XRCC3 Overexpression Lysate (Native)

Images

 
Western Blot: XRCC3 Overexpression Lysate (Adult Normal) [NBL1-17915] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XRCC3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

XRCC3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200326 and based on accession number NM_005432. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2, mRNA.
Gene
XRCC3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
37.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for XRCC3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for XRCC3 Overexpression Lysate (Native)

  • CMM6
  • DNA repair protein XRCC3
  • X-ray repair complementing defective repair in Chinese hamster cells 3
  • X-ray repair cross-complementing protein 3

Background

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]. Transcript Variant: This variant (2) lacks a segment in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-02280
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF3296
Species: Hu, Mu, Rt
Applications: WB
NB100-148
Species: Hu, Mu, Ce, Ch
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro
H00007516-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
NB100-177
Species: Hu, Mu, Pm, Ye
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NB100-178
Species: Hu, Mu(-)
Applications: WB, ICC/IF
NB100-143
Species: Hu, Mu, Ha, Ma, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-P, IP, KO
NB100-176
Species: Hu, Pm, Mu(-)
Applications: WB, ICC/IF
NB100-106
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
MAB2476
Species: Hu
Applications: WB, IHC
NB600-326
Species: NA
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP
NB500-704
Species: Hu, Rt, ChHa
Applications: WB, ICC/IF, IHC, IHC-P
NB100-74611
Species: Hu
Applications: WB, IHC-P, IP
NBP2-01020
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-56155
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-477
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-104
Species: Hu, Mu
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
NBP1-32733
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP

Publications for XRCC3 Lysate (NBL1-17915) (0)

There are no publications for XRCC3 Lysate (NBL1-17915).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for XRCC3 Lysate (NBL1-17915) (0)

There are no reviews for XRCC3 Lysate (NBL1-17915). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for XRCC3 Lysate (NBL1-17915) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional XRCC3 Products

Bioinformatics Tool for XRCC3 Lysate (NBL1-17915)

Discover related pathways, diseases and genes to XRCC3 Lysate (NBL1-17915). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for XRCC3 Lysate (NBL1-17915)

Discover more about diseases related to XRCC3 Lysate (NBL1-17915).
 

Pathways for XRCC3 Lysate (NBL1-17915)

View related products by pathway.

PTMs for XRCC3 Lysate (NBL1-17915)

Learn more about PTMs related to XRCC3 Lysate (NBL1-17915).
 

Research Areas for XRCC3 Lysate (NBL1-17915)

Find related products by research area.

Blogs on XRCC3

There are no specific blogs for XRCC3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Nbs1 Antibody
NB100-143
XRCC3 Antibody
NB100-165
ATM Antibody
NB100-104

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our XRCC3 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol XRCC3