/11/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedSpermatocytes
SpeciesMouse
LotC-2

Comments

CommentsSpermatocytes cells fixed in PFA. Detected with anti-mouse 594

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for SCP1 Antibody (NB300-229) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-229AF350
Alexa Fluor 405 NB300-229AF405
Alexa Fluor 488 NB300-229AF488
Alexa Fluor 532 NB300-229AF532
Alexa Fluor 594 NB300-229AF594
Alexa Fluor 647 NB300-229AF647
Alexa Fluor 700 NB300-229AF700
Alexa Fluor 750 NB300-229AF750
Biotin NB300-229B
DyLight 350 NB300-229UV
DyLight 405 NB300-229V
DyLight 488 NB300-229G
DyLight 550 NB300-229R
DyLight 594 NB300-229DL594
DyLight 650 NB300-229C
DyLight 680 NB300-229FR
DyLight 755 NB300-229IR
FITC NB300-229F
HRP NB300-229H
Janelia Fluor 549 NB300-229JF549
Janelia Fluor 646 NB300-229JF646

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Array NB300-229

Bioinformatics Tool for SCP1 Antibody (NB300-229)

Discover related pathways, diseases and genes to SCP1 Antibody (NB300-229). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for SCP1 Antibody (NB300-229)

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Research Areas for SCP1 Antibody (NB300-229)

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Blogs on SCP1.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

SCP1 a Potential Cancer Target for Immunotherapy
Synaptonemal Complex Protein 1 (SCP1) is a novel tumor antigen that belongs to the growing family of cancer/testis antigens (CTA). SCP-1 is known to be selectively expressed during the meiotic prophase of spermatocytes and is involved in the pairing o...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
09/12/2019
Application: ICC
Species: Mouse

Daniela Moralli
04/11/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol SYCP1