Sodium Potassium ATPase Alpha 1 Antibody (464.6)

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Immunocytochemistry: Detection of ATPA1 (Green) in HepG2 cells using NB300-146. Nuclei (Blue) were counterstained using Hoechst 33258.

Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Staining of human enodmetrial glands within the uterus using NB300-146. Note the absence of staining in the surrounding myometrial smooth muscle.

Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified [NBP2-61137] - An intracellular stain was performed on A549 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified NBP2-61137 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image using the Purified form of this antibody.

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [Alexa Fluor® 647] [NBP2-61137AF647] - An intracellular stain was performed on A549 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NBP2-61137AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Dr, GP, Pm, Rb, Xp, Ye
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: 464.6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Sodium Potassium ATPase Alpha 1 Antibody (464.6) Summary

• Immunogen: Purified Sodium Potassium ATPase Alpha 1 from rabbit renal outer medulla. [UniProt# Q9N0Z6]
• Marker: Plasma Membrane Marker
• Specificity: This is specific for Na,K-ATPase alpha 1 subunit.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP1A1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
• Application Notes: This Sodium Potassium ATPase (464.6) antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and Western Blot. By Western Blot, a distinct band at 112 kDa is seen. Do not boil the sample prior to loading on the gel for Western Blot (60 degrees Celsius appears to work fine). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26941236). Publication with sheep shown however results may vary. Use in immunprecipitation reported in scientific literature (PMID 27748972).
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Sodium Potassium ATPase Alpha 1 Lysate (NBL1-07807)

Reactivity Notes

Human, bovine, porcine, monkey, murine, rabbit, guinea pig, canine, rat, Drosophila, Xenopus and Yeast.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Sodium Potassium ATPase Alpha 1 Antibody (464.6)

• ATPase, Na+/K+ transporting, alpha 1 polypeptide
• EC 3.6.3
• EC 3.6.3.9
• K-ATPase alpha-1 subunit
• K-ATPase catalytic subunit alpha-A protein
• MGC3285
• MGC51750
• Na
• Na(+)/K(+) ATPase alpha-1 subunit
• Na, K-ATPase, alpha-A catalytic polypeptide
• Na+, K+ ATPase alpha subunit
• Na+/K+ ATPase 1
• sodium pump 1
• Sodium pump subunit alpha-1
• sodium/potassium-transporting ATPase subunit alpha-1
• sodium-potassium-ATPase, alpha 1 polypeptide

Background

Sodium Potassium ATPase alpha 1 (ATP1A1) belongs to the P-type ATPase family and is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with exchange of sodium (Na+) and potassium (K+ ) ions across the cellular plasma membranes. This action creates an electrochemical gradient of Na+ and K+ ions, providing the energy for active transport of various nutrients. Its phosphorylation at Tyr-10 position alters the pumping activity whereas dephosphorylation mediated by PP2A (protein phosphatase 2A) following increases in intracellular Na+, leads to catalytic activity. Because the plasma membrane is highly permeable to water, it is the concentration of ions across this membrane that is critical for maintaining an adequate cell volume and sodium potassium ATPase is key player in this maintainance process because it provides the driving force for active Na+ as well as K+ transport into/out of the cell, with water following isosmotically. This pump is consists of equimolar ratios of two main subunits, the catalytic alpha subunit (which contains the binding sites for the mentioned cations, ATP, and cardiotonic steroid inhibitors) and the regulatory beta polypeptides (which is essential for the normal activity of the enzyme as well as involved potentially in the occlusion of K+ and modulation of the enzyme's K+ /Na+ affinity).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sodium Potassium ATPase Alpha 1 Antibody (NB300-146)(82)

