Rad51D Antibody (5B3/6) - BSA Free

Western Blot: Rad51D Antibody (5B3/6) [NB100-178] - Rad51D detected in HEK293 lysates using a 1:1,000 dilution of NB 100-178 (purified). Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical School

Immunocytochemistry/Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178] - RAD51D antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

• Reactivity: Hu, Mu(-)
• Applications: WB, ICC/IF
• Clone: 5B3/6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Rad51D Antibody (5B3/6) - BSA Free Summary

• Immunogen: His-tagged human Rad51D, overexpressed in E. coli. [UniProt# O75771]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD51D
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Western Blot 1:1000
• Application Notes: This Rad51D (5B3/6) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~40 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Rad51D Overexpression Lysate

Reactivity Notes

This antibody does not work on mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Rad51D Antibody (5B3/6) - BSA Free

• DNA repair protein RAD51 homolog 4
• HsTRAD
• R51H3Trad
• RAD51 homolog D
• RAD51DRAD51 (S. cerevisiae)-like 3
• RAD51-like 3 (S. cerevisiae)
• RAD51-like protein 3
• recombination repair protein
• TRAD

Background

Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. RAD51D interacts with XRCC2 and RAD51C (1). RAD51D's expression pattern and sequence similarity to other RAD51 family members most likely make it part of a complex of proteins required for DNA repair and meiotic recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad51D Antibody (NB100-178)(3)

Publications using NB100-178

• Shammas MA, Shmookler Reis RJ, Koley H et al. Dysfunctional homologous recombination mediates genomic instability progression in myeloma. Blood 2009;113(10):2290-2297 [PMID: 19050310] (ICC/IF, Human)
• Hinz, JM et al. Repression of mutagenesis by Rad51D-mediated homologous recombination. Nucleic Acids Res;34(5):1358-68. 2006-03-06 [PMID: 16522646]
• Fan, R et al. Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression. Cancer Res;64(23):8526-33. 2004-12-01 [PMID: 15574758] (WB, Human)

FAQs for Rad51D Antibody (NB100-178). (Showing 1 - 1 of 1 FAQs).

1. What is the sodium azide percentage for this product?
   • 0.1% sodium azide 

Bioinformatics

• Gene Symbol: RAD51D