SCP3/SYCP3 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Chromosome spread for sycp3. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC analysis of a formalin fixed and paraffin embedded tissue section of Mouse testis using SCP3 antibody at 1:1000 dilution. The signal was detected ...read more
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Exposure to DEHP impairs meiotic progression of oocytes from pachytene to diplotene. Immunolabeling of the oocyte chromosomes with anti-SYCP3 ...read more
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Loss of lamin C2 has no effect on meiotic telomere attachment. Chromosome spread preparations of pachytene-like lamin C2-/- spermatocytes ...read more
Western Blot: SCP3/SYCP3 Antibody [NB300-232] - SCP3 Antibody [NB300-232] - Analysis of SCP3 in mouse testis protein.
Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - Analysis of SCP3 in human testis using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - SCP3 Antibody [NB300-232] - SCP3 labeled in mouse pachytene preparation (red), using NB300-232 SCP3 antibody. CDK2 staining, near teleomeres, is ...read more
Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Loss of lamin C2 has no effect on meiotic telomere attachment.. Chromosome spread preparations of pachytene-like lamin C2-/- spermatocytes ...read more
Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC-P analysis of formalin fixed paraffin embedded tissue section of mouse testes using SCP3 antibody at 1:200 dilution. Specific staining may be seen in ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Fe, PaSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

SCP3/SYCP3 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to the C-terminal region of the human SCP3 protein. [UniProt# Q8IZU3]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYCP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen 1:200-1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation
  • Western Blot 0.5 ug/ml
Application Notes
This SCP3 antibody is useful for Immunofluorescence, Immunohistochemistry (Frozen and Paraffin), Immunocytochemistry and Western blot where a band is seen at ~28 kDa. Use in immunoblotting reported in scientific literature (PMID: 27612028). Use in immunoprecipitation reported in scientific literature (PMID: 18694876).
Control
SCP3/SYCP3 Overexpression Lysate
Control Peptide
SCP3/SYCP3 Peptide (NB300-232PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-232 in the following applications:

Publications
Read Publications using
NB300-232 in the following applications:

  • IB
    1 publication
  • 26 publications
  • IHC
    12 publications
  • 1 publication
  • 4 publications
  • IP
    1 publication
  • WB
    6 publications

Reactivity Notes

Rat, pig, bovine and mouse. Chicken reactivity reported in scientific literature (PMID: 26096940). Parasite, and Feline reactivity reported in scientific literature (PMID: 27084479). Human reactivity has been observed in IHC. Human reactivity reported in scientific literature (PMID: 29231814).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SCP3/SYCP3 Antibody - BSA Free

  • anti-SCP3
  • COR1
  • MGC71888
  • SCP3 chromosome 3 open reading frame 8
  • SCP3 COR1
  • SCP3
  • SCP-3
  • SYCP3
  • synaptonemal complex protein 3

Background

SCP3 (Synaptonemal complex protein 3) is a DNA-binding nuclear protein that belongs to the XLR/SYCP3 family and is an essential structural component of the transverse filaments of synaptonemal complexes formed between homologous chromosomes during meiotic prophase. The synaptonemal complex develops during prophase of first meiosis, wherein it allows the synapse as well as recombination between maternal - paternal chromosomes, and SCP3 is essential to hold cohesin complex between homologous chromosomes, which avoid premature segregation thereby preventing aneuploidies. SCP3 is involved in phosphorylation of HORMAD1 and HORMAD2. It is essential to development of testis (where it is primarily localized) and defective SCP3 may leads to spermatogenic failure type 4 (SPGF4). SCP3 deificient mouse depicts high numbers of aneuploidies in oocytes and offspring. SCP3 is also expressed in certain malignancies including human acute lymphatic leukemias, cervical cancer cells, lung cancer cells etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SCP3/SYCP3 Antibody (NB300-232)(66)

We have publications tested in 8 confirmed species: Human, Mouse, Bovine, Chicken, Feline, Parasite, Porcine, Zebrafish.