Publications using NB300-146

• Jang C. A metabolite of branched chain amino acids drives vascular fatty acid transport and causes glucose intolerance. Thesis. 2016
• Saudrais E, Strazielle N, Ghersi-Egea JF. Choroid plexus glutathione peroxidases are instrumental in protecting the brain fluid environment from hydroperoxides during postnatal development. Am. J. Physiol., Cell Physiol. Jun 27 2018 [PMID: 29949405] (IHC, Rat)
• kong Y, Zhao L, Charette JR et al. A FRMD4B variant suppresses dysplastic photoreceptor lesions in models of enhanced S-cone syndrome and of Nrl deficiency. Hum. Mol. Genet. Jun 27 2018 [PMID: 29947801] (WB, Human)
• Yamashita K, Inagaki E, Hatou S et al. Corneal endothelial regeneration using mesenchymal stem cell derived from human umbilical cord Stem Cells Dev. Jun 21 2018 [PMID: 29929442] (Human)
• Kavanagh EL, Lindsay S, Halasz M et al. Protein and chemotherapy profiling of extracellular vesicles harvested from therapeutic induced senescent triple negative breast cancer cells. Oncogenesis. 2017 Oct 09 [PMID: 28991260] (WB, Human)
• Geng Z, Walsh PJ, Truong V et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS ONE. Mar 10 2017 [PMID: 28282420] (ICC/IF, Human)
• Uchida H, Machida M, Miura T et al. A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells JCI Insight Jan 12 2017 [PMID: 28097227] (ICC/IF, Human)
• Lobato-Alvarez JA, Roldan ML, Lopez-Murillo TD et al. The Apical Localization of Na(+), K(+)-ATPase in Cultured Human Retinal Pigment Epithelial Cells Depends on Expression of the beta2 Subunit. Front Physiol. Oct 24 2016 [PMID: 27774068] (WB, Human)
• Ontsouka CE, Huang X, Aliyev E, Albrecht C. In vitro characterization and endocrine regulation of cholesterol and phospholipid transport in the mammary gland. Mol Cell Endocrinol 2017 Jan 05 [PMID: 27760380]
• Sugio S, Tohyama K, Oku S et al. Astrocyte-mediated infantile-onset leukoencephalopathy mouse model. Glia Oct 17 2016 [PMID: 27748972] (WB, IP, Mouse)
• Paparelli L, Corthout N, Pavie B et al. Inhomogeneity Based Characterization of Distribution Patterns on the Plasma Membrane. PLoS Comput. Biol. Sep 1 2016 [PMID: 27603951] (WB)
• Jiang Y, Woosley AN, Sivalingam N et al. Cathepsin-B-mediated cleavage of Disabled-2 regulates TGF- Beta -induced autophagy Nat. Cell Biol. 2016 Aug 01 [PMID: 27398911]
• Jang C, Oh SF, Wada S et al. A branched-chain amino acid metabolite drives vascular fatty acid transport and causes insulin resistance. Nat. Med. 2016 Mar 07 [PMID: 26950361]
• Strazielle N, Creidy R, Malcus C et al. T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium. PLoS ONE. 2016 Mar 05 [PMID: 26942913] (ICC/IF, Rat)
• Liu W, Edin F, Blom H et al. Super-resolution structured illumination fluorescence microscopy of the lateral wall of the cochlea: the Connexin26/30 proteins are separately expressed in man. Cell Tissue Res. 2016 Mar 04 [PMID: 26941236] (IHC-Fr, Human)
• Luo K, Thaete LG, Neerhof MG. Endothelin Receptor A Antagonism and Fetal Growth in Endothelial Nitric Oxide Synthase Gene Knockout Maternal and Fetal Mice. Reprod Sci 2016 Aug [PMID: 26791973]
• Tokuda S, Kim YH, Matsumoto H et al. Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia. PLoS One 2015 [PMID: 26716691]
• Yan Q, Tang S, Tan Z et al. Proteomic Analysis of Isolated Plasma Membrane Fractions from the Mammary Gland in Lactating Cows. J. Agric. Food Chem. 2015 Aug 26 [PMID: 26237224] (WB, Bovine)
