Sodium Potassium ATPase Alpha 1 Antibody (464.6)

Images

 
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Transfected SK-N-AS cells express ICAM-2 transcripts and proteins. ICAM-2 WT and variants localized to cell membranes. Experimental details ...read more
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier. Immunofluorescent staining of Na+K+ ATPase and ABCC1, showing the ...read more
Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Staining of human enodmetrial glands within the uterus using NB300-146. Note the absence of staining in the surrounding myometrial ...read more
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on NIH3T3 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched ...read more
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% ...read more
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% ...read more
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Detection of ATPA1 (Green) in HepG2 cells using NB300-146. Nuclei (Blue) were counterstained using Hoechst 33258.
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier. Immunofluorescent staining of Na+K+ ATPase and ABCC1, showing the ...read more
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - ARPE-19 cells cultured on transwell inserts for 4 weeks are not completely polarized. ARPE-19 cells were cultured ...read more
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with NB300-146 and a matched isotype control. Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with NBP2-61137AF647 (Blue) and a matched isotype control (Orange). Cells were fixed ...read more
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with - Purified NBP2-61137 and a matched isotype control. Cells were fixed with 4% PFA ...read more
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on RCC4 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched ...read more
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on SK-MEL-28 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched ...read more
Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - ARPE-19 cells cultured on transwell inserts for 4 weeks are not completely polarized. ARPE-19 cells were cultured up to 4 weeks on transwell inserts covered ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Dr, Gp, Pm, Rb, Sh, Xp, YeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
464.6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Sodium Potassium ATPase Alpha 1 Antibody (464.6) Summary

Immunogen
Purified Sodium Potassium ATPase Alpha 1 from rabbit renal outer medulla. [UniProt# Q9N0Z6]
Marker
Plasma Membrane Marker
Specificity
This is specific for Na,K-ATPase alpha 1 subunit.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ATP1A1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:10000
Application Notes
In Western Blot, a distinct band at ~ 112 kDa is seen. Do not boil the sample prior to loading on the gel for Western Blot (60 degrees Celsius appears to work fine). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26941236). Publication with sheep shown (PMID: 18424241); however results may vary. Use in immunoprecipitation reported in scientific literature (PMID: 27748972).
Theoretical MW
112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Sodium Potassium ATPase Alpha 1 Overexpression Lysate
Reviewed Applications
Read 4 Reviews rated 4.3
using
NB300-146 in the following applications:

Publications
Read Publications using
NB300-146 in the following applications:

  • 10 publications
  • IHC
    6 publications
  • 1 publication
  • IP
    1 publication
  • WB
    37 publications

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 18424241).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Sodium Potassium ATPase Alpha 1 Antibody (464.6)

  • ATP1A1
  • ATPase, Na+/K+ transporting, alpha 1 polypeptide
  • EC 3.6.3
  • EC 3.6.3.9
  • EC 7.2.2.13
  • K-ATPase alpha-1 subunit
  • K-ATPase catalytic subunit alpha-A protein
  • MGC3285
  • MGC51750
  • Na
  • Na(+)/K(+) ATPase alpha-1 subunit
  • Na, K-ATPase, alpha-A catalytic polypeptide
  • Na+, K+ ATPase alpha subunit
  • Na+/K+ ATPase 1
  • Sodium Potassium ATPase Alpha 1
  • sodium pump 1
  • Sodium pump subunit alpha-1
  • sodium/potassium-transporting ATPase subunit alpha-1
  • sodium-potassium-ATPase, alpha 1 polypeptide

Background

Sodium Potassium ATPase alpha 1 (ATP1A1) belongs to the P-type ATPase family and is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with exchange of sodium (Na+) and potassium (K+ ) ions across the cellular plasma membranes. This action creates an electrochemical gradient of Na+ and K+ ions, providing the energy for active transport of various nutrients. Its phosphorylation at Tyr-10 position alters the pumping activity whereas dephosphorylation mediated by PP2A (protein phosphatase 2A) following increases in intracellular Na+, leads to catalytic activity. Because the plasma membrane is highly permeable to water, it is the concentration of ions across this membrane that is critical for maintaining an adequate cell volume and sodium potassium ATPase is key player in this maintainance process because it provides the driving force for active Na+ as well as K+ transport into/out of the cell, with water following isosmotically. This pump is consists of equimolar ratios of two main subunits, the catalytic alpha subunit (which contains the binding sites for the mentioned cations, ATP, and cardiotonic steroid inhibitors) and the regulatory beta polypeptides (which is essential for the normal activity of the enzyme as well as involved potentially in the occlusion of K+ and modulation of the enzyme's K+ /Na+ affinity).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for Sodium Potassium ATPase Alpha 1 Antibody (NB300-146)(102)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Bovine, Canine, Drosophila, Guinea Pig, Porcine, Rabbit, Sheep, Xenopus, Yeast.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IP, WB.


