Rad51D Antibody (5B3/6)

Images

 
Western Blot: Rad51D Antibody (5B3/6) [NB100-178] - Rad51D detected in HEK293 lysates using a 1:1,000 dilution of NB 100-178 (purified). Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical ...read more
Immunocytochemistry/ Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178] - RAD51D antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, ICC/IF
Clone
5B3/6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Rad51D Antibody (5B3/6) Summary

Immunogen
His-tagged human Rad51D, overexpressed in E. coli. [UniProt# O75771]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAD51D
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
Application Notes
This Rad51D (5B3/6) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~40 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Rad51D Overexpression Lysate
Publications
Read Publications using
NB100-178 in the following applications:

Reactivity Notes

Human. This antibody does not work on mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Rad51D Antibody (5B3/6)

  • DNA repair protein RAD51 homolog 4
  • HsTRAD
  • R51H3Trad
  • RAD51 homolog D
  • RAD51DRAD51 (S. cerevisiae)-like 3
  • RAD51-like 3 (S. cerevisiae)
  • RAD51-like protein 3
  • recombination repair protein
  • TRAD

Background

Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. RAD51D interacts with XRCC2 and RAD51C (1). RAD51D's expression pattern and sequence similarity to other RAD51 family members most likely make it part of a complex of proteins required for DNA repair and meiotic recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad51D Antibody (NB100-178)(3)

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Product General Protocols

View specific protocols for Rad51D Antibody (NB100-178): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rad51D Antibody (NB100-178). (Showing 1 - 1 of 1 FAQs).

  1. What is the sodium azide percentage for this product?
    • 0.1% sodium azide 

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol RAD51D