• Huang Bin, Wang Dao-Xin, Deng Wang. Protective effects of dexamethasone on early acute lung injury induced by oleic acid in rats. Int J Clin Exp Med. 2014 Dec 15 [PMID: 25663967] (Rat)
• Sutt S, Altpere A, Reimets R et al. Wfs1-deficient animals have brain-region-specific changes of na(+), K(+) -ATPase activity and mRnA expression of Alpha1 and B1 subunits. J. neurosci. Res. 2014 Nov 10 [PMID: 25385034] (WB, Mouse)
• Lungkaphin A, Arjinajarn P, Pongchaidecha A et al. Impaired insulin signaling affects renal organic anion transporter 3 (oat3) function in streptozotocin-induced diabetic rats. PLoS ONE. 2014 May 07 [PMID: 24801871] (WB, Rat)
• Ohtake N, Saito M, Eto M et al. Exendin-4 promotes the membrane trafficking of the AMPA receptor GluR1 subunit and ADAM10 in the mouse neocortex. Regul. Pept. 2014 Apr 24 [PMID: 24769307] (WB, Mouse)
• Fan N, Sun H, Wang Y et al. Midkine, a Potential Link between Obesity and Insulin Resistance. PLoS ONE 2014 Feb 11 [PMID: 24516630] (WB, Mouse)
• Wang Q, Schultz BD. Cholera toxin enhances Na(+) absorption across MCF10A human mammary epithelia. Am. J. Physiol., Cell Physiol. 2014 Mar 01 [PMID: 24371040] (WB, Human)
• Drivas TG, Holzbaur EL, Bennett J. Disruption of CEP290 microtubule/membrane-binding domains causes retinal degeneration. J Clin Invest. 2013 Oct 1 [PMID: 24051377] (WB, Human)
• Aggelis V, Craven RA, Peng J et al. VHL-dependent regulation of a beta-dystroglycan glycoform and glycogene expression in renal cancer. Int J Oncol. 2013 Nov [PMID: 23970118] (WB, Human)
• Ontawong A, Saowakon N, Vivithanaporn P et al. Antioxidant and Renoprotective Effects of Spirogyra neglecta (Hassall) Kutzing Extract in Experimental Type 2 Diabetic Rats. Biomed Res Int 2013 [PMID: 23862157] (WB, Rat)
• Jang HS, Han SJ, Kim JI et al. Activation of ERK accelerates repair of renal tubular epithelial cells, whereas it inhibits progression of fibrosis following ischemia/reperfusion injury. Biochim Biophys Acta 2013 Dec [PMID: 23851027] (IHC, ICC/IF, Mouse)
• Schauer KL, Freund DM, Prenni JE, Curthoys NP. Proteomic Profiling and Pathway Analysis of the Response of Rat Renal Proximal Convoluted Tubules to Metabolic Acidosis. Am J Physiol Renal Physiol 2013 Jun 26 [PMID: 23804448] (WB, Rat)
• Raksaseri P, Chatsudthipong V, Muanprasat C, Soodvilai S. Activation of liver X receptors reduces cystic fibrosis transmembrane conductance regulator (CFTR)-mediated chloride transport in kidney collecting duct cells. Am J Physiol Renal Physiol 2013 May 29 [PMID: 23720350] (WB, Canine)
• Feduska JM, Garcia PL, Brennan SB et al. N-glycosylation of ICAM-2 is required for ICAM-2-mediated complete suppression of metastatic potential of SK-N-AS neuroblastoma cells. BMC Cancer 2013 May 28 [PMID: 23714211] (WB, Human)
• Hatou S, Higa K, Inagaki E et al. Validation of Na,K-ATPase Pump Function of Corneal Endothelial Cells for Corneal Regenerative Medicine. Tissue Eng Part C Methods 2013 May 14 [PMID: 23544359] (ICC/IF, Human, Rabbit)
• Matta S, Van Kolen K, da Cunha R et al. LRRK2 Controls an EndoA Phosphorylation Cycle in Synaptic Endocytosis Neuron 2012 Sep 20 [PMID: 22998870] (WB, Drosophila)
• Yoon HE, Lim SW, Piao SG. Statin Upregulates the Expression of Klotho, an Anti-Aging Gene, in Experimental Cyclosporine Nephropathy Nephron Exp Nephrol 2012 Sep 13 [PMID: 22986347]
