SCP3/SYCP3 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Chromosome spread for sycp3. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC analysis of a formalin fixed and paraffin embedded tissue section of Mouse testis using SCP3 antibody at 1:1000 dilution. The signal was detected ...read more
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Exposure to DEHP impairs meiotic progression of oocytes from pachytene to diplotene. Immunolabeling of the oocyte chromosomes with anti-SYCP3 ...read more
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Loss of lamin C2 has no effect on meiotic telomere attachment. Chromosome spread preparations of pachytene-like lamin C2-/- spermatocytes ...read more
Western Blot: SCP3/SYCP3 Antibody [NB300-232] - SCP3 Antibody [NB300-232] - Analysis of SCP3 in mouse testis protein.
Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - Analysis of SCP3 in human testis using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - SCP3 Antibody [NB300-232] - SCP3 labeled in mouse pachytene preparation (red), using NB300-232 SCP3 antibody. CDK2 staining, near teleomeres, is ...read more
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Loss of lamin C2 has no effect on meiotic telomere attachment.. Chromosome spread preparations of pachytene-like lamin C2-/- spermatocytes ...read more
Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC-P analysis of formalin fixed paraffin embedded tissue section of mouse testes using SCP3 antibody at 1:200 dilution. Specific staining may be seen in ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Fe, PaSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

SCP3/SYCP3 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminal region of the human SCP3 protein. [UniProt# Q8IZU3]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYCP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen 1:200-1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation
Application Notes
This SCP3 antibody is useful for Immunofluorescence, Immunohistochemistry (Frozen and Paraffin), Immunocytochemistry and Western blot where a band is seen at ~28 kDa. Use in immunoblotting reported in scientific literature (PMID: 27612028). Use in immunoprecipitation reported in scientific literature (PMID: 18694876).
Control
SCP3/SYCP3 Overexpression Lysate
Control Peptide
SCP3/SYCP3 Peptide (NB300-232PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-232 in the following applications:

Publications
Read Publications using
NB300-232 in the following applications:

  • IB
    1 publication
  • 21 publications
  • IHC
    10 publications
  • 1 publication
  • 3 publications
  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Rat, pig, bovine and mouse. Chicken reactivity reported in scientific literature (PMID: 26096940). Parasite, and Feline reactivity reported in scientific literature (PMID: 27084479). Human reactivity has been observed in IHC. Human reactivity reported in scientific literature (PMID: 29231814).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SCP3/SYCP3 Antibody

  • anti-SCP3
  • COR1
  • MGC71888
  • SCP3 chromosome 3 open reading frame 8
  • SCP3 COR1
  • SCP3
  • SCP-3
  • SYCP3
  • synaptonemal complex protein 3

Background

SCP3 (Synaptonemal complex protein 3) is a DNA-binding nuclear protein that belongs to the XLR/SYCP3 family and is an essential structural component of the transverse filaments of synaptonemal complexes formed between homologous chromosomes during meiotic prophase. The synaptonemal complex develops during prophase of first meiosis, wherein it allows the synapse as well as recombination between maternal - paternal chromosomes, and SCP3 is essential to hold cohesin complex between homologous chromosomes, which avoid premature segregation thereby preventing aneuploidies. SCP3 is involved in phosphorylation of HORMAD1 and HORMAD2. It is essential to development of testis (where it is primarily localized) and defective SCP3 may leads to spermatogenic failure type 4 (SPGF4). SCP3 deificient mouse depicts high numbers of aneuploidies in oocytes and offspring. SCP3 is also expressed in certain malignancies including human acute lymphatic leukemias, cervical cancer cells, lung cancer cells etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SCP3/SYCP3 Antibody (NB300-232)(54)

We have publications tested in 7 confirmed species: Human, Mouse, Bovine, Chicken, Feline, Parasite, Porcine.

