SCP3/SYCP3 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Chromosome spread for sycp3. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC analysis of a formalin fixed and paraffin embedded tissue section of Mouse testis using SCP3 antibody at 1:1000 dilution. The signal was detected ...read more
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Exposure to DEHP impairs meiotic progression of oocytes from pachytene to diplotene. Immunolabeling of the oocyte chromosomes with anti-SYCP3 ...read more
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Loss of lamin C2 has no effect on meiotic telomere attachment. Chromosome spread preparations of pachytene-like lamin C2-/- spermatocytes ...read more
Western Blot: SCP3/SYCP3 Antibody [NB300-232] - SCP3 Antibody [NB300-232] - Analysis of SCP3 in mouse testis protein.
Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - Analysis of SCP3 in human testis using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - SCP3 Antibody [NB300-232] - SCP3 labeled in mouse pachytene preparation (red), using NB300-232 SCP3 antibody. CDK2 staining, near teleomeres, is ...read more
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Loss of lamin C2 has no effect on meiotic telomere attachment.. Chromosome spread preparations of pachytene-like lamin C2-/- spermatocytes ...read more
Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC-P analysis of formalin fixed paraffin embedded tissue section of mouse testes using SCP3 antibody at 1:200 dilution. Specific staining may be seen in ...read more
CYP51 regulates the expression of the meiosis-specific cohesin subunits REC8 and STAG3. (A,B) RS21745 treatment decreased REC8 and STAG3 expression. Ovaries at 14.5 dpc were cultured with 10 uM RS21745 for 3 days in ...read more
Exposure to DEHP impairs meiotic progression of oocytes from pachytene to diplotene. (a) Immunolabeling of the oocyte chromosomes with anti-SYCP3 antibody (red) and Hoechst 33342 (blue). Image collected and cropped by ...read more
Dose-dependent decrease or increase of the number of meiotic germ cells (SCP3, MLH1) and of gammaH2AX positive cell, respectively, in nicotine treated fetal ovaries cultured for 4 days. (A) Representative IF images of ...read more
Induction of meiosis is compromised in the cortical germ cells of D17 embryos subject to oestrogen level alterations. (B) Sections from the D17 (HH43) left gonad shown in A immunostained for gammaH2AX (green) and SYCP3 ...read more
NSCs Reduce Succinate Levels and Reprogram the Metabolism of Type 1 Pro-inflammatory Mφ toward Oxidative Phosphorylation In Vitro(A) Experimental setup for in vitro MφLPS co-cultures with iNSCs/NSCs.(B and C) Gene ...read more
Lp(a) upregulates GPx1 and Prdx6 expression in human HepG2 cells. HepG2 cells were treated with 5 μg/mL of Lp(a) or LDL for 6 hours at 37°C. Western blots of cell lysates were performed with an anti-Gpx1 antibody (a), ...read more
Metformin-mediated AMPK activation is involved in suppression of TGF-beta -induced NOX4 expression in LF. a WB using anti-NOX4, and anti-beta -actin of cell lysates from control (lane 1, 2) and metformin (lane 3, 4) ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Fe, PaSpecies Glossary
Applications WB, ICC/IF, IHC, IP, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

SCP3/SYCP3 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to the C-terminal region of the human SCP3 protein. [UniProt# Q8IZU3]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYCP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting reported in scientific literature (PMID 27612028)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen 1:200-1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation reported in scientific literature (PMID 18694876)
  • Western Blot 0.5 ug/ml
Application Notes
In Western blot, a band is seen at ~28 kDa.
Control
SCP3/SYCP3 Overexpression Lysate
Control Peptide
SCP3/SYCP3 Peptide (NB300-232PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-232 in the following applications:

Publications
Read Publications using
NB300-232 in the following applications:

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 26096940). Parasite, and Feline reactivity reported in scientific literature (PMID: 27084479). Human reactivity has been observed in IHC. Human reactivity reported in scientific literature (PMID: 29231814).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SCP3/SYCP3 Antibody - BSA Free

  • anti-SCP3
  • COR1
  • MGC71888
  • SCP3 chromosome 3 open reading frame 8
  • SCP3 COR1
  • SCP3
  • SCP-3
  • SYCP3
  • synaptonemal complex protein 3

Background

SCP3 (Synaptonemal complex protein 3) is a DNA-binding nuclear protein that belongs to the XLR/SYCP3 family and is an essential structural component of the transverse filaments of synaptonemal complexes formed between homologous chromosomes during meiotic prophase. The synaptonemal complex develops during prophase of first meiosis, wherein it allows the synapse as well as recombination between maternal - paternal chromosomes, and SCP3 is essential to hold cohesin complex between homologous chromosomes, which avoid premature segregation thereby preventing aneuploidies. SCP3 is involved in phosphorylation of HORMAD1 and HORMAD2. It is essential to development of testis (where it is primarily localized) and defective SCP3 may leads to spermatogenic failure type 4 (SPGF4). SCP3 deificient mouse depicts high numbers of aneuploidies in oocytes and offspring. SCP3 is also expressed in certain malignancies including human acute lymphatic leukemias, cervical cancer cells, lung cancer cells etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCP3/SYCP3 Antibody (NB300-232)(77)

We have publications tested in 8 confirmed species: Human, Mouse, Bovine, Chicken, Feline, Parasite, Porcine, Zebrafish.