• Le Goff A, Ji Z, Leclercq B et al. Anti-apoptotic role of caspase-cleaved GAB1 in HGF/SF-MET signaling J Biol Chem 2012 Aug 22 [PMID: 22915589] (WB, Canine)
• Shao H, Lee S, Gae-Scott S et al. Extracellular Matrix Lumican Promotes Bacterial Phagocytosis, and Lum/Mice Show Increased Pseudomonas aeruginosa Lung Infection Severity J Biol Chem 2012 Oct 19 [PMID: 22865855] (ICC/IF, Mouse)
• Desmoulin SK, Wang L, Polin L et al. Functional Loss of the Reduced Folate Carrier Enhances the Antitumor Activities of Novel Antifolates with Selective Uptake by the Proton-coupled Folate Transporter Mol Pharmacol 2012 Jun 26 [PMID: 22740639]
• Chun KH, Araki K, Jee Y, Lee DH, Oh BC, Huang H, Park KS, Lee SW, Zabolotny JM, Kim YB. Regulation of glucose transport by ROCK1 differs from that of ROCK2 and is controlled by actin polymerization. Endocrinology;153(4):1649-62. 2012 Apr. [PMID: 22355071] (WB, Mouse)
• Larsson S, Resjo S, Gomez MF, James P, Holm C. Characterization of the lipid droplet proteome of a clonal insulin-producing ?-cell line (INS-1 832/13). J Proteome Res;11(2):1264-73. 2012 Feb 3. [PMID: 22268682] (WB, Rat)
• Garg AD, Krysko DV, Verfaillie T et al. A novel pathway combining calreticulin exposure and ATP secretion in immunogenic cancer cell death. The EMBO Journal. 2012 Jan 17. [PMID: 22252128]
• Thimiri Govinda Raj DB, Ghesquiere B, Tharkeshwar AK et al. A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes. Mol Syst Biol;7:541. 2011 [PMID: 22027552]
• Nonomura K, Arai Y, Mitani H et al. Insulin down-regulates specific activity of ATP-binding cassette transporter A1 for high density lipoprotein biogenesis through its specific phosphorylation. Atherosclerosis. 2011 Feb 22. [PMID: 21402379]
• Mushtaq M, Nam T-S, Kim U-H. Critical role for CD38-mediated Ca2+ signaling in thrombin-induced procoagulant activity of mouse platelets and hemostasis. J Biol Chem. 2011 Feb 21. [PMID: 21339289]
• Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J. Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? Proteomics Clin Appl. Proteomics Clin Appl. 1(11):1446-61. doi: 10.1002/prca.200700522. 2007 Nov. [PMID: 21136642]
• Lewandowski NM, Ju S, Verbitsky M et al. Polyamine pathway contributes to the pathogenesis of Parkinson disease. Proc Natl Acad Sci U S A 2010 Sep 28 [PMID: 20837543]
• Li H, Ganta S, Fong P. Altered ion transport by thyroid epithelia from CFTR(-/-) pigs suggests mechanisms for hypothyroidism in cystic fibrosis. Exp Physiol;95(12):1132-44. 2010 Dec. [PMID: 20729267] (WB, Porcine)
• Tanaka N, Abe-Dohmae S, Iwamoto N et al. Helical apolipoproteins of high-density lipoprotein enhance phagocytosis by stabilizing ATP-binding cassette transporter A7. J Lipid Res 2010 Sep [PMID: 20495215]
• Zhang Y, Liu R, Ni M, Gill P, Lee AS. Cell surface relocalization of the endoplasmic reticulum chaperone and unfolded protein response regulator GRP78/BiP. J Biol Chem;285(20):15065-75. 2010 May 14. [PMID: 20208072]
• Cottrell GS, Padilla BE, Amadesi S et al. Endosomal Endothelin-converting Enzyme-1: A REGULATOR OF {beta}-ARRESTIN-DEPENDENT ERK SIGNALING. J Biol Chem;284(33):22411-22425. 2009 [PMID: 19531493] (WB, Human, Mouse, Rat)
• Feldmann A, Winterstein C, White R, Trotter J, Kramer-Albers EM. Comprehensive analysis of expression, subcellular localization, and cognate pairing of SNARE proteins in oligodendrocytes. J Neurosci Res;87(8):1760-72. 2009 Jun. [PMID: 19185015]