Filter By Application
ICC/IF
(10)
IHC
(6)
IHC-Fr
(1)
IP
(1)
WB
(37)
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Filter By Species
Human
(16)
Mouse
(17)
Rat
(11)
Bovine
(2)
Canine
(2)
Drosophila
(1)
Guinea Pig
(2)
Porcine
(1)
Rabbit
(3)
Sheep
(1)
Xenopus
(1)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 102. Show All 102 Publications.
Publications using NB300-146 Applications Species
Wang Z, Yang X, Wang X et al. Glycyrrhizin attenuates caspase-11-dependent immune responses and coagulopathy by targeting high mobility group box 1 International immunopharmacology Mar 24 2022 [PMID: 35339905] (WB, Mouse, Human) WB Mouse, Human
Wong BH, Mei D, Chua GL et al. The lipid transporter Mfsd2a maintains pulmonary surfactant homeostasis The Journal of biological chemistry Feb 9 2022 [PMID: 35150739] (Mouse) Mouse
Miwa T, Wei FY, Tomizawa K Cdk5 regulatory subunit-associated protein 1 knockout mice show hearing loss phenotypically similar to age-related hearing loss Molecular brain May 17 2021 [PMID: 34001214] (IHC, Mouse) IHC Mouse
Kravtsova VV, Paramonova II, Vilchinskaya NA et al. Chronic Ouabain Prevents Na,K-ATPase Dysfunction and Targets AMPK and IL-6 in Disused Rat Soleus Muscle Int J Mol Sci Apr 30 2021 [PMID: 33920198] (WB, Rat)

Details:
Citation using the HRP format of this antibody.
WB Rat
Miwa T Protective Effects of N1-Methylnicotinamide Against High-Fat Diet- and Age-Induced Hearing Loss via Moderate Overexpression of Sirtuin 1 Protein Frontiers in cellular neuroscience Apr 6 2021 [PMID: 33889076] (IHC, Mouse) IHC Mouse
Wang X, Li Z, Bai Y et al. A small molecule binding HMGB1 inhibits caspase-11-mediated lethality in sepsis Cell death & disease Apr 14 2021 [PMID: 33854044]
O'Connor C, Wallace-Povirk A, Ning C et al. Folate transporter dynamics and therapy with classic and tumor-targeted antifolates Scientific reports Mar 18 2021 [PMID: 33737637] (WB) WB
Tang Y, Wang X, Li Z et al. Heparin prevents caspase-11-dependent septic lethality independent of anticoagulant properties Immunity Feb 8 2021 [PMID: 33561388]
Yoon S, Piguel NH, Khalatyan N, et al. Homer1 promotes dendritic spine growth through ankyrin-G and its loss reshapes the synaptic proteome Molecular psychiatry Jan 4 2021 [PMID: 33398084] (WB, Mouse) WB Mouse
van Beelen ESA, van der Valk WH, de Groot JCMJ et al. Migration and fate of vestibular melanocytes during the development of the human inner ear Dev Neurobiol Oct 19 2020 [PMID: 33075185] (IHC, Human) IHC Human
Show All 102 Publications.

Reviews for Sodium Potassium ATPase Alpha 1 Antibody (NB300-146) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using NB300-146:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(4)
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Images Ratings Applications Species Date Details
Western Blot Sodium Potassium ATPase Alpha 1 NB300-146
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4
reviewed by:
Teddy Chan
WB Human 02/08/2019
View

Summary

ApplicationWestern Blot
Sample TestedHuman microglia cell lysate
SpeciesHuman
LotB-5

Comments

CommentsNOT boiling the samples gives a much better signal.
  3
reviewed by:
Verified Customer
WB Human 08/22/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman
Western Blot Sodium Potassium ATPase Alpha 1 NB300-146
Enlarge
5
reviewed by:
Verified Customer
WB Human 08/21/2012
View

Summary

ApplicationWestern Blot
Sample TestedHuman Postmortem prefrontal cortex tissue
SpeciesHuman
Western Blot Sodium Potassium ATPase Alpha 1 NB300-146
Enlarge
5
reviewed by:
Verified Customer
WB Human 12/02/2010
View

Summary

ApplicationWestern Blot
Sample TestedU87MG whole cell lysate
SpeciesHuman
LotA23

Product General Protocols

View specific protocols for Sodium Potassium ATPase Alpha 1 Antibody (NB300-146): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sodium Potassium ATPase Alpha 1 Antibody (NB300-146). (Showing 1 - 3 of 3 FAQs).

  1. I am looking forward to pick up a plasma membrane marker. I have gastric cancer cell line and I am preparing PM out of that. To just check the quality of prep, I need a plasma membrane marker.
    • Our sodium potassium ATPase and plasma membrane products can be found using this links. I hope this helps. Please contact us at technical@novusbio.com with any additional questions.
  2. I would like to know whether it is possible to dilute this antibody in 50% glycerol final, and keep the aliquots at -20 degrees Celsius?
    • As far as the storage conditions are concerned, since this antibody is supplied as ascites and may contain proteases, it has to be frozen immediately at -20 or -80. From a technical perspective, adding glycerol as a cryoprotectant to avoid freeze/thaw damage should be acceptable; however, we suggest to store our antibodies as recommended in the datasheet which in this case it is better to aliquot the antibody in small volumes and store it at -20 or -80. We do supply some antibodies that we recommend adding glycerol but NB300-146 has not been tested in this storage condition.
  3. Was the epitope characterized?
    • We unfortunately have not mapped the epitope or binding region for this product yet. Our lab will always include 0.1% Tween-20 in their diluent and wash buffers for ICC staining, so I would recommend using this detergent for your customers staining. If they want to try first without the detergent, that is fine as well.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.3
5
2
4
1
3
1
2
0
1
0

Teddy Chan
02/08/2019
Application: WB
Species: Human

 
Verified Customer
08/22/2015
Application: WB
Species: Human

Verified Customer
08/21/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATP1A1
Entrez
Uniprot