We have publications tested in 8 applications: IB, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
IB
(1)
ICC/IF
(31)
IF/IHC
(13)
IHC
(1)
IHC-Fr
(1)
IHC-P
(4)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(17)
Mouse
(26)
Bovine
(1)
Chicken
(3)
Feline
(1)
Parasite
(1)
Porcine
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 77. Show All 77 Publications.
Publications using NB300-232 Applications Species
Islam KN, Ajao A, Venkataramani K et al. The RNA-binding protein Adad1 is necessary for germ cell maintenance and meiosis in zebrafish PLOS Genetics 2023-08-08 [PMID: 37552671]
Dil S, Ye J, Ma H et al. Cornichon protein CNIH4 is not essential for mice gametogenesis and fertility Developmental biology 2023-01-16 [PMID: 36657507] (ICC/IF, Mouse)

Details:
Dilution used in ICC 1:100
ICC/IF Mouse
Abdallah S, Jampy A, Moison D et al. Foetal exposure to the bisphenols BADGE and BPAF impairs meiosis through DNA oxidation in mouse ovaries Environmental Pollution 2022-12-01 [PMID: 36464114] (IF/IHC, Mouse) IF/IHC Mouse
Gruhn JR. Sex-specific differences in human meiotic recombination: A cytogenetic analysis of early meiotic events. Thesis. 2014-01-01 (ICC/IF, Human) ICC/IF Human
Bailey A, Fuller M YTHDC2 serves a distinct late role in spermatocytes during germ cell differentiation bioRxiv 2023-01-23
Davies B, Zhang G, Moralli D et al. Characterization of meiotic recombination intermediates through gene knockouts in founder hybrid mice Genome research 2023-11-17 [PMID: 37977820] (IHC, Mouse)

Details:
Sample type: Testis
IHC Mouse
He J, Yan A, Chen B et al. 3D genome remodeling and homologous pairing during meiotic prophase of mouse oogenesis and spermatogenesis Developmental cell 2023-11-13 [PMID: 37963468]
Wu D, Huang H, Chen T et al. The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination Cell discovery 2023-10-03 [PMID: 37789001]
Younis N, Caldeira-Brant AL, Chu T et al. Human immature testicular tissue organ culture: a step towards fertility preservation and restoration Front Endocrinol (Lausanne) 2023-08-28 [PMID: 37701899] (ICC/IF) ICC/IF
Show All 77 Publications.

Reviews for SCP3/SYCP3 Antibody (NB300-232) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 review tested in 1 species: Mouse.

Reviews using NB300-232:
Filter by Applications
ICC
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SCP3/SYCP3 NB300-232
Enlarge
5
reviewed by:
Verified Customer
ICC Mouse 11/11/2019
View

Summary

ApplicationImmunocytochemistry
Sample Testedgerm cells
SpeciesMouse
LotH-3
Immunohistochemistry-Paraffin SCP3/SYCP3 NB300-232
Enlarge
4
reviewed by:
Verified Customer
IHC-P 07/12/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SCP3/SYCP3 Antibody (NB300-232). (Showing 1 - 5 of 5 FAQs).

  1. What was the dilution used for the CDK2 antibody in the ICC staining?
    • The antibody was used at a dilution of 1:500 for this image. 
  2. Is it a monoclonal antibody or polyclonal antibody?
    • This antibody is a rabbit polyclonal antibody. 
  3. What was the incubation time and temperature for this CDK2 antibody?
    • Primary incubation was performed at 4C overnight
  4. Was a permeabilization step performed for the mouse pachytene cells?
    • Our lab did perform a permeabilization step for this staining. The lab used 0.15% Triton-X in the fixation buffer. The tissues were then incubated for 2 hours.
  5. Could you tell us immunogen sequence of this antibody?
    •  The exact immunogen sequence for our product NB300-232 is proprietary. We can tell you that the immunogen falls between aa185-236 of the human SYCP3 protein (Uniprot Site).

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional SCP3/SYCP3 Products

Research Areas for SCP3/SYCP3 Antibody (NB300-232)

Find related products by research area.

Blogs on SCP3/SYCP3.

SCP3: A Key to Meiotic Recombination, Sterility and Cancer
Synaptonemal Complex Protein 3 (SCP3), which is a protein present in the synaptonemal complex which is responsible for pairing, synapsis, and recombination of homologous chromosomes during meiosis. Meiosis, in basic terms, is where germ cells divide t...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Verified Customer
11/11/2019
Application: ICC
Species: Mouse

Verified Customer
07/12/2012
Application: IHC-P
Species:

Bioinformatics

Gene Symbol SYCP3
Entrez
Uniprot