SCP1 Antibody - BSA Free

Images

 
Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing ...read more
Western Blot: SCP1 Antibody [NB300-229] - Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 ...read more
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Double immunofluorescence of testicular spread preparations of the adult mice, SYCP3 (green) and SCP1 (red). Image collected and cropped by ...read more
Immunohistochemistry-Paraffin: SCP1 Antibody [NB300-229] - Punctate staining of murine SCP1 in mouse ovary using NB300-229.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - SCP1 labeled in mouse pachytene preparation (red), using NB300-229. CDK2 staining, near teleomeres, is also present (green).
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - Spermatocytes cells fixed in PFA. Detected with anti-mouse 594. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatozoa. Green: SCP1 staining. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatocyte. Red: scp1 colocalized with scp3 (green). ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale ...read more
Meiotic progression and chromosome paring in XX, XO and XY oocytes. (B) Pairing of homologous chromosomes in XX, XO and XY oocytes. Images show the immunofluorescence analysis of SYCP3 (white) and SYCP1 (red), and FISH ...read more
Meiotic spreads.Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) and SYCP1 (green), ...read more
hIAPP upregulates the HIF1 alpha /PFKFB3 stress pathway. a Summary of the differentially expressed genes of interest after microarray analysis25 performed on RNA isolated from wild type (WT) and hIAPP overexpressing ...read more
PRDX proteins bind to HIF-1 alpha and HIF-2 alpha A. HeLa cells were transfected with an expression vector encoding V5-epitope-tagged PRDX2 (PRDX2-V5) and exposed to 1% O2 for 24 h. Whole cell lysate (WCL) was subject ...read more
MRE11 associates with the DS region of OriP.A) DNA affinity purification assays were performed with Raji nuclear extracts and DNA templates derived from DS wt, DS nm-, or delta DS. Input and template bound proteins ...read more

Product Details

Summary
Reactivity Mu, Rt, Ch, Ma, Pa, Pm, Hu(-)Species Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

SCP1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to the C-terminus of the mouse SCP1 protein sequence. [UniProt# Q62209]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYCP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 27486799)
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:750
  • Immunohistochemistry 1:750
  • Immunohistochemistry-Frozen 1:750
  • Immunohistochemistry-Paraffin 1:750
  • Immunoprecipitation
  • Simple Western 1:100
  • Western Blot
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
SCP1 Peptide (NB300-229PEP)
Reviewed Applications
Read 3 Reviews rated 5
using
NB300-229 in the following applications:

Publications
Read Publications using
NB300-229 in the following applications:

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 25981592). Parasite reactivity reported in scientific literature (PMID: 27084479). Chicken reactivity reported in scientific literature (PMID: 28174243). Use in Monkey reported in scientific literature (PMID: 31907447). This antibody has not been shown to have human reactivity.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SCP1 Antibody - BSA Free

  • Cancer/testis antigen 8
  • CT8MGC104417
  • HOM-TES-14
  • SCP1SCP-1
  • synaptonemal complex protein 1

Background

Synaptonemal complex protein 1 is a major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. SCP1 is equired for normal assembly of the central element of the synaptonemal complexes, as well as for normal centromere pairing during meiosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-229
Species: Mu, Rt, Ch, Ma, Pa, Pm, Hu(-)
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP, ChIP

Publications for SCP1 Antibody (NB300-229)(54)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Mammal, Monkey, Parasite.

We have publications tested in 8 applications: Chemotaxis, ICC/IF, IF/IHC, IHC, IHC-P, IP, In Vivo, WB.