We have publications tested in 7 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
IB
(1)
ICC/IF
(26)
IHC
(12)
IHC-Fr
(1)
IHC-P
(4)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(17)
Mouse
(21)
Bovine
(1)
Chicken
(3)
Feline
(1)
Parasite
(1)
Porcine
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 66. Show All 66 Publications.
Publications using NB300-232 Applications Species
Ali H, Unar A, Dil S et al. Testis-specific fascin component FSCN3 is dispensable for mouse spermatogenesis and fertility Molecular biology reports Apr 21 2022 [PMID: 35449315] (ICC/IF, Mouse) ICC/IF Mouse
Li Y, Meng R, Li S et al. The ZFP541-KCTD19 complex is essential for pachytene progression by activating meiotic genes during mouse spermatogenesis Journal of genetics and genomics = Yi chuan xue bao Mar 24 2022 [PMID: 35341968] (IHC, Mouse) IHC Mouse
Tseng H, Liu yL, Lu BJ, Chen CH Immature Testicular Tissue Engineered from Weaned Mice to Adults for Prepubertal Fertility Preservation-An In Vivo Translational Study International journal of molecular sciences Feb 12 2022 [PMID: 35216156] (IHC-P, Mouse) IHC-P Mouse
Gai X, Xin D, Wu D et al. Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response Cell research Jan 4 2022 [PMID: 34980897]
Islam KN, Modi MM, Siegfried KR The Zebrafish Meiotic Cohesin Complex Protein Smc1b Is Required for Key Events in Meiotic Prophase I Frontiers in cell and developmental biology Aug 9 2021 [PMID: 34434933] (ICC/IF, Zebrafish) ICC/IF Zebrafish
Guioli, S, Zhao, D Et al. In the chick embryo, estrogen can induce chromosomally male ZZ left gonad epithelial cells to form an ovarian cortex, which supports oogenesis. Development Jan 1 2020 [PMID: 34004754]
Hassold T, Maylor-Hagen H, Wood A et al. Failure to recombine is a common feature of human oogenesis American journal of human genetics Nov 24 2020 [PMID: 33306948]
Zaman Q, Khan R, Hameed U et al. Exonuclease 5 is dispensable for meiotic progression and male fertility in mouse Gene Oct 1 2020 [PMID: 33164760] (ICC/IF, Mouse) ICC/IF Mouse
Yousaf A, Wu Y, Khan R et al. Normal spermatogenesis and fertility in Ddi1 (DNA damage inducible 1) mutant mice Reprod Biol Oct 19 2020 [PMID: 33092996] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Yun Y, Ito M, Sandhu S, Hunter N Cytological Monitoring of Meiotic Crossovers in Spermatocytes and Oocytes Methods Mol. Biol. Aug 26 2020 [PMID: 32840786]
Show All 66 Publications.

Reviews for SCP3/SYCP3 Antibody (NB300-232) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 review tested in 1 species: Mouse.

Reviews using NB300-232:
Filter by Applications
ICC
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SCP3/SYCP3 NB300-232
Enlarge
5
reviewed by:
Nin Wan
ICC Mouse 11/11/2019
View

Summary

ApplicationImmunocytochemistry
Sample Testedgerm cells
SpeciesMouse
LotH-3
Immunohistochemistry-Paraffin SCP3/SYCP3 NB300-232
Enlarge
4
reviewed by:
Zachary Yu-Ching Lin
IHC-P 07/12/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SCP3/SYCP3 Antibody (NB300-232). (Showing 1 - 5 of 5 FAQs).

  1. What was the dilution used for the CDK2 antibody in the ICC staining?
    • The antibody was used at a dilution of 1:500 for this image. 
  2. Is it a monoclonal antibody or polyclonal antibody?
    • This antibody is a rabbit polyclonal antibody. 
  3. What was the incubation time and temperature for this CDK2 antibody?
    • Primary incubation was performed at 4C overnight
  4. Was a permeabilization step performed for the mouse pachytene cells?
    • Our lab did perform a permeabilization step for this staining. The lab used 0.15% Triton-X in the fixation buffer. The tissues were then incubated for 2 hours.
  5. Could you tell us immunogen sequence of this antibody?
    •  The exact immunogen sequence for our product NB300-232 is proprietary. We can tell you that the immunogen falls between aa185-236 of the human SYCP3 protein (Uniprot Site).

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SCP3/SYCP3 Antibody (NB300-232)

Discover related pathways, diseases and genes to SCP3/SYCP3 Antibody (NB300-232). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on SCP3/SYCP3.

SCP3: A Key to Meiotic Recombination, Sterility and Cancer
Synaptonemal Complex Protein 3 (SCP3), which is a protein present in the synaptonemal complex which is responsible for pairing, synapsis, and recombination of homologous chromosomes during meiosis. Meiosis, in basic terms, is where germ cells divide t...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Nin Wan
11/11/2019
Application: ICC
Species: Mouse

Zachary Yu-Ching Lin
07/12/2012
Application: IHC-P
Species:

Bioinformatics

Gene Symbol SYCP3
Entrez
Uniprot