• Nirmalan NJ, Harnden P, Selby PJ, Banks RE. Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting. J Pathol;217(4):497-506. 2009 Mar. [PMID: 19156775]
• Peyrottes I, Navarro V, Ondo-Mendez A et al. Immunoanalysis indicates that the sodium iodide symporter is not overexpressed in intracellular compartments in thyroid breast cancers. Eur J Endocrinol;160(2):215-25. 2009 Feb. [PMID: 19029227]
• Johansson P, Dziegielewska K, Saunders N. Low levels of Na, K-ATPase and carbonic anhydrase II during choroid plexus development suggest limited involvement in early CSF secretion. Neurosci Lett 2008 Sep 5 [PMID: 18602969] (Rat)
• Sironi C, Bodega F, Porta C et al. Na+-glucose cotransporter is also expressed in mesothelium of species with thick visceral pleura. Respir Physiol Neurobiol 2008 May 31 [PMID: 18424241] (WB, Sheep)
• Dayem M, Basquin C, Navarro V, Carrier P, Marsault R, Chang P, Huc S, Darrouzet E, Lindenthal S, Pourcher T. Comparison of expressed human and mouse sodium/iodide symporters reveals differences in transport properties and subcellular localization. J Endocrinol;197(1):95-109. 2008 Apr. [PMID: 18372236]
• Burgess, B, Parkinson, P, Racke, M et al. ABCG1 influences the brain cholesterol biosynthetic pathway but does not affect amyloid precursor protein or apolipoprotein E metabolism in vivo. J Lipid Res;49(6):1254-67. 2008 Jun. [PMID: 18314463]
• Winterstein C, Trotter J, Kramer-Albers EM. Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling. J Cell Sci;121(Pt 6):834-42. 2008 Mar 15. [PMID: 18303048]
• Kadereit,B et al. Evolutionarily conserved gene family important for fat storage. Proc Natl Acad Sci U S A;105(1):94-9. 2008 Jan 8. [PMID: 18160536]
• Padilla, BE et al. Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins. J Cell Biol;179(5):981-97. 2007 Dec 3. [PMID: 18039931]
• Sironi C, Bodega F, Porta C et al. Expression of Na+-glucose cotransporter (SGLT1) in visceral and parietal mesothelium of rabbit pleura. Respir Physiol Neurobiol 2007 Oct 15 [PMID: 17652034] (WB, Rabbit)
• Jahn, T et al. Lipid rafts are required for Kit survival and proliferation signals. Blood;110(6):1739-47. 2007 Sep 15. [PMID: 17554062]
• Qu, Shen et al. Effects of apoA-V on HDL and VLDL metabolism in APOC3 transgenic mice. J Lipid Res;48(7):1476-87. 2007 Jul. [PMID: 17438339]
• Roach W, Plomann M. PACSIN3 overexpression increases adipocyte glucose transport through GLUT1. Biochem Biophys Res Commun 2007 Apr 13 [PMID: 17320047]
• Tansley, G H et al. The cholesterol transporter ABCG1 modulates the subcellular distribution and proteolytic processing of beta-amyloid precursor protein. J Lipid Res;48(5):1022-34. 2007 May. [PMID: 17293612]
• Perry JL, Dembla-Rajpal N, Hall LA, Pritchard JB. A three-dimensional model of human organic anion transporter 1: aromatic amino acids required for substrate transport. J Biol Chem;281(49):38071-9. 2006 Dec 8. [PMID: 17038320] (WB, Xenopus)
• Pessach I, Shmelzer Z, Leto TL, Dinauer MC, Levy R. The C-terminal flavin domain of gp91phox bound to plasma membranes of granulocyte-like X-CGD PLB-985 cells is sufficient to anchor cytosolic oxidase components and support NADPH oxidase-associated diaphorase activity independent of cytosolic phospholipase A2 regulation. J Leukoc Biol;80(3):630-9. 2006 Sep. [PMID: 16844764]
• Tarn, C et al. Therapeutic effect of imatinib in gastrointestinal stromal tumors: AKT signaling dependent and independent mechanisms. Cancer Res;66(10):5477-86. 2006 May 15. [PMID: 16707477]