Filter By Application
Chemotaxis
(1)
ICC/IF
(25)
IF/IHC
(8)
IHC
(1)
IHC-P
(2)
IP
(1)
In Vivo
(1)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(37)
Rat
(1)
Mammal
(2)
Monkey
(1)
Parasite
(1)
All Species
Showing Publications 1 - 10 of 54. Show All 54 Publications.
Publications using NB300-229 Applications Species
Davies B, Zhang G, Moralli D et al. Characterization of meiotic recombination intermediates through gene knockouts in founder hybrid mice Genome research 2023-11-17 [PMID: 37977820] (IHC, Mouse)

Details:
Sample type: Testis
IHC Mouse
Faber EB, Sun L, Tang J et al. Development of allosteric and selective CDK2 inhibitors for contraception with negative cooperativity to cyclin binding Nature communications 2023-06-03 [PMID: 37270540] (ICC/IF, Mouse)

Details:
ICC/IF (on chromosome spreads): antibody incubation at 1:100)
ICC/IF Mouse
Hirano K, Nonami Y, Nakamura Y et al. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis Communications Biology 2022-05-26 [PMID: 35618762] (In Vivo) In Vivo
Zhang J, Zhou X, Wan D et al. TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice Frontiers in Cell and Developmental Biology 2022-04-25 [PMID: 35547804]
Lee S, Kuramochi-Miyagawa S, Nagamori I, Nakano T Effects of transgene insertion loci and copy number on Dnmt3L gene silencing through antisense transgene-derived PIWI-interacting RNAs RNA (New York, N.Y.) 2022-02-10 [PMID: 35145000] (ICC/IF, Mouse) ICC/IF Mouse
Kagiwada S, Aramaki S, Wu G et al. YAP creates epiblast responsiveness to inductive signals for germ cell fate Development (Cambridge, England) 2021-09-16 [PMID: 34528691] (ICC/IF, Mouse) ICC/IF Mouse
Takada Y, Yaman-Deveci R, Shirakawa T et al. Maintenance DNA methylation in pre-meiotic germ cells regulates meiotic prophase by facilitating homologous chromosome pairing Development (Cambridge, England) 2021-05-15 [PMID: 33998651]
Osawa Y, Murata K, Usui M et al. EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice eLife 2021-05-11 [PMID: 33973520] (IHC-P, Mouse) IHC-P Mouse
Larose H, Kent T, Ma Q et al. Regulation of meiotic progression by Sertoli-cell androgen signaling Molecular Biology of the Cell 2020-12-01 [PMID: 33026960] (ICC/IF) ICC/IF
Spangenberg V, Arakelyan M, Cioffi MB et al. Cytogenetic mechanisms of unisexuality in rock lizards Sci Rep 2020-05-26 [PMID: 32457493] (ICC/IF) ICC/IF
Show All 54 Publications.

Reviews for SCP1 Antibody (NB300-229) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB300-229:
Filter by Applications
ICC
(3)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SCP1 NB300-229
Enlarge
5
reviewed by:
Verified Customer
ICC Mouse 02/17/2021
View

Summary

ApplicationImmunocytochemistry
Sample Testedspermatocyte
SpeciesMouse
LotE3
Immunocytochemistry SCP1 NB300-229
Enlarge
5
reviewed by:
Verified Customer
ICC Mouse 09/12/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse spermatozoa
SpeciesMouse
LotD2
Immunocytochemistry SCP1 NB300-229
Enlarge
5
reviewed by:
Daniela Moralli
ICC Mouse 04/11/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedSpermatocytes
SpeciesMouse
LotC-2

Comments

CommentsSpermatocytes cells fixed in PFA. Detected with anti-mouse 594

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for SCP1 Antibody (NB300-229) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Array NB300-229

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Pathways for SCP1 Antibody (NB300-229)

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Research Areas for SCP1 Antibody (NB300-229)

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Blogs on SCP1.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

SCP1 a Potential Cancer Target for Immunotherapy
Synaptonemal Complex Protein 1 (SCP1) is a novel tumor antigen that belongs to the growing family of cancer/testis antigens (CTA). SCP-1 is known to be selectively expressed during the meiotic prophase of spermatocytes and is involved in the pairing o...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Verified Customer
02/17/2021
Application: ICC
Species: Mouse

Verified Customer
09/12/2019
Application: ICC
Species: Mouse

Daniela Moralli
04/11/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol SYCP1