We have publications tested in 7 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
IB
(1)
ICC/IF
(21)
IHC
(10)
IHC-Fr
(1)
IHC-P
(3)
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(17)
Mouse
(16)
Bovine
(1)
Chicken
(3)
Feline
(1)
Parasite
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 54. Show All 54 Publications.
Publications using NB300-232 Applications Species
Xie Y, Khan R, Wahab F et al. The testis-specifically expressed Dpep3 is not essential for male fertility in mice Gene Jun 15 2019 [PMID: 31212048] (ICC/IF, Mouse) ICC/IF Mouse
Wells D, Bitoun E, Moralli D et al. ZCWPW1 is recruited to recombination hotspots by PRDM9, and is essential for meiotic double strand break repair BioRxiv Oct 30 2019 (Mouse) Mouse
Gorence GJ, Pulcastro HC, Lawson CA et al. Chemical Contaminants from Plastics in the Animal Environment J. Am. Assoc. Lab. Anim. Sci. 2019 Mar 01 [PMID: 30646968] (ICC/IF, Mouse) ICC/IF Mouse
Jorgensen A, Macdonald J, Nielsen JE et al. Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis. Cell Rep. Nov 13 2018 [PMID: 30428358] (IHC, Human) IHC Human
Mu X, Wen J, Chen Q et al. Retinoic acid-induced CYP51 nuclear translocation promotes meiosis prophase I process and is correlated to the expression of REC8 and STAG3 in mice. Biol Open. Nov 12 2018 [PMID: 30420384] (ICC/IF, Mouse) ICC/IF Mouse
Mary N, Villagomez DAF, Revay T et al. Meiotic Synapsis and Gene Expression Altered by a Balanced Y-Autosome Reciprocal Translocation in an Azoospermic Pig. Sex Dev Sep 5 2018 [PMID: 30179878] (ICC/IF, Porcine) ICC/IF Porcine
Zhai QY, Ge W, Wang JJ et al. Exposure to Zinc oxide nanoparticles during pregnancy induces oocyte DNA damage and affects ovarian reserve of mouse offspring. Aging (Albany NY) Aug 28 2018 [PMID: 30153657] (ICC/IF, Mouse) ICC/IF Mouse
Cheng SF, Qin XS, Han ZL et al. Nicotine exposure impairs germ cell development in human fetal ovaries cultured in vitro. Aging (Albany NY). Jul 12 2018 [PMID: 30001218] (ICC/IF, Human) ICC/IF Human
Pang J, Zhou Q, Sun X et al. Effect of low-dose zearalenone exposure on reproductive capacity of male mice Toxicol. Appl. Pharmacol. 2017 Aug 22 [PMID: 28837831] (Mouse) Mouse
Ge W, Wang JJ, Zhang RQ et al. Dissecting the initiation of female meiosis in the mouse at single-cell resolution bioRxiv 2019 Oct 14 (ICC/IF, Mouse) ICC/IF Mouse
Show All 54 Publications.

Reviews for SCP3/SYCP3 Antibody (NB300-232) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 review tested in 1 species: Mouse.

Reviews using NB300-232:
Filter by Applications
ICC
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SCP3/SYCP3 NB300-232
Enlarge
5
reviewed by:
Anonymous
ICC Mouse 11/11/2019
View

Summary

ApplicationImmunocytochemistry
Sample Testedgerm cells
SpeciesMouse
LotH-3
Immunohistochemistry-Paraffin SCP3/SYCP3 NB300-232
Enlarge
4
reviewed by:
Anonymous
IHC-P 07/12/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin
Special ApplicationsThe antibody works in adult marmoset testis but not in neonate nor juvenile testis as spermatogensis has not taken place. However, we only observe the expression in the spermatozoa, which causes a bit concern. This is because that SCP3 was reported to be expressed in the spermatocyte, which was not observed in adult marmoset testis.

Product General Protocols

View specific protocols for SCP3/SYCP3 Antibody (NB300-232): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SCP3/SYCP3 Antibody (NB300-232). (Showing 1 - 5 of 5 FAQs).

  1. What was the dilution used for the CDK2 antibody in the ICC staining?
    • The antibody was used at a dilution of 1:500 for this image. 
  2. Is it a monoclonal antibody or polyclonal antibody?
    • This antibody is a rabbit polyclonal antibody. 
  3. What was the incubation time and temperature for this CDK2 antibody?
    • Primary incubation was performed at 4C overnight
  4. Was a permeabilization step performed for the mouse pachytene cells?
    • Our lab did perform a permeabilization step for this staining. The lab used 0.15% Triton-X in the fixation buffer. The tissues were then incubated for 2 hours.
  5. Could you tell us immunogen sequence of this antibody?
    •  The exact immunogen sequence for our product NB300-232 is proprietary. We can tell you that the immunogen falls between aa185-236 of the human SYCP3 protein (Uniprot Site).

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-232AF350
Alexa Fluor 405 NB300-232AF405
Alexa Fluor 488 NB300-232AF488
Alexa Fluor 532 NB300-232AF532
Alexa Fluor 594 NB300-232AF594
Alexa Fluor 647 NB300-232AF647
Alexa Fluor 700 NB300-232AF700
Alexa Fluor 750 NB300-232AF750
Biotin NB300-232B
DyLight 350 NB300-232UV
DyLight 405 NB300-232V
DyLight 488 NB300-232G
DyLight 550 NB300-232R
DyLight 594 NB300-232DL594
DyLight 650 NB300-232C
DyLight 680 NB300-232FR
DyLight 755 NB300-232IR
FITC NB300-232F
HRP NB300-232H
Janelia Fluor 549 NB300-232JF549
Janelia Fluor 646 NB300-232JF646

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Bioinformatics Tool for SCP3/SYCP3 Antibody (NB300-232)

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Blogs on SCP3/SYCP3.

SCP3: A Key to Meiotic Recombination, Sterility and Cancer
Synaptonemal Complex Protein 3 (SCP3), which is a protein present in the synaptonemal complex which is responsible for pairing, synapsis, and recombination of homologous chromosomes during meiosis. Meiosis, in basic terms, is where germ cells divide t...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
11/11/2019
Application: ICC
Species: Mouse

Anonymous
07/12/2012
Application: IHC-P
Species:

Bioinformatics

Gene Symbol SYCP3
Entrez
Uniprot