• Chen P, Guzman JP, Leong PK, Yang LE, Perianayagam A, Babilonia E, Ho JS, Youn JH, Wang WH, McDonough AA. Modest dietary K+ restriction provokes insulin resistance of cellular K+ uptake and phosphorylation of renal outer medulla K+ channel without fall in plasma K+ concentration. Am J Physiol Cell Physiol;290(5):C1355-63. 2006 May. [PMID: 16354756]
• Leong PK, Devillez A, Sandberg MB, Yang LE, Yip DK, Klein JB, McDonough AA. Effects of ACE inhibition on proximal tubule sodium transport. Am J Physiol Renal Physiol;290(4):F854-63. 2006 Apr. [PMID: 16263808]
• Cross, WR et al. A biomimetic tissue from cultured normal human urothelial cells: analysis of physiological function. Am J Physiol Renal Physiol;289(2):F459-68. 2005 Aug. [PMID: 15784840]
• Shah, M et al. Monocrotaline pyrrole-induced endothelial cell megalocytosis involves a Golgi blockade mechanism. Am J Physiol Cell Physiol;288(4):C850-62. 2005 Apr. [PMID: 15561761] (WB, Bovine)
• Pohl, J et al. FAT/CD36-mediated long-chain fatty acid uptake in adipocytes requires plasma membrane rafts. Mol Biol Cell;16(1):24-31. 2005 Jan. [PMID: 15496455]
• Holzer, A et al. Cisplatin rapidly down-regulates its own influx transporter hCTR1 in cultured human ovarian carcinoma cells. Clin Cancer Res;10(19):6744-9. 2004 Oct 1. [PMID: 15475465]
• Knox, W et al. Novel role of phospholipase C-delta1: regulation of liver mitochondrial Ca2+ uptake. Am J Physiol Gastrointest Liver Physiol;287(3):G533-40. 2004 Sep. [PMID: 15107298]
• Xavier, S et al. Amelioration of Radiation-induced Fibrosis: Inhibition of Transformation Growth Factor-beta Signaling by Halofuginone. J Biol Chem;279(15):15167-76. 2004 Apr 9. [PMID: 14732719]
• Yang LE, Zhong H, Leong PK, Perianayagam A, Campese VM, McDonough AA. Chronic renal injury-induced hypertension alters renal NHE3 distribution and abundance. Am J Physiol Renal Physiol;284(5):F1056-65. 2003 May. [PMID: 12554635]
• Wang J, Velotta JB, McDonough AA, Farley RA. All human Na(+)-K(+)-ATPase alpha-subunit isoforms have a similar affinity for cardiac glycosides. Am J Physiol Cell Physiol;281(4):C1336-43. 2001 Oct. [PMID: 11546672] (WB, Human)
• Farley RA, Elquza E, Muller-Ehmsen J, Kane DJ, Nagy AK, Kasho VN, Faller LD. 18O-exchange evidence that mutations of arginine in a signature sequence for P-type pumps affect inorganic phosphate binding. Biochemistry;40(21):6361-70. 2001 May 29. [PMID: 11371198] (WB, Yeast)
• Magyar CE, Zhang Y, Holstein-Rathlou NH, McDonough AA. Proximal tubule Na transporter responses are the same during acute and chronic hypertension. Am J Physiol Renal Physiol;279(2):F358-69. 2000 Aug. [PMID: 10919857]
• Zhang Y, Norian JM, Magyar CE, Holstein-Rathlou NH, Mircheff AK, McDonough AA. In vivo PTH provokes apical NHE3 and NaPi2 redistribution and Na-K-ATPase inhibition. Am J Physiol;276(5 Pt 2):F711-9. 1999 May. [PMID: 10330053]
• Zhang Y, Magyar CE, Norian JM, Holstein-Rathlou NH, Mircheff AK, McDonough AA. Reversible effects of acute hypertension on proximal tubule sodium transporters. Am J Physiol;274(4 Pt 1):C1090-100. 1998 Apr. [PMID: 9575807]
• McDonough AA, Zhang Y, Shin V, Frank JS. Subcellular distribution of sodium pump isoform subunits in mammalian cardiac myocytes. Am J Physiol;270(4 Pt 1):C1221-7. 1996 Apr. [PMID: 8928749] (IHC, Rat, Guinea Pig)

