Rat ZP2 - Ready-To-Use ELISA Kit (Colorimetric)

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.41 ng/mL (example only; lot dependent) (example only; lot dependent)

Rat ZP2 - Ready-To-Use ELISA Kit (Colorimetric) Summary

• Description: The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
  Assay Length: 3 hours
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.41 ng/mL (example only; lot dependent) (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ZP2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat ZP2 - Ready-To-Use ELISA Kit (Colorimetric)

• zona pellucida glycoprotein 2 (sperm receptor)
• Zona pellucida glycoprotein 2
• zona pellucida glycoprotein ZP2
• Zona Pellucida Protein A
• zona pellucida sperm-binding protein 2
• ZP2
• Zp-2
• ZPA

Background

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for ZP2 ELISA Kit (NBP3-55774). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ZP2