Reviews for Sodium Potassium ATPase Alpha 1 Antibody (NB300-146) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Haiquan Lu WB Human 08/22/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell
• Species: Human

Blocking

• Blocking Details: Blocking with 4% BSA for 30 min

Primary Anitbody

• Dilution Ratio: 1:1000, overnight

Details

• Detection Notes: Regular Protocol

reviewed by: Dan Shan WB Human 08/21/2012

Summary

• Application: Western Blot
• Sample Tested: Human Postmortem prefrontal cortex tissue
• Species: Human
• Comments: used 4-12% gradient gel

Blocking

• Blocking Details: The membrane was blocked with LiCor blocking buffer for 1h at room temperature

Primary Anitbody

• Dilution Ratio: 1:500, overnight at 4C with LiCor blocking buffer

Secondary Antibody

• Secondary Description: Donkey anti-mouse IgG
• Secondary Concentration: 1:5000

Comments

• Comments: used 4-12% gradient gel

reviewed by: Alexandra Fragniere WB Human 12/02/2010

Summary

• Application: Western Blot
• Sample Tested: U87MG whole cell lysate
• Species: Human
• Lot: A23
• Comments: Whole cell extract from U87MG cell line. Ladder [kDa]: 260, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10. Used 10% SDS gel.

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:5000

Secondary Antibody

• Secondary Description: Secondary Ab Dilution Ratio: 1:10,000

Comments

• Comments: Whole cell extract from U87MG cell line. Ladder [kDa]: 260, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10. Used 10% SDS gel.

FAQs for Sodium Potassium ATPase Alpha 1 Antibody (NB300-146). (Showing 1 - 3 of 3 FAQs).

1. I am looking forward to pick up a plasma membrane marker. I have gastric cancer cell line and I am preparing PM out of that. To just check the quality of prep, I need a plasma membrane marker.
   • Our sodium potassium ATPase and plasma membrane products can be found using this links. I hope this helps. Please contact us at technical@novusbio.com with any additional questions.
2. I would like to know whether it is possible to dilute this antibody in 50% glycerol final, and keep the aliquots at -20 degrees Celsius?
   • As far as the storage conditions are concerned, since this antibody is supplied as ascites and may contain proteases, it has to be frozen immediately at -20 or -80. From a technical perspective, adding glycerol as a cryoprotectant to avoid freeze/thaw damage should be acceptable; however, we suggest to store our antibodies as recommended in the datasheet which in this case it is better to aliquot the antibody in small volumes and store it at -20 or -80. We do supply some antibodies that we recommend adding glycerol but NB300-146 has not been tested in this storage condition.
3. Was the epitope characterized?
   • We unfortunately have not mapped the epitope or binding region for this product yet. Our lab will always include 0.1% Tween-20 in their diluent and wash buffers for ICC staining, so I would recommend using this detergent for your customers staining. If they want to try first without the detergent, that is fine as well.

Bioinformatics

• Gene Symbol: ATP1A1
• Entrez:
  • 476(Human)
  • 25650(Rat)
• Uniprot:
  • P05023(Human)